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The map label for this gene is ndoA [H]

Identifier: 116493285

GI number: 116493285

Start: 1526943

End: 1527305

Strand: Reverse

Name: ndoA [H]

Synonym: PEPE_1557

Alternate gene names: 116493285

Gene position: 1527305-1526943 (Counterclockwise)

Preceding gene: 116493286

Following gene: 116493281

Centisome position: 83.35

GC content: 41.6

Gene sequence:

>363_bases
GTGGACGAGTCGAAGATAAGACGCGGTGATTTATTCTATGCAGACTTGTCACCAGTTGTTGGTTCTGAACAGGGCGGGAT
GCGACCGGTTCTTGTTATCCAGAATAACATCGGTAATCACTATAGTCCGACGGTCATTATTGCAGCAATAACGGCTAAAA
TTTCTAAGCCTAAAATGCCTACCCATGTAGGAATTAAAGGTGGAGAACAAGGAGTAGGCCGTAATTCAGTTATTTTAATG
GAACAGATTCGAACAATTGATAAACAACGTCTACATGATAAAATTGGTCGATTAGATGACCAGAAGATGAAACAGGTGGA
CGAGGCCTTAAAGGTAAGTATAGGTCTCGTACCGAATACATAG

Upstream 100 bases:

>100_bases
TTACTCATGACTTTTCTTCATGTGAAGCGGATTGTGAAAAGCATGTGCTTGAACGTTACTAAGTTAAATTTTTTAAATTA
AAAGTTAATGGGGTGACACT

Downstream 100 bases:

>100_bases
AATAGAAAATGTGAACAAAGTTTCACGCTCAAATTTTAAAACCACCTATTTCAAGATAGAAAAAAGGATTCGCAAACTGC
GAATCCTTTTTTATTTACGA

Product: toxin-antitoxin addiction module toxin component MazF (an endoRNAse)

Products: NA

Alternate protein names: Endoribonuclease EndoA; Toxin EndoA [H]

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MDESKIRRGDLFYADLSPVVGSEQGGMRPVLVIQNNIGNHYSPTVIIAAITAKISKPKMPTHVGIKGGEQGVGRNSVILM
EQIRTIDKQRLHDKIGRLDDQKMKQVDEALKVSIGLVPNT

Sequences:

>Translated_120_residues
MDESKIRRGDLFYADLSPVVGSEQGGMRPVLVIQNNIGNHYSPTVIIAAITAKISKPKMPTHVGIKGGEQGVGRNSVILM
EQIRTIDKQRLHDKIGRLDDQKMKQVDEALKVSIGLVPNT
>Mature_120_residues
MDESKIRRGDLFYADLSPVVGSEQGGMRPVLVIQNNIGNHYSPTVIIAAITAKISKPKMPTHVGIKGGEQGVGRNSVILM
EQIRTIDKQRLHDKIGRLDDQKMKQVDEALKVSIGLVPNT

Specific function: Part of a toxin-antitoxin (TA) module. Has specificity for UAC sequences, yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single

COG id: COG2337

COG function: function code T; Growth inhibitor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemK/MazF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003477
- InterPro:   IPR011067 [H]

Pfam domain/function: PF02452 PemK [H]

EC number: NA

Molecular weight: Translated: 13173; Mature: 13173

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDESKIRRGDLFYADLSPVVGSEQGGMRPVLVIQNNIGNHYSPTVIIAAITAKISKPKMP
CCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEEHHCCCCCCC
THVGIKGGEQGVGRNSVILMEQIRTIDKQRLHDKIGRLDDQKMKQVDEALKVSIGLVPNT
CEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure
MDESKIRRGDLFYADLSPVVGSEQGGMRPVLVIQNNIGNHYSPTVIIAAITAKISKPKMP
CCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEEEEHHCCCCCCC
THVGIKGGEQGVGRNSVILMEQIRTIDKQRLHDKIGRLDDQKMKQVDEALKVSIGLVPNT
CEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]