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The map label for this gene is dnaN [H]

Identifier: 116491820

GI number: 116491820

Start: 1568

End: 2707

Strand: Direct

Name: dnaN [H]

Synonym: PEPE_0002

Alternate gene names: 116491820

Gene position: 1568-2707 (Clockwise)

Preceding gene: 116491819

Following gene: 116491821

Centisome position: 0.09

GC content: 35.44

Gene sequence:

>1140_bases
ATGAAATTTACCATCGCTCGAAATACATTTATCAAAAAATTAAATGCGGTTCAAAGAGCCATTTCTTCTAAAACCACGAT
TCCAATTTTGACTGGATTAAAAATTGAAGCAGAAGAAGATCGTTTAATTCTAACTGGAAGCAATACTGACATTTCTATCG
AAACCACCATTTCAACTAACGATGAAGAAGCTCATCTTAATATCGAAAGTACCGGAGCGGTAGTTTTAACAGCCCGTTTC
TTCGGAGATATTGTTAAAAAATTACCAGACAACACTATGTTACTTGAGGTAAAAGACGGTTTTCAAACGACCATTACGTC
AGGTGCTTCTGAATTTAAGATTAATGGTTTAGATGCTAATAACTTCCCTCATTTACCAGAAGTTGATAGTCAAACAACCA
TTACTTTAAAAGCTGATGTATTTAGCGAAATGATTAATGAAACAGTTATCGCAGTTTCTAATCAAGAAAGTCGTCCAATC
TTAACTGGGATTCATTTTACGATTGAAGGAAATAAATTATCAGCCGTAGCAACGGATAGTCATCGTTTGAGCCAACGGGT
AATTAATCTCCCAGAACCAATTAAAATGCCTGAAGATATTATCATCCCTGGGAAGAGTCTTTTAGAACTTTCCAGAATGT
TAGATGATGAAATTGATACAATTACACTTCAAATTTCAGAAAACCAAGCATTATTTACATTTGATGAAACTTTATTCTAT
TCACGTCTATTGGAGGGCTTGTATCCAGATACATCCCGTTTAATTCCAAAGGAAAGTTCAACTGAAATGGAATTTGAAGC
TCCAGAATTGTTAGCTTCGATTGAACGGGCTTCATTGCTTTCACATGCTGGACGCAATAACGTGGTTAAATTAACGATTA
ACGCGGCTAACAAGCAAGCAATCATCTCTGGTGATTCTCCTGAAGTCGGTAATGTAGAAGAAGAAGTGGTTACTAAAGAT
ATTGTGGGTGAAGACCTTGAAATTTCCTTTAACCCAGACTATTTGAAAGATGCGCTACGTTCATTTGGACATACATCAAT
TAAGATGGCCTTTACTTCAGCATTACGTCCATTTACTTTGGTACCAACTGAAGATCAAGAAAACTTTATTCAATTAATCA
CACCTGTTCGAACATTTTAA

Upstream 100 bases:

>100_bases
TTTGAGTTATTAACAGTTCCAACAGCCCTTATTACTACTACTATCTTTTTATTATTAATTAATATATAAAACAGAGAGCA
ACATAAAGGAGTTTTGTAAA

Downstream 100 bases:

>100_bases
TAAAGTAACGCTATGGAGTTTGAGAAAATTAATTTCTCAAACTCTTTTTTTGGATAAATCTGGTTAAAAACGCGTATACG
TAAATTTGAGAAAAAATAAC

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKFTIARNTFIKKLNAVQRAISSKTTIPILTGLKIEAEEDRLILTGSNTDISIETTISTNDEEAHLNIESTGAVVLTARF
FGDIVKKLPDNTMLLEVKDGFQTTITSGASEFKINGLDANNFPHLPEVDSQTTITLKADVFSEMINETVIAVSNQESRPI
LTGIHFTIEGNKLSAVATDSHRLSQRVINLPEPIKMPEDIIIPGKSLLELSRMLDDEIDTITLQISENQALFTFDETLFY
SRLLEGLYPDTSRLIPKESSTEMEFEAPELLASIERASLLSHAGRNNVVKLTINAANKQAIISGDSPEVGNVEEEVVTKD
IVGEDLEISFNPDYLKDALRSFGHTSIKMAFTSALRPFTLVPTEDQENFIQLITPVRTF

Sequences:

>Translated_379_residues
MKFTIARNTFIKKLNAVQRAISSKTTIPILTGLKIEAEEDRLILTGSNTDISIETTISTNDEEAHLNIESTGAVVLTARF
FGDIVKKLPDNTMLLEVKDGFQTTITSGASEFKINGLDANNFPHLPEVDSQTTITLKADVFSEMINETVIAVSNQESRPI
LTGIHFTIEGNKLSAVATDSHRLSQRVINLPEPIKMPEDIIIPGKSLLELSRMLDDEIDTITLQISENQALFTFDETLFY
SRLLEGLYPDTSRLIPKESSTEMEFEAPELLASIERASLLSHAGRNNVVKLTINAANKQAIISGDSPEVGNVEEEVVTKD
IVGEDLEISFNPDYLKDALRSFGHTSIKMAFTSALRPFTLVPTEDQENFIQLITPVRTF
>Mature_379_residues
MKFTIARNTFIKKLNAVQRAISSKTTIPILTGLKIEAEEDRLILTGSNTDISIETTISTNDEEAHLNIESTGAVVLTARF
FGDIVKKLPDNTMLLEVKDGFQTTITSGASEFKINGLDANNFPHLPEVDSQTTITLKADVFSEMINETVIAVSNQESRPI
LTGIHFTIEGNKLSAVATDSHRLSQRVINLPEPIKMPEDIIIPGKSLLELSRMLDDEIDTITLQISENQALFTFDETLFY
SRLLEGLYPDTSRLIPKESSTEMEFEAPELLASIERASLLSHAGRNNVVKLTINAANKQAIISGDSPEVGNVEEEVVTKD
IVGEDLEISFNPDYLKDALRSFGHTSIKMAFTSALRPFTLVPTEDQENFIQLITPVRTF

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=356, Percent_Identity=30.0561797752809, Blast_Score=161, Evalue=5e-41,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41958; Mature: 41958

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTIARNTFIKKLNAVQRAISSKTTIPILTGLKIEAEEDRLILTGSNTDISIETTISTN
CEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCEEEEECCCCCEEEEEEEECC
DEEAHLNIESTGAVVLTARFFGDIVKKLPDNTMLLEVKDGFQTTITSGASEFKINGLDAN
CCCEEEEEECCCEEEEEHHHHHHHHHHCCCCEEEEEECCCCEEHHCCCCCEEEEECCCCC
NFPHLPEVDSQTTITLKADVFSEMINETVIAVSNQESRPILTGIHFTIEGNKLSAVATDS
CCCCCCCCCCCEEEEEEHHHHHHHHCCEEEEEECCCCCCEEEEEEEEEECCEEEEEECCH
HRLSQRVINLPEPIKMPEDIIIPGKSLLELSRMLDDEIDTITLQISENQALFTFDETLFY
HHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCEEEEEEEECCCEEEEEHHHHHH
SRLLEGLYPDTSRLIPKESSTEMEFEAPELLASIERASLLSHAGRNNVVKLTINAANKQA
HHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
IISGDSPEVGNVEEEVVTKDIVGEDLEISFNPDYLKDALRSFGHTSIKMAFTSALRPFTL
EEECCCCCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCCCEEEEEHHHCCCCEEE
VPTEDQENFIQLITPVRTF
ECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFTIARNTFIKKLNAVQRAISSKTTIPILTGLKIEAEEDRLILTGSNTDISIETTISTN
CEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCEEEEECCCCCEEEEEEEECC
DEEAHLNIESTGAVVLTARFFGDIVKKLPDNTMLLEVKDGFQTTITSGASEFKINGLDAN
CCCEEEEEECCCEEEEEHHHHHHHHHHCCCCEEEEEECCCCEEHHCCCCCEEEEECCCCC
NFPHLPEVDSQTTITLKADVFSEMINETVIAVSNQESRPILTGIHFTIEGNKLSAVATDS
CCCCCCCCCCCEEEEEEHHHHHHHHCCEEEEEECCCCCCEEEEEEEEEECCEEEEEECCH
HRLSQRVINLPEPIKMPEDIIIPGKSLLELSRMLDDEIDTITLQISENQALFTFDETLFY
HHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCEEEEEEEECCCEEEEEHHHHHH
SRLLEGLYPDTSRLIPKESSTEMEFEAPELLASIERASLLSHAGRNNVVKLTINAANKQA
HHHHHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
IISGDSPEVGNVEEEVVTKDIVGEDLEISFNPDYLKDALRSFGHTSIKMAFTSALRPFTL
EEECCCCCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCCCEEEEEHHHCCCCEEE
VPTEDQENFIQLITPVRTF
ECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]