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The map label for this gene is ptcA

Identifier: 116492000

GI number: 116492000

Start: 223883

End: 224917

Strand: Direct

Name: ptcA

Synonym: PEPE_0189

Alternate gene names: 116492000

Gene position: 223883-224917 (Clockwise)

Preceding gene: 116491999

Following gene: 116492001

Centisome position: 12.22

GC content: 35.65

Gene sequence:

>1035_bases
ATGAATACAAAAAGAGATTTTATTGACACTAATACATATACACAAGAGGAAATCACATACATGATTAATCTTGGTCTTAA
GATAAAAGAATCAATTAAGAATGGTTACTATCCACCACTTCTAAAAAATAAGACACTTGGCATGATTTTTGAACAAAGCT
CAACTAGAACTAGAACTTCTGCTGAAGCTGCAATGACAGAATTAGGTGGACATGCCCAATACTTAGCACCAGGACAAATT
CAATTAGGTGGGCATGAAACAATTGAAGATACGTCACAAGTTCTCGGACGGATTTTAGATATCATCGGAGCACGTGTTGA
TCGTCATAAGACAGTAGCTGAAGTTGGTAAATATGCTAAGGTTCCGGTAGTTAACTTTATGAGTGATTATAATCATCCAA
CTCAAGAATTAGGTGATATTACAACAATGGTTGAACATTTACCAGCAGGTAAAAAACTAAGCGATTGTAAAATTGTTTTT
GTCGGTGATGCAACCCAAGTGTGTGTTTCAACGATGTTTATGACAACTAAAATGGGAATGGACTTTGTTCAATTCGGACC
AAAAGGGTTCCAAATGAAAGATGATGTAGTTGAAATTGGGAAAGAAAATGCGAAGAAATATGGTGGTAGCGTAACAATTA
CTGAAGATGCTGATGAAGCAATGAAAGATGCTGATTTTGTCTATACAGATGTATGGTATGGTCTCTACGATGACGAAATG
CCAAAAGAAGAACGCATGAACATTTTCTATCCTAAATATCAAGTTAATGCTGAATTAATGGCTAAAGCTTCAGATCATGT
TAAATTTATGCACTGTCTTCCAGCTACACGTGGCGAAGAAGTAACGGATGAAGTACTAGACTCAGATTACTCGATTGTTT
GGGATGAAGCAGAAAATAGAAAGACTGCAATGCGGGCAATCTTCGTATATTTACTTAACCCATCACTAAATTATGCTTCA
AAAGCGGTCGCAGAAAAATATGATGCAGAATTTGAATTGATGTTAAAAAATGCAGTAGATTCACGTAATAACTAG

Upstream 100 bases:

>100_bases
ACCCCGTTGCAAGGCCTTTGGAAACGCTTTATTTATATTTGCAATCAATTTATGATAAGCATGTAAACAAAATAAAGCAA
AACGAAAGGGAGTACCAGTT

Downstream 100 bases:

>100_bases
ACCCGTTACATCGTTTTAAACGATGCAGATTGGAGATAAAGATTATGGAAAACGGGAAAAAGAAGTTTAGATTATTTGAT
GCAGTTTTAATGTCTGTAGT

Product: putrescine carbamoyltransferase

Products: NA

Alternate protein names: PTC; PTCase; Putrescine transcarbamoylase; Putrescine transcarbamylase

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MNTKRDFIDTNTYTQEEITYMINLGLKIKESIKNGYYPPLLKNKTLGMIFEQSSTRTRTSAEAAMTELGGHAQYLAPGQI
QLGGHETIEDTSQVLGRILDIIGARVDRHKTVAEVGKYAKVPVVNFMSDYNHPTQELGDITTMVEHLPAGKKLSDCKIVF
VGDATQVCVSTMFMTTKMGMDFVQFGPKGFQMKDDVVEIGKENAKKYGGSVTITEDADEAMKDADFVYTDVWYGLYDDEM
PKEERMNIFYPKYQVNAELMAKASDHVKFMHCLPATRGEEVTDEVLDSDYSIVWDEAENRKTAMRAIFVYLLNPSLNYAS
KAVAEKYDAEFELMLKNAVDSRNN

Sequences:

>Translated_344_residues
MNTKRDFIDTNTYTQEEITYMINLGLKIKESIKNGYYPPLLKNKTLGMIFEQSSTRTRTSAEAAMTELGGHAQYLAPGQI
QLGGHETIEDTSQVLGRILDIIGARVDRHKTVAEVGKYAKVPVVNFMSDYNHPTQELGDITTMVEHLPAGKKLSDCKIVF
VGDATQVCVSTMFMTTKMGMDFVQFGPKGFQMKDDVVEIGKENAKKYGGSVTITEDADEAMKDADFVYTDVWYGLYDDEM
PKEERMNIFYPKYQVNAELMAKASDHVKFMHCLPATRGEEVTDEVLDSDYSIVWDEAENRKTAMRAIFVYLLNPSLNYAS
KAVAEKYDAEFELMLKNAVDSRNN
>Mature_344_residues
MNTKRDFIDTNTYTQEEITYMINLGLKIKESIKNGYYPPLLKNKTLGMIFEQSSTRTRTSAEAAMTELGGHAQYLAPGQI
QLGGHETIEDTSQVLGRILDIIGARVDRHKTVAEVGKYAKVPVVNFMSDYNHPTQELGDITTMVEHLPAGKKLSDCKIVF
VGDATQVCVSTMFMTTKMGMDFVQFGPKGFQMKDDVVEIGKENAKKYGGSVTITEDADEAMKDADFVYTDVWYGLYDDEM
PKEERMNIFYPKYQVNAELMAKASDHVKFMHCLPATRGEEVTDEVLDSDYSIVWDEAENRKTAMRAIFVYLLNPSLNYAS
KAVAEKYDAEFELMLKNAVDSRNN

Specific function: Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family. PTCase subfamily

Homologues:

Organism=Homo sapiens, GI38788445, Length=309, Percent_Identity=37.8640776699029, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI18105007, Length=298, Percent_Identity=21.8120805369128, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786469, Length=330, Percent_Identity=36.969696969697, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1790703, Length=329, Percent_Identity=35.5623100303951, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI48994908, Length=318, Percent_Identity=28.6163522012579, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI2367364, Length=327, Percent_Identity=25.0764525993884, Blast_Score=71, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6322373, Length=322, Percent_Identity=31.9875776397516, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24642586, Length=290, Percent_Identity=24.4827586206897, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTC_PEDPA (Q03HM9)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_803735.1
- ProteinModelPortal:   Q03HM9
- SMR:   Q03HM9
- STRING:   Q03HM9
- GeneID:   4418633
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0189
- NMPDR:   fig|278197.10.peg.173
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   GGHETIE
- PhylomeDB:   Q03HM9
- ProtClustDB:   PRK02255
- BioCyc:   PPEN278197:PEPE_0189-MONOMER
- HAMAP:   MF_02102
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.6

Molecular weight: Translated: 38812; Mature: 38812

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 105-105 BINDING 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKRDFIDTNTYTQEEITYMINLGLKIKESIKNGYYPPLLKNKTLGMIFEQSSTRTRTS
CCCCCCCCCCCCCCHHHEEEEEECCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHH
AEAAMTELGGHAQYLAPGQIQLGGHETIEDTSQVLGRILDIIGARVDRHKTVAEVGKYAK
HHHHHHHHCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCC
VPVVNFMSDYNHPTQELGDITTMVEHLPAGKKLSDCKIVFVGDATQVCVSTMFMTTKMGM
CCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCC
DFVQFGPKGFQMKDDVVEIGKENAKKYGGSVTITEDADEAMKDADFVYTDVWYGLYDDEM
HHHHCCCCCCCCCHHHHHHCHHHHHHCCCEEEEECCHHHHHHHCCEEEEHHHCCCCCCCC
PKEERMNIFYPKYQVNAELMAKASDHVKFMHCLPATRGEEVTDEVLDSDYSIVWDEAENR
CHHHCCEEECCCEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEECCCCCH
KTAMRAIFVYLLNPSLNYASKAVAEKYDAEFELMLKNAVDSRNN
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNTKRDFIDTNTYTQEEITYMINLGLKIKESIKNGYYPPLLKNKTLGMIFEQSSTRTRTS
CCCCCCCCCCCCCCHHHEEEEEECCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHH
AEAAMTELGGHAQYLAPGQIQLGGHETIEDTSQVLGRILDIIGARVDRHKTVAEVGKYAK
HHHHHHHHCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCC
VPVVNFMSDYNHPTQELGDITTMVEHLPAGKKLSDCKIVFVGDATQVCVSTMFMTTKMGM
CCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCC
DFVQFGPKGFQMKDDVVEIGKENAKKYGGSVTITEDADEAMKDADFVYTDVWYGLYDDEM
HHHHCCCCCCCCCHHHHHHCHHHHHHCCCEEEEECCHHHHHHHCCEEEEHHHCCCCCCCC
PKEERMNIFYPKYQVNAELMAKASDHVKFMHCLPATRGEEVTDEVLDSDYSIVWDEAENR
CHHHCCEEECCCEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEECCCCCH
KTAMRAIFVYLLNPSLNYASKAVAEKYDAEFELMLKNAVDSRNN
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA