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The map label for this gene is ffh [H]

Identifier: 116492613

GI number: 116492613

Start: 853586

End: 855016

Strand: Direct

Name: ffh [H]

Synonym: PEPE_0846

Alternate gene names: 116492613

Gene position: 853586-855016 (Clockwise)

Preceding gene: 116492612

Following gene: 116492614

Centisome position: 46.58

GC content: 38.43

Gene sequence:

>1431_bases
ATGGCTTTTGAGGGTCTAACTGAACGATTACAAAAAACTTTCACTAATTTAAGAAAAAAGGGTAAAGTTACTGAGGCTGA
TTTACGCGATACAATGCGTGAAATTCGCTTAGCGTTGCTAGAAGCCGATGTTAACTTTAAAGTAGTTAAACAATTTGTTA
AAACCGTCCGAGACCAAGCAATTGGCTCAGATATTTTAGATGGATTAAATCCTGCTCAGCAAATTGTGAAGTTGGTTAAT
GATGAATTAATCAAGATGATGGGGACAGAAGATGCACCATTAAATAAATCTCCCAAAATTCCAACAATTATTATGATGTC
TGGTTTACAAGGGGCTGGTAAAACAACTACCACTGGTAAATTAGCATTAAAACTTAAAAATGAACAAAAAGCTCGTCCAC
TTTTGATTGCGGCCGATGTTTATCGTCCAGCGGCAATTGATCAGTTGGTTCAAGTCGGGCAACAAATAGACGTTCCGGTT
TACCAAGAAGGCACAGATGTTAACCCCGTTGATATTGTGCGTCATGGTTTGGAACAAGCTGCTGAAAATAAAAACGATTA
TGTCTTTATTGATACAGCTGGTCGTCTACAAATTGACGAAAAATTAATGCAGGAATTGGCTGATATTAAAGCGTTAGCTC
ATCCGGATGAAATTCTCCTGGTTGTTGATGCAATGACTGGTCAAAACGCCGTTAATACTGCGGAAGGATTTAATGAAAAA
TTAGATATTACTGGGGTAATTCTAACTAAGTTAGATGGCGATACCCGTGGTGGTGCTGCGTTGTCAATTCGCTCAGTCAC
TGGTAAACCAATTAAATTTGTTGGTCAAGGCGAAAAGATGGAAGATTTGGATGTTTTCCATCCTGATCGAATGGCTTCCC
GTATTTTAGGTATGGGTGACATGTTAACTTTGATCGAAAAAACTCAAAAAGATTTCGACCAAAAGCAAGCAGCTGAAATG
ACTCAAAAGATTCGCGAAAACACATTCGACTTTAACGATTTCGTGGATCAATTGAATCAAGTTCAAAATATGGGACCAAT
TGAGGATATCATGAAAATGATTCCAGGTATGGCTAATAACCCAGCTATGCAAAATGTGAATGTGGACCCTAAGGATCTTG
AGCACACCAAGGCGATTGTATATTCCATGACCAAAGCTGAGCGTGAAAATCCAGATCTTCTAAATCCTAGTCGCCGTCGT
CGGATTGCGGCTGGATCCGGGCGTCCTGTGGTTGAAGTCAATCGGATGATTAAACAATTTAATCAAATGAAGAAAATGAT
GAACCAAATGTCAAAAGGAAACTTTAACGGCATGGAAGGTATGTTAGGAAATGGAATTAAGGGTAAGTTGTCGAAGATGG
CAATGAATTCCATGATTAAGAAAAATAAGAAAAACAAAAAGAAGCGGATGAAGAAAAATAAACGACGCTAA

Upstream 100 bases:

>100_bases
GTGCTTATATTAATCAAAAATATCCGGAAGATACTGAATTAAAACACTTTTTAGATGATCTCGAAAAAAGTGAAGAAGAA
TAAAGACGGAGGGGCTTTAA

Downstream 100 bases:

>100_bases
AAATTAAGAGGTTTGGTTATACAACTAAACCTCTTTTTGTTTTCTAATAAAAGGTACTAGGATTTCTACTAAAAATATGG
AAATAGTACCCCAGAAAAAT

Product: signal recognition particle subunit FFH/SRP54 (srp54)

Products: NA

Alternate protein names: Fifty-four homolog [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAFEGLTERLQKTFTNLRKKGKVTEADLRDTMREIRLALLEADVNFKVVKQFVKTVRDQAIGSDILDGLNPAQQIVKLVN
DELIKMMGTEDAPLNKSPKIPTIIMMSGLQGAGKTTTTGKLALKLKNEQKARPLLIAADVYRPAAIDQLVQVGQQIDVPV
YQEGTDVNPVDIVRHGLEQAAENKNDYVFIDTAGRLQIDEKLMQELADIKALAHPDEILLVVDAMTGQNAVNTAEGFNEK
LDITGVILTKLDGDTRGGAALSIRSVTGKPIKFVGQGEKMEDLDVFHPDRMASRILGMGDMLTLIEKTQKDFDQKQAAEM
TQKIRENTFDFNDFVDQLNQVQNMGPIEDIMKMIPGMANNPAMQNVNVDPKDLEHTKAIVYSMTKAERENPDLLNPSRRR
RIAAGSGRPVVEVNRMIKQFNQMKKMMNQMSKGNFNGMEGMLGNGIKGKLSKMAMNSMIKKNKKNKKKRMKKNKRR

Sequences:

>Translated_476_residues
MAFEGLTERLQKTFTNLRKKGKVTEADLRDTMREIRLALLEADVNFKVVKQFVKTVRDQAIGSDILDGLNPAQQIVKLVN
DELIKMMGTEDAPLNKSPKIPTIIMMSGLQGAGKTTTTGKLALKLKNEQKARPLLIAADVYRPAAIDQLVQVGQQIDVPV
YQEGTDVNPVDIVRHGLEQAAENKNDYVFIDTAGRLQIDEKLMQELADIKALAHPDEILLVVDAMTGQNAVNTAEGFNEK
LDITGVILTKLDGDTRGGAALSIRSVTGKPIKFVGQGEKMEDLDVFHPDRMASRILGMGDMLTLIEKTQKDFDQKQAAEM
TQKIRENTFDFNDFVDQLNQVQNMGPIEDIMKMIPGMANNPAMQNVNVDPKDLEHTKAIVYSMTKAERENPDLLNPSRRR
RIAAGSGRPVVEVNRMIKQFNQMKKMMNQMSKGNFNGMEGMLGNGIKGKLSKMAMNSMIKKNKKNKKKRMKKNKRR
>Mature_475_residues
AFEGLTERLQKTFTNLRKKGKVTEADLRDTMREIRLALLEADVNFKVVKQFVKTVRDQAIGSDILDGLNPAQQIVKLVND
ELIKMMGTEDAPLNKSPKIPTIIMMSGLQGAGKTTTTGKLALKLKNEQKARPLLIAADVYRPAAIDQLVQVGQQIDVPVY
QEGTDVNPVDIVRHGLEQAAENKNDYVFIDTAGRLQIDEKLMQELADIKALAHPDEILLVVDAMTGQNAVNTAEGFNEKL
DITGVILTKLDGDTRGGAALSIRSVTGKPIKFVGQGEKMEDLDVFHPDRMASRILGMGDMLTLIEKTQKDFDQKQAAEMT
QKIRENTFDFNDFVDQLNQVQNMGPIEDIMKMIPGMANNPAMQNVNVDPKDLEHTKAIVYSMTKAERENPDLLNPSRRRR
IAAGSGRPVVEVNRMIKQFNQMKKMMNQMSKGNFNGMEGMLGNGIKGKLSKMAMNSMIKKNKKNKKKRMKKNKRR

Specific function: Necessary for efficient export of extracytoplasmic proteins. Binds to the signal sequence when it emerges from the ribosomes [H]

COG id: COG0541

COG function: function code U; Signal recognition particle GTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding SRP family [H]

Homologues:

Organism=Homo sapiens, GI4507215, Length=446, Percent_Identity=32.0627802690583, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI226371618, Length=383, Percent_Identity=32.3759791122715, Blast_Score=202, Evalue=8e-52,
Organism=Homo sapiens, GI89061728, Length=146, Percent_Identity=36.986301369863, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI23308697, Length=207, Percent_Identity=31.4009661835749, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI295424842, Length=207, Percent_Identity=31.4009661835749, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788963, Length=452, Percent_Identity=46.9026548672566, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1789874, Length=267, Percent_Identity=35.9550561797753, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17539958, Length=448, Percent_Identity=29.9107142857143, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI193206261, Length=287, Percent_Identity=29.9651567944251, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6325345, Length=408, Percent_Identity=34.0686274509804, Blast_Score=230, Evalue=3e-61,
Organism=Drosophila melanogaster, GI17647949, Length=451, Percent_Identity=31.4855875831486, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24641198, Length=289, Percent_Identity=27.681660899654, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR000897
- InterPro:   IPR013822
- InterPro:   IPR004780
- InterPro:   IPR004125 [H]

Pfam domain/function: PF00448 SRP54; PF02881 SRP54_N; PF02978 SRP_SPB [H]

EC number: NA

Molecular weight: Translated: 53211; Mature: 53080

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEGLTERLQKTFTNLRKKGKVTEADLRDTMREIRLALLEADVNFKVVKQFVKTVRDQA
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IGSDILDGLNPAQQIVKLVNDELIKMMGTEDAPLNKSPKIPTIIMMSGLQGAGKTTTTGK
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCE
LALKLKNEQKARPLLIAADVYRPAAIDQLVQVGQQIDVPVYQEGTDVNPVDIVRHGLEQA
EEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
AENKNDYVFIDTAGRLQIDEKLMQELADIKALAHPDEILLVVDAMTGQNAVNTAEGFNEK
HCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCCCC
LDITGVILTKLDGDTRGGAALSIRSVTGKPIKFVGQGEKMEDLDVFHPDRMASRILGMGD
CCEEEEEEEEECCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCHH
MLTLIEKTQKDFDQKQAAEMTQKIRENTFDFNDFVDQLNQVQNMGPIEDIMKMIPGMANN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
PAMQNVNVDPKDLEHTKAIVYSMTKAERENPDLLNPSRRRRIAAGSGRPVVEVNRMIKQF
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEECCCCCCCHHHHHHHHHHH
NQMKKMMNQMSKGNFNGMEGMLGNGIKGKLSKMAMNSMIKKNKKNKKKRMKKNKRR
HHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AFEGLTERLQKTFTNLRKKGKVTEADLRDTMREIRLALLEADVNFKVVKQFVKTVRDQA
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IGSDILDGLNPAQQIVKLVNDELIKMMGTEDAPLNKSPKIPTIIMMSGLQGAGKTTTTGK
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCE
LALKLKNEQKARPLLIAADVYRPAAIDQLVQVGQQIDVPVYQEGTDVNPVDIVRHGLEQA
EEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
AENKNDYVFIDTAGRLQIDEKLMQELADIKALAHPDEILLVVDAMTGQNAVNTAEGFNEK
HCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCCCC
LDITGVILTKLDGDTRGGAALSIRSVTGKPIKFVGQGEKMEDLDVFHPDRMASRILGMGD
CCEEEEEEEEECCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCHH
MLTLIEKTQKDFDQKQAAEMTQKIRENTFDFNDFVDQLNQVQNMGPIEDIMKMIPGMANN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
PAMQNVNVDPKDLEHTKAIVYSMTKAERENPDLLNPSRRRRIAAGSGRPVVEVNRMIKQF
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEECCCCCCCHHHHHHHHHHH
NQMKKMMNQMSKGNFNGMEGMLGNGIKGKLSKMAMNSMIKKNKKNKKKRMKKNKRR
HHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12397186; 8763945 [H]