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The map label for this gene is pbpB [H]

Identifier: 116492950

GI number: 116492950

Start: 1189834

End: 1191999

Strand: Reverse

Name: pbpB [H]

Synonym: PEPE_1189

Alternate gene names: 116492950

Gene position: 1191999-1189834 (Counterclockwise)

Preceding gene: 116492951

Following gene: 116492949

Centisome position: 65.05

GC content: 38.37

Gene sequence:

>2166_bases
ATGATGAACCGAAAAAAGCGAACAACTGGTACTACGGAAACTCAACACAATCGGAAAATGTTTGGTAAATGGCTATTATT
GATTGTGATTGGAGTATTTATAGTTATTGTTGGGCGCTTTTCTTATATTGCCGTAAGTGGCACTGTCGAAAGTGTTAACT
TAAGTAGTCAAGCAAAGCGTTTGTATACCGATAATAAAACAATTAAAGCCAAAAGAGGATCTATTTTAGATACGAATGGC
AATCCGATTGCCGAAGACACAAGTACATATTCAGTTTACGCAGTTGTAAGTAAGAAGCAGGTAGGAGCAAATAACAAGCC
GCTTTATGTCACCAATAAGAAAAAGACGGCTAAAGTACTTAGCAAATATCTACCAATTAGCTATCACAAGGCTTTAAAAA
CTCTTAGTCCCAGCAATAGTAAAACGTTCCAAGTTGAATTTGGAAATGCGGGAAGCAATATTTCTGTGGCGACTAAAGAA
AAAATAGAAAAAGAAAAATTAAACGGAATTAACTTTGTTTCATCTGAAGCTCGGTTATATCCCAATGGAACTTTTGCTTC
AAATTTGATTGGATTTGCTCAACAATCTCAACAAAAGGAAAATGATGCGACTCAACTTTCTGGCGTTATGGGAATTGAAA
AAATGAGTAACAAAACTTTAACGGGGACTGATGGTGAACAATCGTTGCAAAAAGATAAGTTTGGCTACCAGCTCCCAGGA
TCCCAGACTGAGCGTCCAGCACGCGACGGAAGCACAATCTATACTACTATTGACCAGCGTTTACAAAACATCATGGAAAA
TGTGGTAAGTAAGGTTCAATCAGAATCGGAATCACAGGCAATTAGCGCAACAATTATGAATGCCAAAACGGGAGCAATTT
TAGCCACAACACAACGACCAACCTTTAATGCTTCAACTAAAAAAGGACTTTCTAAAGCATGGAATAATGCGCTAGTTCAG
GATACTTTTGAACCAGGTTCAACCATGAAAATCTTTACTACTTCTGCGGCCATTGATAGTGGTAATTTTGACTCGGATGC
CACTTATCGTTCTGGAACCTATACCATCGATGGTAAAGTTGTTCCAGATTGGAAGACAGAAGGGTGGGGCGTAATTAGTT
ACGCAAAAGGATTTGCCTTATCAAGTAACGTCGCTATGGCTCACTTAGAACAAAATATGGGAGCTAAGACATGGAAGAAA
TACATTAATCGTTTTAAACTGTTGCAATCCACTAATTCTGGTTTGGAAAGCGAATCTAAAGGTAGTATTCAATTCGACTA
TCCGATTGAACAAGCTAATACAGCTTTCGGTCAAGCAATCAATGTGACCGCAATCCAGATGTTACAAGGTTTTACAGCAG
TTGCTAATAATGGAAAAATGGTTAAGCCTCAATTTATCAAAAAGGTAGTTGATCCGAATACTGGTAAAACAACTTATAAA
ATGAAGACAAAAGATGTAGGACGGCCAATTAAGGCTAGTACTGCTAAAAAGGTTCGAAAACTAATGGAGGATGTAGTATA
CAAACCATATGGTATCGGCTCAAACTTCAAAATTAAAGGTTATAAAATTGCAGCTAAAACAGGTACTGCCCAAGTTGCGA
ATGCAAGTGGAGGATATTCAATGGGCGATGACAGTTATCTGTATTCAGTCGCTGGGATGGCCCCGGCCAAAAATCCTAAG
TACATAATGTACATTACGATGAAAAAGCCAGTGCTCAAAGATAAAACTGCCACACAATTACTTGATGAAATATTTGGTCC
AGTGATGAAACAAGCCCTTGAACAGGATAAGGAAACTGATAAGTCAAAGCTCGACAGCTCAATTGAAGTACCTAATTTAG
TTGGCAAGGATGCTTCTGAAGCCAAAAAAGAATTGGGTAAGAAGAATGTGGCAGTTGTTACATTAGGAAATGGGCAAACT
GTTAAAGAACAATCCGAGAGTGCAGGAAGTAGTATTCTAGTGGGGCAACGAATTATCTTAGACACTGGTGGAACCATAAC
TATGCCTGATCTTACAGGGTGGTCAAAAAATGATGTTATAAAATTAGCTAACATGTTTGATATTGATGTGAAATATAGCG
GGAATGGTTTTGTTTCTGGGCAATCGGTGGCCAAGGGTGGGCCAATAAAATCTGGCATGACCATGACTGTAAGTCTTAAG
AAGTAG

Upstream 100 bases:

>100_bases
AACAGGAAATTAATCAATTGTCTGACCAAAGTCGGTTGAACAAAATTGCTAAAAAGGCAGGCCTTTCTCTCAACTCTGAG
AATATAAGGAATGTAAATAA

Downstream 100 bases:

>100_bases
AGATTAGGAGAGAAAAATGATTAATGGAACAGCTTTAATACTAGGTGCGCTAGTAACATTAGTGGGGATGCCCCAATTTA
TCAATTACTTTAGAGTCCGG

Product: cell division protein FtsI/penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MMNRKKRTTGTTETQHNRKMFGKWLLLIVIGVFIVIVGRFSYIAVSGTVESVNLSSQAKRLYTDNKTIKAKRGSILDTNG
NPIAEDTSTYSVYAVVSKKQVGANNKPLYVTNKKKTAKVLSKYLPISYHKALKTLSPSNSKTFQVEFGNAGSNISVATKE
KIEKEKLNGINFVSSEARLYPNGTFASNLIGFAQQSQQKENDATQLSGVMGIEKMSNKTLTGTDGEQSLQKDKFGYQLPG
SQTERPARDGSTIYTTIDQRLQNIMENVVSKVQSESESQAISATIMNAKTGAILATTQRPTFNASTKKGLSKAWNNALVQ
DTFEPGSTMKIFTTSAAIDSGNFDSDATYRSGTYTIDGKVVPDWKTEGWGVISYAKGFALSSNVAMAHLEQNMGAKTWKK
YINRFKLLQSTNSGLESESKGSIQFDYPIEQANTAFGQAINVTAIQMLQGFTAVANNGKMVKPQFIKKVVDPNTGKTTYK
MKTKDVGRPIKASTAKKVRKLMEDVVYKPYGIGSNFKIKGYKIAAKTGTAQVANASGGYSMGDDSYLYSVAGMAPAKNPK
YIMYITMKKPVLKDKTATQLLDEIFGPVMKQALEQDKETDKSKLDSSIEVPNLVGKDASEAKKELGKKNVAVVTLGNGQT
VKEQSESAGSSILVGQRIILDTGGTITMPDLTGWSKNDVIKLANMFDIDVKYSGNGFVSGQSVAKGGPIKSGMTMTVSLK
K

Sequences:

>Translated_721_residues
MMNRKKRTTGTTETQHNRKMFGKWLLLIVIGVFIVIVGRFSYIAVSGTVESVNLSSQAKRLYTDNKTIKAKRGSILDTNG
NPIAEDTSTYSVYAVVSKKQVGANNKPLYVTNKKKTAKVLSKYLPISYHKALKTLSPSNSKTFQVEFGNAGSNISVATKE
KIEKEKLNGINFVSSEARLYPNGTFASNLIGFAQQSQQKENDATQLSGVMGIEKMSNKTLTGTDGEQSLQKDKFGYQLPG
SQTERPARDGSTIYTTIDQRLQNIMENVVSKVQSESESQAISATIMNAKTGAILATTQRPTFNASTKKGLSKAWNNALVQ
DTFEPGSTMKIFTTSAAIDSGNFDSDATYRSGTYTIDGKVVPDWKTEGWGVISYAKGFALSSNVAMAHLEQNMGAKTWKK
YINRFKLLQSTNSGLESESKGSIQFDYPIEQANTAFGQAINVTAIQMLQGFTAVANNGKMVKPQFIKKVVDPNTGKTTYK
MKTKDVGRPIKASTAKKVRKLMEDVVYKPYGIGSNFKIKGYKIAAKTGTAQVANASGGYSMGDDSYLYSVAGMAPAKNPK
YIMYITMKKPVLKDKTATQLLDEIFGPVMKQALEQDKETDKSKLDSSIEVPNLVGKDASEAKKELGKKNVAVVTLGNGQT
VKEQSESAGSSILVGQRIILDTGGTITMPDLTGWSKNDVIKLANMFDIDVKYSGNGFVSGQSVAKGGPIKSGMTMTVSLK
K
>Mature_721_residues
MMNRKKRTTGTTETQHNRKMFGKWLLLIVIGVFIVIVGRFSYIAVSGTVESVNLSSQAKRLYTDNKTIKAKRGSILDTNG
NPIAEDTSTYSVYAVVSKKQVGANNKPLYVTNKKKTAKVLSKYLPISYHKALKTLSPSNSKTFQVEFGNAGSNISVATKE
KIEKEKLNGINFVSSEARLYPNGTFASNLIGFAQQSQQKENDATQLSGVMGIEKMSNKTLTGTDGEQSLQKDKFGYQLPG
SQTERPARDGSTIYTTIDQRLQNIMENVVSKVQSESESQAISATIMNAKTGAILATTQRPTFNASTKKGLSKAWNNALVQ
DTFEPGSTMKIFTTSAAIDSGNFDSDATYRSGTYTIDGKVVPDWKTEGWGVISYAKGFALSSNVAMAHLEQNMGAKTWKK
YINRFKLLQSTNSGLESESKGSIQFDYPIEQANTAFGQAINVTAIQMLQGFTAVANNGKMVKPQFIKKVVDPNTGKTTYK
MKTKDVGRPIKASTAKKVRKLMEDVVYKPYGIGSNFKIKGYKIAAKTGTAQVANASGGYSMGDDSYLYSVAGMAPAKNPK
YIMYITMKKPVLKDKTATQLLDEIFGPVMKQALEQDKETDKSKLDSSIEVPNLVGKDASEAKKELGKKNVAVVTLGNGQT
VKEQSESAGSSILVGQRIILDTGGTITMPDLTGWSKNDVIKLANMFDIDVKYSGNGFVSGQSVAKGGPIKSGMTMTVSLK
K

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=593, Percent_Identity=25.9696458684654, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI1786854, Length=516, Percent_Identity=22.2868217054264, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 78341; Mature: 78341

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNRKKRTTGTTETQHNRKMFGKWLLLIVIGVFIVIVGRFSYIAVSGTVESVNLSSQAKR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEECCCHHHHHH
LYTDNKTIKAKRGSILDTNGNPIAEDTSTYSVYAVVSKKQVGANNKPLYVTNKKKTAKVL
HHCCCCEEEECCCCEEECCCCCCCCCCCCEEEEEEEEHHHCCCCCCCEEEECCHHHHHHH
SKYLPISYHKALKTLSPSNSKTFQVEFGNAGSNISVATKEKIEKEKLNGINFVSSEARLY
HHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHCCCCEEECCCCEEC
PNGTFASNLIGFAQQSQQKENDATQLSGVMGIEKMSNKTLTGTDGEQSLQKDKFGYQLPG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHCCCEECCC
SQTERPARDGSTIYTTIDQRLQNIMENVVSKVQSESESQAISATIMNAKTGAILATTQRP
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEEECCCCEEEEECCCC
TFNASTKKGLSKAWNNALVQDTFEPGSTMKIFTTSAAIDSGNFDSDATYRSGTYTIDGKV
CCCCHHHHHHHHHHCCCHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCEE
VPDWKTEGWGVISYAKGFALSSNVAMAHLEQNMGAKTWKKYINRFKLLQSTNSGLESESK
CCCCCCCCCEEEEECCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
GSIQFDYPIEQANTAFGQAINVTAIQMLQGFTAVANNGKMVKPQFIKKVVDPNTGKTTYK
CCEEECCCHHHHCHHHCCCHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHCCCCCCEEEE
MKTKDVGRPIKASTAKKVRKLMEDVVYKPYGIGSNFKIKGYKIAAKTGTAQVANASGGYS
EEHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCEEECCCCCCC
MGDDSYLYSVAGMAPAKNPKYIMYITMKKPVLKDKTATQLLDEIFGPVMKQALEQDKETD
CCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KSKLDSSIEVPNLVGKDASEAKKELGKKNVAVVTLGNGQTVKEQSESAGSSILVGQRIIL
HHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCEEEECCEEEE
DTGGTITMPDLTGWSKNDVIKLANMFDIDVKYSGNGFVSGQSVAKGGPIKSGMTMTVSLK
ECCCEEECCCCCCCCCCCEEEEEEEEEEEEEECCCEEECCCCCCCCCCCCCCCEEEEEEC
K
C
>Mature Secondary Structure
MMNRKKRTTGTTETQHNRKMFGKWLLLIVIGVFIVIVGRFSYIAVSGTVESVNLSSQAKR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEECCCHHHHHH
LYTDNKTIKAKRGSILDTNGNPIAEDTSTYSVYAVVSKKQVGANNKPLYVTNKKKTAKVL
HHCCCCEEEECCCCEEECCCCCCCCCCCCEEEEEEEEHHHCCCCCCCEEEECCHHHHHHH
SKYLPISYHKALKTLSPSNSKTFQVEFGNAGSNISVATKEKIEKEKLNGINFVSSEARLY
HHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHCCCCEEECCCCEEC
PNGTFASNLIGFAQQSQQKENDATQLSGVMGIEKMSNKTLTGTDGEQSLQKDKFGYQLPG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHCCCEECCC
SQTERPARDGSTIYTTIDQRLQNIMENVVSKVQSESESQAISATIMNAKTGAILATTQRP
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEEECCCCEEEEECCCC
TFNASTKKGLSKAWNNALVQDTFEPGSTMKIFTTSAAIDSGNFDSDATYRSGTYTIDGKV
CCCCHHHHHHHHHHCCCHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCEE
VPDWKTEGWGVISYAKGFALSSNVAMAHLEQNMGAKTWKKYINRFKLLQSTNSGLESESK
CCCCCCCCCEEEEECCCEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
GSIQFDYPIEQANTAFGQAINVTAIQMLQGFTAVANNGKMVKPQFIKKVVDPNTGKTTYK
CCEEECCCHHHHCHHHCCCHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHCCCCCCEEEE
MKTKDVGRPIKASTAKKVRKLMEDVVYKPYGIGSNFKIKGYKIAAKTGTAQVANASGGYS
EEHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCEEECCCCCCC
MGDDSYLYSVAGMAPAKNPKYIMYITMKKPVLKDKTATQLLDEIFGPVMKQALEQDKETD
CCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KSKLDSSIEVPNLVGKDASEAKKELGKKNVAVVTLGNGQTVKEQSESAGSSILVGQRIIL
HHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCCCEEEECCEEEE
DTGGTITMPDLTGWSKNDVIKLANMFDIDVKYSGNGFVSGQSVAKGGPIKSGMTMTVSLK
ECCCEEECCCCCCCCCCCEEEEEEEEEEEEEECCCEEECCCCCCCCCCCCCCCEEEEEEC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]