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The map label for this gene is tmk

Identifier: 116493219

GI number: 116493219

Start: 1452449

End: 1453078

Strand: Reverse

Name: tmk

Synonym: PEPE_1481

Alternate gene names: 116493219

Gene position: 1453078-1452449 (Counterclockwise)

Preceding gene: 116493220

Following gene: 116493218

Centisome position: 79.3

GC content: 40.32

Gene sequence:

>630_bases
GTGAACGGGCATTTTATATCATTTGAAGGTCCAGATGGGGCTGGAAAAACAACGGTTCTGCAAGCGATGGTCGAACATTA
CCAACAGCGTTTGAAGGACCAATTAACAGTAACGAGGGAGCCCGGCGGAAATCCTATTTCAGAGGCAATTCGAACTATTA
TTTTGGATAAAAACAACACCGAGATGGATGAGCGAACGGAGGCCTTACTCTATGCGGCTGCTAGACGACAACATTTGGTG
GAAACGATTTTACCAGCTTTGAAAAAAAATCATGTGGTTTTTTGTGACCGATTTGTTGATAGCTCAATTGCATATCAAGG
AGCAGGCAGAAAAATTGGTACCGATGCCGTCTATCAAATGAATCTCTTTGCTACTGAAGGAGCATTACCAGAAAAAACAA
TTTACTTGGATGTACCATCAGAACTTGGACTAAAGCGCATTATGACTCATCGGACGGAAGATGTTGATCGGTTAGATTTA
GAACAATTAGATTTTCATCAGCGAGTCCGTTCGGCATATCTAGATTTAGCTAAGAAATTTCCAGATCGGATTGTCGTAAT
TGATGCGAGTCAAGAACTTGATGATGTAAAGAGAGATGTAATTGAAGTGTTAGACCAAATTTTAAATTAG

Upstream 100 bases:

>100_bases
AATATGGTGATATTTTAATTAAGGTTAATAGACAGTCCTTAAAATCTAAAGTAGAATAAAAACAATAAATAATTTAAAAC
AAGTAAGGCGGGGAATACTG

Downstream 100 bases:

>100_bases
CGGAGGAATATTATTATGGCAACTAAGTTAGTTATCGCAATTGTACAAGATAAAGATGCAAACTATTTGAGTGATCAGTT
TATTGATCAAAATGTTCGAG

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MNGHFISFEGPDGAGKTTVLQAMVEHYQQRLKDQLTVTREPGGNPISEAIRTIILDKNNTEMDERTEALLYAAARRQHLV
ETILPALKKNHVVFCDRFVDSSIAYQGAGRKIGTDAVYQMNLFATEGALPEKTIYLDVPSELGLKRIMTHRTEDVDRLDL
EQLDFHQRVRSAYLDLAKKFPDRIVVIDASQELDDVKRDVIEVLDQILN

Sequences:

>Translated_209_residues
MNGHFISFEGPDGAGKTTVLQAMVEHYQQRLKDQLTVTREPGGNPISEAIRTIILDKNNTEMDERTEALLYAAARRQHLV
ETILPALKKNHVVFCDRFVDSSIAYQGAGRKIGTDAVYQMNLFATEGALPEKTIYLDVPSELGLKRIMTHRTEDVDRLDL
EQLDFHQRVRSAYLDLAKKFPDRIVVIDASQELDDVKRDVIEVLDQILN
>Mature_209_residues
MNGHFISFEGPDGAGKTTVLQAMVEHYQQRLKDQLTVTREPGGNPISEAIRTIILDKNNTEMDERTEALLYAAARRQHLV
ETILPALKKNHVVFCDRFVDSSIAYQGAGRKIGTDAVYQMNLFATEGALPEKTIYLDVPSELGLKRIMTHRTEDVDRLDL
EQLDFHQRVRSAYLDLAKKFPDRIVVIDASQELDDVKRDVIEVLDQILN

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=200, Percent_Identity=38, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_PEDPA (Q03E60)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804954.1
- ProteinModelPortal:   Q03E60
- SMR:   Q03E60
- STRING:   Q03E60
- GeneID:   4417251
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1481
- NMPDR:   fig|278197.10.peg.1305
- eggNOG:   COG0125
- HOGENOM:   HBG626009
- OMA:   LFITLEG
- PhylomeDB:   Q03E60
- BioCyc:   PPEN278197:PEPE_1481-MONOMER
- HAMAP:   MF_00165
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 23722; Mature: 23722

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGHFISFEGPDGAGKTTVLQAMVEHYQQRLKDQLTVTREPGGNPISEAIRTIILDKNNT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCC
EMDERTEALLYAAARRQHLVETILPALKKNHVVFCDRFVDSSIAYQGAGRKIGTDAVYQM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCHHHCCCCCCCCCCCCEEEE
NLFATEGALPEKTIYLDVPSELGLKRIMTHRTEDVDRLDLEQLDFHQRVRSAYLDLAKKF
EEEEECCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
PDRIVVIDASQELDDVKRDVIEVLDQILN
CCEEEEEECCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNGHFISFEGPDGAGKTTVLQAMVEHYQQRLKDQLTVTREPGGNPISEAIRTIILDKNNT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCC
EMDERTEALLYAAARRQHLVETILPALKKNHVVFCDRFVDSSIAYQGAGRKIGTDAVYQM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCHHHCCCCCCCCCCCCEEEE
NLFATEGALPEKTIYLDVPSELGLKRIMTHRTEDVDRLDLEQLDFHQRVRSAYLDLAKKF
EEEEECCCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
PDRIVVIDASQELDDVKRDVIEVLDQILN
CCEEEEEECCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA