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The map label for this gene is ywnE [H]

Identifier: 116493031

GI number: 116493031

Start: 1273767

End: 1275194

Strand: Reverse

Name: ywnE [H]

Synonym: PEPE_1283

Alternate gene names: 116493031

Gene position: 1275194-1273767 (Counterclockwise)

Preceding gene: 116493032

Following gene: 116493030

Centisome position: 69.59

GC content: 34.17

Gene sequence:

>1428_bases
TTGGTTCTTATTGAGATTGTTTTTTATCTAATCAATATTATCATCGCGTTAACGATCGCATTAATGGAAAAGCGAGATAT
TTCTGCAGCATGGGCTTGGTTATTTGTAATGCTATTGCTTCCTGGAATTGGGTTTATAATTTATCTATTTTTTGGCTGGA
AGTTAAATCAAAGGCAAATATTTGATTTGAAAGCTCAAAAAAGACTCGGAATTAGCGATATGGCTGAATATCAAAAGCGT
AATCCTAAAAACAAACCGACCCTGGATACCAATCTTGAAAATGACTTGGTTCAGATGTTTTTAAACACGGATAATGCAAT
TTTAACGACTAAAAATGATTTAAAGATTTTTACAGATGGTCATGAAAAATTTAACAGCTTGTTTGAAGATTTAAAAAAGG
CTAAACATCATATCAATATCGAATATTTTACAATCTATGATGATCAGTTAGGCAAAAAATTACGTAAAATTTTGGTTCAA
AAAGCGCGTGAAGGTGTCCAAGTAAGGGTGTTGTACGATTTATTTGGATCAAAAGGATCCAAGCAAAAATTTTTTAAAGA
ATTAATTCAGGCTGGTGGCGAAGTCACGCCATTTATGAAATCTAAACTAGGCTATTATAGTTTTAGAATTAATTTTCGAA
ATCATCGAAAAATTGTGGTGATTGATGGATCGGTTGGATACATTGGTGGTTTTAACATTGGTGACCAATATTTAGGACGT
AATAAGCGCTTTGGAAACTGGCGTGATACGCACTTAAGGTTAGAGGGTTCCGTGGTATTGCAATTGCAAAGTCGTTTTTT
CATGGATTGGAATGCATCTGCTAAGCGTCAAAAAGTTCAATTTAGCTTAGATTATTTTCCACAAAGTAACGTTCAAAATA
ATATTCCAATGCAGATTGTTAGTAGTGGACCTGAGAATGATGTTCAAAAAATCAAACAGGGTTATATTAAAATGATTATG
GGGGCTAAACATTCCATTTGGATTGAAACGCCATACTTTATCCCTGATGATGCCTTAATGGAAGCTTTGTTAATCGCTAT
TCGTTCAGGCATCGAAGTGCGGATTGTAATTCCACAAATGCCTGATCATCCCTTCGTCTACCGGGCTACAGAGTATTATG
TACAACAATTGTTAAAAGCTGGCGCGCGTATCTATTCATATCAAAATGGGTTCATGCATGCTAAGACGATTGTGGTCGAT
AATATGATTACAAGTGTAGGTTCAGCAAATTGGGATATTCGTTCTTTTAAATTAAATTTTGAAGCAAATGCATTTATGTA
TGATCCTAAAGTGGCTGAACAAATAATCGCTACAATTAAAAATGATTTAAAGGATGCGCGTGAATTGGATGAAGAATATT
TCAAGCAACAATCTTCGTGGAAAAAATTCCGTCAATTGGCTAGTCGTTTGATTTCACCAATTCTTTAA

Upstream 100 bases:

>100_bases
AAAAGTGGTTTGGAAAATAATCTGAACCTTAAGACCGAAAATTTAGTTATTTTCGGTCTTTTTGATACACTGGAGAAAAG
AATGGCGGAGGAAGATCATT

Downstream 100 bases:

>100_bases
TTAACATTGAAAAGTGTGCGAAATTTCGCTAACATTAATCGCATGTGAAAAAGTAGGAGGCATCATATTGGATGTTCAAA
ATCAGTCTGGGATTCAGACT

Product: cardiolipin synthetase 2

Products: NA

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MVLIEIVFYLINIIIALTIALMEKRDISAAWAWLFVMLLLPGIGFIIYLFFGWKLNQRQIFDLKAQKRLGISDMAEYQKR
NPKNKPTLDTNLENDLVQMFLNTDNAILTTKNDLKIFTDGHEKFNSLFEDLKKAKHHINIEYFTIYDDQLGKKLRKILVQ
KAREGVQVRVLYDLFGSKGSKQKFFKELIQAGGEVTPFMKSKLGYYSFRINFRNHRKIVVIDGSVGYIGGFNIGDQYLGR
NKRFGNWRDTHLRLEGSVVLQLQSRFFMDWNASAKRQKVQFSLDYFPQSNVQNNIPMQIVSSGPENDVQKIKQGYIKMIM
GAKHSIWIETPYFIPDDALMEALLIAIRSGIEVRIVIPQMPDHPFVYRATEYYVQQLLKAGARIYSYQNGFMHAKTIVVD
NMITSVGSANWDIRSFKLNFEANAFMYDPKVAEQIIATIKNDLKDARELDEEYFKQQSSWKKFRQLASRLISPIL

Sequences:

>Translated_475_residues
MVLIEIVFYLINIIIALTIALMEKRDISAAWAWLFVMLLLPGIGFIIYLFFGWKLNQRQIFDLKAQKRLGISDMAEYQKR
NPKNKPTLDTNLENDLVQMFLNTDNAILTTKNDLKIFTDGHEKFNSLFEDLKKAKHHINIEYFTIYDDQLGKKLRKILVQ
KAREGVQVRVLYDLFGSKGSKQKFFKELIQAGGEVTPFMKSKLGYYSFRINFRNHRKIVVIDGSVGYIGGFNIGDQYLGR
NKRFGNWRDTHLRLEGSVVLQLQSRFFMDWNASAKRQKVQFSLDYFPQSNVQNNIPMQIVSSGPENDVQKIKQGYIKMIM
GAKHSIWIETPYFIPDDALMEALLIAIRSGIEVRIVIPQMPDHPFVYRATEYYVQQLLKAGARIYSYQNGFMHAKTIVVD
NMITSVGSANWDIRSFKLNFEANAFMYDPKVAEQIIATIKNDLKDARELDEEYFKQQSSWKKFRQLASRLISPIL
>Mature_475_residues
MVLIEIVFYLINIIIALTIALMEKRDISAAWAWLFVMLLLPGIGFIIYLFFGWKLNQRQIFDLKAQKRLGISDMAEYQKR
NPKNKPTLDTNLENDLVQMFLNTDNAILTTKNDLKIFTDGHEKFNSLFEDLKKAKHHINIEYFTIYDDQLGKKLRKILVQ
KAREGVQVRVLYDLFGSKGSKQKFFKELIQAGGEVTPFMKSKLGYYSFRINFRNHRKIVVIDGSVGYIGGFNIGDQYLGR
NKRFGNWRDTHLRLEGSVVLQLQSRFFMDWNASAKRQKVQFSLDYFPQSNVQNNIPMQIVSSGPENDVQKIKQGYIKMIM
GAKHSIWIETPYFIPDDALMEALLIAIRSGIEVRIVIPQMPDHPFVYRATEYYVQQLLKAGARIYSYQNGFMHAKTIVVD
NMITSVGSANWDIRSFKLNFEANAFMYDPKVAEQIIATIKNDLKDARELDEEYFKQQSSWKKFRQLASRLISPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=490, Percent_Identity=25.3061224489796, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI1787007, Length=372, Percent_Identity=26.6129032258064, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 55221; Mature: 55221

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLIEIVFYLINIIIALTIALMEKRDISAAWAWLFVMLLLPGIGFIIYLFFGWKLNQRQI
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
FDLKAQKRLGISDMAEYQKRNPKNKPTLDTNLENDLVQMFLNTDNAILTTKNDLKIFTDG
EECHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEECC
HEKFNSLFEDLKKAKHHINIEYFTIYDDQLGKKLRKILVQKAREGVQVRVLYDLFGSKGS
HHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCC
KQKFFKELIQAGGEVTPFMKSKLGYYSFRINFRNHRKIVVIDGSVGYIGGFNIGDQYLGR
HHHHHHHHHHCCCCCCHHHHHCCCEEEEEEEECCCCEEEEEECCCCEECCCCCCHHHHCC
NKRFGNWRDTHLRLEGSVVLQLQSRFFMDWNASAKRQKVQFSLDYFPQSNVQNNIPMQIV
CCCCCCCCCCEEEECCCEEEEEEHHEEECCCCCCCCEEEEEEEECCCCCCCCCCCCEEEE
SSGPENDVQKIKQGYIKMIMGAKHSIWIETPYFIPDDALMEALLIAIRSGIEVRIVIPQM
CCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCC
PDHPFVYRATEYYVQQLLKAGARIYSYQNGFMHAKTIVVDNMITSVGSANWDIRSFKLNF
CCCCEEEHHHHHHHHHHHHCCHHEEEECCCCEEHHHHHHHHHHHHCCCCCCCEEEEEEEE
EANAFMYDPKVAEQIIATIKNDLKDARELDEEYFKQQSSWKKFRQLASRLISPIL
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVLIEIVFYLINIIIALTIALMEKRDISAAWAWLFVMLLLPGIGFIIYLFFGWKLNQRQI
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
FDLKAQKRLGISDMAEYQKRNPKNKPTLDTNLENDLVQMFLNTDNAILTTKNDLKIFTDG
EECHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEEECC
HEKFNSLFEDLKKAKHHINIEYFTIYDDQLGKKLRKILVQKAREGVQVRVLYDLFGSKGS
HHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCC
KQKFFKELIQAGGEVTPFMKSKLGYYSFRINFRNHRKIVVIDGSVGYIGGFNIGDQYLGR
HHHHHHHHHHCCCCCCHHHHHCCCEEEEEEEECCCCEEEEEECCCCEECCCCCCHHHHCC
NKRFGNWRDTHLRLEGSVVLQLQSRFFMDWNASAKRQKVQFSLDYFPQSNVQNNIPMQIV
CCCCCCCCCCEEEECCCEEEEEEHHEEECCCCCCCCEEEEEEEECCCCCCCCCCCCEEEE
SSGPENDVQKIKQGYIKMIMGAKHSIWIETPYFIPDDALMEALLIAIRSGIEVRIVIPQM
CCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCC
PDHPFVYRATEYYVQQLLKAGARIYSYQNGFMHAKTIVVDNMITSVGSANWDIRSFKLNF
CCCCEEEHHHHHHHHHHHHCCHHEEEECCCCEEHHHHHHHHHHHHCCCCCCCEEEEEEEE
EANAFMYDPKVAEQIIATIKNDLKDARELDEEYFKQQSSWKKFRQLASRLISPIL
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]