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The map label for this gene is prfA [H]

Identifier: 116493078

GI number: 116493078

Start: 1319387

End: 1320466

Strand: Reverse

Name: prfA [H]

Synonym: PEPE_1330

Alternate gene names: 116493078

Gene position: 1320466-1319387 (Counterclockwise)

Preceding gene: 116493079

Following gene: 116493077

Centisome position: 72.06

GC content: 38.8

Gene sequence:

>1080_bases
ATGGATATTATTGAAAAAGTTCAGGCTGTTGCCGACCGCTATGAAGAATTAAGCGAACTATTAGCTGATCCTGAAGTTCT
AGGAGATTCACAACGTTATCAAGAGCTTTCTAAAGAAGAAGGCGAAATCCGTGAAACTGTGGAAAAGTATCGTGAATACC
GAAAAACAGTTGATGCTATTGCTGAAAACGAAGAATTACAACGTGAAAAATTAGATCCAGAAATGGATAGTTTAGTTAAA
GCTGAATTAGCTGAACTCCGTGAAACTAAAGCTACTTTAGAAGAAGAAATTAAAATTTTAATGTTACCAACTGACCCCAA
TGATGATAAAAATATCATCATGGAAATTCATGGAGCAGCTGGTGGGGATGAAGCTAGTTTATTTGCTGCTGACCTATACA
ACATGTATACGCGTTATGCTGAAACCCAAGGATGGAAAGTAGAAGTTGTGGATGAAAATGCTACTGACGTTGGTGGTTTC
AAGGAAATCGTATTAATGATTTCTGGTAACAAAGTTTATTCTAAATTGAAGTATGAAAATGGTGCACATCGAGTTCAACG
GGTTCCAGTTACTGAATCGGCAGGACGAGTACATACATCTACATCAACCGTAGGAGTAATGCCTGAAGCGGAAGATGTTG
ATATTGAAATCGATCAAAAAGATATTCGTACGGATGTTTATCGCTCATCTGGTGCTGGTGGACAGCATATTAATAAGACT
TCTTCAGCGGTTCGAATGACCCACTTACCAACTGGTATCGTAGTTGCGATGCAAGATGAACGTTCGCAACAACAAAATCG
TGAAAAGGCAATGCGCATTTTAAAGGCTCGGGTCTATGACTATTACCAAACGCAAGAAGAAGATGCATATAATGCAGAAC
GTAAAAGCGCTGTTGGTACCGGAGATCGTTCCGAACGTATAAGAACTTACAACTTCCCTCAAAATCGGGTTACCGATCAT
CGGATTGGTCTTACTTTGAACAAGTTAGATCGAGTTATGAATGGTGAATTAGATGATATCATCGATGCTCTTATTCTCGA
TGATCAAGCCAAGAAAGTGGAACAACTTCGTAATGAATAA

Upstream 100 bases:

>100_bases
GGTGAGCAAGTTCAAATTGGTGGTAATGAGTCTTATTACCCGGTTTGTCGTCGCCATTATTTTCACCCACTGACTGAAGA
AGAAATGAGGAATTAATTAA

Downstream 100 bases:

>100_bases
TATTCCAACTTATTTTGAAGCCCTGAGATGGGCTTCTTTGTTTATCCGAAAAAACCAAGGAGACGAGAATGCTCCAGAAT
TGATCTTGATGGATACAATG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MDIIEKVQAVADRYEELSELLADPEVLGDSQRYQELSKEEGEIRETVEKYREYRKTVDAIAENEELQREKLDPEMDSLVK
AELAELRETKATLEEEIKILMLPTDPNDDKNIIMEIHGAAGGDEASLFAADLYNMYTRYAETQGWKVEVVDENATDVGGF
KEIVLMISGNKVYSKLKYENGAHRVQRVPVTESAGRVHTSTSTVGVMPEAEDVDIEIDQKDIRTDVYRSSGAGGQHINKT
SSAVRMTHLPTGIVVAMQDERSQQQNREKAMRILKARVYDYYQTQEEDAYNAERKSAVGTGDRSERIRTYNFPQNRVTDH
RIGLTLNKLDRVMNGELDDIIDALILDDQAKKVEQLRNE

Sequences:

>Translated_359_residues
MDIIEKVQAVADRYEELSELLADPEVLGDSQRYQELSKEEGEIRETVEKYREYRKTVDAIAENEELQREKLDPEMDSLVK
AELAELRETKATLEEEIKILMLPTDPNDDKNIIMEIHGAAGGDEASLFAADLYNMYTRYAETQGWKVEVVDENATDVGGF
KEIVLMISGNKVYSKLKYENGAHRVQRVPVTESAGRVHTSTSTVGVMPEAEDVDIEIDQKDIRTDVYRSSGAGGQHINKT
SSAVRMTHLPTGIVVAMQDERSQQQNREKAMRILKARVYDYYQTQEEDAYNAERKSAVGTGDRSERIRTYNFPQNRVTDH
RIGLTLNKLDRVMNGELDDIIDALILDDQAKKVEQLRNE
>Mature_359_residues
MDIIEKVQAVADRYEELSELLADPEVLGDSQRYQELSKEEGEIRETVEKYREYRKTVDAIAENEELQREKLDPEMDSLVK
AELAELRETKATLEEEIKILMLPTDPNDDKNIIMEIHGAAGGDEASLFAADLYNMYTRYAETQGWKVEVVDENATDVGGF
KEIVLMISGNKVYSKLKYENGAHRVQRVPVTESAGRVHTSTSTVGVMPEAEDVDIEIDQKDIRTDVYRSSGAGGQHINKT
SSAVRMTHLPTGIVVAMQDERSQQQNREKAMRILKARVYDYYQTQEEDAYNAERKSAVGTGDRSERIRTYNFPQNRVTDH
RIGLTLNKLDRVMNGELDDIIDALILDDQAKKVEQLRNE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=300, Percent_Identity=42.6666666666667, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI34577120, Length=354, Percent_Identity=35.8757062146893, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI166795305, Length=205, Percent_Identity=40.4878048780488, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=46.7605633802817, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI2367172, Length=342, Percent_Identity=37.4269005847953, Blast_Score=214, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=323, Percent_Identity=33.4365325077399, Blast_Score=181, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=329, Percent_Identity=38.9057750759878, Blast_Score=232, Evalue=6e-62,
Organism=Drosophila melanogaster, GI19921226, Length=339, Percent_Identity=40.117994100295, Blast_Score=240, Evalue=8e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40854; Mature: 40854

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIIEKVQAVADRYEELSELLADPEVLGDSQRYQELSKEEGEIRETVEKYREYRKTVDAI
CCHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AENEELQREKLDPEMDSLVKAELAELRETKATLEEEIKILMLPTDPNDDKNIIMEIHGAA
HCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECCC
GGDEASLFAADLYNMYTRYAETQGWKVEVVDENATDVGGFKEIVLMISGNKVYSKLKYEN
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEEEECCHHHHHHHCCC
GAHRVQRVPVTESAGRVHTSTSTVGVMPEAEDVDIEIDQKDIRTDVYRSSGAGGQHINKT
CHHHEEECCCCCCCCCEEECCCCEECCCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCCH
SSAVRMTHLPTGIVVAMQDERSQQQNREKAMRILKARVYDYYQTQEEDAYNAERKSAVGT
HHHEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHCCCC
GDRSERIRTYNFPQNRVTDHRIGLTLNKLDRVMNGELDDIIDALILDDQAKKVEQLRNE
CCCCCCCEECCCCCCCCCCHHHCCCHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCC
>Mature Secondary Structure
MDIIEKVQAVADRYEELSELLADPEVLGDSQRYQELSKEEGEIRETVEKYREYRKTVDAI
CCHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AENEELQREKLDPEMDSLVKAELAELRETKATLEEEIKILMLPTDPNDDKNIIMEIHGAA
HCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECCC
GGDEASLFAADLYNMYTRYAETQGWKVEVVDENATDVGGFKEIVLMISGNKVYSKLKYEN
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEEEECCHHHHHHHCCC
GAHRVQRVPVTESAGRVHTSTSTVGVMPEAEDVDIEIDQKDIRTDVYRSSGAGGQHINKT
CHHHEEECCCCCCCCCEEECCCCEECCCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCCH
SSAVRMTHLPTGIVVAMQDERSQQQNREKAMRILKARVYDYYQTQEEDAYNAERKSAVGT
HHHEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHCCCC
GDRSERIRTYNFPQNRVTDHRIGLTLNKLDRVMNGELDDIIDALILDDQAKKVEQLRNE
CCCCCCCEECCCCCCCCCCHHHCCCHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA