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The map label for this gene is flp [H]

Identifier: 116492211

GI number: 116492211

Start: 455664

End: 456347

Strand: Reverse

Name: flp [H]

Synonym: PEPE_0408

Alternate gene names: 116492211

Gene position: 456347-455664 (Counterclockwise)

Preceding gene: 116492216

Following gene: 116492206

Centisome position: 24.9

GC content: 41.08

Gene sequence:

>684_bases
TTGCCTAAACAACTTCATCAGCATCACTGTGTTCCACTAGTCCCGATTTTTAAAAACTTAAATATCGAACAAACTGCTGT
AGTGGAATCCATCGTGCATCATCAACAACTTCCTGCCAATTCGGTCTTATATCAAGAAGGAAATCGAATTGACGCACTCT
CAATCATTGCCAACGGCCAAATTAAGGTCTTTCGTACTGCCGAAAATGGTAAAGAACAACTCTTATATCTCCTCCAAGCT
GGTGATATCAACGGTGAAGCCGCGCTCTTTAATCAAACCGAACATAACTCTACGGCTATTACGCTCACTCCCACAACGGT
TTGTACTATTAATCGCGCTGATTTTCAAAATTTACTTACGCGCTATCCCCAAATTGGAATGAACGTCATGAACACATTAG
GTAGCCGACTTTTAGAATTAGAGCGTCAAACGACTAAATCCAATACGGAATCCGTAGCAAGTCGTCTGGCCGACTACTTA
GTCGAGACCGCATCTGCTTTAAATCAAAATCCGGTTCGTTTACCCCTTAAAAACAAAGACTTAGCGACCCTCCTTGGCAC
TACACCTGAAACCATTAGTCGTTACTTCAAGCAATGGCACGAGCAGCATTTAATTACTAAACTAGAACGCCATCAAATCA
AGCTTAATGATGTGGATAATTTACTTTTAGCTACCAGTAACTAA

Upstream 100 bases:

>100_bases
AAGCATATAAGGTTTCAAACTAGTTGAAATTGACGTAGGTCAATTTTTTTAAATTTAATTTTAGCTATTATAAAAGTAAC
CTAACTCAGGAGGTCATATC

Downstream 100 bases:

>100_bases
ATAACCTATAATAAAAAAGATTGCCTAGTGAGCTATTCCTCACTAGGCAATCTTTTTAAACTCGTACTAAAATGTTATCC
ATTACATGTCCCGTAGTCTT

Product: Crp-like transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MPKQLHQHHCVPLVPIFKNLNIEQTAVVESIVHHQQLPANSVLYQEGNRIDALSIIANGQIKVFRTAENGKEQLLYLLQA
GDINGEAALFNQTEHNSTAITLTPTTVCTINRADFQNLLTRYPQIGMNVMNTLGSRLLELERQTTKSNTESVASRLADYL
VETASALNQNPVRLPLKNKDLATLLGTTPETISRYFKQWHEQHLITKLERHQIKLNDVDNLLLATSN

Sequences:

>Translated_227_residues
MPKQLHQHHCVPLVPIFKNLNIEQTAVVESIVHHQQLPANSVLYQEGNRIDALSIIANGQIKVFRTAENGKEQLLYLLQA
GDINGEAALFNQTEHNSTAITLTPTTVCTINRADFQNLLTRYPQIGMNVMNTLGSRLLELERQTTKSNTESVASRLADYL
VETASALNQNPVRLPLKNKDLATLLGTTPETISRYFKQWHEQHLITKLERHQIKLNDVDNLLLATSN
>Mature_226_residues
PKQLHQHHCVPLVPIFKNLNIEQTAVVESIVHHQQLPANSVLYQEGNRIDALSIIANGQIKVFRTAENGKEQLLYLLQAG
DINGEAALFNQTEHNSTAITLTPTTVCTINRADFQNLLTRYPQIGMNVMNTLGSRLLELERQTTKSNTESVASRLADYLV
ETASALNQNPVRLPLKNKDLATLLGTTPETISRYFKQWHEQHLITKLERHQIKLNDVDNLLLATSN

Specific function: This Protein Complexes With Cyclic AMP And Binds To Specific DNA Sites Near The Promoter To Regulate The Transcription Of Several Catabolite-Sensitive Operons. The Protein Induces A Severe Bend In The DNA. Acts As A Negative Regulator Of Its Own Synthesi

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=179, Percent_Identity=25.1396648044693, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25551; Mature: 25419

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQLHQHHCVPLVPIFKNLNIEQTAVVESIVHHQQLPANSVLYQEGNRIDALSIIANGQ
CCCCHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEEECCE
IKVFRTAENGKEQLLYLLQAGDINGEAALFNQTEHNSTAITLTPTTVCTINRADFQNLLT
EEEEEECCCCHHHEEEEEECCCCCCCEEEEECCCCCCEEEEECCCEEEEECHHHHHHHHH
RYPQIGMNVMNTLGSRLLELERQTTKSNTESVASRLADYLVETASALNQNPVRLPLKNKD
HCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LATLLGTTPETISRYFKQWHEQHLITKLERHQIKLNDVDNLLLATSN
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECC
>Mature Secondary Structure 
PKQLHQHHCVPLVPIFKNLNIEQTAVVESIVHHQQLPANSVLYQEGNRIDALSIIANGQ
CCCHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEEECCE
IKVFRTAENGKEQLLYLLQAGDINGEAALFNQTEHNSTAITLTPTTVCTINRADFQNLLT
EEEEEECCCCHHHEEEEEECCCCCCCEEEEECCCCCCEEEEECCCEEEEECHHHHHHHHH
RYPQIGMNVMNTLGSRLLELERQTTKSNTESVASRLADYLVETASALNQNPVRLPLKNKD
HCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LATLLGTTPETISRYFKQWHEQHLITKLERHQIKLNDVDNLLLATSN
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113923; 8441692 [H]