Click here to switch to the map view.

The map label for this gene is folC [H]

Identifier: 116493038

GI number: 116493038

Start: 1280055

End: 1281329

Strand: Reverse

Name: folC [H]

Synonym: PEPE_1290

Alternate gene names: 116493038

Gene position: 1281329-1280055 (Counterclockwise)

Preceding gene: 116493039

Following gene: 116493037

Centisome position: 69.93

GC content: 37.65

Gene sequence:

>1275_bases
ATGGATTATAAAACGGCTTTGAAATTTATACACGGGCGGCCGCGGTTACGAAAAGAGCCGACCGTAAAAAGAATGACTGC
CTTTCTAGATCAGTTAGGGAATCCACAAGATGCAATTCAAGGAATTCATGTTGCAGGGACCAATGGTAAAGGTTCGACCA
TTGCTTTTTTGGCCAAAATGATGGAGACTACTGGACAACGAGTAGGGACATTTACTTCGCCTTATTTAGAACGCTTCAAT
GAGCGGATTGCAGTAAATGGAATCCCTATCCGTGACGATGAGATTCTAACGTTAGTGGAAGAAATCAAACCAATCGTAGA
CGAGTTAGATCAAACTCCCTTAGGTGGACCATTAGAATTCGAAATTGTCACAGCCATGATGTTTTTGTATTTTAAAAAAC
ACCCCGTGGATATAGTTTTGATTGAAGTAGGTATTGGAGGAAGGTATGATTCTACAAATGTCTTCACCCCGATTGCTAGT
GTAATAACGAATATTGGTTGGGATCATATGCAAATTCTGGGTGATACATTACCTAAAATTGCTTTTCAAAAAGCAGGCAT
TATTAAAACTAATGTTCCAGTAATCACTGGAACTGAAGATCAGGCTGCGCTACGAGTGATTGAAGAGGAAGCTAAAAAGT
TACATGCACCGCTAAAAATTTTGAAACGTGATTTTAAAATTGATTCCAAGGAAAATTTTTTCAGCAATACGTTCGCTCAA
ATCGAACATATCCACAGTGGCTTACTGGGTTTATATCAAGTAAATAATTTAGCAGTTGCTCTAGAAACTATGTTAGTAGT
TAGTCGTCAATTAAATTGGAAGATTAACTATGATCAATTACGACAAGCAGTCCAACATACTCAATGGGCTGGCCGAATGG
AAATTATTCAAAAACGGCCTAAAATTATTTTAGATGGGGCCCATAATTTACCAGGAATCCAAGCATTAAAAAAATCGATT
CAAGCGTATTGGCCAGAACAAAAAGTTTTTATTTTAGCAGCTATTTTAGATGACAAAAGATTTGAGGCAATGTTGACCGA
GTTAGTTGATTTAAAACAAGTAGAGTTAACGTTAACTAGATTTAATGGGCCAATCAAGCGTGCTGCTGTAGACTGGTCAG
AACTGCACGATCCGATTCGAAAAGATATTAATTATCAAGCGAATTGGCAGTTGGCTTTAAAAGAAATTCTGCGTCAGGCT
AAACCTAGCGACGTAATTATTATTACCGGATCCTTGTATTTTATTTCTGAGGTACGCCCGCAGCTAATTAAATAA

Upstream 100 bases:

>100_bases
AATCTGAAAGCAAGTAGCTAACGTTTAAATTAACATTAAAGAACTGCAAACAAAAAGTGATTTTTGATGCGGTTCTTTTT
TGCTAGAAGGGGGCTCAGTG

Downstream 100 bases:

>100_bases
AGCCGGCTGAATTTCCGTATGTATTTATTAGAGGTGATGTATATGGAAATTCAAGATGAAGCGAAAGCAAAATTAAAAAA
AGTCGGTGCTGAAAATTTAA

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MDYKTALKFIHGRPRLRKEPTVKRMTAFLDQLGNPQDAIQGIHVAGTNGKGSTIAFLAKMMETTGQRVGTFTSPYLERFN
ERIAVNGIPIRDDEILTLVEEIKPIVDELDQTPLGGPLEFEIVTAMMFLYFKKHPVDIVLIEVGIGGRYDSTNVFTPIAS
VITNIGWDHMQILGDTLPKIAFQKAGIIKTNVPVITGTEDQAALRVIEEEAKKLHAPLKILKRDFKIDSKENFFSNTFAQ
IEHIHSGLLGLYQVNNLAVALETMLVVSRQLNWKINYDQLRQAVQHTQWAGRMEIIQKRPKIILDGAHNLPGIQALKKSI
QAYWPEQKVFILAAILDDKRFEAMLTELVDLKQVELTLTRFNGPIKRAAVDWSELHDPIRKDINYQANWQLALKEILRQA
KPSDVIIITGSLYFISEVRPQLIK

Sequences:

>Translated_424_residues
MDYKTALKFIHGRPRLRKEPTVKRMTAFLDQLGNPQDAIQGIHVAGTNGKGSTIAFLAKMMETTGQRVGTFTSPYLERFN
ERIAVNGIPIRDDEILTLVEEIKPIVDELDQTPLGGPLEFEIVTAMMFLYFKKHPVDIVLIEVGIGGRYDSTNVFTPIAS
VITNIGWDHMQILGDTLPKIAFQKAGIIKTNVPVITGTEDQAALRVIEEEAKKLHAPLKILKRDFKIDSKENFFSNTFAQ
IEHIHSGLLGLYQVNNLAVALETMLVVSRQLNWKINYDQLRQAVQHTQWAGRMEIIQKRPKIILDGAHNLPGIQALKKSI
QAYWPEQKVFILAAILDDKRFEAMLTELVDLKQVELTLTRFNGPIKRAAVDWSELHDPIRKDINYQANWQLALKEILRQA
KPSDVIIITGSLYFISEVRPQLIK
>Mature_424_residues
MDYKTALKFIHGRPRLRKEPTVKRMTAFLDQLGNPQDAIQGIHVAGTNGKGSTIAFLAKMMETTGQRVGTFTSPYLERFN
ERIAVNGIPIRDDEILTLVEEIKPIVDELDQTPLGGPLEFEIVTAMMFLYFKKHPVDIVLIEVGIGGRYDSTNVFTPIAS
VITNIGWDHMQILGDTLPKIAFQKAGIIKTNVPVITGTEDQAALRVIEEEAKKLHAPLKILKRDFKIDSKENFFSNTFAQ
IEHIHSGLLGLYQVNNLAVALETMLVVSRQLNWKINYDQLRQAVQHTQWAGRMEIIQKRPKIILDGAHNLPGIQALKKSI
QAYWPEQKVFILAAILDDKRFEAMLTELVDLKQVELTLTRFNGPIKRAAVDWSELHDPIRKDINYQANWQLALKEILRQA
KPSDVIIITGSLYFISEVRPQLIK

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=353, Percent_Identity=30.028328611898, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI66932990, Length=353, Percent_Identity=30.028328611898, Blast_Score=147, Evalue=1e-35,
Organism=Escherichia coli, GI1788654, Length=378, Percent_Identity=26.984126984127, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71984923, Length=316, Percent_Identity=29.4303797468354, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17553150, Length=316, Percent_Identity=29.4303797468354, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17553148, Length=316, Percent_Identity=29.4303797468354, Blast_Score=134, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6323760, Length=429, Percent_Identity=29.6037296037296, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6324815, Length=295, Percent_Identity=29.8305084745763, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322718, Length=434, Percent_Identity=23.2718894009217, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24641571, Length=311, Percent_Identity=32.1543408360129, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24581568, Length=209, Percent_Identity=30.1435406698565, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48078; Mature: 48078

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYKTALKFIHGRPRLRKEPTVKRMTAFLDQLGNPQDAIQGIHVAGTNGKGSTIAFLAKM
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCEEEEECCCCCCHHHHHHHHH
METTGQRVGTFTSPYLERFNERIAVNGIPIRDDEILTLVEEIKPIVDELDQTPLGGPLEF
HHHHCHHCCCCCHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EIVTAMMFLYFKKHPVDIVLIEVGIGGRYDSTNVFTPIASVITNIGWDHMQILGDTLPKI
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
AFQKAGIIKTNVPVITGTEDQAALRVIEEEAKKLHAPLKILKRDFKIDSKENFFSNTFAQ
HHHHCCCEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IEHIHSGLLGLYQVNNLAVALETMLVVSRQLNWKINYDQLRQAVQHTQWAGRMEIIQKRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHCCC
KIILDGAHNLPGIQALKKSIQAYWPEQKVFILAAILDDKRFEAMLTELVDLKQVELTLTR
CEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHEEEEEC
FNGPIKRAAVDWSELHDPIRKDINYQANWQLALKEILRQAKPSDVIIITGSLYFISEVRP
CCCCHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHH
QLIK
HHCC
>Mature Secondary Structure
MDYKTALKFIHGRPRLRKEPTVKRMTAFLDQLGNPQDAIQGIHVAGTNGKGSTIAFLAKM
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCEEEEECCCCCCHHHHHHHHH
METTGQRVGTFTSPYLERFNERIAVNGIPIRDDEILTLVEEIKPIVDELDQTPLGGPLEF
HHHHCHHCCCCCHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHH
EIVTAMMFLYFKKHPVDIVLIEVGIGGRYDSTNVFTPIASVITNIGWDHMQILGDTLPKI
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
AFQKAGIIKTNVPVITGTEDQAALRVIEEEAKKLHAPLKILKRDFKIDSKENFFSNTFAQ
HHHHCCCEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IEHIHSGLLGLYQVNNLAVALETMLVVSRQLNWKINYDQLRQAVQHTQWAGRMEIIQKRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHCCC
KIILDGAHNLPGIQALKKSIQAYWPEQKVFILAAILDDKRFEAMLTELVDLKQVELTLTR
CEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHEEEEEC
FNGPIKRAAVDWSELHDPIRKDINYQANWQLALKEILRQAKPSDVIIITGSLYFISEVRP
CCCCHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHH
QLIK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]