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Identifier: 116514984

GI number: 116514984

Start: 44744

End: 46507

Strand: Direct

Name: lig

Synonym: BCc_039

Alternate gene names: 116514984

Gene position: 44744-46507 (Clockwise)

Preceding gene: 116514983

Following gene: 116514985

Centisome position: 10.75

GC content: 20.24

Gene sequence:

>1764_bases
ATGAAAGATATCTATAATCAAATTTTAAAATTACAATTACAAATTAAATATCATAATTACATGTATCATACATTAGATTC
TCCTATTATTTCAGATATTTTATATGATGAATTATATAATAAATTACTTCAATTAGAAAAATTATATTTTAAAAAAAAAT
CTTTAGATAAAAAAATAAAATTATTAGATCAAGTAGGAGCGAAAAAATTACATATTTTTACTGAATTTTTTCATAAAATT
CCTATGCTTTCTTTAAGAAGTATAAATAATATTTCTGATTTTGATTTATTTGATAAAAAAATAAAGGAATATTTTAAACA
TATTAATGTTATTACATATTTTTGTGATTTTAAATTTGATGGATTAGCTGTTAATTTATTTTATAAAAATGGAATTTTAA
TTTCTGCTTCTACTCGTGGAAATGGTAGTGTCGGGGAAAATATTACAAAAAATATTTTAATGATTTCTTCAATTCCAAAA
AAAATTGCAGGAAGCAATATTCCTAAAAAAATAGAAATACGTGGAGAAATATTTATGAGAAAATCTGATTTTTTTATTTT
AAATCAATCTTGTAAATTAAGTGGAAAAAAAGAATTTTCTAATCCTCGTAATGCTGCTGCAGGCTCTGTTCGTCAGTTAA
ATCCAGAAATTGTAAAAAAAAGAAAGTTAAATTTTTTCGTGTATGGATTTGGATTATTTGATTATAATAAAAAAATTGAT
AGTCATTATCAAAGATTATTACAAATAAAAAAATGGGGTTTTCCATTATATAAAAATTATTGTGTATGTAAAAATAAAAA
AGAAGTAATTCATTTTTATCATTATGCAAATAAAATTAGATCACAATTAGATTTTGAAATTGACGGGATAGTTATTAAAC
TAGATTCTATTAAATTACAAAATAATTTAGGTTGTATAGAAAAATATCCTAAATGGGCAATTGCATTAAAGTTTTTTTCT
TTAGATAAAGAAACTAAAATTTTTAAAATATCATTTAAAGTTGGAAGAACGGGTATTATTACTCCTGTTGCTTATTTTTT
TCCAATTAATCTTTTTGGTGTTTCTATAAGTAAAGCTTCATTATATAATATAAAAACAATAAAATTATTAGATATACGTC
TTCATGATTATGTTACTGTATACCGTGCTGGTGATGTAATTCCGAAAATTAGAAATGTTTTAATACATAAAAGAAATAAA
TATACACAGAAGATAATTATTCCAACATACTGTCCTTCTTGTTGTACTAAATTGATTTTTTCTGATGATTTAAAAACATG
TTATTGTCCTGCTAGTTTTTCTTGTTTTTCACAAAATGTTAAAAGATTAATTTATTTTTCTTCAAAAAATGGTTTAAATT
TTAAAGGTTTAGGAAAAAAAAATATTATTAAATTAATAAATTATGGTTATTTATATACTCCTATTGATTTTTTTTCTTTA
ACAGTAAAGAAATTAAAAAATATATTTCGTATGGGAGACAAATTATCTGAAAAAATTATTAAAAATATTGCTTTTTCTAA
AAGAGTTTCTTTAGATAAATTTATTTGTTCGTTAGGAATTTTTGGTGTTGGAACATCAATTGCTAAACGTTTAGCATATT
ATTATCGATCTGTAGAAAAATTTTTTAATACTAATTATGATACATTATCTAAAATTGATCATATTGGATATAATATTTCT
AATGCAATTATTAGTTTTTTAAAAAATAAAAGTAATAGATCAATTATTTTAAAATTAATTAGAATACTAAATATTTTTAT
TTAA

Upstream 100 bases:

>100_bases
AGATTATATTGTCTTTTTTATTTTCAATTATTAAAAATATTATTTATTATATTTAATAATTGTTTTTATTAAATTTAAAT
TATTAAATATGGTGTTTATT

Downstream 100 bases:

>100_bases
TTTTATAAAAATATATTATTAAAAGTTTTGGGTCGTGCAGGATTTGAACCTGCGACCAATTGATTAAAAGTCAACTGCTC
TACCAACTGAGCTAACGACC

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MKDIYNQILKLQLQIKYHNYMYHTLDSPIISDILYDELYNKLLQLEKLYFKKKSLDKKIKLLDQVGAKKLHIFTEFFHKI
PMLSLRSINNISDFDLFDKKIKEYFKHINVITYFCDFKFDGLAVNLFYKNGILISASTRGNGSVGENITKNILMISSIPK
KIAGSNIPKKIEIRGEIFMRKSDFFILNQSCKLSGKKEFSNPRNAAAGSVRQLNPEIVKKRKLNFFVYGFGLFDYNKKID
SHYQRLLQIKKWGFPLYKNYCVCKNKKEVIHFYHYANKIRSQLDFEIDGIVIKLDSIKLQNNLGCIEKYPKWAIALKFFS
LDKETKIFKISFKVGRTGIITPVAYFFPINLFGVSISKASLYNIKTIKLLDIRLHDYVTVYRAGDVIPKIRNVLIHKRNK
YTQKIIIPTYCPSCCTKLIFSDDLKTCYCPASFSCFSQNVKRLIYFSSKNGLNFKGLGKKNIIKLINYGYLYTPIDFFSL
TVKKLKNIFRMGDKLSEKIIKNIAFSKRVSLDKFICSLGIFGVGTSIAKRLAYYYRSVEKFFNTNYDTLSKIDHIGYNIS
NAIISFLKNKSNRSIILKLIRILNIFI

Sequences:

>Translated_587_residues
MKDIYNQILKLQLQIKYHNYMYHTLDSPIISDILYDELYNKLLQLEKLYFKKKSLDKKIKLLDQVGAKKLHIFTEFFHKI
PMLSLRSINNISDFDLFDKKIKEYFKHINVITYFCDFKFDGLAVNLFYKNGILISASTRGNGSVGENITKNILMISSIPK
KIAGSNIPKKIEIRGEIFMRKSDFFILNQSCKLSGKKEFSNPRNAAAGSVRQLNPEIVKKRKLNFFVYGFGLFDYNKKID
SHYQRLLQIKKWGFPLYKNYCVCKNKKEVIHFYHYANKIRSQLDFEIDGIVIKLDSIKLQNNLGCIEKYPKWAIALKFFS
LDKETKIFKISFKVGRTGIITPVAYFFPINLFGVSISKASLYNIKTIKLLDIRLHDYVTVYRAGDVIPKIRNVLIHKRNK
YTQKIIIPTYCPSCCTKLIFSDDLKTCYCPASFSCFSQNVKRLIYFSSKNGLNFKGLGKKNIIKLINYGYLYTPIDFFSL
TVKKLKNIFRMGDKLSEKIIKNIAFSKRVSLDKFICSLGIFGVGTSIAKRLAYYYRSVEKFFNTNYDTLSKIDHIGYNIS
NAIISFLKNKSNRSIILKLIRILNIFI
>Mature_587_residues
MKDIYNQILKLQLQIKYHNYMYHTLDSPIISDILYDELYNKLLQLEKLYFKKKSLDKKIKLLDQVGAKKLHIFTEFFHKI
PMLSLRSINNISDFDLFDKKIKEYFKHINVITYFCDFKFDGLAVNLFYKNGILISASTRGNGSVGENITKNILMISSIPK
KIAGSNIPKKIEIRGEIFMRKSDFFILNQSCKLSGKKEFSNPRNAAAGSVRQLNPEIVKKRKLNFFVYGFGLFDYNKKID
SHYQRLLQIKKWGFPLYKNYCVCKNKKEVIHFYHYANKIRSQLDFEIDGIVIKLDSIKLQNNLGCIEKYPKWAIALKFFS
LDKETKIFKISFKVGRTGIITPVAYFFPINLFGVSISKASLYNIKTIKLLDIRLHDYVTVYRAGDVIPKIRNVLIHKRNK
YTQKIIIPTYCPSCCTKLIFSDDLKTCYCPASFSCFSQNVKRLIYFSSKNGLNFKGLGKKNIIKLINYGYLYTPIDFFSL
TVKKLKNIFRMGDKLSEKIIKNIAFSKRVSLDKFICSLGIFGVGTSIAKRLAYYYRSVEKFFNTNYDTLSKIDHIGYNIS
NAIISFLKNKSNRSIILKLIRILNIFI

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigA subfamily

Homologues:

Organism=Escherichia coli, GI1788750, Length=580, Percent_Identity=37.5862068965517, Blast_Score=493, Evalue=1e-140,
Organism=Escherichia coli, GI87082305, Length=570, Percent_Identity=22.6315789473684, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_BUCCC (Q058C9)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802613.1
- ProteinModelPortal:   Q058C9
- SMR:   Q058C9
- STRING:   Q058C9
- EnsemblBacteria:   EBBUCT00000001514
- GeneID:   4440714
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_039
- eggNOG:   COG0272
- GeneTree:   EBGT00050000007874
- HOGENOM:   HBG620317
- OMA:   FHELKAL
- BioCyc:   BAPH372461:BCC_039-MONOMER
- HAMAP:   MF_01588
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 68401; Mature: 68401

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 118-118 BINDING 116-116 BINDING 139-139 BINDING 176-176 BINDING 293-293 BINDING 317-317

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIYNQILKLQLQIKYHNYMYHTLDSPIISDILYDELYNKLLQLEKLYFKKKSLDKKIK
CHHHHHHHHEEEEEEEEECEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLDQVGAKKLHIFTEFFHKIPMLSLRSINNISDFDLFDKKIKEYFKHINVITYFCDFKFD
HHHHCCCHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCC
GLAVNLFYKNGILISASTRGNGSVGENITKNILMISSIPKKIAGSNIPKKIEIRGEIFMR
CEEEEEEEECCEEEEECCCCCCCCCCCHHHHEEEEHHCCHHHCCCCCCCEEEEEEEEEEE
KSDFFILNQSCKLSGKKEFSNPRNAAAGSVRQLNPEIVKKRKLNFFVYGFGLFDYNKKID
ECCEEEEECCCCCCCCHHCCCCCCHHCCCHHHCCHHHHHHHCCEEEEEEEEEECCCHHHH
SHYQRLLQIKKWGFPLYKNYCVCKNKKEVIHFYHYANKIRSQLDFEIDGIVIKLDSIKLQ
HHHHHHHHHHHCCCCHHCCEEEECCCHHHHHHHHHHHHHHHHCCEEECEEEEEEEEEEEC
NNLGCIEKYPKWAIALKFFSLDKETKIFKISFKVGRTGIITPVAYFFPINLFGVSISKAS
CCCCHHHHCCCCEEEEEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHEEEEECCCE
LYNIKTIKLLDIRLHDYVTVYRAGDVIPKIRNVLIHKRNKYTQKIIIPTYCPSCCTKLIF
EEEEEEEEEEEEEEHHEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHH
SDDLKTCYCPASFSCFSQNVKRLIYFSSKNGLNFKGLGKKNIIKLINYGYLYTPIDFFSL
CCCCCEEECCCCHHHHHHCHHEEEEEECCCCCCCCCCCHHHHHHHHHCCEEECCHHHHHH
TVKKLKNIFRMGDKLSEKIIKNIAFSKRVSLDKFICSLGIFGVGTSIAKRLAYYYRSVEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
FFNTNYDTLSKIDHIGYNISNAIISFLKNKSNRSIILKLIRILNIFI
HHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDIYNQILKLQLQIKYHNYMYHTLDSPIISDILYDELYNKLLQLEKLYFKKKSLDKKIK
CHHHHHHHHEEEEEEEEECEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLDQVGAKKLHIFTEFFHKIPMLSLRSINNISDFDLFDKKIKEYFKHINVITYFCDFKFD
HHHHCCCHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCC
GLAVNLFYKNGILISASTRGNGSVGENITKNILMISSIPKKIAGSNIPKKIEIRGEIFMR
CEEEEEEEECCEEEEECCCCCCCCCCCHHHHEEEEHHCCHHHCCCCCCCEEEEEEEEEEE
KSDFFILNQSCKLSGKKEFSNPRNAAAGSVRQLNPEIVKKRKLNFFVYGFGLFDYNKKID
ECCEEEEECCCCCCCCHHCCCCCCHHCCCHHHCCHHHHHHHCCEEEEEEEEEECCCHHHH
SHYQRLLQIKKWGFPLYKNYCVCKNKKEVIHFYHYANKIRSQLDFEIDGIVIKLDSIKLQ
HHHHHHHHHHHCCCCHHCCEEEECCCHHHHHHHHHHHHHHHHCCEEECEEEEEEEEEEEC
NNLGCIEKYPKWAIALKFFSLDKETKIFKISFKVGRTGIITPVAYFFPINLFGVSISKAS
CCCCHHHHCCCCEEEEEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHEEEEECCCE
LYNIKTIKLLDIRLHDYVTVYRAGDVIPKIRNVLIHKRNKYTQKIIIPTYCPSCCTKLIF
EEEEEEEEEEEEEEHHEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHH
SDDLKTCYCPASFSCFSQNVKRLIYFSSKNGLNFKGLGKKNIIKLINYGYLYTPIDFFSL
CCCCCEEECCCCHHHHHHCHHEEEEEECCCCCCCCCCCHHHHHHHHHCCEEECCHHHHHH
TVKKLKNIFRMGDKLSEKIIKNIAFSKRVSLDKFICSLGIFGVGTSIAKRLAYYYRSVEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
FFNTNYDTLSKIDHIGYNISNAIISFLKNKSNRSIILKLIRILNIFI
HHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA