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The map label for this gene is yjeA [H]

Identifier: 116332661

GI number: 116332661

Start: 276384

End: 277331

Strand: Reverse

Name: yjeA [H]

Synonym: LBJ_4242

Alternate gene names: 116332661

Gene position: 277331-276384 (Counterclockwise)

Preceding gene: 116332663

Following gene: 116332660

Centisome position: 92.52

GC content: 41.24

Gene sequence:

>948_bases
ATGAATGAATTGAGCGGGGAAGTTCTTATCAAACGGGCGCAATTCTTGTCCGTTCTACGGAAATTTTTTGAGAAAAAAAA
TTATTTGGAAATGGATACTCCCTGTCTGAAAGCCGTCCCTTCGATGGAACCGTATTTGGATCCTTTTTTAGTCCGATCTC
CCTCAAGAAAAGAAAAAGGTTATTTGATCACGTCTCCCGAATATTCTCTCAAAGAGATCCTCTCCAAAGGTTTGGAAAAA
ATTTATGAAATTACGCATACGTTTCGTTCCGGCGAGGAAGGAAGTCCGTTTCACAGCGCCGAATTTTTGATGCTCGAATT
TTATACCGTTGGAATCTCTCTCGAAGATCTGATGAATTTTTGTACGGAACTTTTGGAAGTTCTGGATTGTGAATTCCAGG
CTTTCGGTTTCGATCGAAATAAAGTTCGAAAAATATCCGTAGAAGAATCTCTTAGGAAATACGCTCATTGCGGAATTTCC
AAATCGGAACTCGACCGAGTGATTTTGGAACGCAGGTTAAGCGAAATTCCGGCCGAAAAAAGAGCTTATGAAGATTCGTT
TTTTATTGTATTCTTGAATCTTGTAGAGGCGCATCTACCGAAAGAGTTTGTGTTTTTATACGATTACCCTCCAGAATTGG
CGGCCCTTTCCAAAATCGAGTCCGGTTTTGCAAAACGTTTCGAACTCTATTATGGAAGTTTGGAATTGGGCAATGCATTC
GAAGAGTTAACCGATCCGGTCGAACAGATTTCCCGATTTTGTCACGAACAAGATCTGAGAAAGAATTTGGGTAAAGAGGT
ATTCTCTGTCGATCCCGGTCTCGAACGCGCGCTTCGAGAAGGAATTCCGGATTCCTGCGGAATTTCGATTGGTTTGGATC
GACTTTTACTTTGTATTCTAGGCGGAAGTTCTTTGAGGGAAATTAGTCCATATTACGGAAAGTTCTGA

Upstream 100 bases:

>100_bases
TTTCTTCGAATTTCGAAACGTTCGTATTTCTGAATTGGATTCACAAAGTTCATATCGTTTTCGTTTTTAAAAACAATCAA
GAGGAAAACAATAAAGTCTG

Downstream 100 bases:

>100_bases
GTTTCAGTGTCAATTTTAGAACGATTCGAACCGAATTTATAAAAGGAGATAAAACTTTGGTTGCATGGAACCAATTCTTC
TCTCAATCTTGATGCGGCGG

Product: tRNA synthetase class II (D, K and N)

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MNELSGEVLIKRAQFLSVLRKFFEKKNYLEMDTPCLKAVPSMEPYLDPFLVRSPSRKEKGYLITSPEYSLKEILSKGLEK
IYEITHTFRSGEEGSPFHSAEFLMLEFYTVGISLEDLMNFCTELLEVLDCEFQAFGFDRNKVRKISVEESLRKYAHCGIS
KSELDRVILERRLSEIPAEKRAYEDSFFIVFLNLVEAHLPKEFVFLYDYPPELAALSKIESGFAKRFELYYGSLELGNAF
EELTDPVEQISRFCHEQDLRKNLGKEVFSVDPGLERALREGIPDSCGISIGLDRLLLCILGGSSLREISPYYGKF

Sequences:

>Translated_315_residues
MNELSGEVLIKRAQFLSVLRKFFEKKNYLEMDTPCLKAVPSMEPYLDPFLVRSPSRKEKGYLITSPEYSLKEILSKGLEK
IYEITHTFRSGEEGSPFHSAEFLMLEFYTVGISLEDLMNFCTELLEVLDCEFQAFGFDRNKVRKISVEESLRKYAHCGIS
KSELDRVILERRLSEIPAEKRAYEDSFFIVFLNLVEAHLPKEFVFLYDYPPELAALSKIESGFAKRFELYYGSLELGNAF
EELTDPVEQISRFCHEQDLRKNLGKEVFSVDPGLERALREGIPDSCGISIGLDRLLLCILGGSSLREISPYYGKF
>Mature_315_residues
MNELSGEVLIKRAQFLSVLRKFFEKKNYLEMDTPCLKAVPSMEPYLDPFLVRSPSRKEKGYLITSPEYSLKEILSKGLEK
IYEITHTFRSGEEGSPFHSAEFLMLEFYTVGISLEDLMNFCTELLEVLDCEFQAFGFDRNKVRKISVEESLRKYAHCGIS
KSELDRVILERRLSEIPAEKRAYEDSFFIVFLNLVEAHLPKEFVFLYDYPPELAALSKIESGFAKRFELYYGSLELGNAF
EELTDPVEQISRFCHEQDLRKNLGKEVFSVDPGLERALREGIPDSCGISIGLDRLLLCILGGSSLREISPYYGKF

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=336, Percent_Identity=23.8095238095238, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI5031815, Length=336, Percent_Identity=23.8095238095238, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87082379, Length=314, Percent_Identity=32.8025477707006, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1790571, Length=323, Percent_Identity=27.5541795665635, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1789256, Length=323, Percent_Identity=26.625386996904, Blast_Score=118, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324256, Length=354, Percent_Identity=26.271186440678, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 36254; Mature: 36254

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELSGEVLIKRAQFLSVLRKFFEKKNYLEMDTPCLKAVPSMEPYLDPFLVRSPSRKEKG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHCHHHHCCCCCCCCC
YLITSPEYSLKEILSKGLEKIYEITHTFRSGEEGSPFHSAEFLMLEFYTVGISLEDLMNF
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
CTELLEVLDCEFQAFGFDRNKVRKISVEESLRKYAHCGISKSELDRVILERRLSEIPAEK
HHHHHHHHCCCHHHCCCCHHHHHEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHH
RAYEDSFFIVFLNLVEAHLPKEFVFLYDYPPELAALSKIESGFAKRFELYYGSLELGNAF
HHHCCHHHHHHHHHHHHHCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
EELTDPVEQISRFCHEQDLRKNLGKEVFSVDPGLERALREGIPDSCGISIGLDRLLLCIL
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
GGSSLREISPYYGKF
CCCHHHHCCHHCCCC
>Mature Secondary Structure
MNELSGEVLIKRAQFLSVLRKFFEKKNYLEMDTPCLKAVPSMEPYLDPFLVRSPSRKEKG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHCHHHHCCCCCCCCC
YLITSPEYSLKEILSKGLEKIYEITHTFRSGEEGSPFHSAEFLMLEFYTVGISLEDLMNF
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHH
CTELLEVLDCEFQAFGFDRNKVRKISVEESLRKYAHCGISKSELDRVILERRLSEIPAEK
HHHHHHHHCCCHHHCCCCHHHHHEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHH
RAYEDSFFIVFLNLVEAHLPKEFVFLYDYPPELAALSKIESGFAKRFELYYGSLELGNAF
HHHCCHHHHHHHHHHHHHCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
EELTDPVEQISRFCHEQDLRKNLGKEVFSVDPGLERALREGIPDSCGISIGLDRLLLCIL
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
GGSSLREISPYYGKF
CCCHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA