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The map label for this gene is dinB [H]

Identifier: 116493014

GI number: 116493014

Start: 1252971

End: 1254095

Strand: Reverse

Name: dinB [H]

Synonym: PEPE_1266

Alternate gene names: 116493014

Gene position: 1254095-1252971 (Counterclockwise)

Preceding gene: 116493015

Following gene: 116493013

Centisome position: 68.44

GC content: 39.2

Gene sequence:

>1125_bases
ATGGAAACAGAAGTTTTAACTCCGATCAACATTGATGATTCTCGTAAGATAATTCATGTTGATATGGACGCCTTTTATGC
TTCAATTGAGATGCGAGATAACCCCAAGTTCAAAACCCAGCCTTTGGTGGTAGCTCATGATCCGCGCCTTACTGGAGGAC
ATGGGGTTGTCACAACGGCAAACTATATTGCACGGCAATTTGGTGTCCATTCTGCGATGCCGGCTATGAAAGCTTTAGAA
CTTTGTCCAGAGGCGATTTTTAAAGATCCGGACTTCACTAAATATCGTGCAGTTTCGGAACAAATTCATCAAATTTTTCA
CGAGTATACCGATAAGATTGAACCCGTTGCCTTTGATGAGGCCTATTTAGATGTTTCAGAAGATAAAAAGGGGCTTGGTG
ACGCTGTGTTGATTGCTCATGAAATTCAGAAAGTTATCTTTAATGAAACCCAATTAACCTGCTCTACGGGAATTTCATAC
AATAAATTTCTGGCTAAAATGGCTTCCGAATATTCCAAGCCGGTAGGTGTGGCAATCATCGAAAAAGATGACGTTGCTGA
GTTTCTAAAGAATATTCCAATCGAACGGTTTAGAGGGGTTGGCAAAAAAACAGTGCCTAAAATGCACGAGCTAGGTGTTT
TTCAAGGTGCGGATTTAATCAAGATGTCTGAAAATGATTTAATTCATTACTTTGGAAAATTTGGGTACACCTTGTTTCGT
TGGGTTCGCGGACAAGATGATCGCCCAGTCGAATACTTGCGTGAAAGAAAATCAATTGGCAAAGAAGAAACGTATGGAAA
GTTGCTCACCACTGAGATGGAGGTTGAAGAGCGACTTCGGGTAATTGCTAAGAATCTATTTCGCGCAGTTTTGAAAAGAC
AGAAACACGGCAAAACCATTGTATTGAAACTAAGATATGGTGATTTTGAAACGTTAACTAAACGAATCACATTGGACGAA
TTTTTCCAAAATGATGTTGAAACCTATTATTTTTATGCTAAACAGATTTTGGATGAAATTGCTGATATTTCGCGGGGGAT
TCGCTTATTAGGGATTACACTAACCAACTTAACGGATCAAAATTTTCAAGAAGTTTCTTTACCACTGTTTAAGCACTCAG
AGTGA

Upstream 100 bases:

>100_bases
CATTTCCAAACTATGCAAGTGGTAGTATGGGACCTGAAGCAGCTATGCAACTTCTAGCTGATGATGGACGTAAATGGGTT
TTTAATCCACGCAGCTAATA

Downstream 100 bases:

>100_bases
TTTTGTGATATGCTTAAGAGCGCTAATAATAAAGTATTAAACTTATATTGATAAAAATTTAATGAGTAGGATAAATGAGG
CTGTAATGGACACACTTGAA

Product: nucleotidyltransferase/DNA polymerase for DNA repair

Products: NA

Alternate protein names: Pol IV [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
METEVLTPINIDDSRKIIHVDMDAFYASIEMRDNPKFKTQPLVVAHDPRLTGGHGVVTTANYIARQFGVHSAMPAMKALE
LCPEAIFKDPDFTKYRAVSEQIHQIFHEYTDKIEPVAFDEAYLDVSEDKKGLGDAVLIAHEIQKVIFNETQLTCSTGISY
NKFLAKMASEYSKPVGVAIIEKDDVAEFLKNIPIERFRGVGKKTVPKMHELGVFQGADLIKMSENDLIHYFGKFGYTLFR
WVRGQDDRPVEYLRERKSIGKEETYGKLLTTEMEVEERLRVIAKNLFRAVLKRQKHGKTIVLKLRYGDFETLTKRITLDE
FFQNDVETYYFYAKQILDEIADISRGIRLLGITLTNLTDQNFQEVSLPLFKHSE

Sequences:

