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The map label for this gene is purD [H]

Identifier: 116493195

GI number: 116493195

Start: 1430335

End: 1431594

Strand: Reverse

Name: purD [H]

Synonym: PEPE_1457

Alternate gene names: 116493195

Gene position: 1431594-1430335 (Counterclockwise)

Preceding gene: 116493196

Following gene: 116493194

Centisome position: 78.13

GC content: 40.79

Gene sequence:

>1260_bases
ATGGCAAAAGTACTTGTGGTTGGAAATGGTGCAAGGGAGCACGTTCTTGGACGTCAGCTATTGAAAAGTAATCAAGTAGA
AAAAGTTTACTGTGCACCCGGAAATCCAGGGATGTTGGAAAGTGGCATTGAAATTATTCCTATTGGCGTCACAGATTTTG
GGAAATTAGTTGAGTTCGCCCGGAAACAAGGTATTGATTTGACGGTTGTCGGACCAGAACAACCTCTACAGCAAGGAATT
GTAGATTATTTTAGTACTGCGGGGTTAACGATTTTTGGACCGACTCAAGCAGCCGCTAAACTAGAAAGTTCTAAAGAATT
TGCTAAAAAGATTATGCAAGAAGCGGGGGTCCCCACGGCTAGTTATGTTAGCTATCAAGATGAAGCTACCGCAATCAAAG
GGCTAGCGAAAAATGATTATCCACTGGTAATTAAATCGGATGGCTTAGCGGCTGGAAAAGGAGTCGTTATTGCTAAAAGT
CAGAAGGAAGCTACGACAACGATTCATAAAATGTTGGGTGAACATCAATTTGATACTAAGTCTATTATTATCGAAGAATT
TTTAACAGGACAGGAATTTTCCTTGATGGCTTTTATTCAAGACGAACAAATTATTCCCATGCCTCTATCGCAAGATCACA
AGGCAGCTTATGATGGTGACGAAGGCCCCAATACAGGTGGGATGGGAGCGTATAGTCCCTTGCAGCATTTTCCAAGTGAT
TTGGTTAATGAAGGTGTGGAAAAAATTATTCGTCCTATTATTCGAACACTTAAAAAGAAGAATATTGCTTTTTGTGGGAT
TCTATACGCTGGTCTAATAATGACAGACTCCGGTCCTAAAGTGATTGAATTTAATGTACGGTTGGGTGATCCTGAAACGG
TGGTAGTATTACCGCAGCTACAAAGTGATTTATACGAAATGTTGCAGGGATTGCTCCACCACCAAGTGCCAGTGGTTAAA
TGGCAAAAAGACCAGGTTTACTTAGGAGTAGTAATTAGTTCGCAAACTTATCCGCAAGCTTCGGCAAGGGGAGTTGAACT
AGCAGATTTTAATTACCTTCCATCCAATATTACAGTTAATTATGCGGGCGTTAGTTGCAAGCGAGACGGCCAATTAATTA
GTAATGGGGGACGAATCCTATGTTTGACCACTGGAGCGAGCAATATTACGAAAGCTCAAGCAATATTGTACGGATGGTTA
AATCAGCATCCAATTCTGGGCTTACGTTATCGAAGTGATATTGGAGATAAAGCAAAATAA

Upstream 100 bases:

>100_bases
AGTATCAAAGATCAAGCTTCAATTGATAAGGCGGATGAGTTAGGAATTTCATTAATATTTAGTGGACGTCGTCACTTTAG
ACATTAAGCGGAGGTACCAG

Downstream 100 bases:

>100_bases
AAAATAACCATGGAAGAACGATGACGGCGACTTAAAATAATTCGATTCCATTGTTTTTTTAACTAAAAAATTAGTAACAT
TTAATACTATATAAAGTGTA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MAKVLVVGNGAREHVLGRQLLKSNQVEKVYCAPGNPGMLESGIEIIPIGVTDFGKLVEFARKQGIDLTVVGPEQPLQQGI
VDYFSTAGLTIFGPTQAAAKLESSKEFAKKIMQEAGVPTASYVSYQDEATAIKGLAKNDYPLVIKSDGLAAGKGVVIAKS
QKEATTTIHKMLGEHQFDTKSIIIEEFLTGQEFSLMAFIQDEQIIPMPLSQDHKAAYDGDEGPNTGGMGAYSPLQHFPSD
LVNEGVEKIIRPIIRTLKKKNIAFCGILYAGLIMTDSGPKVIEFNVRLGDPETVVVLPQLQSDLYEMLQGLLHHQVPVVK
WQKDQVYLGVVISSQTYPQASARGVELADFNYLPSNITVNYAGVSCKRDGQLISNGGRILCLTTGASNITKAQAILYGWL
NQHPILGLRYRSDIGDKAK

Sequences:

>Translated_419_residues
MAKVLVVGNGAREHVLGRQLLKSNQVEKVYCAPGNPGMLESGIEIIPIGVTDFGKLVEFARKQGIDLTVVGPEQPLQQGI
VDYFSTAGLTIFGPTQAAAKLESSKEFAKKIMQEAGVPTASYVSYQDEATAIKGLAKNDYPLVIKSDGLAAGKGVVIAKS
QKEATTTIHKMLGEHQFDTKSIIIEEFLTGQEFSLMAFIQDEQIIPMPLSQDHKAAYDGDEGPNTGGMGAYSPLQHFPSD
LVNEGVEKIIRPIIRTLKKKNIAFCGILYAGLIMTDSGPKVIEFNVRLGDPETVVVLPQLQSDLYEMLQGLLHHQVPVVK
WQKDQVYLGVVISSQTYPQASARGVELADFNYLPSNITVNYAGVSCKRDGQLISNGGRILCLTTGASNITKAQAILYGWL
NQHPILGLRYRSDIGDKAK
>Mature_418_residues
AKVLVVGNGAREHVLGRQLLKSNQVEKVYCAPGNPGMLESGIEIIPIGVTDFGKLVEFARKQGIDLTVVGPEQPLQQGIV
DYFSTAGLTIFGPTQAAAKLESSKEFAKKIMQEAGVPTASYVSYQDEATAIKGLAKNDYPLVIKSDGLAAGKGVVIAKSQ
KEATTTIHKMLGEHQFDTKSIIIEEFLTGQEFSLMAFIQDEQIIPMPLSQDHKAAYDGDEGPNTGGMGAYSPLQHFPSDL
VNEGVEKIIRPIIRTLKKKNIAFCGILYAGLIMTDSGPKVIEFNVRLGDPETVVVLPQLQSDLYEMLQGLLHHQVPVVKW
QKDQVYLGVVISSQTYPQASARGVELADFNYLPSNITVNYAGVSCKRDGQLISNGGRILCLTTGASNITKAQAILYGWLN
QHPILGLRYRSDIGDKAK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=40.3755868544601, Blast_Score=316, Evalue=2e-86,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=40.3755868544601, Blast_Score=316, Evalue=2e-86,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=40.3755868544601, Blast_Score=316, Evalue=2e-86,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=40.3755868544601, Blast_Score=316, Evalue=2e-86,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=41.3145539906103, Blast_Score=312, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17567511, Length=420, Percent_Identity=35.7142857142857, Blast_Score=252, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=37.0454545454545, Blast_Score=262, Evalue=8e-71,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=35.0348027842227, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24582400, Length=438, Percent_Identity=35.3881278538813, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45505; Mature: 45374

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVLVVGNGAREHVLGRQLLKSNQVEKVYCAPGNPGMLESGIEIIPIGVTDFGKLVEFA
CEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHCCCEEEEECCCCHHHHHHHH
RKQGIDLTVVGPEQPLQQGIVDYFSTAGLTIFGPTQAAAKLESSKEFAKKIMQEAGVPTA
HHCCCEEEEECCCHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCH
SYVSYQDEATAIKGLAKNDYPLVIKSDGLAAGKGVVIAKSQKEATTTIHKMLGEHQFDTK
HHEECCCHHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCHH
SIIIEEFLTGQEFSLMAFIQDEQIIPMPLSQDHKAAYDGDEGPNTGGMGAYSPLQHFPSD
HHHHHHHHCCCCEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCHHH
LVNEGVEKIIRPIIRTLKKKNIAFCGILYAGLIMTDSGPKVIEFNVRLGDPETVVVLPQL
HHHHHHHHHHHHHHHHHHHCCCEEHHHHHCCEEEECCCCEEEEEEEEECCCCEEEECCHH
QSDLYEMLQGLLHHQVPVVKWQKDQVYLGVVISSQTYPQASARGVELADFNYLPSNITVN
HHHHHHHHHHHHHHCCCEEEEECCEEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCEEE
YAGVSCKRDGQLISNGGRILCLTTGASNITKAQAILYGWLNQHPILGLRYRSDIGDKAK
EECEEECCCCCEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
AKVLVVGNGAREHVLGRQLLKSNQVEKVYCAPGNPGMLESGIEIIPIGVTDFGKLVEFA
EEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHCCCEEEEECCCCHHHHHHHH
RKQGIDLTVVGPEQPLQQGIVDYFSTAGLTIFGPTQAAAKLESSKEFAKKIMQEAGVPTA
HHCCCEEEEECCCHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCH
SYVSYQDEATAIKGLAKNDYPLVIKSDGLAAGKGVVIAKSQKEATTTIHKMLGEHQFDTK
HHEECCCHHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCHH
SIIIEEFLTGQEFSLMAFIQDEQIIPMPLSQDHKAAYDGDEGPNTGGMGAYSPLQHFPSD
HHHHHHHHCCCCEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCHHH
LVNEGVEKIIRPIIRTLKKKNIAFCGILYAGLIMTDSGPKVIEFNVRLGDPETVVVLPQL
HHHHHHHHHHHHHHHHHHHCCCEEHHHHHCCEEEECCCCEEEEEEEEECCCCEEEECCHH
QSDLYEMLQGLLHHQVPVVKWQKDQVYLGVVISSQTYPQASARGVELADFNYLPSNITVN
HHHHHHHHHHHHHHCCCEEEEECCEEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCEEE
YAGVSCKRDGQLISNGGRILCLTTGASNITKAQAILYGWLNQHPILGLRYRSDIGDKAK
EECEEECCCCCEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]