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The map label for this gene is cca

Identifier: 116514983

GI number: 116514983

Start: 42863

End: 44107

Strand: Direct

Name: cca

Synonym: BCc_038

Alternate gene names: 116514983

Gene position: 42863-44107 (Clockwise)

Preceding gene: 116514982

Following gene: 116514984

Centisome position: 10.29

GC content: 20.88

Gene sequence:

>1245_bases
TTGAAAATTTATTTAGTTGGTGGAGCTATTCGTGATCGATTATTGAATATTCCTGTTAGAGATCGTGATTGGGTTGTAGT
TGGAATTCGAGATCCTAAAGAAATGTTAAAAAAAAATTATCAACAAGTAGGGAAGGGTTTTCCTGTTTTTATACATCCTA
AAACACACGAAGAATATTCTTTAGCTAGAACTGAAAAAAAAAATGGAGTAGGTCATACAGGTTTTTTATTTGATTTTTCT
TCTAGAATTACCTTAAAAGAAGATTTAAAGCGTAGAGATTTAACTATTAATGCAATAGCACAAGATTCTTCAGGAAAAAT
TATTGATTTTTTTAACGGAAAAAAAGATATTCAAAAAAAAATTTTAAGACATGTATCATATTCATTTCAAGAAGATCCTT
TAAGAGTTTTGCGTATTGCAAGATTTGCAGCTTTATTATCACATTTAGGTTTTTATATTGCGAAAAAAACTCTTTCTTTA
ATGAAATTAATTTGTTCTCGTAAAGAATTATTATATTTAACTCCTGAAAGAATTTGGAAAGAAACGCAGAAAGGATTATC
TACTCAAAATCCTCATGTTTATTTTCAAGTGTTATATTCCTGTAATGCATTGTTTTTTTTATTTCCAGAAATTAATTATT
TTTATAAAAAAACATATTTTTTAAATTCTTTTATTAAACATATTAATTTAGTTCAATATAGTTTAATAGAATTATCAAAA
ATATCTAAAGTTACAAAAGATATTAATATTAGATTTTCTTATTTTTTACAATTTTTTTATTATGTTTATCCTATTCCAAA
TATTGGAACTAAAGATTATTTTTTTTATAAAAAACCTGCTTTTTTATTAAAAAAAATGTTAATTAGATTAAAAATTCCAA
AAGAAACTAGTGAAATTATTTTTTTTTTATGTGGTTTTCACAATTTTTTACAAAATATTAATATACAAAGTTCAAAGTTA
ATAATAAAATTTTTTAATATTATTGATGTATGGAGAAAACCCAATAGATTAAATCAGTTAATATATTTAGATTTTTATAA
TTTTAATTCTTTGAAAAATAAAAAGAATGATTTTTTTTTAGGTAAGTTATTAAAATATATGTTTTCTTTAATTAAAGATA
TTTCTGTTTGTTCTTTCATAAAAGAAAAGAAATTTAAAGGTATTGAAATAAAAAATGATTTAAATCGTTTAAGAATACAA
TCTTTTAAAGAAATGAAGGAAAAAATAATATTAAATATATTTTAA

Upstream 100 bases:

>100_bases
AATATTTTTAAATATATTATTATATTTTTAATGTTACATAAAATTTTTAAATATTTATTTTTAAAAAAAATTTTTATTTT
TTTATTATTTAGGTATTATT

Downstream 100 bases:

>100_bases
AATATATTATAGATTAAATTTTAATTAATATAAATTCATTATAAATAAAATAATTTTGTTATTCCAGCTTTTATTTTATA
TTTTTTTTTATAAGTGTTAA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKIYLVGGAIRDRLLNIPVRDRDWVVVGIRDPKEMLKKNYQQVGKGFPVFIHPKTHEEYSLARTEKKNGVGHTGFLFDFS
SRITLKEDLKRRDLTINAIAQDSSGKIIDFFNGKKDIQKKILRHVSYSFQEDPLRVLRIARFAALLSHLGFYIAKKTLSL
MKLICSRKELLYLTPERIWKETQKGLSTQNPHVYFQVLYSCNALFFLFPEINYFYKKTYFLNSFIKHINLVQYSLIELSK
ISKVTKDINIRFSYFLQFFYYVYPIPNIGTKDYFFYKKPAFLLKKMLIRLKIPKETSEIIFFLCGFHNFLQNINIQSSKL
IIKFFNIIDVWRKPNRLNQLIYLDFYNFNSLKNKKNDFFLGKLLKYMFSLIKDISVCSFIKEKKFKGIEIKNDLNRLRIQ
SFKEMKEKIILNIF

Sequences:

>Translated_414_residues
MKIYLVGGAIRDRLLNIPVRDRDWVVVGIRDPKEMLKKNYQQVGKGFPVFIHPKTHEEYSLARTEKKNGVGHTGFLFDFS
SRITLKEDLKRRDLTINAIAQDSSGKIIDFFNGKKDIQKKILRHVSYSFQEDPLRVLRIARFAALLSHLGFYIAKKTLSL
MKLICSRKELLYLTPERIWKETQKGLSTQNPHVYFQVLYSCNALFFLFPEINYFYKKTYFLNSFIKHINLVQYSLIELSK
ISKVTKDINIRFSYFLQFFYYVYPIPNIGTKDYFFYKKPAFLLKKMLIRLKIPKETSEIIFFLCGFHNFLQNINIQSSKL
IIKFFNIIDVWRKPNRLNQLIYLDFYNFNSLKNKKNDFFLGKLLKYMFSLIKDISVCSFIKEKKFKGIEIKNDLNRLRIQ
SFKEMKEKIILNIF
>Mature_414_residues
MKIYLVGGAIRDRLLNIPVRDRDWVVVGIRDPKEMLKKNYQQVGKGFPVFIHPKTHEEYSLARTEKKNGVGHTGFLFDFS
SRITLKEDLKRRDLTINAIAQDSSGKIIDFFNGKKDIQKKILRHVSYSFQEDPLRVLRIARFAALLSHLGFYIAKKTLSL
MKLICSRKELLYLTPERIWKETQKGLSTQNPHVYFQVLYSCNALFFLFPEINYFYKKTYFLNSFIKHINLVQYSLIELSK
ISKVTKDINIRFSYFLQFFYYVYPIPNIGTKDYFFYKKPAFLLKKMLIRLKIPKETSEIIFFLCGFHNFLQNINIQSSKL
IIKFFNIIDVWRKPNRLNQLIYLDFYNFNSLKNKKNDFFLGKLLKYMFSLIKDISVCSFIKEKKFKGIEIKNDLNRLRIQ
SFKEMKEKIILNIF

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=409, Percent_Identity=43.2762836185819, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI87081691, Length=232, Percent_Identity=27.1551724137931, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI281360112, Length=213, Percent_Identity=28.6384976525822, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21357337, Length=213, Percent_Identity=28.6384976525822, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_BUCCC (Q058D0)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802612.1
- ProteinModelPortal:   Q058D0
- SMR:   Q058D0
- STRING:   Q058D0
- EnsemblBacteria:   EBBUCT00000001432
- GeneID:   4440713
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_038
- eggNOG:   COG0617
- GeneTree:   EBGT00050000008009
- HOGENOM:   HBG635063
- OMA:   QVGKGFP
- ProtClustDB:   PRK13298
- BioCyc:   BAPH372461:BCC_038-MONOMER
- HAMAP:   MF_01262
- InterPro:   IPR012006
- InterPro:   IPR002646
- PIRSF:   PIRSF000813

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 49357; Mature: 49357

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 92-92 BINDING 138-138 BINDING 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLVGGAIRDRLLNIPVRDRDWVVVGIRDPKEMLKKNYQQVGKGFPVFIHPKTHEEYS
CEEEEECHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCHHH
LARTEKKNGVGHTGFLFDFSSRITLKEDLKRRDLTINAIAQDSSGKIIDFFNGKKDIQKK
HHHHHHCCCCCCCEEEEECCCCCCHHHHHHHCCCEEEEEEECCCCCEEEECCCHHHHHHH
ILRHVSYSFQEDPLRVLRIARFAALLSHLGFYIAKKTLSLMKLICSRKELLYLTPERIWK
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHH
ETQKGLSTQNPHVYFQVLYSCNALFFLFPEINYFYKKTYFLNSFIKHINLVQYSLIELSK
HHHHCCCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISKVTKDINIRFSYFLQFFYYVYPIPNIGTKDYFFYKKPAFLLKKMLIRLKIPKETSEII
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHEECCCCHHHHH
FFLCGFHNFLQNINIQSSKLIIKFFNIIDVWRKPNRLNQLIYLDFYNFNSLKNKKNDFFL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCHHH
GKLLKYMFSLIKDISVCSFIKEKKFKGIEIKNDLNRLRIQSFKEMKEKIILNIF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHEEECC
>Mature Secondary Structure
MKIYLVGGAIRDRLLNIPVRDRDWVVVGIRDPKEMLKKNYQQVGKGFPVFIHPKTHEEYS
CEEEEECHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCHHH
LARTEKKNGVGHTGFLFDFSSRITLKEDLKRRDLTINAIAQDSSGKIIDFFNGKKDIQKK
HHHHHHCCCCCCCEEEEECCCCCCHHHHHHHCCCEEEEEEECCCCCEEEECCCHHHHHHH
ILRHVSYSFQEDPLRVLRIARFAALLSHLGFYIAKKTLSLMKLICSRKELLYLTPERIWK
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHH
ETQKGLSTQNPHVYFQVLYSCNALFFLFPEINYFYKKTYFLNSFIKHINLVQYSLIELSK
HHHHCCCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISKVTKDINIRFSYFLQFFYYVYPIPNIGTKDYFFYKKPAFLLKKMLIRLKIPKETSEII
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHEECCCCHHHHH
FFLCGFHNFLQNINIQSSKLIIKFFNIIDVWRKPNRLNQLIYLDFYNFNSLKNKKNDFFL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCHHH
GKLLKYMFSLIKDISVCSFIKEKKFKGIEIKNDLNRLRIQSFKEMKEKIILNIF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA