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The map label for this gene is glnH [H]

Identifier: 116493032

GI number: 116493032

Start: 1275275

End: 1276096

Strand: Reverse

Name: glnH [H]

Synonym: PEPE_1284

Alternate gene names: 116493032

Gene position: 1276096-1275275 (Counterclockwise)

Preceding gene: 116493033

Following gene: 116493031

Centisome position: 69.64

GC content: 33.94

Gene sequence:

>822_bases
ATGAAAAAAGAATTGAAATTATTTCTTGTGATGGCTTTGGGACTATTAATTATTCCAGTTCTAGGAGGTTGTGGCAATTC
AAAGAAAACCACGAGGGCCGAACCTAGTAGTTGGGAAACTATTCAAAAAAATAAAAAAGTCGTAATTGGCTTAGATGATA
GTTTTGTACCAATGGGATTTCGAGAAAAAAATGGGCAGCTTGCTGGATACGATATTGATTTAGCTAGAGCAGTTTTTAGA
CAATATGGAATTAAAGTTGATTTTCAAACAATTGATTGGTCAATGAACGTAACTGAATTGCGCAATCAAACGATTGATTT
GATTTGGAATGGATTAACAATCACTGATGAACGTCAAAAAGCGTTGACGCTAAGTGAGCCTTATCTAACTAATCAACAAA
TTATAGTAGTAAAGAAAAGTAGCGGGATTACCAAGTTAGCAGATCTGAAAAATAAAAGTGTTGGTGCACAAAGTGGGTCA
TCGGGGGCACAAGATATTGATGACCATCCCCAAATTTTAAAGAATCGGATTAAAAACCACGACCCTATTTTGTATGATAG
TTTCAATAACGCGTTTATTGATTTGAATGCTAACCGTATCCAAGGGTTACTAATTGATAGTGTGTATGCTAATTACTATA
TTAAGCATATGTCGAATTCAAGTGATTATCGGATTATAAAGAGTAATATGCCCAGTGAACAATTTGCAGTTGGAATTAAA
AAAGGCAACACTGAAGTTAAACAAAAAATTGATCAGGGAATTAATCAATTAAGGGCCGATGGAGAATTAAAACGTATCAA
TCAAAAGTGGTTTGGAAAATAA

Upstream 100 bases:

>100_bases
CTTGAAGGAAAATGGTATTACACAAGTAATTATTACGCATGATATTGAATTTGCAGATAAAGTTGCTGATGATACTTTAA
CAGTGAAGCCGGTGGATTAA

Downstream 100 bases:

>100_bases
TCTGAACCTTAAGACCGAAAATTTAGTTATTTTCGGTCTTTTTGATACACTGGAGAAAAGAATGGCGGAGGAAGATCATT
TTGGTTCTTATTGAGATTGT

Product: amino acid ABC transporter periplasmic protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKKELKLFLVMALGLLIIPVLGGCGNSKKTTRAEPSSWETIQKNKKVVIGLDDSFVPMGFREKNGQLAGYDIDLARAVFR
QYGIKVDFQTIDWSMNVTELRNQTIDLIWNGLTITDERQKALTLSEPYLTNQQIIVVKKSSGITKLADLKNKSVGAQSGS
SGAQDIDDHPQILKNRIKNHDPILYDSFNNAFIDLNANRIQGLLIDSVYANYYIKHMSNSSDYRIIKSNMPSEQFAVGIK
KGNTEVKQKIDQGINQLRADGELKRINQKWFGK

Sequences:

>Translated_273_residues
MKKELKLFLVMALGLLIIPVLGGCGNSKKTTRAEPSSWETIQKNKKVVIGLDDSFVPMGFREKNGQLAGYDIDLARAVFR
QYGIKVDFQTIDWSMNVTELRNQTIDLIWNGLTITDERQKALTLSEPYLTNQQIIVVKKSSGITKLADLKNKSVGAQSGS
SGAQDIDDHPQILKNRIKNHDPILYDSFNNAFIDLNANRIQGLLIDSVYANYYIKHMSNSSDYRIIKSNMPSEQFAVGIK
KGNTEVKQKIDQGINQLRADGELKRINQKWFGK
>Mature_273_residues
MKKELKLFLVMALGLLIIPVLGGCGNSKKTTRAEPSSWETIQKNKKVVIGLDDSFVPMGFREKNGQLAGYDIDLARAVFR
QYGIKVDFQTIDWSMNVTELRNQTIDLIWNGLTITDERQKALTLSEPYLTNQQIIVVKKSSGITKLADLKNKSVGAQSGS
SGAQDIDDHPQILKNRIKNHDPILYDSFNNAFIDLNANRIQGLLIDSVYANYYIKHMSNSSDYRIIKSNMPSEQFAVGIK
KGNTEVKQKIDQGINQLRADGELKRINQKWFGK

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=230, Percent_Identity=29.1304347826087, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1788228, Length=268, Percent_Identity=25.7462686567164, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787085, Length=230, Percent_Identity=26.5217391304348, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787088, Length=224, Percent_Identity=27.2321428571429, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788649, Length=271, Percent_Identity=25.4612546125461, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=24.4635193133047, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1786876, Length=248, Percent_Identity=22.1774193548387, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30768; Mature: 30768

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKELKLFLVMALGLLIIPVLGGCGNSKKTTRAEPSSWETIQKNKKVVIGLDDSFVPMGF
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCE
REKNGQLAGYDIDLARAVFRQYGIKVDFQTIDWSMNVTELRNQTIDLIWNGLTITDERQK
ECCCCCEECCCHHHHHHHHHHHCCEEEEEEEECCCCHHHHHCCEEEEEECCEEEECCCHH
ALTLSEPYLTNQQIIVVKKSSGITKLADLKNKSVGAQSGSSGAQDIDDHPQILKNRIKNH
EEEECCCCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
DPILYDSFNNAFIDLNANRIQGLLIDSVYANYYIKHMSNSSDYRIIKSNMPSEQFAVGIK
CCEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCHHHEEEEEE
KGNTEVKQKIDQGINQLRADGELKRINQKWFGK
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKKELKLFLVMALGLLIIPVLGGCGNSKKTTRAEPSSWETIQKNKKVVIGLDDSFVPMGF
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCE
REKNGQLAGYDIDLARAVFRQYGIKVDFQTIDWSMNVTELRNQTIDLIWNGLTITDERQK
ECCCCCEECCCHHHHHHHHHHHCCEEEEEEEECCCCHHHHHCCEEEEEECCEEEECCCHH
ALTLSEPYLTNQQIIVVKKSSGITKLADLKNKSVGAQSGSSGAQDIDDHPQILKNRIKNH
EEEECCCCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
DPILYDSFNNAFIDLNANRIQGLLIDSVYANYYIKHMSNSSDYRIIKSNMPSEQFAVGIK
CCEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCHHHEEEEEE
KGNTEVKQKIDQGINQLRADGELKRINQKWFGK
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]