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The map label for this gene is gra-orf5 [H]

Identifier: 116332637

GI number: 116332637

Start: 250463

End: 251161

Strand: Reverse

Name: gra-orf5 [H]

Synonym: LBJ_4217

Alternate gene names: 116332637

Gene position: 251161-250463 (Counterclockwise)

Preceding gene: 116332638

Following gene: 116332632

Centisome position: 83.79

GC content: 41.77

Gene sequence:

>699_bases
TTGTCTTCAGAGAAAGAAAACGATCTTATACTCGTTGCCGGAGCCGGTTCAGGAATTGGAAAGTCCGTATTAGAAAATCT
GAATCTATTGGATCAATCCGCGATCGGAGTTTCGAGGACGGGAGAAGTTTGGAAATCAAACGATGACTTCGAGTCTGGAA
AAAACTTTCAGTGTAATCTTTCCAGACAATCGGAGGTGCTTGAATTCGTTACGGCCTTAAAAAAAAGGGCTTTTTCCTTA
ACAGGAGTTTATTTTACTTTTGGAAGCGGCTTATTCAAGCCCGTAGGGCAAATCGCATTAGAAGAATGGAATGCACATTT
TGTTCTCAATCTGAATTCCCTTTTTTTGCTTACAAAAGAAATACTTCCTCTTCTAAAACCGGGTTCATTTTTGTGTTATC
TTTCTTCGACTGCGGGATACCAGGGTTTTTCGAATTCTACGGCTTATTGTGCGAGTCGACATGCTATTGCAGGTTTTGCA
AAAGCTCTACGGGAAGAGCTGAAGTCGGAAGGAATTCGAGTTATCACCGTTTATCCAGGAGCGGTATATACGGAAATTTG
GAGAGGTAGAGAAGGATTCGAGCAGGAGGATATGATTCCGGTTGACGATTTCGGGAAATGGCTCGCAACACTTTCTATCT
TACCGGATACCGTTTATCCGGACGAAATACATTTACTTCCTCGTAAAGGAATCTTGTAA

Upstream 100 bases:

>100_bases
CCCTACGGAAAATAATACTTCGGAGTCTGATCGTCAGAAAGTGTATGTTGCGGAATTCCCGGTTTATCCGGCTACATTTT
TGTAATAGAGAAAATTGATT

Downstream 100 bases:

>100_bases
GACTCGGTTGTGGATATCTTTTCGTGTAATATTATAAATATGAAAAAGGTTGATGGGGAATTTTTCAAGAAAGTGCAGTG
ACAAGGTCAAGATGTGAGCC

Product: Short chain dehydrogenase

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: ORF5 [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MSSEKENDLILVAGAGSGIGKSVLENLNLLDQSAIGVSRTGEVWKSNDDFESGKNFQCNLSRQSEVLEFVTALKKRAFSL
TGVYFTFGSGLFKPVGQIALEEWNAHFVLNLNSLFLLTKEILPLLKPGSFLCYLSSTAGYQGFSNSTAYCASRHAIAGFA
KALREELKSEGIRVITVYPGAVYTEIWRGREGFEQEDMIPVDDFGKWLATLSILPDTVYPDEIHLLPRKGIL

Sequences:

>Translated_232_residues
MSSEKENDLILVAGAGSGIGKSVLENLNLLDQSAIGVSRTGEVWKSNDDFESGKNFQCNLSRQSEVLEFVTALKKRAFSL
TGVYFTFGSGLFKPVGQIALEEWNAHFVLNLNSLFLLTKEILPLLKPGSFLCYLSSTAGYQGFSNSTAYCASRHAIAGFA
KALREELKSEGIRVITVYPGAVYTEIWRGREGFEQEDMIPVDDFGKWLATLSILPDTVYPDEIHLLPRKGIL
>Mature_231_residues
SSEKENDLILVAGAGSGIGKSVLENLNLLDQSAIGVSRTGEVWKSNDDFESGKNFQCNLSRQSEVLEFVTALKKRAFSLT
GVYFTFGSGLFKPVGQIALEEWNAHFVLNLNSLFLLTKEILPLLKPGSFLCYLSSTAGYQGFSNSTAYCASRHAIAGFAK
ALREELKSEGIRVITVYPGAVYTEIWRGREGFEQEDMIPVDDFGKWLATLSILPDTVYPDEIHLLPRKGIL

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Escherichia coli, GI1787335, Length=187, Percent_Identity=25.1336898395722, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1789378, Length=112, Percent_Identity=31.25, Blast_Score=62, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=199, Percent_Identity=30.6532663316583, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322227, Length=241, Percent_Identity=26.1410788381743, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24643142, Length=188, Percent_Identity=30.8510638297872, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI28571526, Length=203, Percent_Identity=29.064039408867, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355319, Length=89, Percent_Identity=39.3258426966292, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 25597; Mature: 25466

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEKENDLILVAGAGSGIGKSVLENLNLLDQSAIGVSRTGEVWKSNDDFESGKNFQCNL
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEC
SRQSEVLEFVTALKKRAFSLTGVYFTFGSGLFKPVGQIALEEWNAHFVLNLNSLFLLTKE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHH
ILPLLKPGSFLCYLSSTAGYQGFSNSTAYCASRHAIAGFAKALREELKSEGIRVITVYPG
HHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
AVYTEIWRGREGFEQEDMIPVDDFGKWLATLSILPDTVYPDEIHLLPRKGIL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
SSEKENDLILVAGAGSGIGKSVLENLNLLDQSAIGVSRTGEVWKSNDDFESGKNFQCNL
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEC
SRQSEVLEFVTALKKRAFSLTGVYFTFGSGLFKPVGQIALEEWNAHFVLNLNSLFLLTKE
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHH
ILPLLKPGSFLCYLSSTAGYQGFSNSTAYCASRHAIAGFAKALREELKSEGIRVITVYPG
HHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
AVYTEIWRGREGFEQEDMIPVDDFGKWLATLSILPDTVYPDEIHLLPRKGIL
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2583128; 7476861; 9831526 [H]