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The map label for this gene is tilS [H]

Identifier: 116493275

GI number: 116493275

Start: 1514730

End: 1516100

Strand: Reverse

Name: tilS [H]

Synonym: PEPE_1547

Alternate gene names: 116493275

Gene position: 1516100-1514730 (Counterclockwise)

Preceding gene: 116493276

Following gene: 116493274

Centisome position: 82.74

GC content: 37.71

Gene sequence:

>1371_bases
ATGAAAATCGGTGAGCTAGGTGATCAGATTGTTGAACAATATGGACTGAGCCAATTCAAAATCGTAGTGGCAGTTTCAAC
GGGAGTCGATTCAACGGTGCTAATTAACGCATTGATAAATTCAACTAAAATTAAGCATCAACAATTGATTGTTGCCCATG
TCAATCATCAGCTGCGTGAGCAAAGTATTGAAGAAGAACGATTTTTACATAAATGGTGTCGACAGCATCAAATCACGTTT
ATGGAGACGGACTGGCCTAAAAGTCAGCACCCTCAACATGGAGTAGAAGCTGCTGCACGCGAATTTAGATATACTTTTTT
TAAGCAGGTAATGCATGACCAGCATGCGCAAGTACTATTGACTGCACATAATGCGGATGAGCAAGCTGAAAATTTCGTAA
TGCAAGTGATTCGGGGGGGCTGGCTAAATCAATTAAATGGAATTCCAGAACGGCGAACGTTTGCACAAGGATGGCTAATT
CGCCCGTTTCTAAAATTTACTAAGCAATTTTTGATTGAGACCGCTCAAGAAAATAAATTACGGTGGTTTGAAGATCAAAC
CAACCGTACTACTGAATTTTTACGTAACCGTGTGCGAAATGAAATCATTCCTGCTTTTCGAAAAGAAAATCCAAGGGTGG
TTGAACATATCCAGCACTATCAAGATCAGCTATTAGAACAGTCTAACTTAGTCGACCAAGTGGTTCTTGAAAAACTTACT
AAGTTACAAAGAGATGAAAGCTTTGATCTGCTGGCACTACGTAAAGAATCGCTCCCATGGCAGAAAAAAATTATTTTAAA
AATTATTACTGAAGTTAATGCGGAAATTGCAATAAATGAGAATAAGTTGGATGAGCTAGTTCGATTGGTTAATAATACTA
AACAAGCTCAAGCTACAATCGACATTGGAAAAAACTATAAATTTGAAATCACGTACAATTATCTACGAATTGGTAAAAAA
ATAACCGTAAAAGGTGAACAAGTAAAAAAGCGTGTGGTAACATTGAATTCGTGGTTAAATTTAGATGGTGATGCTCAATT
CCAATTATCTAATCAAAAGTCGGCTTTGGATGCTGACGAGACGATGGTTTTGAGCGCCAATCAGCTGAGGTTCCCGCTTT
TTATCCGGAAAATAAACCCCAACGACCGCTTAAGACTTAAAAATGGTGGTTATAAAACAATTCGTCGAGAGTTAATTGAT
CAAAAGGTTCCGAATGAACAACGAGAACGTTATTTTGCAATTACGGATGTTGATGATGAAATTTTATGGGTACCAGGTTT
GCGAAAGGCCTGGTTAAAGGAACCTTTCCTAAAAGATAATCAACAATTTATTATGGAATTTAGAATTGGAGGAAGTCCCC
ATGGACAATGA

Upstream 100 bases:

>100_bases
TGCTCTCTGGATTTTTAAAGGATAGTGAGTCACGCTTATCAACGTTAAAACGTAATACGGATGGTAAACGTGGTGGCCGT
GGCGGACGCCGTAGTTAACT

Downstream 100 bases:

>100_bases
TATCGCCAGAGTTTTGTACAGTAAGGCGGATATAAAGGAAGCTTGCGAACGTTTAGGTAAACAATTAACAAAGGACTATG
CAGGAAAACGTCCTTTAATC

Product: tRNA(Ile)-lysidine synthetase, MesJ

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MKIGELGDQIVEQYGLSQFKIVVAVSTGVDSTVLINALINSTKIKHQQLIVAHVNHQLREQSIEEERFLHKWCRQHQITF
METDWPKSQHPQHGVEAAAREFRYTFFKQVMHDQHAQVLLTAHNADEQAENFVMQVIRGGWLNQLNGIPERRTFAQGWLI
RPFLKFTKQFLIETAQENKLRWFEDQTNRTTEFLRNRVRNEIIPAFRKENPRVVEHIQHYQDQLLEQSNLVDQVVLEKLT
KLQRDESFDLLALRKESLPWQKKIILKIITEVNAEIAINENKLDELVRLVNNTKQAQATIDIGKNYKFEITYNYLRIGKK
ITVKGEQVKKRVVTLNSWLNLDGDAQFQLSNQKSALDADETMVLSANQLRFPLFIRKINPNDRLRLKNGGYKTIRRELID
QKVPNEQRERYFAITDVDDEILWVPGLRKAWLKEPFLKDNQQFIMEFRIGGSPHGQ

Sequences:

>Translated_456_residues
MKIGELGDQIVEQYGLSQFKIVVAVSTGVDSTVLINALINSTKIKHQQLIVAHVNHQLREQSIEEERFLHKWCRQHQITF
METDWPKSQHPQHGVEAAAREFRYTFFKQVMHDQHAQVLLTAHNADEQAENFVMQVIRGGWLNQLNGIPERRTFAQGWLI
RPFLKFTKQFLIETAQENKLRWFEDQTNRTTEFLRNRVRNEIIPAFRKENPRVVEHIQHYQDQLLEQSNLVDQVVLEKLT
KLQRDESFDLLALRKESLPWQKKIILKIITEVNAEIAINENKLDELVRLVNNTKQAQATIDIGKNYKFEITYNYLRIGKK
ITVKGEQVKKRVVTLNSWLNLDGDAQFQLSNQKSALDADETMVLSANQLRFPLFIRKINPNDRLRLKNGGYKTIRRELID
QKVPNEQRERYFAITDVDDEILWVPGLRKAWLKEPFLKDNQQFIMEFRIGGSPHGQ
>Mature_456_residues
MKIGELGDQIVEQYGLSQFKIVVAVSTGVDSTVLINALINSTKIKHQQLIVAHVNHQLREQSIEEERFLHKWCRQHQITF
METDWPKSQHPQHGVEAAAREFRYTFFKQVMHDQHAQVLLTAHNADEQAENFVMQVIRGGWLNQLNGIPERRTFAQGWLI
RPFLKFTKQFLIETAQENKLRWFEDQTNRTTEFLRNRVRNEIIPAFRKENPRVVEHIQHYQDQLLEQSNLVDQVVLEKLT
KLQRDESFDLLALRKESLPWQKKIILKIITEVNAEIAINENKLDELVRLVNNTKQAQATIDIGKNYKFEITYNYLRIGKK
ITVKGEQVKKRVVTLNSWLNLDGDAQFQLSNQKSALDADETMVLSANQLRFPLFIRKINPNDRLRLKNGGYKTIRRELID
QKVPNEQRERYFAITDVDDEILWVPGLRKAWLKEPFLKDNQQFIMEFRIGGSPHGQ

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=217, Percent_Identity=30.8755760368664, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 53609; Mature: 53609

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGELGDQIVEQYGLSQFKIVVAVSTGVDSTVLINALINSTKIKHQQLIVAHVNHQLRE
CCCHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSIEEERFLHKWCRQHQITFMETDWPKSQHPQHGVEAAAREFRYTFFKQVMHDQHAQVLL
HHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TAHNADEQAENFVMQVIRGGWLNQLNGIPERRTFAQGWLIRPFLKFTKQFLIETAQENKL
EECCCCHHHHHHHHHHHHCCHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
RWFEDQTNRTTEFLRNRVRNEIIPAFRKENPRVVEHIQHYQDQLLEQSNLVDQVVLEKLT
EEHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLQRDESFDLLALRKESLPWQKKIILKIITEVNAEIAINENKLDELVRLVNNTKQAQATI
HHHCCCCCCEEEEHHHCCCHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCHHHEEEE
DIGKNYKFEITYNYLRIGKKITVKGEQVKKRVVTLNSWLNLDGDAQFQLSNQKSALDADE
EECCCEEEEEEEEEECCCCEEEECHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCC
TMVLSANQLRFPLFIRKINPNDRLRLKNGGYKTIRRELIDQKVPNEQRERYFAITDVDDE
EEEEECCCCEEEEEEEEECCCCCEEECCCCHHHHHHHHHHHCCCCCHHHCEEEEECCCCC
ILWVPGLRKAWLKEPFLKDNQQFIMEFRIGGSPHGQ
EEECCCCHHHHHCCCCCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MKIGELGDQIVEQYGLSQFKIVVAVSTGVDSTVLINALINSTKIKHQQLIVAHVNHQLRE
CCCHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSIEEERFLHKWCRQHQITFMETDWPKSQHPQHGVEAAAREFRYTFFKQVMHDQHAQVLL
HHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TAHNADEQAENFVMQVIRGGWLNQLNGIPERRTFAQGWLIRPFLKFTKQFLIETAQENKL
EECCCCHHHHHHHHHHHHCCHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
RWFEDQTNRTTEFLRNRVRNEIIPAFRKENPRVVEHIQHYQDQLLEQSNLVDQVVLEKLT
EEHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLQRDESFDLLALRKESLPWQKKIILKIITEVNAEIAINENKLDELVRLVNNTKQAQATI
HHHCCCCCCEEEEHHHCCCHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCHHHEEEE
DIGKNYKFEITYNYLRIGKKITVKGEQVKKRVVTLNSWLNLDGDAQFQLSNQKSALDADE
EECCCEEEEEEEEEECCCCEEEECHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCC
TMVLSANQLRFPLFIRKINPNDRLRLKNGGYKTIRRELIDQKVPNEQRERYFAITDVDDE
EEEEECCCCEEEEEEEEECCCCCEEECCCCHHHHHHHHHHHCCCCCHHHCEEEEECCCCC
ILWVPGLRKAWLKEPFLKDNQQFIMEFRIGGSPHGQ
EEECCCCHHHHHCCCCCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]