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The map label for this gene is miaA

Identifier: 116492504

GI number: 116492504

Start: 773109

End: 774041

Strand: Direct

Name: miaA

Synonym: PEPE_0736

Alternate gene names: 116492504

Gene position: 773109-774041 (Clockwise)

Preceding gene: 116492503

Following gene: 116492505

Centisome position: 42.19

GC content: 37.08

Gene sequence:

>933_bases
ATGGAAAAAGTTTTAGCGATTGTTGGTCCAACTGCAGTCGGAAAAACCAACCTGTCGATTGATATTGCAAAAAAATTTAA
CGGAGAGATCATTTCTGGGGATTCGATGCAAGTCTATCAAAAATTAGATATTGGCACGGCAAAAGTTACCATAGAGGAAA
TGCAAGGAATCCCACACCATTTGATTGATACCAAGACTATCCATGATCGCTTTTCAGCTGCTGATTTTAAAGAAACGGCT
CAAAAATTGATTAGTGAAATTAGTCAGCGAGGTAAGCTACCAATTGTGGTTGGTGGAACCGGTTTTTACCTACAGGCTCT
ATTAAGAGATTTGGAGTTAGGAGGTAGCAATGAAGATCGAAATCAAATTGAAGCGATCCGTACAGAGTTAAATCAAAAAG
AAAATGATGAACTATTATCGATACTAAAAACAATCGATTTAGCAACTTACGAAAATATTGATCGAAAGAACCGGCGACGA
ATTATTCGAGCAATTGAAGTTTTTAAAGTGTCAGGCCAAAAGATGTCAGAACAGCAAATGCAACCGACGGCGGTTTATGA
TGACCTACTAATTGGCTTAAATACGGAACGCTCTATCTTATACCAACGTATCAACCATCGGGTAAACATGATGTTAGACG
CTGGACTTGAAAATGAAGCACGCTGGTTGTTTGACGAAACTAAGGTACCACAAGCCAACAAGGGGATTGGATATCGAGAG
TGGCAGGATTACTTTAAAAATGAGAAAACTTTATCAGAAACCATTGAGCTAATACAAAAGGATTCTAGACACTATGCTAA
ACGTCAACTTACATGGTTTAGAAATAAGATGGATGTAAATTGGTATGACATTATCCAAAATCCACAAGAGCAAGCAAAGA
TATATCAAAAAATTGAAAACTGGTTAGGTGTTAAAAATGAATTGGAAAGATGA

Upstream 100 bases:

>100_bases
GATGATATCAAGTACTGCTTTAAAAAGAATTTTAAGGGGATTATCACGGACTATCCAACAAGAGCTTTAAAAATTAGAAA
AAAGATGCAGGGAGAATAAT

Downstream 100 bases:

>100_bases
TTTTAAACCGGAAGTAAAAAGAATCATTGAAGAGGTTGAAAAACAAATTCAACCACGACTAAGTGAGATTGATGATCAAG
TAACTTTTAATCAACGGAAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MEKVLAIVGPTAVGKTNLSIDIAKKFNGEIISGDSMQVYQKLDIGTAKVTIEEMQGIPHHLIDTKTIHDRFSAADFKETA
QKLISEISQRGKLPIVVGGTGFYLQALLRDLELGGSNEDRNQIEAIRTELNQKENDELLSILKTIDLATYENIDRKNRRR
IIRAIEVFKVSGQKMSEQQMQPTAVYDDLLIGLNTERSILYQRINHRVNMMLDAGLENEARWLFDETKVPQANKGIGYRE
WQDYFKNEKTLSETIELIQKDSRHYAKRQLTWFRNKMDVNWYDIIQNPQEQAKIYQKIENWLGVKNELER

Sequences:

>Translated_310_residues
MEKVLAIVGPTAVGKTNLSIDIAKKFNGEIISGDSMQVYQKLDIGTAKVTIEEMQGIPHHLIDTKTIHDRFSAADFKETA
QKLISEISQRGKLPIVVGGTGFYLQALLRDLELGGSNEDRNQIEAIRTELNQKENDELLSILKTIDLATYENIDRKNRRR
IIRAIEVFKVSGQKMSEQQMQPTAVYDDLLIGLNTERSILYQRINHRVNMMLDAGLENEARWLFDETKVPQANKGIGYRE
WQDYFKNEKTLSETIELIQKDSRHYAKRQLTWFRNKMDVNWYDIIQNPQEQAKIYQKIENWLGVKNELER
>Mature_310_residues
MEKVLAIVGPTAVGKTNLSIDIAKKFNGEIISGDSMQVYQKLDIGTAKVTIEEMQGIPHHLIDTKTIHDRFSAADFKETA
QKLISEISQRGKLPIVVGGTGFYLQALLRDLELGGSNEDRNQIEAIRTELNQKENDELLSILKTIDLATYENIDRKNRRR
IIRAIEVFKVSGQKMSEQQMQPTAVYDDLLIGLNTERSILYQRINHRVNMMLDAGLENEARWLFDETKVPQANKGIGYRE
WQDYFKNEKTLSETIELIQKDSRHYAKRQLTWFRNKMDVNWYDIIQNPQEQAKIYQKIENWLGVKNELER

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=309, Percent_Identity=27.1844660194175, Blast_Score=125, Evalue=3e-29,
Organism=Escherichia coli, GI1790613, Length=311, Percent_Identity=32.1543408360129, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25144712, Length=246, Percent_Identity=32.1138211382114, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=32.8767123287671, Blast_Score=148, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24649830, Length=331, Percent_Identity=32.9305135951662, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PEDPA (Q03G75)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804239.1
- ProteinModelPortal:   Q03G75
- SMR:   Q03G75
- STRING:   Q03G75
- GeneID:   4418061
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0736
- NMPDR:   fig|278197.10.peg.657
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q03G75
- BioCyc:   PPEN278197:PEPE_0736-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35931; Mature: 35931

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVLAIVGPTAVGKTNLSIDIAKKFNGEIISGDSMQVYQKLDIGTAKVTIEEMQGIPHH
CCCEEEEECCCCCCCCCCEEEEEHHCCCCEECCCHHHHHHHHCCCHHEEEHHHHCCCCHH
LIDTKTIHDRFSAADFKETAQKLISEISQRGKLPIVVGGTGFYLQALLRDLELGGSNEDR
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCHH
NQIEAIRTELNQKENDELLSILKTIDLATYENIDRKNRRRIIRAIEVFKVSGQKMSEQQM
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHC
QPTAVYDDLLIGLNTERSILYQRINHRVNMMLDAGLENEARWLFDETKVPQANKGIGYRE
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHCCCCCCCCCCCCCHHH
WQDYFKNEKTLSETIELIQKDSRHYAKRQLTWFRNKMDVNWYDIIQNPQEQAKIYQKIEN
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHH
WLGVKNELER
HHCCHHHCCC
>Mature Secondary Structure
MEKVLAIVGPTAVGKTNLSIDIAKKFNGEIISGDSMQVYQKLDIGTAKVTIEEMQGIPHH
CCCEEEEECCCCCCCCCCEEEEEHHCCCCEECCCHHHHHHHHCCCHHEEEHHHHCCCCHH
LIDTKTIHDRFSAADFKETAQKLISEISQRGKLPIVVGGTGFYLQALLRDLELGGSNEDR
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCHH
NQIEAIRTELNQKENDELLSILKTIDLATYENIDRKNRRRIIRAIEVFKVSGQKMSEQQM
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHC
QPTAVYDDLLIGLNTERSILYQRINHRVNMMLDAGLENEARWLFDETKVPQANKGIGYRE
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHCCCCCCCCCCCCCHHH
WQDYFKNEKTLSETIELIQKDSRHYAKRQLTWFRNKMDVNWYDIIQNPQEQAKIYQKIEN
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHH
WLGVKNELER
HHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA