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The map label for this gene is rnjA [H]

Identifier: 116492923

GI number: 116492923

Start: 1159195

End: 1160871

Strand: Direct

Name: rnjA [H]

Synonym: PEPE_1162

Alternate gene names: 116492923

Gene position: 1159195-1160871 (Clockwise)

Preceding gene: 116492922

Following gene: 116492954

Centisome position: 63.26

GC content: 38.4

Gene sequence:

>1677_bases
ATGGGTAAAATTAACATTAAAAATAACGAGACTGCCGTTTATGCAATCGGTGGCCTTAATGAAATTGGTAAAAATACTTA
TGGTATTCAATTTCAAGACGAAATTATTGTCGTCGATGCTGGAATCAAATTCCCTGAAGATGAACTATTGGGAATTGACT
ACGTTATCCCTGACTACCAATTTCTAGTTGCTAACCAACAAAAAATAAAAGCCTTAGTTATCACACATGGTCATGAAGAC
CACATTGGTGGTATTCCGTTTTTACTACAACAAATTAACGTTCCAATTTATGCTAGTCCACTTGCTGCCGCTTTAATTAA
AGGAAAACTTGAAGAACATGGTCTATTAAAAACCACCGAAATTCATATCATTGATGAGGATACGGTAATCAAATTTCCTA
AGACTAGCGTTAGCTTCTTTAGAACCACCCACTCGATTCCAGATACTTTAGGGGTTGCCGTCCACACTCCTTCTGGGGTG
ATTGTCGAAACTGGTGATTTTAAGTTTGACCTAACACCAACTACACATCAGCCACCTAACCTTCAAAAAATGGCTAAACT
TGGTGATGAAGGCGTATTGTTGTTAATGTCTGATAGTACTAATGCTGAACGTGATGAATTTACTAAATCTGAACGTTGGG
TTGGTAAGTCCATTCGTAAAATTTTCGAAGAAGTCGAGGGACGAATTATTTTTGCTACTTTCGCTTCTAACATTTCTCGA
ATCCAGATGGCAACAGAAGCTGCACTAAAACATGGTCGTAAAATTGCGGTTTTTGGTCGCTCAATGGAAGCAGCTTTAGT
TAACGGCCGTGAACTAGGATACCTTGATATTCCCGATGACGCATTAATCAATGCCGAACAACTTAACCATTTACCAGCTA
ATAAAGTATTGATCATGTGTACTGGTTCTCAAGGTGAACCGATGGCAGCGCTTAGTCGAATTGCCAACGGCACTCATCGT
CAAATTTCAATACAACCTGATGACACTGTCGTATTTTCAAGTAACCCAATTCCTGGTAATACTATCAGCGTCAATAAAGT
TATTAACGAATTAGAAGAAGCTGGTGCTCGTGTAATTCACGGAAAAGTGAATAACATCCATACTTCTGGTCATGGTGGCC
AAGATGAACAACGTCTAATGTTACGTTTGATGCAACCTAAATATTTTATGCCTATTCATGGCGAATATAGAATGTTGCAA
TTGCATACTGAACTTGCTCATGACTGTGACGTACCATTCGAAAATAGCTTTATTATGTCCAATGGTGATGTTTTAGCCCT
TACAAAGGACTCCGCTCGTCGTTCTGGTCATTTTAGTGCCGGTGATGTATATGTAGATGGAAATGGTATTGGAGATATCG
GAAATGTTGTTTTACGTGATCGTCAATTACTTTCTGAAGAAGGACTTGTGGTTGTCGTTGCAACAATTAACCTAAAAACT
AAGATGATTGAATCCGGTCCAGATCTTTTATCACGAGGATTTGTTTACATGCGAGAATCCGGTGAATTAATTAATGAAGG
TCGGCGTCGAATGTTTGCGAGAATTCGTCACGCAATGCGAAATCCGCACGCTAATGAAGCCTCTATTAATCGGGCAATTA
TTGATGATCTACAGAAATTCTTATATGAACAAACTGAACGTCATCCTGTAATTATTCCAATGTTAATTATGGATTAA

Upstream 100 bases:

>100_bases
AATCCAGACTTCAACGTTGAACAAGTCGAATTGCTCGAAGGTAAGGCACTCGAATATGAACAACAAGGTGTAAATTACAA
GCTAGTGGAGTACTAAGCTT

Downstream 100 bases:

>100_bases
TAAAAAACGCAATCTTATCAAACTCTGAGATAAATCTCTAATGATTGAAAAGATTGCGTTTTTATTTAGCTATAATTTTG
CTAAACATGAAAAGTTCCCT

Product: mRNA degradation ribonuclease J1/J2enzyme

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MGKINIKNNETAVYAIGGLNEIGKNTYGIQFQDEIIVVDAGIKFPEDELLGIDYVIPDYQFLVANQQKIKALVITHGHED
HIGGIPFLLQQINVPIYASPLAAALIKGKLEEHGLLKTTEIHIIDEDTVIKFPKTSVSFFRTTHSIPDTLGVAVHTPSGV
IVETGDFKFDLTPTTHQPPNLQKMAKLGDEGVLLLMSDSTNAERDEFTKSERWVGKSIRKIFEEVEGRIIFATFASNISR
IQMATEAALKHGRKIAVFGRSMEAALVNGRELGYLDIPDDALINAEQLNHLPANKVLIMCTGSQGEPMAALSRIANGTHR
QISIQPDDTVVFSSNPIPGNTISVNKVINELEEAGARVIHGKVNNIHTSGHGGQDEQRLMLRLMQPKYFMPIHGEYRMLQ
LHTELAHDCDVPFENSFIMSNGDVLALTKDSARRSGHFSAGDVYVDGNGIGDIGNVVLRDRQLLSEEGLVVVVATINLKT
KMIESGPDLLSRGFVYMRESGELINEGRRRMFARIRHAMRNPHANEASINRAIIDDLQKFLYEQTERHPVIIPMLIMD

Sequences:

>Translated_558_residues
MGKINIKNNETAVYAIGGLNEIGKNTYGIQFQDEIIVVDAGIKFPEDELLGIDYVIPDYQFLVANQQKIKALVITHGHED
HIGGIPFLLQQINVPIYASPLAAALIKGKLEEHGLLKTTEIHIIDEDTVIKFPKTSVSFFRTTHSIPDTLGVAVHTPSGV
IVETGDFKFDLTPTTHQPPNLQKMAKLGDEGVLLLMSDSTNAERDEFTKSERWVGKSIRKIFEEVEGRIIFATFASNISR
IQMATEAALKHGRKIAVFGRSMEAALVNGRELGYLDIPDDALINAEQLNHLPANKVLIMCTGSQGEPMAALSRIANGTHR
QISIQPDDTVVFSSNPIPGNTISVNKVINELEEAGARVIHGKVNNIHTSGHGGQDEQRLMLRLMQPKYFMPIHGEYRMLQ
LHTELAHDCDVPFENSFIMSNGDVLALTKDSARRSGHFSAGDVYVDGNGIGDIGNVVLRDRQLLSEEGLVVVVATINLKT
KMIESGPDLLSRGFVYMRESGELINEGRRRMFARIRHAMRNPHANEASINRAIIDDLQKFLYEQTERHPVIIPMLIMD
>Mature_557_residues
GKINIKNNETAVYAIGGLNEIGKNTYGIQFQDEIIVVDAGIKFPEDELLGIDYVIPDYQFLVANQQKIKALVITHGHEDH
IGGIPFLLQQINVPIYASPLAAALIKGKLEEHGLLKTTEIHIIDEDTVIKFPKTSVSFFRTTHSIPDTLGVAVHTPSGVI
VETGDFKFDLTPTTHQPPNLQKMAKLGDEGVLLLMSDSTNAERDEFTKSERWVGKSIRKIFEEVEGRIIFATFASNISRI
QMATEAALKHGRKIAVFGRSMEAALVNGRELGYLDIPDDALINAEQLNHLPANKVLIMCTGSQGEPMAALSRIANGTHRQ
ISIQPDDTVVFSSNPIPGNTISVNKVINELEEAGARVIHGKVNNIHTSGHGGQDEQRLMLRLMQPKYFMPIHGEYRMLQL
HTELAHDCDVPFENSFIMSNGDVLALTKDSARRSGHFSAGDVYVDGNGIGDIGNVVLRDRQLLSEEGLVVVVATINLKTK
MIESGPDLLSRGFVYMRESGELINEGRRRMFARIRHAMRNPHANEASINRAIIDDLQKFLYEQTERHPVIIPMLIMD

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61888; Mature: 61757

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKINIKNNETAVYAIGGLNEIGKNTYGIQFQDEIIVVDAGIKFPEDELLGIDYVIPDYQ
CCEEEECCCCEEEEEECCCHHHCCCCCCEEEECCEEEEECCCCCCCHHEEEEEEECCCEE
FLVANQQKIKALVITHGHEDHIGGIPFLLQQINVPIYASPLAAALIKGKLEEHGLLKTTE
EEEECCCEEEEEEEECCCCCCCCCHHHHHHHCCCCEEECHHHHHHHHCCHHHCCCEEEEE
IHIIDEDTVIKFPKTSVSFFRTTHSIPDTLGVAVHTPSGVIVETGDFKFDLTPTTHQPPN
EEEECCCCEEECCCCCHHHHHHHCCCCCCCCEEEECCCCEEEECCCEEEEECCCCCCCCC
LQKMAKLGDEGVLLLMSDSTNAERDEFTKSERWVGKSIRKIFEEVEGRIIFATFASNISR
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHH
IQMATEAALKHGRKIAVFGRSMEAALVNGRELGYLDIPDDALINAEQLNHLPANKVLIMC
HHHHHHHHHHCCCEEEEECCCCEEEEECCCEEEEEECCCHHEECHHHHCCCCCCCEEEEE
TGSQGEPMAALSRIANGTHRQISIQPDDTVVFSSNPIPGNTISVNKVINELEEAGARVIH
ECCCCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCEEEHHHHHHHHHHCCCEEEE
GKVNNIHTSGHGGQDEQRLMLRLMQPKYFMPIHGEYRMLQLHTELAHDCDVPFENSFIMS
ECCCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCEEEEEEEHHHHHHCCCCCCCCEEEE
NGDVLALTKDSARRSGHFSAGDVYVDGNGIGDIGNVVLRDRQLLSEEGLVVVVATINLKT
CCCEEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECEEE
KMIESGPDLLSRGFVYMRESGELINEGRRRMFARIRHAMRNPHANEASINRAIIDDLQKF
EHHHCCHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LYEQTERHPVIIPMLIMD
HHHHCCCCCEEEEEEEEC
>Mature Secondary Structure 
GKINIKNNETAVYAIGGLNEIGKNTYGIQFQDEIIVVDAGIKFPEDELLGIDYVIPDYQ
CEEEECCCCEEEEEECCCHHHCCCCCCEEEECCEEEEECCCCCCCHHEEEEEEECCCEE
FLVANQQKIKALVITHGHEDHIGGIPFLLQQINVPIYASPLAAALIKGKLEEHGLLKTTE
EEEECCCEEEEEEEECCCCCCCCCHHHHHHHCCCCEEECHHHHHHHHCCHHHCCCEEEEE
IHIIDEDTVIKFPKTSVSFFRTTHSIPDTLGVAVHTPSGVIVETGDFKFDLTPTTHQPPN
EEEECCCCEEECCCCCHHHHHHHCCCCCCCCEEEECCCCEEEECCCEEEEECCCCCCCCC
LQKMAKLGDEGVLLLMSDSTNAERDEFTKSERWVGKSIRKIFEEVEGRIIFATFASNISR
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHH
IQMATEAALKHGRKIAVFGRSMEAALVNGRELGYLDIPDDALINAEQLNHLPANKVLIMC
HHHHHHHHHHCCCEEEEECCCCEEEEECCCEEEEEECCCHHEECHHHHCCCCCCCEEEEE
TGSQGEPMAALSRIANGTHRQISIQPDDTVVFSSNPIPGNTISVNKVINELEEAGARVIH
ECCCCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCEEEHHHHHHHHHHCCCEEEE
GKVNNIHTSGHGGQDEQRLMLRLMQPKYFMPIHGEYRMLQLHTELAHDCDVPFENSFIMS
ECCCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCEEEEEEEHHHHHHCCCCCCCCEEEE
NGDVLALTKDSARRSGHFSAGDVYVDGNGIGDIGNVVLRDRQLLSEEGLVVVVATINLKT
CCCEEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECEEE
KMIESGPDLLSRGFVYMRESGELINEGRRRMFARIRHAMRNPHANEASINRAIIDDLQKF
EHHHCCHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LYEQTERHPVIIPMLIMD
HHHHCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]