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The map label for this gene is speE [H]

Identifier: 116332648

GI number: 116332648

Start: 259578

End: 260420

Strand: Reverse

Name: speE [H]

Synonym: LBJ_4228

Alternate gene names: 116332648

Gene position: 260420-259578 (Counterclockwise)

Preceding gene: 116332649

Following gene: 116332647

Centisome position: 86.88

GC content: 45.55

Gene sequence:

>843_bases
ATGGAACTCTGGTTAGACGAAGCATTAGAACTCAAAAATGGGCGCGCTCTTAAAATCAAGGTAAAGGAATTCCTACATTC
CAGGACGACTCCTTTTCAAAAGATCGACGTATTCGAATCCGTAGGTTTCGGAAGAATGTTCACTCTCGACGGAGTGGTCA
TGATGACCGAAGCCGATGAGTTCGCGTATCATGAGATGATCGTTCACGTTCCTATGATGAGTCATCCGAACCCGGAAAAG
GTTCTGGTGATCGGAGGGGGAGACGGAGGAACGGTTCGCGAGGTTCTCAAACATCCTTCGGTAAAAGAAGTGCATCTTTG
TGAAATCGATAAGGGTGTGATCGATGTTTGTTACGAATACTTTCCCGAGATCGCAAATGCGATGAAGGATCCTCGAGTAA
AACACGCCTACGAAGACGGTGCGAAATACGTAAAAGATTATCGGAATTATTTCGATTGTATTATGGTTGATTCTTCCGAT
CCCGTAGGTCCTGCCGAAGTCCTTTTCAAAAGACCTTTCTATGAAACGATGGCCGACTGTTTGAAAGAAGGCGGAATTTG
CACGACCCAAGGAGAAAGCTACTATTATCACGGACCGATTATCCGTGAGTTGTTCAACTTCATTCCGGAGATTTTCAAAC
ACTGCGGTTACTATTACACTGTTGTTCCGACTTATCCTTCCGGAATCATCGGGTTTACATATTGCTCTAAGGGACCGGAT
CCTTATACGGTGATTCCCGATCTCCAAAGGGTTCCGCAAGGACTGAAGTATTATTCTGCGGAGATGCACAAGGCGGCCTT
CGTGCTTCCTCAGTTCGCGCAGAAACATATCGTTCGAAAATAA

Upstream 100 bases:

>100_bases
TAAATGTTATGACCCGAAAGTGGCTTCCGAGTTTACCCGTAAGTTTTTCCAAGGCGGCGCGATGAGACTCACCGTGACCA
ACCGCTTCTAAGGAAAATTG

Downstream 100 bases:

>100_bases
ATTCTTATATCCGGGGGTGGAACGATGAATTTTCAAAGTAAGTTCCTAACCCGGAGCCAATCTCTCAAATCCCTTCTTTG
TGTCGGACTCGATCCAGACT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 2; PAPT 2; SPDSY 2 [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MELWLDEALELKNGRALKIKVKEFLHSRTTPFQKIDVFESVGFGRMFTLDGVVMMTEADEFAYHEMIVHVPMMSHPNPEK
VLVIGGGDGGTVREVLKHPSVKEVHLCEIDKGVIDVCYEYFPEIANAMKDPRVKHAYEDGAKYVKDYRNYFDCIMVDSSD
PVGPAEVLFKRPFYETMADCLKEGGICTTQGESYYYHGPIIRELFNFIPEIFKHCGYYYTVVPTYPSGIIGFTYCSKGPD
PYTVIPDLQRVPQGLKYYSAEMHKAAFVLPQFAQKHIVRK

Sequences:

>Translated_280_residues
MELWLDEALELKNGRALKIKVKEFLHSRTTPFQKIDVFESVGFGRMFTLDGVVMMTEADEFAYHEMIVHVPMMSHPNPEK
VLVIGGGDGGTVREVLKHPSVKEVHLCEIDKGVIDVCYEYFPEIANAMKDPRVKHAYEDGAKYVKDYRNYFDCIMVDSSD
PVGPAEVLFKRPFYETMADCLKEGGICTTQGESYYYHGPIIRELFNFIPEIFKHCGYYYTVVPTYPSGIIGFTYCSKGPD
PYTVIPDLQRVPQGLKYYSAEMHKAAFVLPQFAQKHIVRK
>Mature_280_residues
MELWLDEALELKNGRALKIKVKEFLHSRTTPFQKIDVFESVGFGRMFTLDGVVMMTEADEFAYHEMIVHVPMMSHPNPEK
VLVIGGGDGGTVREVLKHPSVKEVHLCEIDKGVIDVCYEYFPEIANAMKDPRVKHAYEDGAKYVKDYRNYFDCIMVDSSD
PVGPAEVLFKRPFYETMADCLKEGGICTTQGESYYYHGPIIRELFNFIPEIFKHCGYYYTVVPTYPSGIIGFTYCSKGPD
PYTVIPDLQRVPQGLKYYSAEMHKAAFVLPQFAQKHIVRK

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=281, Percent_Identity=45.1957295373665, Blast_Score=259, Evalue=2e-69,
Organism=Escherichia coli, GI1786312, Length=266, Percent_Identity=37.593984962406, Blast_Score=191, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI25154106, Length=269, Percent_Identity=44.6096654275093, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI212645616, Length=321, Percent_Identity=37.3831775700935, Blast_Score=208, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6325326, Length=269, Percent_Identity=44.9814126394052, Blast_Score=256, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6323175, Length=274, Percent_Identity=39.4160583941606, Blast_Score=206, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24645443, Length=277, Percent_Identity=41.8772563176895, Blast_Score=231, Evalue=5e-61,
Organism=Drosophila melanogaster, GI21355941, Length=214, Percent_Identity=42.9906542056075, Blast_Score=181, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32042; Mature: 32042

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELWLDEALELKNGRALKIKVKEFLHSRTTPFQKIDVFESVGFGRMFTLDGVVMMTEADE
CCCCHHHHHHHCCCCEEEEEHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCEEEEECCHH
FAYHEMIVHVPMMSHPNPEKVLVIGGGDGGTVREVLKHPSVKEVHLCEIDKGVIDVCYEY
HHHHHHHHEECCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
FPEIANAMKDPRVKHAYEDGAKYVKDYRNYFDCIMVDSSDPVGPAEVLFKRPFYETMADC
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHCCHHHHHHHH
LKEGGICTTQGESYYYHGPIIRELFNFIPEIFKHCGYYYTVVPTYPSGIIGFTYCSKGPD
HHHCCEEEECCCCEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCC
PYTVIPDLQRVPQGLKYYSAEMHKAAFVLPQFAQKHIVRK
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MELWLDEALELKNGRALKIKVKEFLHSRTTPFQKIDVFESVGFGRMFTLDGVVMMTEADE
CCCCHHHHHHHCCCCEEEEEHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCEEEEECCHH
FAYHEMIVHVPMMSHPNPEKVLVIGGGDGGTVREVLKHPSVKEVHLCEIDKGVIDVCYEY
HHHHHHHHEECCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
FPEIANAMKDPRVKHAYEDGAKYVKDYRNYFDCIMVDSSDPVGPAEVLFKRPFYETMADC
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHCCHHHHHHHH
LKEGGICTTQGESYYYHGPIIRELFNFIPEIFKHCGYYYTVVPTYPSGIIGFTYCSKGPD
HHHCCEEEECCCCEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCC
PYTVIPDLQRVPQGLKYYSAEMHKAAFVLPQFAQKHIVRK
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12712204 [H]