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The map label for this gene is xasA [C]

Identifier: 116492001

GI number: 116492001

Start: 224962

End: 226347

Strand: Direct

Name: xasA [C]

Synonym: PEPE_0190

Alternate gene names: 116492001

Gene position: 224962-226347 (Clockwise)

Preceding gene: 116492000

Following gene: 116492002

Centisome position: 12.28

GC content: 34.34

Gene sequence:

>1386_bases
ATGGAAAACGGGAAAAAGAAGTTTAGATTATTTGATGCAGTTTTAATGTCTGTAGTTGTTATTTTAGTCGTTGAGTCAGC
AGCTCCGGCCGCAGCGATTGGAACTTCGCAATTCTTTTGGTGGATCTTCCTACTTGTCATGTTTTTCCTACCATATGGAT
TAATTTCCTCAGAGCTAGGAACAACATATGCTGGAGACGGTGGCTTGTATGATTGGGTAAAAAAAGCTTTTGGTCCTAGA
TGGGGTGGAAGACTAGCTTGGTTATATTGGATTAATTATCCAATTTGGATGGCAAGCTTAGCGGTGTTATTTGCTCAAGT
AGCTGGTACAATTTTAAAATTAAAATTTAATACATTAACATCAATCATTATCCAATTAGTATTTGTTTGGTTTGTAGTGA
TTGTTGGAAATAAGCCTGCTTCTGAAAGTAAATGGATTATGAATCTGGCAGCATTCGCTAAAATTTTTACAATTTTATCT
TTAGCCATTTTGGGTATCTATGTAGCAATGACTAGAGGTGTTGCTAATGATCTTTCACCACACAACTTTTTACCACAAAT
GAATATTAGTAGCTTAAGTAATTTGTCGATTATTATTTTTAACTTCTTAGGATTTGAAGTGGTTGCGACGATGGCTGATG
ATATGGATGACCCTAAAAAGCAAATACCAAAGGCCATTATTTACGGCGGCATTTTAATTGCATTCTTCTACCTGCTTTCT
GCATTTGGTATGAGTGCTGCAGTGCCAACTGATAAGCTTTCAGCTTCAAGTGGGTTGTTAGATAGTTTCATATTAATGAT
TGGTAATATGAATTGGTTTGTAGTGATTATCGGAATTTTATTCTTGTACATCTTGGTTTCAGAAATGGTTTCTTGGGCGT
TAGGAGTTAATTATGTAGCAGACTACGCTGCTAAAGACCATGTTTTACCAAATATCTTTGGAAAAGAAGATAAAAATAAC
ATGCCGATTGGTACAGGATATATAAATGGTATTGTTGCTACTATTTTAGTAGTATCTGCTCCATTTATTCCTAATCAAGA
TATTTTCTGGGCATTTTTCTCTTTGAACGTAGTTGCTCTATTATTGTCATATACAATGATGTTCCCAGCTTTTCTTAAAT
TAAGAAAAACTGACCCTAATCAAGAACGACCTTTTAAAGTGCCTGGTGGAAAAGTAATGATACAGTTAATGACGTGGGTT
CCAGAGATATTGCTATTTATTACAATAATTTTTACAATTGTTCCACTTAATACAGGTAAATCAGAAATGGGAACTAAAAT
ACCAATTCTGATTGGTGTAGTTATTACATTAGCAGTTGGCGAAATTTTTGTACGAGTTGCTGAACATCGTGAGAAGAGTT
TAAAACAAAAGGAGAATATAAAATGA

Upstream 100 bases:

>100_bases
ATGATGCAGAATTTGAATTGATGTTAAAAAATGCAGTAGATTCACGTAATAACTAGACCCGTTACATCGTTTTAAACGAT
GCAGATTGGAGATAAAGATT

Downstream 100 bases:

>100_bases
AAACATTAGACAGTACCCCTAAAAAAGATGGATATAGGATGCCGGGTGAATTTGAAGAACATTCAGGAGCGTATATGCTT
TGGCCACAGAGACCAGATAA

Product: amino acid transporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MENGKKKFRLFDAVLMSVVVILVVESAAPAAAIGTSQFFWWIFLLVMFFLPYGLISSELGTTYAGDGGLYDWVKKAFGPR
WGGRLAWLYWINYPIWMASLAVLFAQVAGTILKLKFNTLTSIIIQLVFVWFVVIVGNKPASESKWIMNLAAFAKIFTILS
LAILGIYVAMTRGVANDLSPHNFLPQMNISSLSNLSIIIFNFLGFEVVATMADDMDDPKKQIPKAIIYGGILIAFFYLLS
AFGMSAAVPTDKLSASSGLLDSFILMIGNMNWFVVIIGILFLYILVSEMVSWALGVNYVADYAAKDHVLPNIFGKEDKNN
MPIGTGYINGIVATILVVSAPFIPNQDIFWAFFSLNVVALLLSYTMMFPAFLKLRKTDPNQERPFKVPGGKVMIQLMTWV
PEILLFITIIFTIVPLNTGKSEMGTKIPILIGVVITLAVGEIFVRVAEHREKSLKQKENIK

Sequences:

>Translated_461_residues
MENGKKKFRLFDAVLMSVVVILVVESAAPAAAIGTSQFFWWIFLLVMFFLPYGLISSELGTTYAGDGGLYDWVKKAFGPR
WGGRLAWLYWINYPIWMASLAVLFAQVAGTILKLKFNTLTSIIIQLVFVWFVVIVGNKPASESKWIMNLAAFAKIFTILS
LAILGIYVAMTRGVANDLSPHNFLPQMNISSLSNLSIIIFNFLGFEVVATMADDMDDPKKQIPKAIIYGGILIAFFYLLS
AFGMSAAVPTDKLSASSGLLDSFILMIGNMNWFVVIIGILFLYILVSEMVSWALGVNYVADYAAKDHVLPNIFGKEDKNN
MPIGTGYINGIVATILVVSAPFIPNQDIFWAFFSLNVVALLLSYTMMFPAFLKLRKTDPNQERPFKVPGGKVMIQLMTWV
PEILLFITIIFTIVPLNTGKSEMGTKIPILIGVVITLAVGEIFVRVAEHREKSLKQKENIK
>Mature_461_residues
MENGKKKFRLFDAVLMSVVVILVVESAAPAAAIGTSQFFWWIFLLVMFFLPYGLISSELGTTYAGDGGLYDWVKKAFGPR
WGGRLAWLYWINYPIWMASLAVLFAQVAGTILKLKFNTLTSIIIQLVFVWFVVIVGNKPASESKWIMNLAAFAKIFTILS
LAILGIYVAMTRGVANDLSPHNFLPQMNISSLSNLSIIIFNFLGFEVVATMADDMDDPKKQIPKAIIYGGILIAFFYLLS
AFGMSAAVPTDKLSASSGLLDSFILMIGNMNWFVVIIGILFLYILVSEMVSWALGVNYVADYAAKDHVLPNIFGKEDKNN
MPIGTGYINGIVATILVVSAPFIPNQDIFWAFFSLNVVALLLSYTMMFPAFLKLRKTDPNQERPFKVPGGKVMIQLMTWV
PEILLFITIIFTIVPLNTGKSEMGTKIPILIGVVITLAVGEIFVRVAEHREKSLKQKENIK

Specific function: Probable Amino Acid Transporter. Involved In Extreme Acid Resistance. [C]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=408, Percent_Identity=25, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI87081798, Length=271, Percent_Identity=23.2472324723247, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI2367353, Length=332, Percent_Identity=25, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87082380, Length=263, Percent_Identity=25.0950570342205, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 51228; Mature: 51228

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8366052 [H]