Click here to switch to the map view.

The map label for this gene is pth

Identifier: 116493281

GI number: 116493281

Start: 1522563

End: 1523120

Strand: Reverse

Name: pth

Synonym: PEPE_1553

Alternate gene names: 116493281

Gene position: 1523120-1522563 (Counterclockwise)

Preceding gene: 116493285

Following gene: 116493280

Centisome position: 83.12

GC content: 36.2

Gene sequence:

>558_bases
ATGAAAATGATTGTGGGTTTAGGAAATATTGGTAAAGAATATGATAAAACGCGCCATAATACTGGTTTTATGGTTGTCGA
TGAATTAGCAAAAAAGCATGATATAAATAATTTTAAAGTTCAAAGTGATGCCTTGATCGCAGATTTTCGAGTAAATGGTG
AAAAAGTTTTGTTAGTAAAACCAACTACTTATATGAACGATTCTGGTCGAGCAGTGCGTCCTTTAATGGATTACTACGAT
GTTGATTTAAATGACATGATTGTGGCCTATGATGACATGGATATGCCAGTTGGTAAAATTCGACTTCGTCAAAAGGGCTC
AGCCGGGGGACATAATGGGATTAAAAGTATAATAGCCCATGTAGGAACCCAATCCTTTAATCGAGTGCGAGTAGGGATTG
ATCATCCGACTAAAGAAAGCGTCGTGGATTACGTACTAGGTAAGTTTAGGAAGGAACAGATAACCGATTTTGAAATTGGT
GTTCAAAATGCAGTAGCAGCATTAGAAGATTGGACTACAATTGAAAATTTCAGTCAGCTAATGAATAAATACAACTAG

Upstream 100 bases:

>100_bases
GGGGTCAATAATATGGTAATATTACATGAAAATAAACGTGGACAGCTGAGGGGTTTTTGCTCTTTCAGCCGTTTTTGCGT
GTTGAGGGTGGAAAAATAAC

Downstream 100 bases:

>100_bases
GATTATTTAGGAGCAAGTATGAATATAAATGAAATTTTTGCATCTGATTCGGGAGTTACAACCGTTGTTGATGCTCTCCA
AAAAGGGTCGCGGCAATTAG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKMIVGLGNIGKEYDKTRHNTGFMVVDELAKKHDINNFKVQSDALIADFRVNGEKVLLVKPTTYMNDSGRAVRPLMDYYD
VDLNDMIVAYDDMDMPVGKIRLRQKGSAGGHNGIKSIIAHVGTQSFNRVRVGIDHPTKESVVDYVLGKFRKEQITDFEIG
VQNAVAALEDWTTIENFSQLMNKYN

Sequences:

>Translated_185_residues
MKMIVGLGNIGKEYDKTRHNTGFMVVDELAKKHDINNFKVQSDALIADFRVNGEKVLLVKPTTYMNDSGRAVRPLMDYYD
VDLNDMIVAYDDMDMPVGKIRLRQKGSAGGHNGIKSIIAHVGTQSFNRVRVGIDHPTKESVVDYVLGKFRKEQITDFEIG
VQNAVAALEDWTTIENFSQLMNKYN
>Mature_185_residues
MKMIVGLGNIGKEYDKTRHNTGFMVVDELAKKHDINNFKVQSDALIADFRVNGEKVLLVKPTTYMNDSGRAVRPLMDYYD
VDLNDMIVAYDDMDMPVGKIRLRQKGSAGGHNGIKSIIAHVGTQSFNRVRVGIDHPTKESVVDYVLGKFRKEQITDFEIG
VQNAVAALEDWTTIENFSQLMNKYN

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=151, Percent_Identity=31.7880794701987, Blast_Score=92, Evalue=3e-19,
Organism=Escherichia coli, GI1787455, Length=181, Percent_Identity=37.0165745856354, Blast_Score=100, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_PEDPA (Q03DZ8)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_805016.1
- ProteinModelPortal:   Q03DZ8
- SMR:   Q03DZ8
- STRING:   Q03DZ8
- GeneID:   4417185
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1553
- NMPDR:   fig|278197.10.peg.1362
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   NESGRQV
- ProtClustDB:   PRK05426
- BioCyc:   PPEN278197:PEPE_1553-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 20887; Mature: 20887

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMIVGLGNIGKEYDKTRHNTGFMVVDELAKKHDINNFKVQSDALIADFRVNGEKVLLVK
CEEEEECCCCCHHHHHHCCCCCCEEHHHHHHHCCCCCEEEECCCEEEEEEECCCEEEEEC
PTTYMNDSGRAVRPLMDYYDVDLNDMIVAYDDMDMPVGKIRLRQKGSAGGHNGIKSIIAH
CCEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHEEEECCCCCCCHHHHHHHHHH
VGTQSFNRVRVGIDHPTKESVVDYVLGKFRKEQITDFEIGVQNAVAALEDWTTIENFSQL
HCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
MNKYN
HHHCC
>Mature Secondary Structure
MKMIVGLGNIGKEYDKTRHNTGFMVVDELAKKHDINNFKVQSDALIADFRVNGEKVLLVK
CEEEEECCCCCHHHHHHCCCCCCEEHHHHHHHCCCCCEEEECCCEEEEEEECCCEEEEEC
PTTYMNDSGRAVRPLMDYYDVDLNDMIVAYDDMDMPVGKIRLRQKGSAGGHNGIKSIIAH
CCEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHEEEECCCCCCCHHHHHHHHHH
VGTQSFNRVRVGIDHPTKESVVDYVLGKFRKEQITDFEIGVQNAVAALEDWTTIENFSQL
HCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
MNKYN
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA