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The map label for this gene is cobJ [H]

Identifier: 116332612

GI number: 116332612

Start: 214213

End: 215643

Strand: Direct

Name: cobJ [H]

Synonym: LBJ_4185

Alternate gene names: 116332612

Gene position: 214213-215643 (Clockwise)

Preceding gene: 116332611

Following gene: 116332613

Centisome position: 71.46

GC content: 41.16

Gene sequence:

>1431_bases
ATGCAAATAGGAAGGTTGAACATCGTCGGTATCGGCCCGGGCAACGACATTCACATAACTCCGGCAGTTTTACAGGCGAT
TCAGGAAGCCGATCTTGTGATTGGTTACGCCACTTATATCGGTCTCGTTAAACACCATTTAGTCGGTAAACAAGTGACCC
AAACGGGTATGACGGAAGAAGTGAGTAGGGCGCAAACAGCTATTGATACGGCAAAAGAAGGAAAAATCGTTACCTTAATC
TCTTCGGGAGACGCAGGTGTCTATGGAATGGCAGGTCTTGTTTTTGAAATACTGCAAAAAACCGGCTGGAAAAAGGGAGA
TTCTCCCGAAATCAAAATGATTCCCGGAATTACCGCAGATAGTTCTTGCGCTTCCTTGATTGGCGCTCCTCTTGTTCACG
ATACAGCAAGAATTTCCCTCTCCGATCTTTTGACTCCTTGGTCCGTCATCGAAAATCGATTAGAATGCGCGGCTAAAGGG
GACTTCGTTATTACCCTTTATAATCCCGCTTCGGGACGAAGACAAAGACAGATCGTAGAAGCCGTGAACATTATTAAACG
ACATCGTTCGGGAACAACTCCGGTCGCTTTGGTCAAAAGCGCTTATAGAAAACAACAAAACGTCCAACTTTCGGATCTGG
ACAATTTTTTAGATTACGAAATTGGAATGAACACGACCGTAATCGTAGGCTCCTCAAATACTTTCGTTTATGAAGATTTT
ATGATCACTCCGAGAGGTTATACAAACAAATATTCTCTGAAAGATTCAAGTCTCAAAAAAGGACAGAAAAAAGGGTTTTC
GCTTCGTTCTACGGGGGATTTACAGAGCAAGGTAAATTCAGATAACGGGGACCGCCATCTGAGCTTGAACATAACGAAAA
TTCACGGGGCCTTCGTTAAAAGATCCACCACGATCATGGATTCGTCCGCGGCAAAAGCCGTAGAGTCCTTAAATTTACTT
TCACAAATTCAAAAACAGAATGCTAAAAAGGATTCTAAGTCTGTAGATTTCCAAAAAAACACGTTATCCTACATAGGTAG
GTTGGGTGGGGGAATTCTTTTTAAGAGTTTAGAAAAATTTTATATCGTCGGAAAAATCAAAGTTCCGATTCGATTTGAAG
ACTATGGCTTTGCAGACCCGGAGGAATATGGGGATCGGTACTTCGAATTAAAAATTATAGAGATTAAAAAAGTTCGAAAT
CTTCAATTCGAATTCCTGATGTTCATTCCAAACGAAATTTCTCCCCAAGAAATCTACGAGAAACTCGTTATCTACAGAAA
CTCCTCCGTAAGTGAACGTCTTATTACTTACGTAAAAAATCAAAGTGAAAAAGTTTTTTTAGACGGACAAGAATATACGG
ACATTTCCTGGCTGGGAACCATTCCCTCCGAAGTTTGGAATTTAGTCCGAGGGGTAATTCTAAAATGTTAG

Upstream 100 bases:

>100_bases
AAAATCTCACGATTGCAGTTTCCCGAATTCCATTTCCGAAACGACCTCTCGATCCTATAACACGTTCTGATTTTTCCATA
AACTCAACGGAGGAGAAAGA

Downstream 100 bases:

>100_bases
AGATTCAGGGTTTATGAAAGTTTATATCATCGGGGCCGGTCCGGGAGATCCGGAATTGATTACGGTAAAAGGAGCAAAAC
TCGTGGAAACCTGCCCGGTC

Product: precorrin-3B C(17)-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MQIGRLNIVGIGPGNDIHITPAVLQAIQEADLVIGYATYIGLVKHHLVGKQVTQTGMTEEVSRAQTAIDTAKEGKIVTLI
SSGDAGVYGMAGLVFEILQKTGWKKGDSPEIKMIPGITADSSCASLIGAPLVHDTARISLSDLLTPWSVIENRLECAAKG
DFVITLYNPASGRRQRQIVEAVNIIKRHRSGTTPVALVKSAYRKQQNVQLSDLDNFLDYEIGMNTTVIVGSSNTFVYEDF
MITPRGYTNKYSLKDSSLKKGQKKGFSLRSTGDLQSKVNSDNGDRHLSLNITKIHGAFVKRSTTIMDSSAAKAVESLNLL
SQIQKQNAKKDSKSVDFQKNTLSYIGRLGGGILFKSLEKFYIVGKIKVPIRFEDYGFADPEEYGDRYFELKIIEIKKVRN
LQFEFLMFIPNEISPQEIYEKLVIYRNSSVSERLITYVKNQSEKVFLDGQEYTDISWLGTIPSEVWNLVRGVILKC

Sequences:

>Translated_476_residues
MQIGRLNIVGIGPGNDIHITPAVLQAIQEADLVIGYATYIGLVKHHLVGKQVTQTGMTEEVSRAQTAIDTAKEGKIVTLI
SSGDAGVYGMAGLVFEILQKTGWKKGDSPEIKMIPGITADSSCASLIGAPLVHDTARISLSDLLTPWSVIENRLECAAKG
DFVITLYNPASGRRQRQIVEAVNIIKRHRSGTTPVALVKSAYRKQQNVQLSDLDNFLDYEIGMNTTVIVGSSNTFVYEDF
MITPRGYTNKYSLKDSSLKKGQKKGFSLRSTGDLQSKVNSDNGDRHLSLNITKIHGAFVKRSTTIMDSSAAKAVESLNLL
SQIQKQNAKKDSKSVDFQKNTLSYIGRLGGGILFKSLEKFYIVGKIKVPIRFEDYGFADPEEYGDRYFELKIIEIKKVRN
LQFEFLMFIPNEISPQEIYEKLVIYRNSSVSERLITYVKNQSEKVFLDGQEYTDISWLGTIPSEVWNLVRGVILKC
>Mature_476_residues
MQIGRLNIVGIGPGNDIHITPAVLQAIQEADLVIGYATYIGLVKHHLVGKQVTQTGMTEEVSRAQTAIDTAKEGKIVTLI
SSGDAGVYGMAGLVFEILQKTGWKKGDSPEIKMIPGITADSSCASLIGAPLVHDTARISLSDLLTPWSVIENRLECAAKG
DFVITLYNPASGRRQRQIVEAVNIIKRHRSGTTPVALVKSAYRKQQNVQLSDLDNFLDYEIGMNTTVIVGSSNTFVYEDF
MITPRGYTNKYSLKDSSLKKGQKKGFSLRSTGDLQSKVNSDNGDRHLSLNITKIHGAFVKRSTTIMDSSAAKAVESLNLL
SQIQKQNAKKDSKSVDFQKNTLSYIGRLGGGILFKSLEKFYIVGKIKVPIRFEDYGFADPEEYGDRYFELKIIEIKKVRN
LQFEFLMFIPNEISPQEIYEKLVIYRNSSVSERLITYVKNQSEKVFLDGQEYTDISWLGTIPSEVWNLVRGVILKC

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 52941; Mature: 52941

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGRLNIVGIGPGNDIHITPAVLQAIQEADLVIGYATYIGLVKHHLVGKQVTQTGMTEE
CCCCEEEEEEECCCCCEEECHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHCCCHHH
VSRAQTAIDTAKEGKIVTLISSGDAGVYGMAGLVFEILQKTGWKKGDSPEIKMIPGITAD
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
SSCASLIGAPLVHDTARISLSDLLTPWSVIENRLECAAKGDFVITLYNPASGRRQRQIVE
CHHHHHHCCCHHCCHHHEEHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHH
AVNIIKRHRSGTTPVALVKSAYRKQQNVQLSDLDNFLDYEIGMNTTVIVGSSNTFVYEDF
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEEE
MITPRGYTNKYSLKDSSLKKGQKKGFSLRSTGDLQSKVNSDNGDRHLSLNITKIHGAFVK
EECCCCCCCEECCCCHHHHHHHHCCCCCCCCCCHHHHCCCCCCCEEEEEEEEEECHHHHH
RSTTIMDSSAAKAVESLNLLSQIQKQNAKKDSKSVDFQKNTLSYIGRLGGGILFKSLEKF
CCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCE
YIVGKIKVPIRFEDYGFADPEEYGDRYFELKIIEIKKVRNLQFEFLMFIPNEISPQEIYE
EEEEEEEEEEEECCCCCCCHHHHCCEEEEEEEEEEEHHCCCCEEEEEECCCCCCHHHHHH
KLVIYRNSSVSERLITYVKNQSEKVFLDGQEYTDISWLGTIPSEVWNLVRGVILKC
HHHEECCCCHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQIGRLNIVGIGPGNDIHITPAVLQAIQEADLVIGYATYIGLVKHHLVGKQVTQTGMTEE
CCCCEEEEEEECCCCCEEECHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHCCCHHH
VSRAQTAIDTAKEGKIVTLISSGDAGVYGMAGLVFEILQKTGWKKGDSPEIKMIPGITAD
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
SSCASLIGAPLVHDTARISLSDLLTPWSVIENRLECAAKGDFVITLYNPASGRRQRQIVE
CHHHHHHCCCHHCCHHHEEHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHH
AVNIIKRHRSGTTPVALVKSAYRKQQNVQLSDLDNFLDYEIGMNTTVIVGSSNTFVYEDF
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEEE
MITPRGYTNKYSLKDSSLKKGQKKGFSLRSTGDLQSKVNSDNGDRHLSLNITKIHGAFVK
EECCCCCCCEECCCCHHHHHHHHCCCCCCCCCCHHHHCCCCCCCEEEEEEEEEECHHHHH
RSTTIMDSSAAKAVESLNLLSQIQKQNAKKDSKSVDFQKNTLSYIGRLGGGILFKSLEKF
CCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCE
YIVGKIKVPIRFEDYGFADPEEYGDRYFELKIIEIKKVRNLQFEFLMFIPNEISPQEIYE
EEEEEEEEEEEECCCCCCCHHHHCCEEEEEEEEEEEHHCCCCEEEEEECCCCCCHHHHHH
KLVIYRNSSVSERLITYVKNQSEKVFLDGQEYTDISWLGTIPSEVWNLVRGVILKC
HHHEECCCCHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]