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The map label for this gene is trmB

Identifier: 116492450

GI number: 116492450

Start: 722332

End: 722970

Strand: Direct

Name: trmB

Synonym: PEPE_0682

Alternate gene names: 116492450

Gene position: 722332-722970 (Clockwise)

Preceding gene: 116492449

Following gene: 116492452

Centisome position: 39.42

GC content: 34.43

Gene sequence:

>639_bases
ATGCGAGTTAGAAATAAACCATGGGCACCGGAGTTAATCGAGGCTCATCCAGAAAAGATTGTTGAAAAGGGCCAAGCGTT
CAAGGGTCAATGGAACCAGCGGTTTGAAAAAGAACAACCAATTTTTATTGAAGTAGGAACGGGTAAGGGTCAATTCATTA
TTAATATGGCCAAAAAATATCCTCAATACAATTTTATTGGAATTGAAATTCAAAAGACTGTAATTGCCATTGCTTTAAAA
AATGCTTTGGAGGAAGAAATTCCCAATTTGCAATTCTTATATGCTGATGGCGCTGAACTTACGGATTATTTTGAAGATGG
TGAGGTTTCCAAAGTTTTCCTTAACTTTTCAGACCCATGGCCTAAAACACGTCATGAAAAACGCCGGTTAACCTTTAAGA
CTTTCTTGAAAGTTTATGAACAAATTTTAGTTAAAAATGGGGAAGTTGAATTTAAAACGGATAATCAAGGATTATTCGAA
TATTCACTTTACAGCCTTAATAACTATGGAATGACTTTTGAAGGCGTTTGGTTAGACTTACACAATAGTGAAGAAAATGA
AGATAACGTTGAAACTGAATATGAACATAAATTCAGTGCTAAAGGCCAGCCAATTTACAAATTAAAAGCACATTTCTAA

Upstream 100 bases:

>100_bases
AGTTGCATCGGTGGTAACACAGGGGATTGTTGCAGTGATTTTACTCAAGTGGTTCATACCACGTTATATAAAAAAATTAA
ATTAAATTTAAGAGGTAATT

Downstream 100 bases:

>100_bases
AGTGAAAAATAAAAAGAGTCTGAGGATTTTTCCTCAGACTCTCTATTAGTTTGCTATTATCGACGATAAATATCGATAAT
GGTACCAGTATAGGCATCTG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MRVRNKPWAPELIEAHPEKIVEKGQAFKGQWNQRFEKEQPIFIEVGTGKGQFIINMAKKYPQYNFIGIEIQKTVIAIALK
NALEEEIPNLQFLYADGAELTDYFEDGEVSKVFLNFSDPWPKTRHEKRRLTFKTFLKVYEQILVKNGEVEFKTDNQGLFE
YSLYSLNNYGMTFEGVWLDLHNSEENEDNVETEYEHKFSAKGQPIYKLKAHF

Sequences:

>Translated_212_residues
MRVRNKPWAPELIEAHPEKIVEKGQAFKGQWNQRFEKEQPIFIEVGTGKGQFIINMAKKYPQYNFIGIEIQKTVIAIALK
NALEEEIPNLQFLYADGAELTDYFEDGEVSKVFLNFSDPWPKTRHEKRRLTFKTFLKVYEQILVKNGEVEFKTDNQGLFE
YSLYSLNNYGMTFEGVWLDLHNSEENEDNVETEYEHKFSAKGQPIYKLKAHF
>Mature_212_residues
MRVRNKPWAPELIEAHPEKIVEKGQAFKGQWNQRFEKEQPIFIEVGTGKGQFIINMAKKYPQYNFIGIEIQKTVIAIALK
NALEEEIPNLQFLYADGAELTDYFEDGEVSKVFLNFSDPWPKTRHEKRRLTFKTFLKVYEQILVKNGEVEFKTDNQGLFE
YSLYSLNNYGMTFEGVWLDLHNSEENEDNVETEYEHKFSAKGQPIYKLKAHF

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=135, Percent_Identity=32.5925925925926, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_PEDPA (Q03GC9)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804185.1
- ProteinModelPortal:   Q03GC9
- SMR:   Q03GC9
- STRING:   Q03GC9
- GeneID:   4418147
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0682
- NMPDR:   fig|278197.10.peg.607
- eggNOG:   COG0220
- HOGENOM:   HBG304014
- OMA:   LRNKPWA
- PhylomeDB:   Q03GC9
- ProtClustDB:   PRK00121
- BioCyc:   PPEN278197:PEPE_0682-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 24943; Mature: 24943

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 69-69 BINDING 96-96 BINDING 118-118 BINDING 122-122 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRNKPWAPELIEAHPEKIVEKGQAFKGQWNQRFEKEQPIFIEVGTGKGQFIINMAKKY
CCCCCCCCCCHHHHCCHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCCEEEEEEHHHC
PQYNFIGIEIQKTVIAIALKNALEEEIPNLQFLYADGAELTDYFEDGEVSKVFLNFSDPW
CCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCEEEEEEECCCCC
PKTRHEKRRLTFKTFLKVYEQILVKNGEVEFKTDNQGLFEYSLYSLNNYGMTFEGVWLDL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEECCCCCEEEEEEEEE
HNSEENEDNVETEYEHKFSAKGQPIYKLKAHF
CCCCCCCCCCCCHHHHHCCCCCCEEEEEEECC
>Mature Secondary Structure
MRVRNKPWAPELIEAHPEKIVEKGQAFKGQWNQRFEKEQPIFIEVGTGKGQFIINMAKKY
CCCCCCCCCCHHHHCCHHHHHHCCCCCCCCHHHHHCCCCCEEEEEECCCCEEEEEEHHHC
PQYNFIGIEIQKTVIAIALKNALEEEIPNLQFLYADGAELTDYFEDGEVSKVFLNFSDPW
CCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCEEEEEEECCCCC
PKTRHEKRRLTFKTFLKVYEQILVKNGEVEFKTDNQGLFEYSLYSLNNYGMTFEGVWLDL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEEEECCCCCEEEEEEEEE
HNSEENEDNVETEYEHKFSAKGQPIYKLKAHF
CCCCCCCCCCCCHHHHHCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA