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The map label for this gene is pksG [H]

Identifier: 116492634

GI number: 116492634

Start: 870137

End: 871303

Strand: Direct

Name: pksG [H]

Synonym: PEPE_0868

Alternate gene names: 116492634

Gene position: 870137-871303 (Clockwise)

Preceding gene: 116492633

Following gene: 116492637

Centisome position: 47.49

GC content: 35.65

Gene sequence:

>1167_bases
TTGAAAATTGGAATTGATAAGATGAGCTTTTTTACGAGTGATTTATATTTACCGATGGAGGATCTTGCAAAGGCTCGTAA
CGAAGATCCGAATAAATACTTGATTGGTATTGGACAATCAAAACAAGCTGTAATCCGAAATTCACAAGATTCAGTAACAC
TAGCAGCTAATGCAGCATTAAAAATAGTAGATGATAACGAAAAAGAAAAAATTGATTTGATTTTGTTCGGTACTGAATCT
GGTGTAGATAATTCTAAATCAGGAGCAATCTACTTACAGTCATTATTAGGAATTAACTCATCAGCGAGTGGTGTTGAGCT
AAAGCAAGCTTGTTTTGGTTTAACTGCCGGAATTCAAATGGCAATGGGACATATTGCTTTACACCCGGAAAGTCGAGTTT
TACTAGTAGGATCGGATATTGCTCGCTATGGAATTGGTACATTAGGAGAAAAAACTCAAGGTGGAGGAGCAGTCGCGATA
ATTATGAGCGCTAATCCTCATCTCCTTGAAATTGGAACGCAAAGTGCCCATTTAACTAAGGATATTATGGATTTTTGGCG
TCCATTAAATCGTAGTGAAGCGTTAGTTGATGGTAAGTATTCATCAGCCGAATTTATTAATTTCTTCAAAGAAACTTTCG
AAAATTACCTCGAAAAAACGGGCGAAGAGATTGATAAATTTACCGCTCTTATTTTTCATTTACCTTACACTAAGATGGGG
CTTAAAGCTTTAAGAAGCGTAATTGAAGACCTAGATAATGATGATTTAAAAGAAAGTTTATTAGAACATTTTGAGGAGAG
TCGTTATTTGAATCGTGAGGTAGGAAATTTATATACAGGTTCATTGTATCTGAGTCTGTTGTCATTATTACATGCCCGCA
ATTTAAAAGCAGGCGACCAAATTGGCTTGTTTAGTTATGGTTCTGGTGCAGAAGGTGAGTTTTATTCTGCTAAAGTCGTT
GAAGGATATGAAAAGTATGTTACAGATGAAGAAATTGCATTTTTGGATCAAAGAACTTCGGTCTCAGTGGCTGAATATGA
ACAAATCTATCGTAGTTTTATGAGACCTCAAACAGCGGACTACAGAACTGAATTTGAAAACGATCCTGCACCATTTGTTT
TGAAGGGACAACAACAACAACAACGGATTTATGAAAAAGTAAATTAA

Upstream 100 bases:

>100_bases
AAGGTTTACCTAAGAAATAAGCTGAACTTTGATAAACAGATAGGGGTCTGCTAAAATTTAATAGATAAAAAATTTAGGAT
TAAGCTTTGGAGGATTGGTT

Downstream 100 bases:

>100_bases
AAAAATGGCAACTTGGGTTTAAACCTAAGTTGCCATTTTTTATGAGAAGATGCTGTAAACAATTTTACCGTCTTTCGCGA
TAATCATCGCTTTATTTTTG

Product: hydroxymethylglutaryl-CoA synthase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKIGIDKMSFFTSDLYLPMEDLAKARNEDPNKYLIGIGQSKQAVIRNSQDSVTLAANAALKIVDDNEKEKIDLILFGTES
GVDNSKSGAIYLQSLLGINSSASGVELKQACFGLTAGIQMAMGHIALHPESRVLLVGSDIARYGIGTLGEKTQGGGAVAI
IMSANPHLLEIGTQSAHLTKDIMDFWRPLNRSEALVDGKYSSAEFINFFKETFENYLEKTGEEIDKFTALIFHLPYTKMG
LKALRSVIEDLDNDDLKESLLEHFEESRYLNREVGNLYTGSLYLSLLSLLHARNLKAGDQIGLFSYGSGAEGEFYSAKVV
EGYEKYVTDEEIAFLDQRTSVSVAEYEQIYRSFMRPQTADYRTEFENDPAPFVLKGQQQQQRIYEKVN

Sequences:

>Translated_388_residues
MKIGIDKMSFFTSDLYLPMEDLAKARNEDPNKYLIGIGQSKQAVIRNSQDSVTLAANAALKIVDDNEKEKIDLILFGTES
GVDNSKSGAIYLQSLLGINSSASGVELKQACFGLTAGIQMAMGHIALHPESRVLLVGSDIARYGIGTLGEKTQGGGAVAI
IMSANPHLLEIGTQSAHLTKDIMDFWRPLNRSEALVDGKYSSAEFINFFKETFENYLEKTGEEIDKFTALIFHLPYTKMG
LKALRSVIEDLDNDDLKESLLEHFEESRYLNREVGNLYTGSLYLSLLSLLHARNLKAGDQIGLFSYGSGAEGEFYSAKVV
EGYEKYVTDEEIAFLDQRTSVSVAEYEQIYRSFMRPQTADYRTEFENDPAPFVLKGQQQQQRIYEKVN
>Mature_388_residues
MKIGIDKMSFFTSDLYLPMEDLAKARNEDPNKYLIGIGQSKQAVIRNSQDSVTLAANAALKIVDDNEKEKIDLILFGTES
GVDNSKSGAIYLQSLLGINSSASGVELKQACFGLTAGIQMAMGHIALHPESRVLLVGSDIARYGIGTLGEKTQGGGAVAI
IMSANPHLLEIGTQSAHLTKDIMDFWRPLNRSEALVDGKYSSAEFINFFKETFENYLEKTGEEIDKFTALIFHLPYTKMG
LKALRSVIEDLDNDDLKESLLEHFEESRYLNREVGNLYTGSLYLSLLSLLHARNLKAGDQIGLFSYGSGAEGEFYSAKVV
EGYEKYVTDEEIAFLDQRTSVSVAEYEQIYRSFMRPQTADYRTEFENDPAPFVLKGQQQQQRIYEKVN

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI148298764, Length=376, Percent_Identity=29.7872340425532, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI148298677, Length=376, Percent_Identity=29.7872340425532, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI5031751, Length=376, Percent_Identity=27.6595744680851, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI260656028, Length=373, Percent_Identity=23.5924932975871, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17560126, Length=419, Percent_Identity=25.7756563245823, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323509, Length=400, Percent_Identity=24, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24654139, Length=409, Percent_Identity=27.3838630806846, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24654137, Length=409, Percent_Identity=27.3838630806846, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24654133, Length=409, Percent_Identity=27.3838630806846, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24654135, Length=409, Percent_Identity=27.3838630806846, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17933694, Length=409, Percent_Identity=27.3838630806846, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 43265; Mature: 43265

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIDKMSFFTSDLYLPMEDLAKARNEDPNKYLIGIGQSKQAVIRNSQDSVTLAANAAL
CCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCCCEEEEECCEE
KIVDDNEKEKIDLILFGTESGVDNSKSGAIYLQSLLGINSSASGVELKQACFGLTAGIQM
EEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
AMGHIALHPESRVLLVGSDIARYGIGTLGEKTQGGGAVAIIMSANPHLLEIGTQSAHLTK
HHHHEEECCCCCEEEEECCHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEECCCHHHHHH
DIMDFWRPLNRSEALVDGKYSSAEFINFFKETFENYLEKTGEEIDKFTALIFHLPYTKMG
HHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LKALRSVIEDLDNDDLKESLLEHFEESRYLNREVGNLYTGSLYLSLLSLLHARNLKAGDQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHCCCCCCCC
IGLFSYGSGAEGEFYSAKVVEGYEKYVTDEEIAFLDQRTSVSVAEYEQIYRSFMRPQTAD
EEEEECCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
YRTEFENDPAPFVLKGQQQQQRIYEKVN
HHHHCCCCCCCEEEECCHHHHHHHHHCC
>Mature Secondary Structure
MKIGIDKMSFFTSDLYLPMEDLAKARNEDPNKYLIGIGQSKQAVIRNSQDSVTLAANAAL
CCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCCCEEEEECCEE
KIVDDNEKEKIDLILFGTESGVDNSKSGAIYLQSLLGINSSASGVELKQACFGLTAGIQM
EEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
AMGHIALHPESRVLLVGSDIARYGIGTLGEKTQGGGAVAIIMSANPHLLEIGTQSAHLTK
HHHHEEECCCCCEEEEECCHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEECCCHHHHHH
DIMDFWRPLNRSEALVDGKYSSAEFINFFKETFENYLEKTGEEIDKFTALIFHLPYTKMG
HHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LKALRSVIEDLDNDDLKESLLEHFEESRYLNREVGNLYTGSLYLSLLSLLHARNLKAGDQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHCCCCCCCC
IGLFSYGSGAEGEFYSAKVVEGYEKYVTDEEIAFLDQRTSVSVAEYEQIYRSFMRPQTAD
EEEEECCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
YRTEFENDPAPFVLKGQQQQQRIYEKVN
HHHHCCCCCCCEEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]