>Translated_374_residues
METEVLTPINIDDSRKIIHVDMDAFYASIEMRDNPKFKTQPLVVAHDPRLTGGHGVVTTANYIARQFGVHSAMPAMKALE
LCPEAIFKDPDFTKYRAVSEQIHQIFHEYTDKIEPVAFDEAYLDVSEDKKGLGDAVLIAHEIQKVIFNETQLTCSTGISY
NKFLAKMASEYSKPVGVAIIEKDDVAEFLKNIPIERFRGVGKKTVPKMHELGVFQGADLIKMSENDLIHYFGKFGYTLFR
WVRGQDDRPVEYLRERKSIGKEETYGKLLTTEMEVEERLRVIAKNLFRAVLKRQKHGKTIVLKLRYGDFETLTKRITLDE
FFQNDVETYYFYAKQILDEIADISRGIRLLGITLTNLTDQNFQEVSLPLFKHSE
>Mature_374_residues
METEVLTPINIDDSRKIIHVDMDAFYASIEMRDNPKFKTQPLVVAHDPRLTGGHGVVTTANYIARQFGVHSAMPAMKALE
LCPEAIFKDPDFTKYRAVSEQIHQIFHEYTDKIEPVAFDEAYLDVSEDKKGLGDAVLIAHEIQKVIFNETQLTCSTGISY
NKFLAKMASEYSKPVGVAIIEKDDVAEFLKNIPIERFRGVGKKTVPKMHELGVFQGADLIKMSENDLIHYFGKFGYTLFR
WVRGQDDRPVEYLRERKSIGKEETYGKLLTTEMEVEERLRVIAKNLFRAVLKRQKHGKTIVLKLRYGDFETLTKRITLDE
FFQNDVETYYFYAKQILDEIADISRGIRLLGITLTNLTDQNFQEVSLPLFKHSE

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI84043967, Length=423, Percent_Identity=26.0047281323877, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI7706681, Length=424, Percent_Identity=25.9433962264151, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI7705344, Length=450, Percent_Identity=27.1111111111111, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI154350220, Length=335, Percent_Identity=26.2686567164179, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI5729982, Length=160, Percent_Identity=31.25, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1786425, Length=367, Percent_Identity=39.2370572207085, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1787432, Length=266, Percent_Identity=24.0601503759398, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI193205700, Length=411, Percent_Identity=27.4939172749392, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17537959, Length=300, Percent_Identity=28.6666666666667, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI193205702, Length=214, Percent_Identity=29.4392523364486, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115534089, Length=123, Percent_Identity=36.5853658536585, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19923006, Length=412, Percent_Identity=23.5436893203883, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI21355641, Length=317, Percent_Identity=26.4984227129338, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24644984, Length=317, Percent_Identity=26.4984227129338, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24668444, Length=121, Percent_Identity=33.0578512396694, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 43034; Mature: 43034

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METEVLTPINIDDSRKIIHVDMDAFYASIEMRDNPKFKTQPLVVAHDPRLTGGHGVVTTA
CCCCEECCCCCCCCCEEEEEEHHHHEEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHH
NYIARQFGVHSAMPAMKALELCPEAIFKDPDFTKYRAVSEQIHQIFHEYTDKIEPVAFDE
HHHHHHHCHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECH
AYLDVSEDKKGLGDAVLIAHEIQKVIFNETQLTCSTGISYNKFLAKMASEYSKPVGVAII
HHEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCEEEEE
EKDDVAEFLKNIPIERFRGVGKKTVPKMHELGVFQGADLIKMSENDLIHYFGKFGYTLFR
CHHHHHHHHHCCCHHHHHCCCCHHCCHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
WVRGQDDRPVEYLRERKSIGKEETYGKLLTTEMEVEERLRVIAKNLFRAVLKRQKHGKTI
HHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VLKLRYGDFETLTKRITLDEFFQNDVETYYFYAKQILDEIADISRGIRLLGITLTNLTDQ
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCC
NFQEVSLPLFKHSE
CHHHHCCCHHCCCC
>Mature Secondary Structure
METEVLTPINIDDSRKIIHVDMDAFYASIEMRDNPKFKTQPLVVAHDPRLTGGHGVVTTA
CCCCEECCCCCCCCCEEEEEEHHHHEEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHH
NYIARQFGVHSAMPAMKALELCPEAIFKDPDFTKYRAVSEQIHQIFHEYTDKIEPVAFDE
HHHHHHHCHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECH
AYLDVSEDKKGLGDAVLIAHEIQKVIFNETQLTCSTGISYNKFLAKMASEYSKPVGVAII
HHEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCEEEEE
EKDDVAEFLKNIPIERFRGVGKKTVPKMHELGVFQGADLIKMSENDLIHYFGKFGYTLFR
CHHHHHHHHHCCCHHHHHCCCCHHCCHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
WVRGQDDRPVEYLRERKSIGKEETYGKLLTTEMEVEERLRVIAKNLFRAVLKRQKHGKTI
HHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VLKLRYGDFETLTKRITLDEFFQNDVETYYFYAKQILDEIADISRGIRLLGITLTNLTDQ
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCC
NFQEVSLPLFKHSE
CHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]