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The map label for this gene is ytpR [H]

Identifier: 116492452

GI number: 116492452

Start: 723436

End: 724068

Strand: Direct

Name: ytpR [H]

Synonym: PEPE_0684

Alternate gene names: 116492452

Gene position: 723436-724068 (Clockwise)

Preceding gene: 116492450

Following gene: 116492453

Centisome position: 39.48

GC content: 36.18

Gene sequence:

>633_bases
ATGTTAATTGCTAGTTATAATAAAGAAACCATGGGAGATATCCTAATTACGATGATTCGTCCAGATGCTGAGGAAGCAAA
AATTGAACAAAAAGGCTCCGTAGTTCGAATTGCCGATGCTAAAACAAATGAAACCACCGGTTTTAATTTCTTAAATGTTA
GCGAAATTTTTGAAATCAATGGTAAGGGTCCCATTACTTTGACTCAAGAACAAGTAAAGCAACTTAATGCCACATTATCT
GCTAATGGTTTTGAAGCTGAATTAGTAGCAGATGAACGTCCAAAATTTGTCGTGGGTTACGTGAAATCAACTAAACCACA
TCCAGATTCAGATCATTTGTTAATTACTGAAACTGAGGTGGATAATGGTGAAACTTTGCAAATTGTTAGTGGTTCACCTA
ATATGACCGACAATATCAAGGTAGTAGTAGCTAAGGTGGGAGCAATCATGCCAGATGGAATGATTATTTGGCCAGGCGAA
CTTCGTGGCGTTGAAAGTAATGGAATGATTTCCTCTGGAAGAGAATTGCATTTAAATAATGCACCTCAGAAGAAGGGAGC
ATTAATTTTACCTGATGATTTCGTTGTAGGTGAAGCATTTGATTTTGAAAAAGGTAATCATATCTTTGATTAA

Upstream 100 bases:

>100_bases
AGATGATTTAGATGACATGAAACCACTTCCTTTTCTACTAATTCTAACATTAGTAAGAAGGTTTTGAGAATTAAAAAGTA
TATATTTTAAGGAGAAAATA

Downstream 100 bases:

>100_bases
AGAAGGGATTTAGATGGTTAAATATGATGGGCCTGCTTTTTTTCGAAAAAAAGGCGTAGATTTTAATAATCCAAGTTTAA
ATATTCAAGAAGACCAAACT

Product: EMAP domain-containing protein

Products: AMP; diphosphate; L-phenylalanyl-tRNAPhe [C]

Alternate protein names: Partial phenylalanyl-tRNA synthetase; Partial PheRS; pFRS [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MLIASYNKETMGDILITMIRPDAEEAKIEQKGSVVRIADAKTNETTGFNFLNVSEIFEINGKGPITLTQEQVKQLNATLS
ANGFEAELVADERPKFVVGYVKSTKPHPDSDHLLITETEVDNGETLQIVSGSPNMTDNIKVVVAKVGAIMPDGMIIWPGE
LRGVESNGMISSGRELHLNNAPQKKGALILPDDFVVGEAFDFEKGNHIFD

Sequences:

>Translated_210_residues
MLIASYNKETMGDILITMIRPDAEEAKIEQKGSVVRIADAKTNETTGFNFLNVSEIFEINGKGPITLTQEQVKQLNATLS
ANGFEAELVADERPKFVVGYVKSTKPHPDSDHLLITETEVDNGETLQIVSGSPNMTDNIKVVVAKVGAIMPDGMIIWPGE
LRGVESNGMISSGRELHLNNAPQKKGALILPDDFVVGEAFDFEKGNHIFD
>Mature_210_residues
MLIASYNKETMGDILITMIRPDAEEAKIEQKGSVVRIADAKTNETTGFNFLNVSEIFEINGKGPITLTQEQVKQLNATLS
ANGFEAELVADERPKFVVGYVKSTKPHPDSDHLLITETEVDNGETLQIVSGSPNMTDNIKVVVAKVGAIMPDGMIIWPGE
LRGVESNGMISSGRELHLNNAPQKKGALILPDDFVVGEAFDFEKGNHIFD

Specific function: Unknown

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=91, Percent_Identity=36.2637362637363, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR002547 [H]

Pfam domain/function: PF01588 tRNA_bind [H]

EC number: 6.1.1.20 [C]

Molecular weight: Translated: 22898; Mature: 22898

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIASYNKETMGDILITMIRPDAEEAKIEQKGSVVRIADAKTNETTGFNFLNVSEIFEIN
CEEECCCCCCCCEEEEEEECCCHHHHHHHCCCCEEEEECCCCCCCCCCEEEEHHHEEEEC
GKGPITLTQEQVKQLNATLSANGFEAELVADERPKFVVGYVKSTKPHPDSDHLLITETEV
CCCCEEECHHHHHHHHCEECCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCEEEEEEEC
DNGETLQIVSGSPNMTDNIKVVVAKVGAIMPDGMIIWPGELRGVESNGMISSGRELHLNN
CCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEECC
APQKKGALILPDDFVVGEAFDFEKGNHIFD
CCCCCCEEECCCCEEECCEECCCCCCCCCC
>Mature Secondary Structure
MLIASYNKETMGDILITMIRPDAEEAKIEQKGSVVRIADAKTNETTGFNFLNVSEIFEIN
CEEECCCCCCCCEEEEEEECCCHHHHHHHCCCCEEEEECCCCCCCCCCEEEEHHHEEEEC
GKGPITLTQEQVKQLNATLSANGFEAELVADERPKFVVGYVKSTKPHPDSDHLLITETEV
CCCCEEECHHHHHHHHCEECCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCEEEEEEEC
DNGETLQIVSGSPNMTDNIKVVVAKVGAIMPDGMIIWPGELRGVESNGMISSGRELHLNN
CCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEECC
APQKKGALILPDDFVVGEAFDFEKGNHIFD
CCCCCCEEECCCCEEECCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.125 {2'-deoxyadenosine} 0.009 {ATP}} 0.2 {2-chloroadenosine} 0.11 {2-chloroadenosine} [C]

Substrates: ATP; @PHEN01.txt*phenylalanine!; tRNAPhe [C]

Specific reaction: ATP + @PHEN01.txt*phenylalanine! + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 3'-Deoxyadenosine 5'-triphosphate; 3'-Methyl adenosine 5'-triphosphate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]