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The map label for this gene is ywkE [H]

Identifier: 116493077

GI number: 116493077

Start: 1318543

End: 1319394

Strand: Reverse

Name: ywkE [H]

Synonym: PEPE_1329

Alternate gene names: 116493077

Gene position: 1319394-1318543 (Counterclockwise)

Preceding gene: 116493078

Following gene: 116493076

Centisome position: 72.0

GC content: 39.79

Gene sequence:

>852_bases
ATGAATAATATTCCAACTTATTTTGAAGCCCTGAGATGGGCTTCTTTGTTTATCCGAAAAAACCAAGGAGACGAGAATGC
TCCAGAATTGATCTTGATGGATACAATGGAGTGGTCAAGAACCGAATTAATCATGCATTACCGTGAAAAACTTTTTCCCG
AACAGTGGGAGAAGTTTCAAACCGCTGTGAAAAGAGTTGCCAAGGGAGAGCCTGTGCAATACGTAACGAATAAGGCTACT
TTTTTTGGACGTGAATTTTACGTTGACAAACGGGTGTTAATTCCACGGGTTGAAACGGAAGAGTTAGTCGAAACGATTCT
AAGTAAAACTAAGCGCAGTCGACAGAGACTTCGAGTTTTAGATATTGGGACGGGGAGTGGTGATATAGCGATTACGCTTA
AATTGGAGCGTCCAGAATGGTTAGTCACGGCGGTCGATATCTCTAAAGATGCTTTAACAGTGGCTCAACGGAATGCTGAA
AGTCATGAAGCTATCGTTGATTTTAGGCTAGGGTCATTGTTTGAACCTGTCCAAGGGGAACGTTTTGATTTAATTATCAG
CAATCCGCCCTATATTGCGGATAATGAAAAGCATGAAATGGATCAATCAGTGATTGACTTTGAGCCTCATCAAGCCTTAT
TTGCTGACGACCACGGATTATTTTGGTATAAAAGAATTGCAGATCAATTGGATCAATATTTAGTCGAACACGGAGAATTG
GGTTGCGAAATTGGTTATCGTCAAGGTACGGACCTAAAAAAGTATTTCTTAGAAAAGAAGTATATTGACCAAGCGGAAGT
TATAAAAGATCTTAGTCAACACGATCGAATTCTTTGGGTTAAAAAGAAATAG

Upstream 100 bases:

>100_bases
ACTTTGAACAAGTTAGATCGAGTTATGAATGGTGAATTAGATGATATCATCGATGCTCTTATTCTCGATGATCAAGCCAA
GAAAGTGGAACAACTTCGTA

Downstream 100 bases:

>100_bases
GTGATAAAAATGAGTAAATTATTTAGTTCTGATGAAATACAAGTAGCGGCTCAAGCCATCAAAGCTGGTGAATTAGTATC
TTTCCCAACCGAAACCGTTT

Product: methylase of polypeptide chain release factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MNNIPTYFEALRWASLFIRKNQGDENAPELILMDTMEWSRTELIMHYREKLFPEQWEKFQTAVKRVAKGEPVQYVTNKAT
FFGREFYVDKRVLIPRVETEELVETILSKTKRSRQRLRVLDIGTGSGDIAITLKLERPEWLVTAVDISKDALTVAQRNAE
SHEAIVDFRLGSLFEPVQGERFDLIISNPPYIADNEKHEMDQSVIDFEPHQALFADDHGLFWYKRIADQLDQYLVEHGEL
GCEIGYRQGTDLKKYFLEKKYIDQAEVIKDLSQHDRILWVKKK

Sequences:

>Translated_283_residues
MNNIPTYFEALRWASLFIRKNQGDENAPELILMDTMEWSRTELIMHYREKLFPEQWEKFQTAVKRVAKGEPVQYVTNKAT
FFGREFYVDKRVLIPRVETEELVETILSKTKRSRQRLRVLDIGTGSGDIAITLKLERPEWLVTAVDISKDALTVAQRNAE
SHEAIVDFRLGSLFEPVQGERFDLIISNPPYIADNEKHEMDQSVIDFEPHQALFADDHGLFWYKRIADQLDQYLVEHGEL
GCEIGYRQGTDLKKYFLEKKYIDQAEVIKDLSQHDRILWVKKK
>Mature_283_residues
MNNIPTYFEALRWASLFIRKNQGDENAPELILMDTMEWSRTELIMHYREKLFPEQWEKFQTAVKRVAKGEPVQYVTNKAT
FFGREFYVDKRVLIPRVETEELVETILSKTKRSRQRLRVLDIGTGSGDIAITLKLERPEWLVTAVDISKDALTVAQRNAE
SHEAIVDFRLGSLFEPVQGERFDLIISNPPYIADNEKHEMDQSVIDFEPHQALFADDHGLFWYKRIADQLDQYLVEHGEL
GCEIGYRQGTDLKKYFLEKKYIDQAEVIKDLSQHDRILWVKKK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Escherichia coli, GI1787463, Length=269, Percent_Identity=33.457249070632, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI87082085, Length=186, Percent_Identity=39.247311827957, Blast_Score=120, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24582226, Length=244, Percent_Identity=25.8196721311475, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 33227; Mature: 33227

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIPTYFEALRWASLFIRKNQGDENAPELILMDTMEWSRTELIMHYREKLFPEQWEKFQ
CCCCCHHHHHHHHHHHHEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCHHHHHHHH
TAVKRVAKGEPVQYVTNKATFFGREFYVDKRVLIPRVETEELVETILSKTKRSRQRLRVL
HHHHHHHCCCCCEEEECCHHHCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHEEEE
DIGTGSGDIAITLKLERPEWLVTAVDISKDALTVAQRNAESHEAIVDFRLGSLFEPVQGE
EECCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHCCCCCHHHHEEEHHHHHCCCCCCC
RFDLIISNPPYIADNEKHEMDQSVIDFEPHQALFADDHGLFWYKRIADQLDQYLVEHGEL
EEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCC
GCEIGYRQGTDLKKYFLEKKYIDQAEVIKDLSQHDRILWVKKK
CEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure
MNNIPTYFEALRWASLFIRKNQGDENAPELILMDTMEWSRTELIMHYREKLFPEQWEKFQ
CCCCCHHHHHHHHHHHHEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCHHHHHHHH
TAVKRVAKGEPVQYVTNKATFFGREFYVDKRVLIPRVETEELVETILSKTKRSRQRLRVL
HHHHHHHCCCCCEEEECCHHHCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHEEEE
DIGTGSGDIAITLKLERPEWLVTAVDISKDALTVAQRNAESHEAIVDFRLGSLFEPVQGE
EECCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHCCCCCHHHHEEEHHHHHCCCCCCC
RFDLIISNPPYIADNEKHEMDQSVIDFEPHQALFADDHGLFWYKRIADQLDQYLVEHGEL
EEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCC
GCEIGYRQGTDLKKYFLEKKYIDQAEVIKDLSQHDRILWVKKK
CEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]