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The map label for this gene is icaA [H]

Identifier: 116491922

GI number: 116491922

Start: 112632

End: 113957

Strand: Direct

Name: icaA [H]

Synonym: PEPE_0105

Alternate gene names: 116491922

Gene position: 112632-113957 (Clockwise)

Preceding gene: 116491921

Following gene: 116491923

Centisome position: 6.15

GC content: 40.42

Gene sequence:

>1326_bases
ATGTCATCAATCTTTATTGATAGTATTTTCAAAGGATCCCTGATTGATACGACCATTAACATTATTCTTTTGATACTTTG
TTTATATCCTGTAATCGGGGCGTTCTTCTGGTTTACAGGTTCATTGTCGTATCGCTTTTTGAAAACCCATAAACGGGATG
AAGGCTGGGAAAATATTCTACCTGCGGACCAACCATTAATTACCATCATGATTCCAGCCCATAATGAAGAAGTCATGATT
GAAGAAACGATCACCTATCTTTTTAACGAAATTAATTACACTAATTTTGAAGTTTTAGTCGCTGACGATGGCTCGACGGA
TCAAACGGGAGCAATCTTAGCACGCTTGAGAAAACAATATCCACGCCTGCGGACGATTAAAATCTTGAAAAATAAAGGGA
AGGCGCATGCTTTTAACATTGGAATGTATTTTGCAAAAGGGAAATACATCCTTAGTAATGATGCTGATACGATTCCCGAA
CCGGATGCTTTGATGAAGTACATGAACTTTTTCATGCATGACCGGGATATGAATACTTCGGCGGTAACAGCTAATATGGA
TGTTCAAAACCGCACGAAGATTTTAGGCAAGGCCCAGACGGTTGAATTTTCGAGTATTGTTGGGGTCATTAAACGCAGTC
AAACTTCAATTAACGACTCGATGTATGCCTACAGTGGCGCCAACACGTTGTATCGCAAGAGTTTTCTGATTGACGTGGGA
GGCTTCCGGCAAAATCGGGCGACCGAAGATATTAGTATTGCCTGGGATCACCAGATGATTGGGGCAATTCCGCGGTTTAC
TCCTAATATTGTCTTTCACATGAATGTCCCCGAATCTCTTAAAGATCTTTATCACCAACGTAAGCGTTGGGCGCAAGGGG
GAACTGAAGTGTGGTTGACCAATATTAAGAAGTTCCTCTTACATCCGATTAAGCATCGTTATCAAATGTCGATGTTTATT
GACTCAACCCTTTCCATTATCTGGTCATTTTTCTTTATGATTACCACCGCTGTTTTTATCATTACCATGTTGGTTTTCTT
ACTGACGGGGAATTACGAACGGGTCTTTCACGGAATCGTGATGTCGTTTATCTTTGTGACCTTTGAGCTACTGGCCGGGT
TCTTACAATTATTAGCGGCTTTAATTTTGGATCATCATGCTGCTAAGTTAAAATACCTTATTTTTGCGCCGCTTTATATG
CTCTTTTTCTGGATGGTCAATCCCGTGACGGTTGTCATGACGTTTGTACCGGCTCTTAAAACGATTTTTGGTTTTGGTTC
TGGGACTTGGATTAGTCCCGAACGTAAATCACTTCAAAATAAATAA

Upstream 100 bases:

>100_bases
CAGGTACTACGAAGTAAAACCAGAACAAAATCTGGATACAGACGAAATTAAAGAAATTTATCGAGCATCTAATTTAGACG
ATCTTGGATAAGGAGCAAAA

Downstream 100 bases:

>100_bases
AACATATTCCGTTTTATCTGCGGAATATGTTTTTTGTTTGCCGTGAAAGCTATACAAAAAATGGAAAATAGTTCAAACTT
ATCTATGAAAAGGCTTTCTA

Product: glycosyltransferase

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSSIFIDSIFKGSLIDTTINIILLILCLYPVIGAFFWFTGSLSYRFLKTHKRDEGWENILPADQPLITIMIPAHNEEVMI
EETITYLFNEINYTNFEVLVADDGSTDQTGAILARLRKQYPRLRTIKILKNKGKAHAFNIGMYFAKGKYILSNDADTIPE
PDALMKYMNFFMHDRDMNTSAVTANMDVQNRTKILGKAQTVEFSSIVGVIKRSQTSINDSMYAYSGANTLYRKSFLIDVG
GFRQNRATEDISIAWDHQMIGAIPRFTPNIVFHMNVPESLKDLYHQRKRWAQGGTEVWLTNIKKFLLHPIKHRYQMSMFI
DSTLSIIWSFFFMITTAVFIITMLVFLLTGNYERVFHGIVMSFIFVTFELLAGFLQLLAALILDHHAAKLKYLIFAPLYM
LFFWMVNPVTVVMTFVPALKTIFGFGSGTWISPERKSLQNK

