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The map label for this gene is grpE

Identifier: 116492661

GI number: 116492661

Start: 897397

End: 897969

Strand: Direct

Name: grpE

Synonym: PEPE_0895

Alternate gene names: 116492661

Gene position: 897397-897969 (Clockwise)

Preceding gene: 116492660

Following gene: 116492662

Centisome position: 48.97

GC content: 38.39

Gene sequence:

>573_bases
TTGGCAAAAGAGAAAAAAGAAGAAGTCAAAGAAGAAGAAGTTTCTGAAGCTACTTCAACAGAAGGGTCAACAGATGTTGA
ATCAACTAATAATGATGATTTAACTACAGAAACTCAAGCAACAACGGCTTTAGATGACATTAAAAAGGTGGAAGCTGAAC
GCGATGAACTTTCAGACAAGTATATTCGTGCTCAAGCTGAAATTGTTAATATGCGTCGCCGAAACGAAAAAGAACAGGCA
TCATTAATCAAGTATGATGGTCAAAAACTTGCAAAAGCAATTTTGCCAGCACTTGATAATCTTGAACGCGCTCTAGCAGT
TGAGTCTGCTTCGGAACAACTCTTAAAAGGGGTCAAGATGGTTCAAACCGATTTGTTAAAAGCTTTGAAGGAAAATCACG
TTGCTGAAATTGAAGCTGAAGGACAAGCTTTTGATCCTAATATGCATCAAGCCGTTCAGACGGTGCCAGCTGATGATGAC
CATCCAGCCGATACAGTTGTTCAAGTATTACAAAAAGGATATATTTTAAAAGATCGAGTGTTACGACCAGCAATGGTAAT
CGTAGCTCAATAA

Upstream 100 bases:

>100_bases
TATTCGAAAACATTAGGCTTACTTGATACATTTAGAGATCAGTTGTCTAATCGAATGCTCGAATATTATAAGCACCTTGA
TGATAGTTAGGAGGATTGAA

Downstream 100 bases:

>100_bases
AAAAGAGGAAGAGGGATTTTAAACAATGGCAAGTAATAAAATTATTGGTATTGATTTAGGTACTACAAACTCTGCAGTTG
CTGTCATGGACGGTGACAAA

Product: molecular chaperone GrpE (heat shock protein)

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MAKEKKEEVKEEEVSEATSTEGSTDVESTNNDDLTTETQATTALDDIKKVEAERDELSDKYIRAQAEIVNMRRRNEKEQA
SLIKYDGQKLAKAILPALDNLERALAVESASEQLLKGVKMVQTDLLKALKENHVAEIEAEGQAFDPNMHQAVQTVPADDD
HPADTVVQVLQKGYILKDRVLRPAMVIVAQ

Sequences:

>Translated_190_residues
MAKEKKEEVKEEEVSEATSTEGSTDVESTNNDDLTTETQATTALDDIKKVEAERDELSDKYIRAQAEIVNMRRRNEKEQA
SLIKYDGQKLAKAILPALDNLERALAVESASEQLLKGVKMVQTDLLKALKENHVAEIEAEGQAFDPNMHQAVQTVPADDD
HPADTVVQVLQKGYILKDRVLRPAMVIVAQ
>Mature_189_residues
AKEKKEEVKEEEVSEATSTEGSTDVESTNNDDLTTETQATTALDDIKKVEAERDELSDKYIRAQAEIVNMRRRNEKEQAS
LIKYDGQKLAKAILPALDNLERALAVESASEQLLKGVKMVQTDLLKALKENHVAEIEAEGQAFDPNMHQAVQTVPADDDH
PADTVVQVLQKGYILKDRVLRPAMVIVAQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=160, Percent_Identity=31.875, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1788967, Length=136, Percent_Identity=33.8235294117647, Blast_Score=80, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17552458, Length=151, Percent_Identity=31.7880794701987, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=187, Percent_Identity=31.0160427807487, Blast_Score=81, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24653432, Length=191, Percent_Identity=30.8900523560209, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_PEDPA (Q03FR8)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804396.1
- ProteinModelPortal:   Q03FR8
- SMR:   Q03FR8
- STRING:   Q03FR8
- GeneID:   4417873
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0895
- NMPDR:   fig|278197.10.peg.797
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- BioCyc:   PPEN278197:PEPE_0895-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21128; Mature: 20997

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKKEEVKEEEVSEATSTEGSTDVESTNNDDLTTETQATTALDDIKKVEAERDELSDK
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YIRAQAEIVNMRRRNEKEQASLIKYDGQKLAKAILPALDNLERALAVESASEQLLKGVKM
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQTDLLKALKENHVAEIEAEGQAFDPNMHQAVQTVPADDDHPADTVVQVLQKGYILKDRV
HHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHH
LRPAMVIVAQ
HCCHHHEECC
>Mature Secondary Structure 
AKEKKEEVKEEEVSEATSTEGSTDVESTNNDDLTTETQATTALDDIKKVEAERDELSDK
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YIRAQAEIVNMRRRNEKEQASLIKYDGQKLAKAILPALDNLERALAVESASEQLLKGVKM
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQTDLLKALKENHVAEIEAEGQAFDPNMHQAVQTVPADDDHPADTVVQVLQKGYILKDRV
HHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHH
LRPAMVIVAQ
HCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA