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The map label for this gene is rihC [H]

Identifier: 116491975

GI number: 116491975

Start: 190675

End: 191610

Strand: Reverse

Name: rihC [H]

Synonym: PEPE_0161

Alternate gene names: 116491975

Gene position: 191610-190675 (Counterclockwise)

Preceding gene: 116491993

Following gene: 116491974

Centisome position: 10.46

GC content: 35.26

Gene sequence:

>936_bases
ATGTTGATGAATAATTTAAAAGCACAACCTGTTATTATCAGTACTGATCCTGGTATCGATGATGCAGTTGCAATATCTTT
GGCACTTTTTAGTAACGATATAGATGTCAAACTTATATGTCCTATTGCGGGTAATGTAAGTTTAGAAAAAACAACTCATA
ATACACAAAAGCTTCTAACTTTTTTTAACAAAGAAATACCTATTGTTCCAGGAAGCCCGTCACCTCTCCTTCGACAAAAA
CAAAATGCTAGTAACGTTCATGGTGAATCTGGCATGGATGGCTTTGATTTTCCTATCCCTAAAATCACACCTGATGTATC
AAGGACCGCGGTACAAGCAATGCATGAAACTGTATCAAACAGTACTGTTCCTGTAACTTTAATGGGACTTGGACCTTTAA
CTGATATTGCACTTTATCTTCATCTCTATCCACAGGATAGTAAGAATATCCACGAAATTGTATTAATGGGAGGAGCTTTA
GGTAGAGGTAACTTGGGAGTCTTATCTGAGTTTAACTTTGGAGTTGACCCTGAAGCAGCAAAAATAGTATTTAACAGTAA
TATTTCAATACGTATTGTACCCATGGAAATAGGGAGACAAGCTAAAATAATGCCCGCCACTTCAGAAAAAATAAAAAATC
TTGGAAAAACCGGTAAAATGTTTTATTCACTTTTTTCTCATTATCGTGGAGGTAGTTTTGATAAGGGATTAAATATGTAT
GACGTCCTCGCTTTAGGGTTGATTTTAAAACCAGAAATATTTGAGATGAAATCAACCTATGTTGCTATAGAAACCCAAGG
AGCATTTACAGCAGGTGCTTCATTAATTGATCTTAAGGGATACCTTGGGGAAAATTCCAATGCAGAGGTGGCAATTAACG
TTGATACTAAACAATTTGAAGACTGGTTCACTAAAATGATATCAAAAACAATATAG

Upstream 100 bases:

>100_bases
AACCCTCAAAATTAAATACAACGTTGTATTTTATAATACAACGTTGTATAATAATTTCAGAAAACGTTTTCAAAAATATA
ACTAAGGAGTTATTAAACCT

Downstream 100 bases:

>100_bases
GAGGCGATCTCATGCACGCAGTATTAATCTCTATCATTGGTATTATAGCTATAAGCATCTTGATTTATATGTTATTAAAG
AAAAATGATATTAAAATGAT

Product: inosine-uridine nucleoside N-ribohydrolase

Products: uracil; ribose; xanthine; hypoxanthine; adenine; cytosine; guanine [C]

Alternate protein names: Purine/pyrimidine ribonucleoside hydrolase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MLMNNLKAQPVIISTDPGIDDAVAISLALFSNDIDVKLICPIAGNVSLEKTTHNTQKLLTFFNKEIPIVPGSPSPLLRQK
QNASNVHGESGMDGFDFPIPKITPDVSRTAVQAMHETVSNSTVPVTLMGLGPLTDIALYLHLYPQDSKNIHEIVLMGGAL
GRGNLGVLSEFNFGVDPEAAKIVFNSNISIRIVPMEIGRQAKIMPATSEKIKNLGKTGKMFYSLFSHYRGGSFDKGLNMY
DVLALGLILKPEIFEMKSTYVAIETQGAFTAGASLIDLKGYLGENSNAEVAINVDTKQFEDWFTKMISKTI

Sequences:

>Translated_311_residues
MLMNNLKAQPVIISTDPGIDDAVAISLALFSNDIDVKLICPIAGNVSLEKTTHNTQKLLTFFNKEIPIVPGSPSPLLRQK
QNASNVHGESGMDGFDFPIPKITPDVSRTAVQAMHETVSNSTVPVTLMGLGPLTDIALYLHLYPQDSKNIHEIVLMGGAL
GRGNLGVLSEFNFGVDPEAAKIVFNSNISIRIVPMEIGRQAKIMPATSEKIKNLGKTGKMFYSLFSHYRGGSFDKGLNMY
DVLALGLILKPEIFEMKSTYVAIETQGAFTAGASLIDLKGYLGENSNAEVAINVDTKQFEDWFTKMISKTI
>Mature_311_residues
MLMNNLKAQPVIISTDPGIDDAVAISLALFSNDIDVKLICPIAGNVSLEKTTHNTQKLLTFFNKEIPIVPGSPSPLLRQK
QNASNVHGESGMDGFDFPIPKITPDVSRTAVQAMHETVSNSTVPVTLMGLGPLTDIALYLHLYPQDSKNIHEIVLMGGAL
GRGNLGVLSEFNFGVDPEAAKIVFNSNISIRIVPMEIGRQAKIMPATSEKIKNLGKTGKMFYSLFSHYRGGSFDKGLNMY
DVLALGLILKPEIFEMKSTYVAIETQGAFTAGASLIDLKGYLGENSNAEVAINVDTKQFEDWFTKMISKTI

Specific function: Hydrolyzes both purine and pyrimidine ribonucleosides with a broad-substrate specificity [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786213, Length=299, Percent_Identity=43.4782608695652, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1786871, Length=301, Percent_Identity=37.8737541528239, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788486, Length=311, Percent_Identity=31.1897106109325, Blast_Score=164, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI32565474, Length=261, Percent_Identity=29.5019157088123, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17565698, Length=299, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320608, Length=258, Percent_Identity=29.4573643410853, Blast_Score=106, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24641839, Length=296, Percent_Identity=25.3378378378378, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24583915, Length=326, Percent_Identity=26.6871165644172, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24641837, Length=187, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022976 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33823; Mature: 33823

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMNNLKAQPVIISTDPGIDDAVAISLALFSNDIDVKLICPIAGNVSLEKTTHNTQKLLT
CCCCCCCCCCEEEECCCCCCHHHEEEEEEECCCCCEEEEEECCCCEEEEECCCHHHHHHH
FFNKEIPIVPGSPSPLLRQKQNASNVHGESGMDGFDFPIPKITPDVSRTAVQAMHETVSN
HHCCCCCEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
STVPVTLMGLGPLTDIALYLHLYPQDSKNIHEIVLMGGALGRGNLGVLSEFNFGVDPEAA
CCCEEEEEECCCHHEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCE
KIVFNSNISIRIVPMEIGRQAKIMPATSEKIKNLGKTGKMFYSLFSHYRGGSFDKGLNMY
EEEEECCCEEEEEECCCCCCEEECCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHH
DVLALGLILKPEIFEMKSTYVAIETQGAFTAGASLIDLKGYLGENSNAEVAINVDTKQFE
HHHHHHHHCCCHHEEECCEEEEEEECCCEECCCEEEEEEHHCCCCCCCEEEEEECHHHHH
DWFTKMISKTI
HHHHHHHHHCC
>Mature Secondary Structure
MLMNNLKAQPVIISTDPGIDDAVAISLALFSNDIDVKLICPIAGNVSLEKTTHNTQKLLT
CCCCCCCCCCEEEECCCCCCHHHEEEEEEECCCCCEEEEEECCCCEEEEECCCHHHHHHH
FFNKEIPIVPGSPSPLLRQKQNASNVHGESGMDGFDFPIPKITPDVSRTAVQAMHETVSN
HHCCCCCEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
STVPVTLMGLGPLTDIALYLHLYPQDSKNIHEIVLMGGALGRGNLGVLSEFNFGVDPEAA
CCCEEEEEECCCHHEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCE
KIVFNSNISIRIVPMEIGRQAKIMPATSEKIKNLGKTGKMFYSLFSHYRGGSFDKGLNMY
EEEEECCCEEEEEECCCCCCEEECCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHH
DVLALGLILKPEIFEMKSTYVAIETQGAFTAGASLIDLKGYLGENSNAEVAINVDTKQFE
HHHHHHHHCCCHHEEECCEEEEEEECCCEECCCEEEEEEHHCCCCCCCEEEEEECHHHHH
DWFTKMISKTI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; xanthosine; inosine; adenosine; cytidine; guanosine [C]

Specific reaction: uridine + H2O = uracil + ribose xanthosine + H2O = xanthine + ribose inosine + H2O = hypoxanthine + ribose adenosine + H2O = adenine + ribose cytidine + H2O = cytosine + ribose guanosine + H2O = guanine + ribose [C]

General reaction: Nonspecific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA