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The map label for this gene is cobM [H]

Identifier: 116332613

GI number: 116332613

Start: 215657

End: 216421

Strand: Direct

Name: cobM [H]

Synonym: LBJ_4186

Alternate gene names: 116332613

Gene position: 215657-216421 (Clockwise)

Preceding gene: 116332612

Following gene: 116332614

Centisome position: 71.94

GC content: 42.88

Gene sequence:

>765_bases
ATGAAAGTTTATATCATCGGGGCCGGTCCGGGAGATCCGGAATTGATTACGGTAAAAGGAGCAAAACTCGTGGAAACCTG
CCCGGTCGTCCTTTATACCGGCTCACTTGTACCAAAGGTAGTGATCGAAAGAGCGAAGAAAGACGCAATTGTATTAGATT
CATCGAATATGATTCTGGATGACATCATTTCTGTAATTCTTAAAGCAAAAGAAAACGATCAGGACGTAGCCAGAGTTCAT
ACGGGAGATCCTTTTATTTTCGGTTCCATTGCGGAACAAATAAGAAAATTAGATTCTTTGAATATAGAATATGAAATTAT
TCCAGGGGTCAGCTCTTTTACCGCCGCGGCCGCGGCCTTAGGCAAAGAACTAACCCTTCCCGAAGTTTCTCAAACCGTCA
TCATTACACGTATGGAAGGAAGAACGCCCATGCCGGAAAAAGAAAGACTGGAAATTCTCGCTCAGTCCGGGGCAACCCTC
ACTCTTTTTTTAAGCGCCTTACACATTCGAAAGGTCGTAGAACGTTTAACTCCATACTACGGAGAGAAATGCCCGGTAGC
TGTAGTTCAGAAAGCAACCTGGCCGGAACAGAAAATTCTCATAGGAACATTAAACGACATTGTACAAAAAGTGAAGACGG
CAAAAATTTTCTCCACTGCGATTATTTTTGTCGGCCCAGTTTTAAACTGCCGCGACTTTGCCGACTCCAAACTTTACTCG
GCCGATTTTTCGCACAAATTCAGAAAAGTAAAAAAGAATTTATGA

Upstream 100 bases:

>100_bases
GGACAAGAATATACGGACATTTCCTGGCTGGGAACCATTCCCTCCGAAGTTTGGAATTTAGTCCGAGGGGTAATTCTAAA
ATGTTAGAGATTCAGGGTTT

Downstream 100 bases:

>100_bases
ACTCAGACTCAAAAAGAACGAAAGGACTCGTGATCGTTCATACCGGAAACGGTAAAGGAAAAACCACTGCCGCACTTGGA
ATCTTATTTCGCGCGTTAGG

Product: precorrin-3B C(17)-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKVYIIGAGPGDPELITVKGAKLVETCPVVLYTGSLVPKVVIERAKKDAIVLDSSNMILDDIISVILKAKENDQDVARVH
TGDPFIFGSIAEQIRKLDSLNIEYEIIPGVSSFTAAAAALGKELTLPEVSQTVIITRMEGRTPMPEKERLEILAQSGATL
TLFLSALHIRKVVERLTPYYGEKCPVAVVQKATWPEQKILIGTLNDIVQKVKTAKIFSTAIIFVGPVLNCRDFADSKLYS
ADFSHKFRKVKKNL

Sequences:

>Translated_254_residues
MKVYIIGAGPGDPELITVKGAKLVETCPVVLYTGSLVPKVVIERAKKDAIVLDSSNMILDDIISVILKAKENDQDVARVH
TGDPFIFGSIAEQIRKLDSLNIEYEIIPGVSSFTAAAAALGKELTLPEVSQTVIITRMEGRTPMPEKERLEILAQSGATL
TLFLSALHIRKVVERLTPYYGEKCPVAVVQKATWPEQKILIGTLNDIVQKVKTAKIFSTAIIFVGPVLNCRDFADSKLYS
ADFSHKFRKVKKNL
>Mature_254_residues
MKVYIIGAGPGDPELITVKGAKLVETCPVVLYTGSLVPKVVIERAKKDAIVLDSSNMILDDIISVILKAKENDQDVARVH
TGDPFIFGSIAEQIRKLDSLNIEYEIIPGVSSFTAAAAALGKELTLPEVSQTVIITRMEGRTPMPEKERLEILAQSGATL
TLFLSALHIRKVVERLTPYYGEKCPVAVVQKATWPEQKILIGTLNDIVQKVKTAKIFSTAIIFVGPVLNCRDFADSKLYS
ADFSHKFRKVKKNL

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=241, Percent_Identity=28.2157676348548, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 27908; Mature: 27908

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYIIGAGPGDPELITVKGAKLVETCPVVLYTGSLVPKVVIERAKKDAIVLDSSNMILD
CEEEEEECCCCCCCEEEECCCHHHHHCCEEEEECCHHHHHHHHHCCCCEEEEECCCCHHH
DIISVILKAKENDQDVARVHTGDPFIFGSIAEQIRKLDSLNIEYEIIPGVSSFTAAAAAL
HHHHHHHHCCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
GKELTLPEVSQTVIITRMEGRTPMPEKERLEILAQSGATLTLFLSALHIRKVVERLTPYY
CCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHC
GEKCPVAVVQKATWPEQKILIGTLNDIVQKVKTAKIFSTAIIFVGPVLNCRDFADSKLYS
CCCCCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHH
ADFSHKFRKVKKNL
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKVYIIGAGPGDPELITVKGAKLVETCPVVLYTGSLVPKVVIERAKKDAIVLDSSNMILD
CEEEEEECCCCCCCEEEECCCHHHHHCCEEEEECCHHHHHHHHHCCCCEEEEECCCCHHH
DIISVILKAKENDQDVARVHTGDPFIFGSIAEQIRKLDSLNIEYEIIPGVSSFTAAAAAL
HHHHHHHHCCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
GKELTLPEVSQTVIITRMEGRTPMPEKERLEILAQSGATLTLFLSALHIRKVVERLTPYY
CCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHC
GEKCPVAVVQKATWPEQKILIGTLNDIVQKVKTAKIFSTAIIFVGPVLNCRDFADSKLYS
CCCCCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHH
ADFSHKFRKVKKNL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]