Sequences:

>Translated_441_residues
MSSIFIDSIFKGSLIDTTINIILLILCLYPVIGAFFWFTGSLSYRFLKTHKRDEGWENILPADQPLITIMIPAHNEEVMI
EETITYLFNEINYTNFEVLVADDGSTDQTGAILARLRKQYPRLRTIKILKNKGKAHAFNIGMYFAKGKYILSNDADTIPE
PDALMKYMNFFMHDRDMNTSAVTANMDVQNRTKILGKAQTVEFSSIVGVIKRSQTSINDSMYAYSGANTLYRKSFLIDVG
GFRQNRATEDISIAWDHQMIGAIPRFTPNIVFHMNVPESLKDLYHQRKRWAQGGTEVWLTNIKKFLLHPIKHRYQMSMFI
DSTLSIIWSFFFMITTAVFIITMLVFLLTGNYERVFHGIVMSFIFVTFELLAGFLQLLAALILDHHAAKLKYLIFAPLYM
LFFWMVNPVTVVMTFVPALKTIFGFGSGTWISPERKSLQNK
>Mature_440_residues
SSIFIDSIFKGSLIDTTINIILLILCLYPVIGAFFWFTGSLSYRFLKTHKRDEGWENILPADQPLITIMIPAHNEEVMIE
ETITYLFNEINYTNFEVLVADDGSTDQTGAILARLRKQYPRLRTIKILKNKGKAHAFNIGMYFAKGKYILSNDADTIPEP
DALMKYMNFFMHDRDMNTSAVTANMDVQNRTKILGKAQTVEFSSIVGVIKRSQTSINDSMYAYSGANTLYRKSFLIDVGG
FRQNRATEDISIAWDHQMIGAIPRFTPNIVFHMNVPESLKDLYHQRKRWAQGGTEVWLTNIKKFLLHPIKHRYQMSMFID
STLSIIWSFFFMITTAVFIITMLVFLLTGNYERVFHGIVMSFIFVTFELLAGFLQLLAALILDHHAAKLKYLIFAPLYML
FFWMVNPVTVVMTFVPALKTIFGFGSGTWISPERKSLQNK

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Homo sapiens, GI7019323, Length=132, Percent_Identity=32.5757575757576, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1787259, Length=418, Percent_Identity=30.8612440191388, Blast_Score=195, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 50699; Mature: 50568

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIFIDSIFKGSLIDTTINIILLILCLYPVIGAFFWFTGSLSYRFLKTHKRDEGWENIL
CCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHCC
PADQPLITIMIPAHNEEVMIEETITYLFNEINYTNFEVLVADDGSTDQTGAILARLRKQY
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHC
PRLRTIKILKNKGKAHAFNIGMYFAKGKYILSNDADTIPEPDALMKYMNFFMHDRDMNTS
CCHHHHHHHHCCCCEEEEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
AVTANMDVQNRTKILGKAQTVEFSSIVGVIKRSQTSINDSMYAYSGANTLYRKSFLIDVG
EEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHEEEEEC
GFRQNRATEDISIAWDHQMIGAIPRFTPNIVFHMNVPESLKDLYHQRKRWAQGGTEVWLT
CCCCCCCCCCCEEEECHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHH
NIKKFLLHPIKHRYQMSMFIDSTLSIIWSFFFMITTAVFIITMLVFLLTGNYERVFHGIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
MSFIFVTFELLAGFLQLLAALILDHHAAKLKYLIFAPLYMLFFWMVNPVTVVMTFVPALK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIFGFGSGTWISPERKSLQNK
HHHCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
SSIFIDSIFKGSLIDTTINIILLILCLYPVIGAFFWFTGSLSYRFLKTHKRDEGWENIL
CCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHCC
PADQPLITIMIPAHNEEVMIEETITYLFNEINYTNFEVLVADDGSTDQTGAILARLRKQY
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHC
PRLRTIKILKNKGKAHAFNIGMYFAKGKYILSNDADTIPEPDALMKYMNFFMHDRDMNTS
CCHHHHHHHHCCCCEEEEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
AVTANMDVQNRTKILGKAQTVEFSSIVGVIKRSQTSINDSMYAYSGANTLYRKSFLIDVG
EEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHEEEEEC
GFRQNRATEDISIAWDHQMIGAIPRFTPNIVFHMNVPESLKDLYHQRKRWAQGGTEVWLT
CCCCCCCCCCCEEEECHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHH
NIKKFLLHPIKHRYQMSMFIDSTLSIIWSFFFMITTAVFIITMLVFLLTGNYERVFHGIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
MSFIFVTFELLAGFLQLLAALILDHHAAKLKYLIFAPLYMLFFWMVNPVTVVMTFVPALK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIFGFGSGTWISPERKSLQNK
HHHCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]