Click here to switch to the map view.

The map label for this gene is thdF

Identifier: 116514958

GI number: 116514958

Start: 9061

End: 10425

Strand: Direct

Name: thdF

Synonym: BCc_008

Alternate gene names: 116514958

Gene position: 9061-10425 (Clockwise)

Preceding gene: 116514957

Following gene: 116514959

Centisome position: 2.18

GC content: 18.97

Gene sequence:

>1365_bases
ATGAAATTTTTTGATACTATAGTTTCTCCAGCTACTGTGATCGGTCGTTCAGGTATAGGAATTATAAGAATTTCTGGAAT
TTCAGTATTAAAAATAATAAAAAAATTTTTAAAAATTTCTATGAAAGAAAGATTCGCATATTTTTCATCTTTTTATGATG
TTAAAAATAATTTATTAGATCAAGGAATAGCTTTATTTTTTTTAGCTCCTAAATCTTTTACAGGAGAAAATATTTTAGAA
TTTCATAGTCATGGAAACCCTATTATATTAGATTTATTAATTAAAAATATTTTAACAATTAAAAATGTTAGGATAGCGAA
TCCAGGTGAATTTTCAAAAAGAGCATTTTTAAATAATAAAATTGATTTAGTTCAAGCTGAAGCTATTAATGATATAATAA
ATGCTGAATCACATTTATCAGTTAAAGCGGCTTTGTCTTCATTAAGGGGTACTTTTTCTAAAAAAATAAATAAAATTTTA
TTTAATTTAAAAGATATATATTCTGAAATTGAAGCTATTATTAATTTTCCTGAAGAATTAAACGATTTAAATATACAAAA
AAATATTAAAAAAAAATTATCTTTTATTATAAAAATGATAACTAATTTATTAGATGAAACACATAAAAATTATATATTTT
CTAATACTATCAAAATAGTTATTGCTGGACCTCCAAATGTAGGTAAATCTAGTTTATTAAATTTTCTATCAAAAGAAAAA
GTTTCTATAGTTACTAATATTCCAGGTACTACTCGTGATGTTATACATAAAAATATATGGTTTAATGGCGTATGTTGTGA
ATTTTTAGATACTGCGGGATTACAAAAAAGTCAAGATATTATTGAAGTAATAGGTATTAAATTAGCAAAAAAACATATTA
AATCTTGTAATCATATTTTTTTAATGTTTGATGTTACAAAAAAAAAAATGATTAATAATAATTTTATAAAAAATATTGTT
AATAACTTAAAAAAAAATCAAAATATTACTTTTATTTTTAACAAAATTGATTTAATTAATAAAAAACCATATATAAGTAT
TATTTATAAAAAATTTGAATGTATTTATTTATCTCTTAAAAAAAATATTGGTATAGAATATTTAAAAAATAAAATTTTAG
AAATTACTACATTACATAATAATGTAGAAAGCACATTTTTAGCAAAAAAAAGACATATATCAGCATTAAAAAAATCATTG
ATGTATTTAATTAATGGTAAAAGAAATTGGATGAAAAATTTATATTTAGAATTATTATCTGATGATATTCGTTTATCTAT
AAAATATTTATTAAAAATAACAGGAAAATTTAATAGTGAAGATTTATTAGATAAAATTTTTTCTAAATTTTGTATTGGAA
AATAA

Upstream 100 bases:

>100_bases
TACAGTTTTTCAAAAAAAATACTTAATTTTATTAAAATTTTTTTTTTATTTTATTAAATTATTTAAAAAAGAGTTTTATT
ATATATGGAATAAAATAGTT

Downstream 100 bases:

>100_bases
TTTTGGTATTAAAAAAAATATTTAAAAAATATATAGATTTTGCCCGGAGGCGGAATTGAACCACCGACACGGGGATTTTC
AGTCCCCTGCTCTACCGACT

Product: GTP-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKFFDTIVSPATVIGRSGIGIIRISGISVLKIIKKFLKISMKERFAYFSSFYDVKNNLLDQGIALFFLAPKSFTGENILE
FHSHGNPIILDLLIKNILTIKNVRIANPGEFSKRAFLNNKIDLVQAEAINDIINAESHLSVKAALSSLRGTFSKKINKIL
FNLKDIYSEIEAIINFPEELNDLNIQKNIKKKLSFIIKMITNLLDETHKNYIFSNTIKIVIAGPPNVGKSSLLNFLSKEK
VSIVTNIPGTTRDVIHKNIWFNGVCCEFLDTAGLQKSQDIIEVIGIKLAKKHIKSCNHIFLMFDVTKKKMINNNFIKNIV
NNLKKNQNITFIFNKIDLINKKPYISIIYKKFECIYLSLKKNIGIEYLKNKILEITTLHNNVESTFLAKKRHISALKKSL
MYLINGKRNWMKNLYLELLSDDIRLSIKYLLKITGKFNSEDLLDKIFSKFCIGK

Sequences:

>Translated_454_residues
MKFFDTIVSPATVIGRSGIGIIRISGISVLKIIKKFLKISMKERFAYFSSFYDVKNNLLDQGIALFFLAPKSFTGENILE
FHSHGNPIILDLLIKNILTIKNVRIANPGEFSKRAFLNNKIDLVQAEAINDIINAESHLSVKAALSSLRGTFSKKINKIL
FNLKDIYSEIEAIINFPEELNDLNIQKNIKKKLSFIIKMITNLLDETHKNYIFSNTIKIVIAGPPNVGKSSLLNFLSKEK
VSIVTNIPGTTRDVIHKNIWFNGVCCEFLDTAGLQKSQDIIEVIGIKLAKKHIKSCNHIFLMFDVTKKKMINNNFIKNIV
NNLKKNQNITFIFNKIDLINKKPYISIIYKKFECIYLSLKKNIGIEYLKNKILEITTLHNNVESTFLAKKRHISALKKSL
MYLINGKRNWMKNLYLELLSDDIRLSIKYLLKITGKFNSEDLLDKIFSKFCIGK
>Mature_454_residues
MKFFDTIVSPATVIGRSGIGIIRISGISVLKIIKKFLKISMKERFAYFSSFYDVKNNLLDQGIALFFLAPKSFTGENILE
FHSHGNPIILDLLIKNILTIKNVRIANPGEFSKRAFLNNKIDLVQAEAINDIINAESHLSVKAALSSLRGTFSKKINKIL
FNLKDIYSEIEAIINFPEELNDLNIQKNIKKKLSFIIKMITNLLDETHKNYIFSNTIKIVIAGPPNVGKSSLLNFLSKEK
VSIVTNIPGTTRDVIHKNIWFNGVCCEFLDTAGLQKSQDIIEVIGIKLAKKHIKSCNHIFLMFDVTKKKMINNNFIKNIV
NNLKKNQNITFIFNKIDLINKKPYISIIYKKFECIYLSLKKNIGIEYLKNKILEITTLHNNVESTFLAKKRHISALKKSL
MYLINGKRNWMKNLYLELLSDDIRLSIKYLLKITGKFNSEDLLDKIFSKFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=27.7777777777778, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI193082993, Length=461, Percent_Identity=27.5488069414317, Blast_Score=206, Evalue=5e-53,
Organism=Homo sapiens, GI193082988, Length=501, Percent_Identity=27.1457085828343, Blast_Score=203, Evalue=3e-52,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=41.019955654102, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17507259, Length=455, Percent_Identity=28.5714285714286, Blast_Score=185, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6323665, Length=492, Percent_Identity=33.9430894308943, Blast_Score=213, Evalue=7e-56,
Organism=Drosophila melanogaster, GI45550813, Length=472, Percent_Identity=28.3898305084746, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_BUCCC (Q058F5)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802587.1
- STRING:   Q058F5
- EnsemblBacteria:   EBBUCT00000001280
- GeneID:   4440648
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_008
- eggNOG:   COG0486
- GeneTree:   EBGT00050000007774
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- BioCyc:   BAPH372461:BCC_008-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51976; Mature: 51976

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 80-80 BINDING 120-120 BINDING 454-454

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFFDTIVSPATVIGRSGIGIIRISGISVLKIIKKFLKISMKERFAYFSSFYDVKNNLLD
CCHHHHHCCCHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGIALFFLAPKSFTGENILEFHSHGNPIILDLLIKNILTIKNVRIANPGEFSKRAFLNNK
CCEEEEEECCCCCCCCHHHHHHCCCCCEEHHHHHHHHHHHCCEEECCCCCHHHHHHHCCC
IDLVQAEAINDIINAESHLSVKAALSSLRGTFSKKINKILFNLKDIYSEIEAIINFPEEL
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
NDLNIQKNIKKKLSFIIKMITNLLDETHKNYIFSNTIKIVIAGPPNVGKSSLLNFLSKEK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECEEEEEEECCCCCCHHHHHHHHHHCC
VSIVTNIPGTTRDVIHKNIWFNGVCCEFLDTAGLQKSQDIIEVIGIKLAKKHIKSCNHIF
EEEEECCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
LMFDVTKKKMINNNFIKNIVNNLKKNQNITFIFNKIDLINKKPYISIIYKKFECIYLSLK
EEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHCCCCCHHHHHHHHHHHEEEEHH
KNIGIEYLKNKILEITTLHNNVESTFLAKKRHISALKKSLMYLINGKRNWMKNLYLELLS
HCCCHHHHHHHHEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DDIRLSIKYLLKITGKFNSEDLLDKIFSKFCIGK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKFFDTIVSPATVIGRSGIGIIRISGISVLKIIKKFLKISMKERFAYFSSFYDVKNNLLD
CCHHHHHCCCHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGIALFFLAPKSFTGENILEFHSHGNPIILDLLIKNILTIKNVRIANPGEFSKRAFLNNK
CCEEEEEECCCCCCCCHHHHHHCCCCCEEHHHHHHHHHHHCCEEECCCCCHHHHHHHCCC
IDLVQAEAINDIINAESHLSVKAALSSLRGTFSKKINKILFNLKDIYSEIEAIINFPEEL
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
NDLNIQKNIKKKLSFIIKMITNLLDETHKNYIFSNTIKIVIAGPPNVGKSSLLNFLSKEK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECEEEEEEECCCCCCHHHHHHHHHHCC
VSIVTNIPGTTRDVIHKNIWFNGVCCEFLDTAGLQKSQDIIEVIGIKLAKKHIKSCNHIF
EEEEECCCCCHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
LMFDVTKKKMINNNFIKNIVNNLKKNQNITFIFNKIDLINKKPYISIIYKKFECIYLSLK
EEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHCCCCCHHHHHHHHHHHEEEEHH
KNIGIEYLKNKILEITTLHNNVESTFLAKKRHISALKKSLMYLINGKRNWMKNLYLELLS
HCCCHHHHHHHHEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
DDIRLSIKYLLKITGKFNSEDLLDKIFSKFCIGK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA