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The map label for this gene is rfbX [H]

Identifier: 116492370

GI number: 116492370

Start: 630302

End: 631726

Strand: Direct

Name: rfbX [H]

Synonym: PEPE_0575

Alternate gene names: 116492370

Gene position: 630302-631726 (Clockwise)

Preceding gene: 116492369

Following gene: 116492371

Centisome position: 34.4

GC content: 29.47

Gene sequence:

>1425_bases
ATGAAAATAGTAAAAAACTTTCTTTTTAATATGACTTATCAAGTGTTTATTCTATTAGTTCCTTTGATAACAACGCCTTA
TATCTCTAGAGTGTTAGGCAGTGAGGGTGTAGGAATTAATGCTTATACTAATTCAATTATTCAATATTTTATATTATTTG
GAAGTATTGGAATTAGTACTTATGGTAATAGGCAAATAGCGTATTGTAGAGATGATGAAAGGGAATTAAGTAAAACTTTT
TGGGAGATATCTATTTTAAGGTTTGTTACAATTTTCATTGCATATATGGCGTTTATAATATATTTAAACTTAACAACCCA
ATACCATTCATATTTCATTGCTCAATCTTATCAAATTATAGCAGCAGCTTTTGATATATCTTGGTTTTTTATGGGTCTTG
AAAATTTCAAAATAACTGTTATTAGAAACTTTGTAGTAAAGATAATATCTCTAATCTGCATTTTTACGATGGTTAAAGAC
ACAAATGATATCGGCATCTATATATTAATACTTAGTCTCTCTCTATTATTCGGAAATCTATCTTTATGGGGCTATTTAAA
GAAATATATCAAAAAACCTGATTTTAAACAGCTTAAATTACTCAAGCATATTAAACCATCAGTGGCACTTTTTATCCCAC
AGATAGCGATCCAAATTTATTTGATTTTGAATAAAACTATGCTGGGTAGCATCAGTGGAGTACGAAGCGCAGGTTATTTT
GAATATTCAGATCGTATTGTGAAGATTGTCCTTGCTATAGTAACTTCAATCGGTACAGTTATGTTACCAAGGATGGCAAG
TATTTTTGCAAATAAAGATTATAAAAAATTACATGAATACATGTATACATCTGGTGATTTTATTAACTTTATTTCAATCC
CATTGTCATTTGGATTAGCGGCAGTTGCTCCTAAATTCTCTGTATGGTTTATGGGGAGTGAATTCTCGATAACTGGTAAA
TTAATGACAGTTGAAGCTATTGTTATTTTTTTGATTGGTTGGGGCGTTATGATTGGAAATCAATACTTAATTCCTACTAA
TCAAATTAGAAAATATACATATGCTGTAAGTGCGAGTGCAGTAGTTAATTTGGTGTTAAATGTCCCACTCATTTATAGTA
TGGGTGTTATGGGGGCAACAATTGCTACTGTTGCTTCGGAAATCACTTCTACAAGTTTGCAATTGTATTTTATTAGAAGA
CAAATAAATATACGCAAATTATTTCAAGGATTTTGGAAATACTTACTGGCAGGTTTAGTTATGTTTTTAGTAGTAAGGTT
TTTGAATGATCTATTAAGAATGACATTTTTATTATTGTTAGTAGAAGTTTTAGCCGGAGTAATAGTGTACGCTGGACTAT
GTTGGATTTTAAAAGTACCCTTTATTAAGGTTATAACTAATTTTATAAGTAAAAATAAACATTAA

Upstream 100 bases:

>100_bases
TATCGTTACTACAATATGGATCAAACGATTATGGCAGCTTTACAAACTGTTGAACATGAATTTGATAAATAAGCACTTGG
AAAAGGAACTGTGAAATACA

Downstream 100 bases:

>100_bases
ATTGTTTAAAAAGGGTGGGTTAATGGCTAAAAAAGTTTCTATTGTTATCCCCATCTACAATGTGGATGGTTTTTTAGAAA
GATGTCTAACTTCTGTAAAG

Product: PST family polysaccharide transporter

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MKIVKNFLFNMTYQVFILLVPLITTPYISRVLGSEGVGINAYTNSIIQYFILFGSIGISTYGNRQIAYCRDDERELSKTF
WEISILRFVTIFIAYMAFIIYLNLTTQYHSYFIAQSYQIIAAAFDISWFFMGLENFKITVIRNFVVKIISLICIFTMVKD
TNDIGIYILILSLSLLFGNLSLWGYLKKYIKKPDFKQLKLLKHIKPSVALFIPQIAIQIYLILNKTMLGSISGVRSAGYF
EYSDRIVKIVLAIVTSIGTVMLPRMASIFANKDYKKLHEYMYTSGDFINFISIPLSFGLAAVAPKFSVWFMGSEFSITGK
LMTVEAIVIFLIGWGVMIGNQYLIPTNQIRKYTYAVSASAVVNLVLNVPLIYSMGVMGATIATVASEITSTSLQLYFIRR
QINIRKLFQGFWKYLLAGLVMFLVVRFLNDLLRMTFLLLLVEVLAGVIVYAGLCWILKVPFIKVITNFISKNKH

Sequences:

>Translated_474_residues
MKIVKNFLFNMTYQVFILLVPLITTPYISRVLGSEGVGINAYTNSIIQYFILFGSIGISTYGNRQIAYCRDDERELSKTF
WEISILRFVTIFIAYMAFIIYLNLTTQYHSYFIAQSYQIIAAAFDISWFFMGLENFKITVIRNFVVKIISLICIFTMVKD
TNDIGIYILILSLSLLFGNLSLWGYLKKYIKKPDFKQLKLLKHIKPSVALFIPQIAIQIYLILNKTMLGSISGVRSAGYF
EYSDRIVKIVLAIVTSIGTVMLPRMASIFANKDYKKLHEYMYTSGDFINFISIPLSFGLAAVAPKFSVWFMGSEFSITGK
LMTVEAIVIFLIGWGVMIGNQYLIPTNQIRKYTYAVSASAVVNLVLNVPLIYSMGVMGATIATVASEITSTSLQLYFIRR
QINIRKLFQGFWKYLLAGLVMFLVVRFLNDLLRMTFLLLLVEVLAGVIVYAGLCWILKVPFIKVITNFISKNKH
>Mature_474_residues
MKIVKNFLFNMTYQVFILLVPLITTPYISRVLGSEGVGINAYTNSIIQYFILFGSIGISTYGNRQIAYCRDDERELSKTF
WEISILRFVTIFIAYMAFIIYLNLTTQYHSYFIAQSYQIIAAAFDISWFFMGLENFKITVIRNFVVKIISLICIFTMVKD
TNDIGIYILILSLSLLFGNLSLWGYLKKYIKKPDFKQLKLLKHIKPSVALFIPQIAIQIYLILNKTMLGSISGVRSAGYF
EYSDRIVKIVLAIVTSIGTVMLPRMASIFANKDYKKLHEYMYTSGDFINFISIPLSFGLAAVAPKFSVWFMGSEFSITGK
LMTVEAIVIFLIGWGVMIGNQYLIPTNQIRKYTYAVSASAVVNLVLNVPLIYSMGVMGATIATVASEITSTSLQLYFIRR
QINIRKLFQGFWKYLLAGLVMFLVVRFLNDLLRMTFLLLLVEVLAGVIVYAGLCWILKVPFIKVITNFISKNKH

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=395, Percent_Identity=24.0506329113924, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 54041; Mature: 54041

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7692219 [H]