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The map label for this gene is purK [H]

Identifier: 116493204

GI number: 116493204

Start: 1440116

End: 1441255

Strand: Reverse

Name: purK [H]

Synonym: PEPE_1466

Alternate gene names: 116493204

Gene position: 1441255-1440116 (Counterclockwise)

Preceding gene: 116493205

Following gene: 116493203

Centisome position: 78.65

GC content: 40.0

Gene sequence:

>1140_bases
GTGAAAAGCAATTCTGAAAAAGGGATTATTTTACCACCGGCAACAATTGGGATTATTGGTGGTGGACAGTTAGGTCAAAT
GATGGCGTTAGCGGCTAAAGCTATGGGATATAAGGTGGGGGTTCTAGATCCTACGCGAGATTGCCCAACCAGCCAAGTTT
GTGATTTTCAGATTGTAGCCGAATATACTGACCATCACGCCTTAATGCAAATGGCGGAGCAGAGCGATGTTTTAACTTAT
GAATTTGAGAATGTTGATTTAGCTAGTTTGAAAGTAGCACAAGAATTGGTAACCATTCCGCAGGGAACCAACTTACTAGC
TATTACTGGAGACCGAATCCGAGAAAAAAATTTTTTGAGTAGTCATGGAATTCCGGTTACCTCCTATCAAGTTGTTGAAA
AGCCTACTGATTTAGAAATGGCAATTGCTAAATTAGGTTATCCAAGCATTTTGAAAACTAGTGAAGGAGGTTACGACGGA
CATGGACAACAAGATATTAATTCCGAAGCGGATTTACTTGCTGGACAACGTTTAGTAGCCCAAGCTCCATGTATATTGGA
ACAACGTCAAAATTTTACTAAAGAATTATCTGTCATGGTAACGCGCAGCCGCGAAGGAATCGTCCAATGTTTTCCAGTAG
CCGAGAATGAACATCAGCACCACATTCTACATCAAAGCATTGTACCAGCCCGGGTTTCTCAAAACGTACAAGATAATGCT
CGAAAAATTGCTCAAAAAATTGCTCAGAAATTAGAACTAAGGGGCGTTTTAGGAATCGAATTTTTTATGCTAGAAAATGG
CCAATTATTGGTTAATGAAATGGCACCAAGGCCACATAATTCGGGACATTATTCCATCGAAGCATGTAATATTTCGCAGT
TCGAAGCCCACATTCGGAGTATTTGTGGATTGGCTTTACCAAAAGTCACACAAACTACACCAGCGGTGATGGTAAATCTT
CTCGGGCAGCACCTTGAAGCTGCTCGTCAGAAGTTAGTTCAAAGACCCGATTGGCATTTTCATGACTATGGGAAAGTCGA
AAGTCGAGTGAATCGAAAAATGGGACACATCACAATATTAGGTGATATTCAAAAAAGCTTAGATTCAATTAAATCAGAAA
ACATTTGGGAGATAAAATAA

Upstream 100 bases:

>100_bases
GCCGCTTTATTAGCAGCTGAAATTTTAGCGTTAACCGATAAAAAAGTTGCTGAAAAATTAATTGAATTCCGTCAAAAACA
GACTCAGGAGGCTATTGAGA

Downstream 100 bases:

>100_bases
TGAAGAAGACCACGTTGATTAAAGAAGGCAAAGCAAAAAGACTTTACAATACAGATGAAAAGGATTTGGTGTGGGTTGAA
TATATGGATCAAGCAACTGC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKSNSEKGIILPPATIGIIGGGQLGQMMALAAKAMGYKVGVLDPTRDCPTSQVCDFQIVAEYTDHHALMQMAEQSDVLTY
EFENVDLASLKVAQELVTIPQGTNLLAITGDRIREKNFLSSHGIPVTSYQVVEKPTDLEMAIAKLGYPSILKTSEGGYDG
HGQQDINSEADLLAGQRLVAQAPCILEQRQNFTKELSVMVTRSREGIVQCFPVAENEHQHHILHQSIVPARVSQNVQDNA
RKIAQKIAQKLELRGVLGIEFFMLENGQLLVNEMAPRPHNSGHYSIEACNISQFEAHIRSICGLALPKVTQTTPAVMVNL
LGQHLEAARQKLVQRPDWHFHDYGKVESRVNRKMGHITILGDIQKSLDSIKSENIWEIK

Sequences:

>Translated_379_residues
MKSNSEKGIILPPATIGIIGGGQLGQMMALAAKAMGYKVGVLDPTRDCPTSQVCDFQIVAEYTDHHALMQMAEQSDVLTY
EFENVDLASLKVAQELVTIPQGTNLLAITGDRIREKNFLSSHGIPVTSYQVVEKPTDLEMAIAKLGYPSILKTSEGGYDG
HGQQDINSEADLLAGQRLVAQAPCILEQRQNFTKELSVMVTRSREGIVQCFPVAENEHQHHILHQSIVPARVSQNVQDNA
RKIAQKIAQKLELRGVLGIEFFMLENGQLLVNEMAPRPHNSGHYSIEACNISQFEAHIRSICGLALPKVTQTTPAVMVNL
LGQHLEAARQKLVQRPDWHFHDYGKVESRVNRKMGHITILGDIQKSLDSIKSENIWEIK
>Mature_379_residues
MKSNSEKGIILPPATIGIIGGGQLGQMMALAAKAMGYKVGVLDPTRDCPTSQVCDFQIVAEYTDHHALMQMAEQSDVLTY
EFENVDLASLKVAQELVTIPQGTNLLAITGDRIREKNFLSSHGIPVTSYQVVEKPTDLEMAIAKLGYPSILKTSEGGYDG
HGQQDINSEADLLAGQRLVAQAPCILEQRQNFTKELSVMVTRSREGIVQCFPVAENEHQHHILHQSIVPARVSQNVQDNA
RKIAQKIAQKLELRGVLGIEFFMLENGQLLVNEMAPRPHNSGHYSIEACNISQFEAHIRSICGLALPKVTQTTPAVMVNL
LGQHLEAARQKLVQRPDWHFHDYGKVESRVNRKMGHITILGDIQKSLDSIKSENIWEIK

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=347, Percent_Identity=31.4121037463977, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1788155, Length=380, Percent_Identity=23.1578947368421, Blast_Score=96, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324702, Length=365, Percent_Identity=36.986301369863, Blast_Score=223, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 41983; Mature: 41983

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSNSEKGIILPPATIGIIGGGQLGQMMALAAKAMGYKVGVLDPTRDCPTSQVCDFQIVA
CCCCCCCCEEECCHHEEEECCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCEEEEE
EYTDHHALMQMAEQSDVLTYEFENVDLASLKVAQELVTIPQGTNLLAITGDRIREKNFLS
HHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHH
SHGIPVTSYQVVEKPTDLEMAIAKLGYPSILKTSEGGYDGHGQQDINSEADLLAGQRLVA
HCCCCCCCHHHHCCCCCHHHHHHHCCCCHHEECCCCCCCCCCCCCCCCHHHHHHCCHHHH
QAPCILEQRQNFTKELSVMVTRSREGIVQCFPVAENEHQHHILHQSIVPARVSQNVQDNA
HCCHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCHHHHHHHHHCHHHHHCCHHHHH
RKIAQKIAQKLELRGVLGIEFFMLENGQLLVNEMAPRPHNSGHYSIEACNISQFEAHIRS
HHHHHHHHHHHHHCCEEEEEEEEEECCCEEEECCCCCCCCCCCEEEEECCHHHHHHHHHH
ICGLALPKVTQTTPAVMVNLLGQHLEAARQKLVQRPDWHFHDYGKVESRVNRKMGHITIL
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEE
GDIQKSLDSIKSENIWEIK
HHHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MKSNSEKGIILPPATIGIIGGGQLGQMMALAAKAMGYKVGVLDPTRDCPTSQVCDFQIVA
CCCCCCCCEEECCHHEEEECCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCEEEEE
EYTDHHALMQMAEQSDVLTYEFENVDLASLKVAQELVTIPQGTNLLAITGDRIREKNFLS
HHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHH
SHGIPVTSYQVVEKPTDLEMAIAKLGYPSILKTSEGGYDGHGQQDINSEADLLAGQRLVA
HCCCCCCCHHHHCCCCCHHHHHHHCCCCHHEECCCCCCCCCCCCCCCCHHHHHHCCHHHH
QAPCILEQRQNFTKELSVMVTRSREGIVQCFPVAENEHQHHILHQSIVPARVSQNVQDNA
HCCHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCHHHHHHHHHCHHHHHCCHHHHH
RKIAQKIAQKLELRGVLGIEFFMLENGQLLVNEMAPRPHNSGHYSIEACNISQFEAHIRS
HHHHHHHHHHHHHCCEEEEEEEEEECCCEEEECCCCCCCCCCCEEEEECCHHHHHHHHHH
ICGLALPKVTQTTPAVMVNLLGQHLEAARQKLVQRPDWHFHDYGKVESRVNRKMGHITIL
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEE
GDIQKSLDSIKSENIWEIK
HHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]