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The map label for this gene is recF

Identifier: 116493578

GI number: 116493578

Start: 3529

End: 4644

Strand: Direct

Name: recF

Synonym: LSEI_0004

Alternate gene names: 116493578

Gene position: 3529-4644 (Clockwise)

Preceding gene: 116493577

Following gene: 116493579

Centisome position: 0.12

GC content: 46.33

Gene sequence:

>1116_bases
ATGAAACTGGATCACTTGGTGCTGAAAAATTATCGCAATTATGCTGCTGTTGATACAACATTTTCACCAGAAATCAATGT
TTTGATTGGGGCAAACGCTCAAGGCAAGACGAATCTGCTTGAATCAATCTATGTTTTGGCATTGGCTCGCAGTCATCGAA
CCAACAATGATAAGGAACTGATTCGCTTTGGTAGCGAGTTTGCACGGGTTTCCGGGCAAGTTTCGCGACAAAGCGGTTCC
CATCAGTTGGAGTTGATTATCAGTCATCAAGGCAAGCGTGCCCGGATTGATCGCATCGAACAACCGAAGCTTTCCCAGTA
TTTAGGGCATTTTAATGTCATCTTGTTTGCACCTGAGGATTTAGCCATTGTCAAGGGCAGTCCGGCTGGGCGGCGTCGCT
TCATCGACATGGAGTTTGGCCAGATGAGTCCCAAGTACCTTTATAATCTGAGCCAATATAAAACGTTTTTGAAGCAGCGC
AATGCGTATTTGAAGCAGCTTAAGTATCATCAGGCCAAGGATCTGGTTTATCTGGATGTGTTGACTGACTCGCTTGCAGC
TTTCGGGGCAGAGCTAATTACGGCGCGCGCTAAGTTGTTGGAGACGATGTCGGATTATGCTGCAACGATTCAGCAAGATA
TCACCAAGGGGCGGGAGTCATTACATTTTTCTTATCAAACACAGGTTGACCCCAGTCTTCGCGGTAACAGTGAGCAGGTT
TACACTGCTTTGGGCGAGATGTTTGCCAAGCAGCAAGCGCGCGAAATCGAACAAGGAACCAGTTTAGTCGGTCCCCAGCG
AGATGATGTGTTGTTCATCGTCAATGATAAGGATGTTGCCAATTTTGGCAGTCAAGGGCAACAACGCACCACAGCATTGG
CTGTTAAGCTGGCAGAGATTGACCTTATGAAGGATCAAACCGGCGAATATCCAGTGCTATTACTGGACGATGTGTTGTCA
GAATTAGATGCGGCTCGCCAAACCCATTTACTTAAGGCCATTCAAACCAAAGTCCAAACCTTTTTAACCACCACAAGTTT
GGAAGGCATTCAAAAAGAAATCATCGCAACCCCTGCAGTCTTTAAAGTTGATGAGGGGACATTAGCAAGAGCGTGA

Upstream 100 bases:

>100_bases
CAGTGACAGTCAACGGCGCAGCTGAGGAGCGACGTGGGCGCAAGCTGTATCCAGATGATCAAGTCAGCGTGGCCGGCCAA
ACATACGTGATCGTGAGCGC

Downstream 100 bases:

>100_bases
ACCAGCCCGGTTAGAAACCGGAGTGTAAGTGGGCCTGAACGTGATGACCGTACTTTGGTCATTGCGTTCAGGTTCCTTAC
ACGCAGGTTTCTGGGCTGGT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKLDHLVLKNYRNYAAVDTTFSPEINVLIGANAQGKTNLLESIYVLALARSHRTNNDKELIRFGSEFARVSGQVSRQSGS
HQLELIISHQGKRARIDRIEQPKLSQYLGHFNVILFAPEDLAIVKGSPAGRRRFIDMEFGQMSPKYLYNLSQYKTFLKQR
NAYLKQLKYHQAKDLVYLDVLTDSLAAFGAELITARAKLLETMSDYAATIQQDITKGRESLHFSYQTQVDPSLRGNSEQV
YTALGEMFAKQQAREIEQGTSLVGPQRDDVLFIVNDKDVANFGSQGQQRTTALAVKLAEIDLMKDQTGEYPVLLLDDVLS
ELDAARQTHLLKAIQTKVQTFLTTTSLEGIQKEIIATPAVFKVDEGTLARA

Sequences:

>Translated_371_residues
MKLDHLVLKNYRNYAAVDTTFSPEINVLIGANAQGKTNLLESIYVLALARSHRTNNDKELIRFGSEFARVSGQVSRQSGS
HQLELIISHQGKRARIDRIEQPKLSQYLGHFNVILFAPEDLAIVKGSPAGRRRFIDMEFGQMSPKYLYNLSQYKTFLKQR
NAYLKQLKYHQAKDLVYLDVLTDSLAAFGAELITARAKLLETMSDYAATIQQDITKGRESLHFSYQTQVDPSLRGNSEQV
YTALGEMFAKQQAREIEQGTSLVGPQRDDVLFIVNDKDVANFGSQGQQRTTALAVKLAEIDLMKDQTGEYPVLLLDDVLS
ELDAARQTHLLKAIQTKVQTFLTTTSLEGIQKEIIATPAVFKVDEGTLARA
>Mature_371_residues
MKLDHLVLKNYRNYAAVDTTFSPEINVLIGANAQGKTNLLESIYVLALARSHRTNNDKELIRFGSEFARVSGQVSRQSGS
HQLELIISHQGKRARIDRIEQPKLSQYLGHFNVILFAPEDLAIVKGSPAGRRRFIDMEFGQMSPKYLYNLSQYKTFLKQR
NAYLKQLKYHQAKDLVYLDVLTDSLAAFGAELITARAKLLETMSDYAATIQQDITKGRESLHFSYQTQVDPSLRGNSEQV
YTALGEMFAKQQAREIEQGTSLVGPQRDDVLFIVNDKDVANFGSQGQQRTTALAVKLAEIDLMKDQTGEYPVLLLDDVLS
ELDAARQTHLLKAIQTKVQTFLTTTSLEGIQKEIIATPAVFKVDEGTLARA

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=379, Percent_Identity=27.7044854881267, Blast_Score=128, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_LACC3 (Q03D52)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_805312.1
- STRING:   Q03D52
- GeneID:   4419511
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0004
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- PhylomeDB:   Q03D52
- ProtClustDB:   CLSK2317530
- BioCyc:   LCAS321967:LSEI_0004-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 41633; Mature: 41633

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDHLVLKNYRNYAAVDTTFSPEINVLIGANAQGKTNLLESIYVLALARSHRTNNDKEL
CCCHHHHHHCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
IRFGSEFARVSGQVSRQSGSHQLELIISHQGKRARIDRIEQPKLSQYLGHFNVILFAPED
HHHHHHHHHHHCHHHHCCCCCEEEEEEECCCCCCHHCCCCCCHHHHHHCCEEEEEECCCC
LAIVKGSPAGRRRFIDMEFGQMSPKYLYNLSQYKTFLKQRNAYLKQLKYHQAKDLVYLDV
EEEEECCCCCCEEEEECHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHH
LTDSLAAFGAELITARAKLLETMSDYAATIQQDITKGRESLHFSYQTQVDPSLRGNSEQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHH
YTALGEMFAKQQAREIEQGTSLVGPQRDDVLFIVNDKDVANFGSQGQQRTTALAVKLAEI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHHHH
DLMKDQTGEYPVLLLDDVLSELDAARQTHLLKAIQTKVQTFLTTTSLEGIQKEIIATPAV
HHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
FKVDEGTLARA
EEECCCCCCCC
>Mature Secondary Structure
MKLDHLVLKNYRNYAAVDTTFSPEINVLIGANAQGKTNLLESIYVLALARSHRTNNDKEL
CCCHHHHHHCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
IRFGSEFARVSGQVSRQSGSHQLELIISHQGKRARIDRIEQPKLSQYLGHFNVILFAPED
HHHHHHHHHHHCHHHHCCCCCEEEEEEECCCCCCHHCCCCCCHHHHHHCCEEEEEECCCC
LAIVKGSPAGRRRFIDMEFGQMSPKYLYNLSQYKTFLKQRNAYLKQLKYHQAKDLVYLDV
EEEEECCCCCCEEEEECHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHH
LTDSLAAFGAELITARAKLLETMSDYAATIQQDITKGRESLHFSYQTQVDPSLRGNSEQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHHH
YTALGEMFAKQQAREIEQGTSLVGPQRDDVLFIVNDKDVANFGSQGQQRTTALAVKLAEI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHHHH
DLMKDQTGEYPVLLLDDVLSELDAARQTHLLKAIQTKVQTFLTTTSLEGIQKEIIATPAV
HHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
FKVDEGTLARA
EEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA