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The map label for this gene is metE

Identifier: 116514966

GI number: 116514966

Start: 17328

End: 19610

Strand: Direct

Name: metE

Synonym: BCc_017

Alternate gene names: 116514966

Gene position: 17328-19610 (Clockwise)

Preceding gene: 116514965

Following gene: 116514975

Centisome position: 4.16

GC content: 24.09

Gene sequence:

>2283_bases
ATGGCTATAAAAAATCATATACTAGGATTTCCTAGAATTGGTTTAAATCGTGAATTAAAATTTGCTTTAGAAAAATACTG
GTCAAAAAAAAATACTTTAGAAGAATTACTTTTGATAGGTAAAAATATTAGAAAAGAAAATTGGAAAAATCAAATTGATA
GTGGAATGGACTATGTTACTGTAGGTGATTTTGCATGGTATGATCATGTATTAAATATTAGCATGATGATAAATAATATA
CCAGAAAGACATAATCCTAATAATCATATTTTAAATATTGATACTTTATTTAAAGTAGCTAGAGGATCTAAAGGAGTAGA
TAATAATATTTGTTCAGCTTCAGAAATGACTAAGTGGTTTAATACAAATTATCATTATATAGTGCCTGAATTTACTTCTA
ATCAAAAATTTTATTTTGCTTGGAAACAAATTTTAGAAGAAACTGATGAAGCATTATCTTTAGGATATAAAGTAAAACCA
GTTTTATTAGGTCCATTAACATATTTATGGTTAGGAAAAGTAAAAAATAGTAAAATAAATAAATTAGATTTATTAAAAAA
AATATTACCTATTTATATCCAAGTATTTAAAGAATTATCTTCTAGAAATATTAATTGGATTCAAATTGATGAACCTATTT
TAGTATTAGATATCCCTAAAAATTGGAAAAAAGAATTTCAATCGACTTATAAATTTTTAGATGGTAAAATAAAAATTTTA
TTAGCTACTTATTTTGGTGATATCACTCATAATTTAGATATTATTAATAAATTATCTATTCAAGGATTGCATATTGATTT
AGTATCTAGTAAATATGATTTACTTAAATTGTCAAAATCAATTAATAATAATTTTTTATTATCATTAGGTATAATTAATG
GAAGGAATATTTGGAAAACAAATCTTTTAGAATGGTTTTATAAGTTAAAAGATTTTATGAAAATAAATAATAATTTTTGG
ATAAGTTCTTCATGTTCTTTATTGCATGTTCCTTTAGATATCACTATTGAAGAAAATTTAACTGATTTTGTAAAATCATG
GTTTTCTTTTGGTATTCAAAAATGTTTAGAAATTTCTTTATTATCTCAAGTTTTACAAAACAATTTAGATATCAAAGAAT
TAAAAAATTGGATTAAACCAATTCATGAATATAAATCTTCTAATATAGTTAATAATACTTCTGTACAAAAACGTACATTA
AAAATTTCTTCAGAACAATTTATTAGAAAAAATGAATTTTCTGTTCGTTCTAAAATTCAAAAAGAAACATTATGTTTGCC
TGTACTTCCTACTACTACTATTGGTTCTTTTCCTCAAACTTCAGAAATTAGAAAATTAAGATTAGATTATAAAAATAAAA
AAATTAATCAATTAGACTATGAAAAACAAATTAAAATACACATAAAAAAAAATATTATTCAACAAGAACAATTAGGATTA
GATGTTTTAGTTCATGGTGAACCTGAACGTAATGATATGGTAGAATATTTTAGTGAATATTTAGAAGGTTTTGTATTTAC
AACATATGGATGGGTACAAAGTTACGGTTCACGTTGTGTTAAACCGCCAATTATTGTAGGAGATATTAGTAGAATTACTC
CTATGACAGTTATGTGGTCTAAATATGCTCAATCTTTAACTAAAAAACCAGTTAAAGCAATGTTAACAGGACCAGTAACA
ATTTTATGTTGGTCTTTTCCTAGAGAAGATATTTCTAAAGAAGATATATGTAATCAAATTGCAATATCTTTAAGAGATGA
AGTGTTAGATTTAGAAAATTCTGGTATTAATATTATTCAAATTGATGAACCAGCATTACGAGAAGGTTTACCATTAAGAA
CTCATGAATGGAATAATTATTTACGATGGGCTGTAAAATCATTTAAGATATGCTCGTCTGGAGTTAAAAATAGTACTCAA
ATTCATACTCATATGTGTTATTGTGAGTTTAATGATATTATGCCGGCTATTGTAGATTTAGATGCAGATGTGATTACTAT
TGAAACATCTAGATCAGATATGGAATTATTAGAATTTTTTAAAACATTTAAATATCCAAATGCTATTGGTCCAGGAGTAT
ATGATATTCATTCTCCAAATATACCATCTGTTCAATCGATAGAGAAATTACTAAAAAAAGCATTGAAATATATTTCAATT
CAACAATTATGGGTAAATCCTGATTGTGGTTTAAAAACTCGTAATTGGACAGAGACATCTCTTGCATTACAAAATATGCT
ACAAGCAACTTTAAATATTAGAAAAGAATATTTTAAAAAATAA

Upstream 100 bases:

>100_bases
TACATTATCACACTTTTAAAGTATAAATATATGATTATTATATTTAATATATCTTTTAAATAATTAATTATTATGAATAC
AAAAATAGGAAAAAATATAT

Downstream 100 bases:

>100_bases
AAAATTTATTTTTTAATCCACTTTATGCAATCGAAAATTTTCTTGATTGTGTTTAGTGGTTATTAAAAATAAAATATTTT
TTTATAAAAAATTAATAATT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 760; Mature: 759

Protein sequence:

>760_residues
MAIKNHILGFPRIGLNRELKFALEKYWSKKNTLEELLLIGKNIRKENWKNQIDSGMDYVTVGDFAWYDHVLNISMMINNI
PERHNPNNHILNIDTLFKVARGSKGVDNNICSASEMTKWFNTNYHYIVPEFTSNQKFYFAWKQILEETDEALSLGYKVKP
VLLGPLTYLWLGKVKNSKINKLDLLKKILPIYIQVFKELSSRNINWIQIDEPILVLDIPKNWKKEFQSTYKFLDGKIKIL
LATYFGDITHNLDIINKLSIQGLHIDLVSSKYDLLKLSKSINNNFLLSLGIINGRNIWKTNLLEWFYKLKDFMKINNNFW
ISSSCSLLHVPLDITIEENLTDFVKSWFSFGIQKCLEISLLSQVLQNNLDIKELKNWIKPIHEYKSSNIVNNTSVQKRTL
KISSEQFIRKNEFSVRSKIQKETLCLPVLPTTTIGSFPQTSEIRKLRLDYKNKKINQLDYEKQIKIHIKKNIIQQEQLGL
DVLVHGEPERNDMVEYFSEYLEGFVFTTYGWVQSYGSRCVKPPIIVGDISRITPMTVMWSKYAQSLTKKPVKAMLTGPVT
ILCWSFPREDISKEDICNQIAISLRDEVLDLENSGINIIQIDEPALREGLPLRTHEWNNYLRWAVKSFKICSSGVKNSTQ
IHTHMCYCEFNDIMPAIVDLDADVITIETSRSDMELLEFFKTFKYPNAIGPGVYDIHSPNIPSVQSIEKLLKKALKYISI
QQLWVNPDCGLKTRNWTETSLALQNMLQATLNIRKEYFKK

Sequences:

>Translated_760_residues
MAIKNHILGFPRIGLNRELKFALEKYWSKKNTLEELLLIGKNIRKENWKNQIDSGMDYVTVGDFAWYDHVLNISMMINNI
PERHNPNNHILNIDTLFKVARGSKGVDNNICSASEMTKWFNTNYHYIVPEFTSNQKFYFAWKQILEETDEALSLGYKVKP
VLLGPLTYLWLGKVKNSKINKLDLLKKILPIYIQVFKELSSRNINWIQIDEPILVLDIPKNWKKEFQSTYKFLDGKIKIL
LATYFGDITHNLDIINKLSIQGLHIDLVSSKYDLLKLSKSINNNFLLSLGIINGRNIWKTNLLEWFYKLKDFMKINNNFW
ISSSCSLLHVPLDITIEENLTDFVKSWFSFGIQKCLEISLLSQVLQNNLDIKELKNWIKPIHEYKSSNIVNNTSVQKRTL
KISSEQFIRKNEFSVRSKIQKETLCLPVLPTTTIGSFPQTSEIRKLRLDYKNKKINQLDYEKQIKIHIKKNIIQQEQLGL
DVLVHGEPERNDMVEYFSEYLEGFVFTTYGWVQSYGSRCVKPPIIVGDISRITPMTVMWSKYAQSLTKKPVKAMLTGPVT
ILCWSFPREDISKEDICNQIAISLRDEVLDLENSGINIIQIDEPALREGLPLRTHEWNNYLRWAVKSFKICSSGVKNSTQ
IHTHMCYCEFNDIMPAIVDLDADVITIETSRSDMELLEFFKTFKYPNAIGPGVYDIHSPNIPSVQSIEKLLKKALKYISI
QQLWVNPDCGLKTRNWTETSLALQNMLQATLNIRKEYFKK
>Mature_759_residues
AIKNHILGFPRIGLNRELKFALEKYWSKKNTLEELLLIGKNIRKENWKNQIDSGMDYVTVGDFAWYDHVLNISMMINNIP
ERHNPNNHILNIDTLFKVARGSKGVDNNICSASEMTKWFNTNYHYIVPEFTSNQKFYFAWKQILEETDEALSLGYKVKPV
LLGPLTYLWLGKVKNSKINKLDLLKKILPIYIQVFKELSSRNINWIQIDEPILVLDIPKNWKKEFQSTYKFLDGKIKILL
ATYFGDITHNLDIINKLSIQGLHIDLVSSKYDLLKLSKSINNNFLLSLGIINGRNIWKTNLLEWFYKLKDFMKINNNFWI
SSSCSLLHVPLDITIEENLTDFVKSWFSFGIQKCLEISLLSQVLQNNLDIKELKNWIKPIHEYKSSNIVNNTSVQKRTLK
ISSEQFIRKNEFSVRSKIQKETLCLPVLPTTTIGSFPQTSEIRKLRLDYKNKKINQLDYEKQIKIHIKKNIIQQEQLGLD
VLVHGEPERNDMVEYFSEYLEGFVFTTYGWVQSYGSRCVKPPIIVGDISRITPMTVMWSKYAQSLTKKPVKAMLTGPVTI
LCWSFPREDISKEDICNQIAISLRDEVLDLENSGINIIQIDEPALREGLPLRTHEWNNYLRWAVKSFKICSSGVKNSTQI
HTHMCYCEFNDIMPAIVDLDADVITIETSRSDMELLEFFKTFKYPNAIGPGVYDIHSPNIPSVQSIEKLLKKALKYISIQ
QLWVNPDCGLKTRNWTETSLALQNMLQATLNIRKEYFKK

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=755, Percent_Identity=58.4105960264901, Blast_Score=965, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=773, Percent_Identity=43.4670116429496, Blast_Score=638, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_BUCCC (Q058E7)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802595.1
- ProteinModelPortal:   Q058E7
- SMR:   Q058E7
- STRING:   Q058E7
- EnsemblBacteria:   EBBUCT00000001541
- GeneID:   4440657
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_017
- eggNOG:   COG0620
- GeneTree:   EBGT00050000007816
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BioCyc:   BAPH372461:BCC_017-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 88415; Mature: 88284

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKNHILGFPRIGLNRELKFALEKYWSKKNTLEELLLIGKNIRKENWKNQIDSGMDYVT
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEE
VGDFAWYDHVLNISMMINNIPERHNPNNHILNIDTLFKVARGSKGVDNNICSASEMTKWF
ECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHH
NTNYHYIVPEFTSNQKFYFAWKQILEETDEALSLGYKVKPVLLGPLTYLWLGKVKNSKIN
CCCCEEEECEECCCCEEHHHHHHHHHHHHHHHHCCCEECEEEECHHHHHHHHCCCCCCCH
KLDLLKKILPIYIQVFKELSSRNINWIQIDEPILVLDIPKNWKKEFQSTYKFLDGKIKIL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHCCCCEEEE
LATYFGDITHNLDIINKLSIQGLHIDLVSSKYDLLKLSKSINNNFLLSLGIINGRNIWKT
EEEHHHHHHCCHHHHHHHEECEEEEEEECCCHHHHHHHHHCCCCEEEEEEEECCCCHHHH
NLLEWFYKLKDFMKINNNFWISSSCSLLHVPLDITIEENLTDFVKSWFSFGIQKCLEISL
HHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
LSQVLQNNLDIKELKNWIKPIHEYKSSNIVNNTSVQKRTLKISSEQFIRKNEFSVRSKIQ
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEECHHHHHHHHHHHHHHHHH
KETLCLPVLPTTTIGSFPQTSEIRKLRLDYKNKKINQLDYEKQIKIHIKKNIIQQEQLGL
HHCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCC
DVLVHGEPERNDMVEYFSEYLEGFVFTTYGWVQSYGSRCVKPPIIVGDISRITPMTVMWS
EEEEECCCCCHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCEEEECCHHHCHHHHHHH
KYAQSLTKKPVKAMLTGPVTILCWSFPREDISKEDICNQIAISLRDEVLDLENSGINIIQ
HHHHHHHHCCHHHHHCCCEEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IDEPALREGLPLRTHEWNNYLRWAVKSFKICSSGVKNSTQIHTHMCYCEFNDIMPAIVDL
ECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHHCC
DADVITIETSRSDMELLEFFKTFKYPNAIGPGVYDIHSPNIPSVQSIEKLLKKALKYISI
CCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
QQLWVNPDCGLKTRNWTETSLALQNMLQATLNIRKEYFKK
HHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AIKNHILGFPRIGLNRELKFALEKYWSKKNTLEELLLIGKNIRKENWKNQIDSGMDYVT
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEE
VGDFAWYDHVLNISMMINNIPERHNPNNHILNIDTLFKVARGSKGVDNNICSASEMTKWF
ECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHH
NTNYHYIVPEFTSNQKFYFAWKQILEETDEALSLGYKVKPVLLGPLTYLWLGKVKNSKIN
CCCCEEEECEECCCCEEHHHHHHHHHHHHHHHHCCCEECEEEECHHHHHHHHCCCCCCCH
KLDLLKKILPIYIQVFKELSSRNINWIQIDEPILVLDIPKNWKKEFQSTYKFLDGKIKIL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHCCCCEEEE
LATYFGDITHNLDIINKLSIQGLHIDLVSSKYDLLKLSKSINNNFLLSLGIINGRNIWKT
EEEHHHHHHCCHHHHHHHEECEEEEEEECCCHHHHHHHHHCCCCEEEEEEEECCCCHHHH
NLLEWFYKLKDFMKINNNFWISSSCSLLHVPLDITIEENLTDFVKSWFSFGIQKCLEISL
HHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
LSQVLQNNLDIKELKNWIKPIHEYKSSNIVNNTSVQKRTLKISSEQFIRKNEFSVRSKIQ
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHEEECHHHHHHHHHHHHHHHHH
KETLCLPVLPTTTIGSFPQTSEIRKLRLDYKNKKINQLDYEKQIKIHIKKNIIQQEQLGL
HHCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCC
DVLVHGEPERNDMVEYFSEYLEGFVFTTYGWVQSYGSRCVKPPIIVGDISRITPMTVMWS
EEEEECCCCCHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCEEEECCHHHCHHHHHHH
KYAQSLTKKPVKAMLTGPVTILCWSFPREDISKEDICNQIAISLRDEVLDLENSGINIIQ
HHHHHHHHCCHHHHHCCCEEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IDEPALREGLPLRTHEWNNYLRWAVKSFKICSSGVKNSTQIHTHMCYCEFNDIMPAIVDL
ECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCHHHHCC
DADVITIETSRSDMELLEFFKTFKYPNAIGPGVYDIHSPNIPSVQSIEKLLKKALKYISI
CCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
QQLWVNPDCGLKTRNWTETSLALQNMLQATLNIRKEYFKK
HHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA