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The map label for this gene is rpoH [H]

Identifier: 116514965

GI number: 116514965

Start: 16181

End: 17056

Strand: Direct

Name: rpoH [H]

Synonym: BCc_016

Alternate gene names: 116514965

Gene position: 16181-17056 (Clockwise)

Preceding gene: 116514964

Following gene: 116514966

Centisome position: 3.89

GC content: 25.11

Gene sequence:

>876_bases
ATGAAAAAATTAGAAAGTCAAATTAATATGATTGGTTTTTTTAATATTGGAAGTTTAGAAGCGTATATTCGATTAGCAAA
TTCTTTTTCTATTTTAACATCAGAAAAAGAAAAATCTCTTGCTAAAAAATTATTTTTTTATAGTGATATAGAATCAGCAA
AATTATTAATTTTATCAAATTTACGATTTGTAATTCATGTAGCAAAAAATTATTCAGGATATGGTTTGCCACAAGCAGAT
TTAATTCAAGAAGGAAATATTGGATTAATGAAAGCTATTAGACGTTTTAACCCTAATATTAATGTTCGATTAGTTTCTTT
TGCTGTACATTGGATAAAATCAGAAATTCATGAATATGTTTTGAGAAATTGGAGAATTGTAAAAATTGCAACAACTAAAG
CTCAAAGAAAATTATTTTTTAATTTAAGAAAATCAAAAAAAAGATTAGGTTGGTTTAATCGTCATGAAATAGATACAGTT
TCTCAGGAATTAGGAGTAAGTACTCAAGAAGTAAAAGAAATGGAAGCTCGAATGTCTGCTCAAGATATTACTTTTGATCT
TTCTGCTAAAGATTCTCATCTTGATTCTATTACAAATAATTTACGTACTTCTTTTCATTTAGAAGATAAAAAATCTAATT
TTTCTATAAAAATAGAAAAAGATAATTGGGCATTACATGCCACGAATAAACTTACTAACGCATTATTAATTTTAGATAAA
AGAAGTCAACAAATTATTAAAAGACGATGGTTAGATGATGATAAACCTAAAATTACATTACAAGCAATTGCTAGAGATTA
TGGAATTTCTGCAGAAAGAGTAAGACAATTAGAAAAAAATGCTATGAAAAAATTAAAAATTGCTATTGAAGCATAG

Upstream 100 bases:

>100_bases
CATTGAGAATTTTTATAATATTTTTTAAAAAATGATATAATTATTATCTTTTAGATAATTTTTTTTTTATTAAAATAAAA
TATTTTATATTTAGGAAAAA

Downstream 100 bases:

>100_bases
AATATGAATTATTTTAGTTAATAACATTAAATATTTCTAAATTTTTTTTAAAAAACACCAGAAATATTTTTAAATAAAAA
ACAAATAGGTTGAAATAATA

Product: RNA polymerase sigma-32 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKKLESQINMIGFFNIGSLEAYIRLANSFSILTSEKEKSLAKKLFFYSDIESAKLLILSNLRFVIHVAKNYSGYGLPQAD
LIQEGNIGLMKAIRRFNPNINVRLVSFAVHWIKSEIHEYVLRNWRIVKIATTKAQRKLFFNLRKSKKRLGWFNRHEIDTV
SQELGVSTQEVKEMEARMSAQDITFDLSAKDSHLDSITNNLRTSFHLEDKKSNFSIKIEKDNWALHATNKLTNALLILDK
RSQQIIKRRWLDDDKPKITLQAIARDYGISAERVRQLEKNAMKKLKIAIEA

Sequences:

>Translated_291_residues
MKKLESQINMIGFFNIGSLEAYIRLANSFSILTSEKEKSLAKKLFFYSDIESAKLLILSNLRFVIHVAKNYSGYGLPQAD
LIQEGNIGLMKAIRRFNPNINVRLVSFAVHWIKSEIHEYVLRNWRIVKIATTKAQRKLFFNLRKSKKRLGWFNRHEIDTV
SQELGVSTQEVKEMEARMSAQDITFDLSAKDSHLDSITNNLRTSFHLEDKKSNFSIKIEKDNWALHATNKLTNALLILDK
RSQQIIKRRWLDDDKPKITLQAIARDYGISAERVRQLEKNAMKKLKIAIEA
>Mature_291_residues
MKKLESQINMIGFFNIGSLEAYIRLANSFSILTSEKEKSLAKKLFFYSDIESAKLLILSNLRFVIHVAKNYSGYGLPQAD
LIQEGNIGLMKAIRRFNPNINVRLVSFAVHWIKSEIHEYVLRNWRIVKIATTKAQRKLFFNLRKSKKRLGWFNRHEIDTV
SQELGVSTQEVKEMEARMSAQDITFDLSAKDSHLDSITNNLRTSFHLEDKKSNFSIKIEKDNWALHATNKLTNALLILDK
RSQQIIKRRWLDDDKPKITLQAIARDYGISAERVRQLEKNAMKKLKIAIEA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=288, Percent_Identity=62.1527777777778, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1789098, Length=262, Percent_Identity=25.1908396946565, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789448, Length=235, Percent_Identity=25.531914893617, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33754; Mature: 33754

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLESQINMIGFFNIGSLEAYIRLANSFSILTSEKEKSLAKKLFFYSDIESAKLLILSN
CCCHHHHCCEEEEEECCCHHHHHHHHCCHHEEECHHHHHHHHHHHHHCCCCCCEEEEECC
LRFVIHVAKNYSGYGLPQADLIQEGNIGLMKAIRRFNPNINVRLVSFAVHWIKSEIHEYV
CEEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
LRNWRIVKIATTKAQRKLFFNLRKSKKRLGWFNRHEIDTVSQELGVSTQEVKEMEARMSA
HHCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCH
QDITFDLSAKDSHLDSITNNLRTSFHLEDKKSNFSIKIEKDNWALHATNKLTNALLILDK
HHEEEEECCCHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCEEEEECHHHCEEEEEEEC
RSQQIIKRRWLDDDKPKITLQAIARDYGISAERVRQLEKNAMKKLKIAIEA
HHHHHHHHHCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MKKLESQINMIGFFNIGSLEAYIRLANSFSILTSEKEKSLAKKLFFYSDIESAKLLILSN
CCCHHHHCCEEEEEECCCHHHHHHHHCCHHEEECHHHHHHHHHHHHHCCCCCCEEEEECC
LRFVIHVAKNYSGYGLPQADLIQEGNIGLMKAIRRFNPNINVRLVSFAVHWIKSEIHEYV
CEEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
LRNWRIVKIATTKAQRKLFFNLRKSKKRLGWFNRHEIDTVSQELGVSTQEVKEMEARMSA
HHCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCH
QDITFDLSAKDSHLDSITNNLRTSFHLEDKKSNFSIKIEKDNWALHATNKLTNALLILDK
HHEEEEECCCHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCEEEEECHHHCEEEEEEEC
RSQQIIKRRWLDDDKPKITLQAIARDYGISAERVRQLEKNAMKKLKIAIEA
HHHHHHHHHCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]