Click here to switch to the map view.

The map label for this gene is truB

Identifier: 116492658

GI number: 116492658

Start: 894391

End: 895290

Strand: Direct

Name: truB

Synonym: PEPE_0892

Alternate gene names: 116492658

Gene position: 894391-895290 (Clockwise)

Preceding gene: 116492657

Following gene: 116492659

Centisome position: 48.81

GC content: 37.78

Gene sequence:

>900_bases
ATGGATGGAATAATTCCAATTAATAAAGAACGAGGAATGACAAGTCACGATGTTGTTGCCAAGGTTCGTGGTATTTTAAG
AACTAAAAAAGTTGGTCATAGTGGAACCCTTGATCCTAGTGTTGACGGGGTTTTACCAATTTGTGTTGGCAGAGCTACGA
AAGTTGTGGACTATTTAATGGGATTTGGAAAAGTTTATCAAGGTTCAATTACGTTAGGTTTCTCGACTACTACGGAAGAT
CTAGATGGTGAAGTAGTTGAAACTAAAAAACTTGATGAACCATTAACGGACGAACAAATTAATCAAACTTTGACTGCAAT
GACAGGAACTTTAATTCAAATTCCACCCATGTATTCCGCAGTAAAGGTTAAGGGCCGCAAGTTATATGAATACGCCCGCG
CTGGTGAGACGGTAGAACGTCCTCAGCGTCAAATTAAGGTTTATGATTTTGTTCAAACTCGCCCATCAATTTTTGATGCA
GAAAAGGGACAACAAACTATTTATTTCAAGGTTAGTTGTGGCAAGGGGACGTATGTGAGAACTTTAGCAGTTGATTTTGG
TCGTCATTTTGGATTACCAGCGGTGATGTCTGATTTAAGTCGCTTGGCTAGTGGGGGATTCAAAATTGAGGAATGCCTGA
CGTTATCCGACTTAGAACAAGCACGAGATGAAGATCGTTTATCGACGGTTATTCAACCTTTAGATCGGGCCTTGGATAAC
TTTAATACAATTACAATTTCAGATGTTGAATGGGAAATTGTTAAAAATGGAGGATTTTTGAATCGTCCGCAAACTGATGA
AATCATAGCTTTAAAATATAATGGGAATATTCAGGCGTTATATCAGTATGATACAAATCGTCAAAACTATATTCCTAAAA
AAATGTTGTTGGTGAGATAG

Upstream 100 bases:

>100_bases
TGGACCTAAATAAATAGGACGAAATCTTTTTAATACGATATAAGTAATGTAAAATGGCTATGAAGAGTGCATTTTAAGTA
CAGAATGAGAGGTTACCAAA

Downstream 100 bases:

>100_bases
AAAATGCAAATAGTAAGAATACATCATCCATATTCAAAAAAAATAATTCCAGCAGAACCTGTAGCACTAGCTTTAGGTTT
CTTTGACGGTGTTCATCGTG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDGIIPINKERGMTSHDVVAKVRGILRTKKVGHSGTLDPSVDGVLPICVGRATKVVDYLMGFGKVYQGSITLGFSTTTED
LDGEVVETKKLDEPLTDEQINQTLTAMTGTLIQIPPMYSAVKVKGRKLYEYARAGETVERPQRQIKVYDFVQTRPSIFDA
EKGQQTIYFKVSCGKGTYVRTLAVDFGRHFGLPAVMSDLSRLASGGFKIEECLTLSDLEQARDEDRLSTVIQPLDRALDN
FNTITISDVEWEIVKNGGFLNRPQTDEIIALKYNGNIQALYQYDTNRQNYIPKKMLLVR

Sequences:

>Translated_299_residues
MDGIIPINKERGMTSHDVVAKVRGILRTKKVGHSGTLDPSVDGVLPICVGRATKVVDYLMGFGKVYQGSITLGFSTTTED
LDGEVVETKKLDEPLTDEQINQTLTAMTGTLIQIPPMYSAVKVKGRKLYEYARAGETVERPQRQIKVYDFVQTRPSIFDA
EKGQQTIYFKVSCGKGTYVRTLAVDFGRHFGLPAVMSDLSRLASGGFKIEECLTLSDLEQARDEDRLSTVIQPLDRALDN
FNTITISDVEWEIVKNGGFLNRPQTDEIIALKYNGNIQALYQYDTNRQNYIPKKMLLVR
>Mature_299_residues
MDGIIPINKERGMTSHDVVAKVRGILRTKKVGHSGTLDPSVDGVLPICVGRATKVVDYLMGFGKVYQGSITLGFSTTTED
LDGEVVETKKLDEPLTDEQINQTLTAMTGTLIQIPPMYSAVKVKGRKLYEYARAGETVERPQRQIKVYDFVQTRPSIFDA
EKGQQTIYFKVSCGKGTYVRTLAVDFGRHFGLPAVMSDLSRLASGGFKIEECLTLSDLEQARDEDRLSTVIQPLDRALDN
FNTITISDVEWEIVKNGGFLNRPQTDEIIALKYNGNIQALYQYDTNRQNYIPKKMLLVR

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=230, Percent_Identity=31.7391304347826, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI215599015, Length=254, Percent_Identity=31.496062992126, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4503337, Length=254, Percent_Identity=31.496062992126, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI2367200, Length=266, Percent_Identity=35.7142857142857, Blast_Score=161, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17553978, Length=254, Percent_Identity=28.740157480315, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323204, Length=270, Percent_Identity=32.962962962963, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6324037, Length=133, Percent_Identity=38.3458646616541, Blast_Score=89, Evalue=7e-19,
Organism=Drosophila melanogaster, GI281364189, Length=269, Percent_Identity=30.4832713754647, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281364187, Length=269, Percent_Identity=30.4832713754647, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281364185, Length=269, Percent_Identity=30.4832713754647, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281364183, Length=269, Percent_Identity=30.4832713754647, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI62471759, Length=269, Percent_Identity=30.4832713754647, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17975520, Length=269, Percent_Identity=30.4832713754647, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_PEDPA (Q03FS1)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804393.1
- ProteinModelPortal:   Q03FS1
- SMR:   Q03FS1
- STRING:   Q03FS1
- GeneID:   4417852
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0892
- NMPDR:   fig|278197.10.peg.794
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q03FS1
- ProtClustDB:   PRK01550
- BioCyc:   PPEN278197:PEPE_0892-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 33454; Mature: 33454

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGIIPINKERGMTSHDVVAKVRGILRTKKVGHSGTLDPSVDGVLPICVGRATKVVDYLM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GFGKVYQGSITLGFSTTTEDLDGEVVETKKLDEPLTDEQINQTLTAMTGTLIQIPPMYSA
HCCCEEECEEEEEECCCCCCCCCCEEEHHHCCCCCCHHHHHHHHHHHHCCEEECCCCCHH
VKVKGRKLYEYARAGETVERPQRQIKVYDFVQTRPSIFDAEKGQQTIYFKVSCGKGTYVR
HHHCCHHHHHHHHCCCHHHHHHHHEEHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEE
TLAVDFGRHFGLPAVMSDLSRLASGGFKIEECLTLSDLEQARDEDRLSTVIQPLDRALDN
EEEEHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FNTITISDVEWEIVKNGGFLNRPQTDEIIALKYNGNIQALYQYDTNRQNYIPKKMLLVR
CCEEEEECCEEEEEECCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHHHEEEC
>Mature Secondary Structure
MDGIIPINKERGMTSHDVVAKVRGILRTKKVGHSGTLDPSVDGVLPICVGRATKVVDYLM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GFGKVYQGSITLGFSTTTEDLDGEVVETKKLDEPLTDEQINQTLTAMTGTLIQIPPMYSA
HCCCEEECEEEEEECCCCCCCCCCEEEHHHCCCCCCHHHHHHHHHHHHCCEEECCCCCHH
VKVKGRKLYEYARAGETVERPQRQIKVYDFVQTRPSIFDAEKGQQTIYFKVSCGKGTYVR
HHHCCHHHHHHHHCCCHHHHHHHHEEHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEE
TLAVDFGRHFGLPAVMSDLSRLASGGFKIEECLTLSDLEQARDEDRLSTVIQPLDRALDN
EEEEHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FNTITISDVEWEIVKNGGFLNRPQTDEIIALKYNGNIQALYQYDTNRQNYIPKKMLLVR
CCEEEEECCEEEEEECCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHHHEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA