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The map label for this gene is icaA [H]

Identifier: 116491940

GI number: 116491940

Start: 150668

End: 151687

Strand: Direct

Name: icaA [H]

Synonym: PEPE_0126

Alternate gene names: 116491940

Gene position: 150668-151687 (Clockwise)

Preceding gene: 116491939

Following gene: 116491941

Centisome position: 8.22

GC content: 41.76

Gene sequence:

>1020_bases
ATGGATGTCACGGCAATCGTACCTGCCCATAATGAAGGTGCAAATATTGAAGCAACGATTAAAGCGTTAAAGCAACAAGT
TCGAAAAGTGATTGTGGCGTGTGATCACTGTACAGATGATACTTATCAAAAAGCAATTCAGGCAGGAGCGGTGGCTTTTG
AAACGGTTGATAATCAGCATCGTAAAGCAGGAGCATTAAATCAAGCCTTAGAAAATTACGTTGATTGGCAATTACCCGAC
CAATATATTTTCATTTGTGATGCTGACACGGTGATTGCGGATGATTGGATTGAACGGGCCCAGATAGCGATTGATGCTGG
TGGTTATGATGCGGTCGGTTCAGTGTTTTTCGGGGATGCGGATCGAAGTTCGATGATTGAATTTTGTCAGCAATTGGAGT
GGTACCGCTACACCAATCAGATTAAGCGGAGCAAAAAAGTTTTTGTTTTAACCGGGACCGCTTCGATGGTAAGTGCCGAG
ATGTTTCAGAAAGTTAAAGCTCGCAAAGGTAGTTTTTACGACGAAGACTCGATTACCGAGGACTTTGCGTTGACGATTGA
TTTAAAAGAGGCGGGGGCCAGAATCATTTCCCCAATCTCTTGTTACTGCACGACGGAAATTATGCCCAACTGGCGACTAC
TATTTTTACAACGGCGACGCTGGTATTTAGGAGCACTCCAACAAATGGTTCGCCGAAAATGGGATCGCGTTTTATTTCCA
TACGTTTTTCAACAATTAATGTTACTTATTTCAGTCCTAGCATTTATGGGACTGATTGTTTTCACCGGTTACTTAATGGT
GCAAGACTTGATTATTTTTAGTTTCTTTTGGTTTTTAATTGGAGTGATTTTTGCAGTCGAGCGGGTGATTACGATTTGGA
ACCAAGACCGGCAGGCAAAGATATTTGCATTAATGATTTTTCCAGAATTAATTTATAGTTTATTTTTACAAATTGCGTAC
CTTGGGGCGCTGGTTCAGCTGTTAAGCGGGTCAGCGGGTACGTGGAATCATCTAGATTAA

Upstream 100 bases:

>100_bases
ACAACGACGATGAAGCTGACCAATAGTATTTATTCATTGGTCAATCAAACTCCATATTTAGGTGGAAACACGTATTAATT
AAGGAGAAGGTAAGTTAAAC

Downstream 100 bases:

>100_bases
TGATTTAATTCAATAGGGGGACGAAAAAGTTATGTATTCAAATATTCAAAGAAGTGGTGTAGCTTTTGTAAGCTTAAGTT
GGGGGTTCATGTATCACCTA

Product: glycosyltransferase

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MDVTAIVPAHNEGANIEATIKALKQQVRKVIVACDHCTDDTYQKAIQAGAVAFETVDNQHRKAGALNQALENYVDWQLPD
QYIFICDADTVIADDWIERAQIAIDAGGYDAVGSVFFGDADRSSMIEFCQQLEWYRYTNQIKRSKKVFVLTGTASMVSAE
MFQKVKARKGSFYDEDSITEDFALTIDLKEAGARIISPISCYCTTEIMPNWRLLFLQRRRWYLGALQQMVRRKWDRVLFP
YVFQQLMLLISVLAFMGLIVFTGYLMVQDLIIFSFFWFLIGVIFAVERVITIWNQDRQAKIFALMIFPELIYSLFLQIAY
LGALVQLLSGSAGTWNHLD

Sequences:

>Translated_339_residues
MDVTAIVPAHNEGANIEATIKALKQQVRKVIVACDHCTDDTYQKAIQAGAVAFETVDNQHRKAGALNQALENYVDWQLPD
QYIFICDADTVIADDWIERAQIAIDAGGYDAVGSVFFGDADRSSMIEFCQQLEWYRYTNQIKRSKKVFVLTGTASMVSAE
MFQKVKARKGSFYDEDSITEDFALTIDLKEAGARIISPISCYCTTEIMPNWRLLFLQRRRWYLGALQQMVRRKWDRVLFP
YVFQQLMLLISVLAFMGLIVFTGYLMVQDLIIFSFFWFLIGVIFAVERVITIWNQDRQAKIFALMIFPELIYSLFLQIAY
LGALVQLLSGSAGTWNHLD
>Mature_339_residues
MDVTAIVPAHNEGANIEATIKALKQQVRKVIVACDHCTDDTYQKAIQAGAVAFETVDNQHRKAGALNQALENYVDWQLPD
QYIFICDADTVIADDWIERAQIAIDAGGYDAVGSVFFGDADRSSMIEFCQQLEWYRYTNQIKRSKKVFVLTGTASMVSAE
MFQKVKARKGSFYDEDSITEDFALTIDLKEAGARIISPISCYCTTEIMPNWRLLFLQRRRWYLGALQQMVRRKWDRVLFP
YVFQQLMLLISVLAFMGLIVFTGYLMVQDLIIFSFFWFLIGVIFAVERVITIWNQDRQAKIFALMIFPELIYSLFLQIAY
LGALVQLLSGSAGTWNHLD

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 38798; Mature: 38798

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVTAIVPAHNEGANIEATIKALKQQVRKVIVACDHCTDDTYQKAIQAGAVAFETVDNQH
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHH
RKAGALNQALENYVDWQLPDQYIFICDADTVIADDWIERAQIAIDAGGYDAVGSVFFGDA
HHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHEEEECCCCHHHHHHEECCC
DRSSMIEFCQQLEWYRYTNQIKRSKKVFVLTGTASMVSAEMFQKVKARKGSFYDEDSITE
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCC
DFALTIDLKEAGARIISPISCYCTTEIMPNWRLLFLQRRRWYLGALQQMVRRKWDRVLFP
CEEEEEEHHHHCHHHHCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVFQQLMLLISVLAFMGLIVFTGYLMVQDLIIFSFFWFLIGVIFAVERVITIWNQDRQAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
IFALMIFPELIYSLFLQIAYLGALVQLLSGSAGTWNHLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDVTAIVPAHNEGANIEATIKALKQQVRKVIVACDHCTDDTYQKAIQAGAVAFETVDNQH
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHH
RKAGALNQALENYVDWQLPDQYIFICDADTVIADDWIERAQIAIDAGGYDAVGSVFFGDA
HHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHEEEECCCCHHHHHHEECCC
DRSSMIEFCQQLEWYRYTNQIKRSKKVFVLTGTASMVSAEMFQKVKARKGSFYDEDSITE
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCC
DFALTIDLKEAGARIISPISCYCTTEIMPNWRLLFLQRRRWYLGALQQMVRRKWDRVLFP
CEEEEEEHHHHCHHHHCCHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVFQQLMLLISVLAFMGLIVFTGYLMVQDLIIFSFFWFLIGVIFAVERVITIWNQDRQAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
IFALMIFPELIYSLFLQIAYLGALVQLLSGSAGTWNHLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]