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The map label for this gene is def [H]

Identifier: 116493404

GI number: 116493404

Start: 1656643

End: 1657053

Strand: Direct

Name: def [H]

Synonym: PEPE_1676

Alternate gene names: 116493404

Gene position: 1656643-1657053 (Clockwise)

Preceding gene: 116493402

Following gene: 116493414

Centisome position: 90.41

GC content: 37.23

Gene sequence:

>411_bases
ATGATTAAGAGTGTAATTCATGATCAACAAATTTTATCTCAAACGTCAATTCCCGCTACCAAACAAGATTTATCCGTAGT
AATGGATTTGATGGATACTTTAAAAGCCAATAGCGAACGTTGCGTGGGAATGGCCGCTAACATGATTGGCGTTCATAAAA
GAATCATCGTTGTCACTATGGGAATGATGAACGTCCCCATGATCAATCCTGTCATTCTCAAAAAGGGTAATCCATATACC
ACTGAAGAAGGCTGCCTGTCATTAACCGGTGAACGTTCCACCACTCGTTACGACGAAATCGAAGTTCAGTATCTTGATTT
AGAATTTAAAAAACATACCCAAGAATTTAAAGAATTCATCGCCCAAATTATTCAACATGAAGTTGATCATTGTGATGGGA
TTATTATTTAG

Upstream 100 bases:

>100_bases
GCGACTCGATTTATTTACATTCATGCTACTTGCCTAGCCTTTGAATGTCAAGGTTCTAAAAATGGTAAAATGGTGTTATA
AATTAATTAAGGAGCTCAAC

Downstream 100 bases:

>100_bases
TTTTGGAGAGCTAGCATTAATTTGCTAGTTTTTTAATTTATCACCAGTTTATTCCAAACAAAAAAGCCCATCCATTTTAG
GGGGCTCCTCCATTAAATAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MIKSVIHDQQILSQTSIPATKQDLSVVMDLMDTLKANSERCVGMAANMIGVHKRIIVVTMGMMNVPMINPVILKKGNPYT
TEEGCLSLTGERSTTRYDEIEVQYLDLEFKKHTQEFKEFIAQIIQHEVDHCDGIII

Sequences:

>Translated_136_residues
MIKSVIHDQQILSQTSIPATKQDLSVVMDLMDTLKANSERCVGMAANMIGVHKRIIVVTMGMMNVPMINPVILKKGNPYT
TEEGCLSLTGERSTTRYDEIEVQYLDLEFKKHTQEFKEFIAQIIQHEVDHCDGIII
>Mature_136_residues
MIKSVIHDQQILSQTSIPATKQDLSVVMDLMDTLKANSERCVGMAANMIGVHKRIIVVTMGMMNVPMINPVILKKGNPYT
TEEGCLSLTGERSTTRYDEIEVQYLDLEFKKHTQEFKEFIAQIIQHEVDHCDGIII

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=112, Percent_Identity=33.9285714285714, Blast_Score=63, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 15394; Mature: 15394

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSVIHDQQILSQTSIPATKQDLSVVMDLMDTLKANSERCVGMAANMIGVHKRIIVVTM
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEE
GMMNVPMINPVILKKGNPYTTEEGCLSLTGERSTTRYDEIEVQYLDLEFKKHTQEFKEFI
CHHCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHH
AQIIQHEVDHCDGIII
HHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIKSVIHDQQILSQTSIPATKQDLSVVMDLMDTLKANSERCVGMAANMIGVHKRIIVVTM
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEE
GMMNVPMINPVILKKGNPYTTEEGCLSLTGERSTTRYDEIEVQYLDLEFKKHTQEFKEFI
CHHCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHH
AQIIQHEVDHCDGIII
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9572951 [H]