Click here to switch to the map view.

The map label for this gene is clpB [H]

Identifier: 116493171

GI number: 116493171

Start: 1406138

End: 1408603

Strand: Reverse

Name: clpB [H]

Synonym: PEPE_1427

Alternate gene names: 116493171

Gene position: 1408603-1406138 (Counterclockwise)

Preceding gene: 116493172

Following gene: 116493170

Centisome position: 76.87

GC content: 39.74

Gene sequence:

>2466_bases
ATGGAAAATTTATTTACCCCTAGTGCAAGAAATGCCTTGGTTATTGCTCAAGAACAAGCAAAAAGATTTAAACACCCCGC
GGTGGGTACTGAACACCTGTTGTTAGCATTGGCAATTGAGACCAATGGTGTAGCGCGAAGCGTCTTAGGACAATTCAATG
TTACAGAAGTGGACGTTGCTGAGGAAATTGAGCAATTTACCGGCTATGGTAACTTACGAACAGTAAAAGATGATTATTTA
CCATATTCACCAAAAGCTAGTGAAATTTTAGTCAACGCTCAAGAGCAGGCTAAGCAATTAAACGCTATTAAAATTGGAAC
CGAACATTTATTACTTGCTTTAGTACAAAATGATAATATTTTGTCTTCAAGAATTTTATTGGCGTTGGATACAGATATTG
CCGAGGTTCGCAAAGTTACTTTACGTAAATTAGGTGTAAATCCAGCTACTCAAAATCGACGTAGTAAGCAACGGGCTACA
AGTGCTGAAGGAACCCCAACTTTAGATTCAGTTGCACGAGACTTGACGGCGATGGCTGCTGAAGGAAAAATTGATCCAGT
GGTGGGGCGGACAAAAGAAGTTCGTCGCGTAATCCAAATTTTAAGCCGACGGACTAAGAACAATCCAGTATTGATTGGTG
AACCAGGAGTTGGGAAGACGGCGATTGCCGAAGGATTAGCTCAAAAGATTATCGATCAAGATGTTCCAACTGATATGCAA
AATAAACGATTAATGATGCTAGAGATGGGTTCTTTAGTGGCGGGAACTAAGTACCGTGGTGAATTTGAAGATCGTTTAAA
AAAGGTGATTGAAGAAATTCGCAATGATGGTCAAGTGATTGTCTTTATCGATGAGTTACACACGTTAATTGGAGCTGGCG
GGGCTGAGGGAGCCATTGACGCTTCTAATATCCTAAAACCAGCTTTAGCTCGTGGCGAGCTACAAACGATTGGTGCAACT
ACTTTAACAGAATACCAAAAGTATATTGAAGCTGATGCTGCTTTGGAACGACGTTTTGCTAAAGTGGAAGTTGATGAACC
TACGGAAGCTGAAGCAGTTCAAATTTTAAGAGGAATTCGTCCTAAGTATGAAGAACATCATCAAGTTAAAATTTCAGATG
ATGCCATTCAACAAGCAGTTACTTTATCATCACGTTATATTGCAGACCGTTTTCTTCCCGATAAGGCCATCGATTTAATC
GATGAAGCGGCTGCTAAAATTCGAATCGATGCCTCTGAGAAGCAAGTGAAAAAAGTTACGGATGAAGATCGATTAGAAAA
ATTACGAACAGCTAAAGAAGAAGCGATTGATAATCAAGATTTCGAAAAGGCTGCCGACATCCGTAAGAAAGAAATGAAGT
TACGCCGTAAGATTGAGCGTGCTGAAAATAACCCAGAAACCAATGAAGCTAAAAAAGATTATAAATTAATTGAAAATGGC
GAAGATATTGCGCAAATCGTTTCAGATTGGACTGGTGTACCTTTAACGCAGATGAAACAATCTGAGTCAGATCGCTTGAT
TAATTTAGAGAAAACTTTGCACGAACGAGTAATTGGTCAAAAAGAAGCCGTTTCGTCAGTAGCTCGAGCTATTCGCCGTG
CGCGTTCAGGTTTGAAGAGCCCTAAGCGACCAATTGGTTCTTTTATGTTCCTTGGTCCTACAGGGGTTGGTAAAACAGAA
CTGGCTAAGTCTTTAGCAGATATTATGTTTGGTTCGGAAGATAATATGATCAGAATTGATATGTCTGAGTATATGGAACG
TTACAGCACTAGTCGTCTAGTTGGATCAGCACCAGGTTATGTGGGTTACGACGAGGGTGGACAATTGACTGAACAGGTTC
GTCGGAAACCATACTCAGTTGTTTTATTTGATGAAGTAGAAAAAGCTCATCCAGATGTCTTTAATTTGTTACTTCAAGTT
TTTGATGATGGTTTTCTAACTGATTCTAAGGGCCGACGAGTTGATTTTAGAAATACTATTATCATTATGACGTCTAACTT
GGGAGCTACAGCATTGAGGGATGAAAAATCAGTCGGCTTTGGTGCTCAAAATGCAGCTGAAGATTACCATGCTATGGCAG
ATAAAGTACGTGAAGTTTTGAAGCAGAGTTTCCGTCCCGAATTCCTCAACCGGATTGACGAAACCATTATTTTCCATTCA
TTAACGAAACCTGAACTCCATCAAATCGTTAAATTGATGGCTAAAGATGTCATTCAGCGGATGAGTGAGCAGGGAATTAA
TGCCAAGTTTACTCCTAGTGCAATTGATGTGGTGGCTGAAGCTGGATTTGATCCTGAATATGGGGCACGTCCATTGCGTC
GAGCCTTACAAACTAAGGTTGAAGACCAATTAAGTGAATTAATGTTGTCCGGTGAAGCTAAGGTTGGTGACGATATTACG
ATCGGAGCGCGCAACGGAAAAATTAATTTAAAAGTTAAAAATAAAACATTAATAAAAAATAAATAA

Upstream 100 bases:

>100_bases
CGATCGTAATTTAGAAGAACAAATTAGAGCAAAAGTGATGATTGGAATTTTGAATCATCTCAGATATGAATAGTAGAGAT
TAGAGAAAGACAGGTGGAAA

Downstream 100 bases:

>100_bases
TTTAACATTGCAAGTTGTTTGACAAAGCATATGGCAAGGAATAAAATGTAATATGTATGTTAAAGCAGGGTTAAATGAGA
CAGTGCGATCTATTGTCCGC

Product: ATP-binding subunit of Clp protease and DnaK/DnaJ chaperones

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 821; Mature: 821

Protein sequence:

>821_residues
MENLFTPSARNALVIAQEQAKRFKHPAVGTEHLLLALAIETNGVARSVLGQFNVTEVDVAEEIEQFTGYGNLRTVKDDYL
PYSPKASEILVNAQEQAKQLNAIKIGTEHLLLALVQNDNILSSRILLALDTDIAEVRKVTLRKLGVNPATQNRRSKQRAT
SAEGTPTLDSVARDLTAMAAEGKIDPVVGRTKEVRRVIQILSRRTKNNPVLIGEPGVGKTAIAEGLAQKIIDQDVPTDMQ
NKRLMMLEMGSLVAGTKYRGEFEDRLKKVIEEIRNDGQVIVFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGAT
TLTEYQKYIEADAALERRFAKVEVDEPTEAEAVQILRGIRPKYEEHHQVKISDDAIQQAVTLSSRYIADRFLPDKAIDLI
DEAAAKIRIDASEKQVKKVTDEDRLEKLRTAKEEAIDNQDFEKAADIRKKEMKLRRKIERAENNPETNEAKKDYKLIENG
EDIAQIVSDWTGVPLTQMKQSESDRLINLEKTLHERVIGQKEAVSSVARAIRRARSGLKSPKRPIGSFMFLGPTGVGKTE
LAKSLADIMFGSEDNMIRIDMSEYMERYSTSRLVGSAPGYVGYDEGGQLTEQVRRKPYSVVLFDEVEKAHPDVFNLLLQV
FDDGFLTDSKGRRVDFRNTIIIMTSNLGATALRDEKSVGFGAQNAAEDYHAMADKVREVLKQSFRPEFLNRIDETIIFHS
LTKPELHQIVKLMAKDVIQRMSEQGINAKFTPSAIDVVAEAGFDPEYGARPLRRALQTKVEDQLSELMLSGEAKVGDDIT
IGARNGKINLKVKNKTLIKNK

Sequences:

>Translated_821_residues
MENLFTPSARNALVIAQEQAKRFKHPAVGTEHLLLALAIETNGVARSVLGQFNVTEVDVAEEIEQFTGYGNLRTVKDDYL
PYSPKASEILVNAQEQAKQLNAIKIGTEHLLLALVQNDNILSSRILLALDTDIAEVRKVTLRKLGVNPATQNRRSKQRAT
SAEGTPTLDSVARDLTAMAAEGKIDPVVGRTKEVRRVIQILSRRTKNNPVLIGEPGVGKTAIAEGLAQKIIDQDVPTDMQ
NKRLMMLEMGSLVAGTKYRGEFEDRLKKVIEEIRNDGQVIVFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGAT
TLTEYQKYIEADAALERRFAKVEVDEPTEAEAVQILRGIRPKYEEHHQVKISDDAIQQAVTLSSRYIADRFLPDKAIDLI
DEAAAKIRIDASEKQVKKVTDEDRLEKLRTAKEEAIDNQDFEKAADIRKKEMKLRRKIERAENNPETNEAKKDYKLIENG
EDIAQIVSDWTGVPLTQMKQSESDRLINLEKTLHERVIGQKEAVSSVARAIRRARSGLKSPKRPIGSFMFLGPTGVGKTE
LAKSLADIMFGSEDNMIRIDMSEYMERYSTSRLVGSAPGYVGYDEGGQLTEQVRRKPYSVVLFDEVEKAHPDVFNLLLQV
FDDGFLTDSKGRRVDFRNTIIIMTSNLGATALRDEKSVGFGAQNAAEDYHAMADKVREVLKQSFRPEFLNRIDETIIFHS
LTKPELHQIVKLMAKDVIQRMSEQGINAKFTPSAIDVVAEAGFDPEYGARPLRRALQTKVEDQLSELMLSGEAKVGDDIT
IGARNGKINLKVKNKTLIKNK
>Mature_821_residues
MENLFTPSARNALVIAQEQAKRFKHPAVGTEHLLLALAIETNGVARSVLGQFNVTEVDVAEEIEQFTGYGNLRTVKDDYL
PYSPKASEILVNAQEQAKQLNAIKIGTEHLLLALVQNDNILSSRILLALDTDIAEVRKVTLRKLGVNPATQNRRSKQRAT
SAEGTPTLDSVARDLTAMAAEGKIDPVVGRTKEVRRVIQILSRRTKNNPVLIGEPGVGKTAIAEGLAQKIIDQDVPTDMQ
NKRLMMLEMGSLVAGTKYRGEFEDRLKKVIEEIRNDGQVIVFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGAT
TLTEYQKYIEADAALERRFAKVEVDEPTEAEAVQILRGIRPKYEEHHQVKISDDAIQQAVTLSSRYIADRFLPDKAIDLI
DEAAAKIRIDASEKQVKKVTDEDRLEKLRTAKEEAIDNQDFEKAADIRKKEMKLRRKIERAENNPETNEAKKDYKLIENG
EDIAQIVSDWTGVPLTQMKQSESDRLINLEKTLHERVIGQKEAVSSVARAIRRARSGLKSPKRPIGSFMFLGPTGVGKTE
LAKSLADIMFGSEDNMIRIDMSEYMERYSTSRLVGSAPGYVGYDEGGQLTEQVRRKPYSVVLFDEVEKAHPDVFNLLLQV
FDDGFLTDSKGRRVDFRNTIIIMTSNLGATALRDEKSVGFGAQNAAEDYHAMADKVREVLKQSFRPEFLNRIDETIIFHS
LTKPELHQIVKLMAKDVIQRMSEQGINAKFTPSAIDVVAEAGFDPEYGARPLRRALQTKVEDQLSELMLSGEAKVGDDIT
IGARNGKINLKVKNKTLIKNK

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=338, Percent_Identity=37.5739644970414, Blast_Score=203, Evalue=5e-52,
Organism=Escherichia coli, GI1788943, Length=684, Percent_Identity=50.2923976608187, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=807, Percent_Identity=36.6790582403965, Blast_Score=492, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6320464, Length=705, Percent_Identity=44.3971631205674, Blast_Score=589, Evalue=1e-169,
Organism=Saccharomyces cerevisiae, GI6323002, Length=881, Percent_Identity=37.4574347332577, Blast_Score=530, Evalue=1e-151,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- InterPro:   IPR001943 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small; PF02151 UVR [H]

EC number: NA

Molecular weight: Translated: 91431; Mature: 91431

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50151 UVR ; PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLFTPSARNALVIAQEQAKRFKHPAVGTEHLLLALAIETNGVARSVLGQFNVTEVDVA
CCCCCCCCCCCEEEEEHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHCCCCCCCHHHH
EEIEQFTGYGNLRTVKDDYLPYSPKASEILVNAQEQAKQLNAIKIGTEHLLLALVQNDNI
HHHHHHCCCCCCEECCCCCCCCCCCHHHHEECCHHHHHHHHHHEECHHHHEEEEECCCCC
LSSRILLALDTDIAEVRKVTLRKLGVNPATQNRRSKQRATSAEGTPTLDSVARDLTAMAA
HHCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
EGKIDPVVGRTKEVRRVIQILSRRTKNNPVLIGEPGVGKTAIAEGLAQKIIDQDVPTDMQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCC
NKRLMMLEMGSLVAGTKYRGEFEDRLKKVIEEIRNDGQVIVFIDELHTLIGAGGAEGAID
CCEEEEEECCCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCC
ASNILKPALARGELQTIGATTLTEYQKYIEADAALERRFAKVEVDEPTEAEAVQILRGIR
HHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCC
PKYEEHHQVKISDDAIQQAVTLSSRYIADRFLPDKAIDLIDEAAAKIRIDASEKQVKKVT
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEECCHHHHHHHCC
DEDRLEKLRTAKEEAIDNQDFEKAADIRKKEMKLRRKIERAENNPETNEAKKDYKLIENG
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
EDIAQIVSDWTGVPLTQMKQSESDRLINLEKTLHERVIGQKEAVSSVARAIRRARSGLKS
HHHHHHHHCCCCCCHHHHHCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
PKRPIGSFMFLGPTGVGKTELAKSLADIMFGSEDNMIRIDMSEYMERYSTSRLVGSAPGY
CCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCC
VGYDEGGQLTEQVRRKPYSVVLFDEVEKAHPDVFNLLLQVFDDGFLTDSKGRRVDFRNTI
CCCCCCCHHHHHHHHCCCEEEEEHHHHHHCHHHHHHHHHHHCCCCEECCCCCEEECCCEE
IIMTSNLGATALRDEKSVGFGAQNAAEDYHAMADKVREVLKQSFRPEFLNRIDETIIFHS
EEEECCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
LTKPELHQIVKLMAKDVIQRMSEQGINAKFTPSAIDVVAEAGFDPEYGARPLRRALQTKV
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
EDQLSELMLSGEAKVGDDITIGARNGKINLKVKNKTLIKNK
HHHHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MENLFTPSARNALVIAQEQAKRFKHPAVGTEHLLLALAIETNGVARSVLGQFNVTEVDVA
CCCCCCCCCCCEEEEEHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHCCCCCCCHHHH
EEIEQFTGYGNLRTVKDDYLPYSPKASEILVNAQEQAKQLNAIKIGTEHLLLALVQNDNI
HHHHHHCCCCCCEECCCCCCCCCCCHHHHEECCHHHHHHHHHHEECHHHHEEEEECCCCC
LSSRILLALDTDIAEVRKVTLRKLGVNPATQNRRSKQRATSAEGTPTLDSVARDLTAMAA
HHCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
EGKIDPVVGRTKEVRRVIQILSRRTKNNPVLIGEPGVGKTAIAEGLAQKIIDQDVPTDMQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCC
NKRLMMLEMGSLVAGTKYRGEFEDRLKKVIEEIRNDGQVIVFIDELHTLIGAGGAEGAID
CCEEEEEECCCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCC
ASNILKPALARGELQTIGATTLTEYQKYIEADAALERRFAKVEVDEPTEAEAVQILRGIR
HHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCC
PKYEEHHQVKISDDAIQQAVTLSSRYIADRFLPDKAIDLIDEAAAKIRIDASEKQVKKVT
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEECCHHHHHHHCC
DEDRLEKLRTAKEEAIDNQDFEKAADIRKKEMKLRRKIERAENNPETNEAKKDYKLIENG
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
EDIAQIVSDWTGVPLTQMKQSESDRLINLEKTLHERVIGQKEAVSSVARAIRRARSGLKS
HHHHHHHHCCCCCCHHHHHCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
PKRPIGSFMFLGPTGVGKTELAKSLADIMFGSEDNMIRIDMSEYMERYSTSRLVGSAPGY
CCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCC
VGYDEGGQLTEQVRRKPYSVVLFDEVEKAHPDVFNLLLQVFDDGFLTDSKGRRVDFRNTI
CCCCCCCHHHHHHHHCCCEEEEEHHHHHHCHHHHHHHHHHHCCCCEECCCCCEEECCCEE
IIMTSNLGATALRDEKSVGFGAQNAAEDYHAMADKVREVLKQSFRPEFLNRIDETIIFHS
EEEECCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
LTKPELHQIVKLMAKDVIQRMSEQGINAKFTPSAIDVVAEAGFDPEYGARPLRRALQTKV
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
EDQLSELMLSGEAKVGDDITIGARNGKINLKVKNKTLIKNK
HHHHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]