Click here to switch to the map view.

The map label for this gene is yfgF [H]

Identifier: 116332511

GI number: 116332511

Start: 77013

End: 78731

Strand: Direct

Name: yfgF [H]

Synonym: LBJ_4069

Alternate gene names: 116332511

Gene position: 77013-78731 (Clockwise)

Preceding gene: 116332510

Following gene: 116332512

Centisome position: 25.69

GC content: 37.11

Gene sequence:

>1719_bases
ATGGGAAATAACGAATTCATTCAAAATACAAATTTGAATATCAAGCCGGTTATATTGATAATAGACGATGAAGTTGTTGT
ATTGAAGAGTATCAAGTGTGCAATTCAACTCAGTTTTGGAAGTCGGTTCGAAATAGAAATTGCGGAAGATGCAGATTCTG
CCATGGAAATTTTGGAGAGATGTAAATTCGAACAAATCGACGTTCCTTTAGTCATTTGTGATCAGGTACTACGTGAAAAA
CGCGGAGATGAACTACTAATTCAAATTCATCAGGAATACCCCAAAATTTATAAAGTAATGCTTACAGGTTATGCTTCCGC
AAAAACTCTTGGAAATGCTCTGAATAAAGCAAATCTTTATCGATATCTTTCAAAACCCTGGGATTCCGAGGATTTGATTC
TGACTATTTCCGAAGCGGTAAAAGCTTACTTTCAAAATCAAAAGGTGGCCGAACTTAGCTTTAAATTGGAAGAAACGTAT
CTGTTCAATCGTGAAACCTTGTTTCCGAATTTTGAAAATTTAAAACGAAGAATCGATCAAAGACTGATTGAAAACGAAAA
TTCCAGTCTTGCTCTCGTTCGAATAGAGTCTTTTAGTTCCATCGCTGAAAATTTCGGAATCGAAACATATCATAAGGTGT
TATCCGAATTCTTATCCGTCCTTAATTGTTTTGCAAGAGGTTACAACGGAAAAATTTTTCATATTTACGACAACATGATT
GCGATTCTGGCGAAGATCGAAGAGGATCGATTTTATTCTTTACTTAGCGCCGTTCATATTTTTCTCAGATCCGAATGCAT
TGAAGTGGATGGGATTTCTTTTCAGGTTAAAATTTCGATCGGAGTCTCATCGGACCAATCGGACGTATATGATAAGGCAA
GACTTGCAATGATGACTGCGAGCAATGATTCTTCCGTTGAATATGTTTCTTATTCGGAAGCGACCAACAAGGTGGATCGA
ATTTATGCAAATCTTAAGTTGGGTAGGAAGTTCAATGAGGCTTTGAATGCGGGAAATGTTATTCCTTACTTCCAGGGAAT
TTACGATAACAAGCTCAAAAGAATTACGAAGTACGAATGTTTAGCGAGAATTATGGAAGGAGGGCAGATTTATAATCCCG
CGATGTTTATTCCAATCGCAAAATCCACGGGATTGATTCGATTGCTCACTCCTTTAATGATTGAAAAGACGTTTAAATAT
TTTTCGAAACATTCCGAATATTCTTTTTCCATCAATATTTCCGAATCCGATTTGGATAAGAAAGGGTTTCCACTTTGGGT
GATGAATCGCCTTTTGCATTACAGCATATCTCCGGATCGGGTGATTTTTGAAATTCTAGAAAACGATCGTTGGAACGGAT
CTTCCAATTCTACTCGTTCTCTTCAAGAATTGAAAGAAATCGGTTGCAAAATTGCGATTGATGATTTCGGAGTGGAACGT
TCCAACTTTGAAAGACTGATGGAAATTCAACCGGATTTTATCAAAATAGACGGCAAATTTATACAGGGAATTCATGAAAA
TCAAACTTCATATCGATTGGCAGCCGCGATAACGGAAATGGCTCATACAATTGGTGCTAAAGTAGTTGCCAAGTTCGTAT
CCAAAGAAGAGGAGTTGGAAGCGGTGAAATCTTTGGATATAGACTATTCACAAGGGTATTATTTAATGGAGCCTGCGGAA
GAAATCGTTTATGCGGAAACGGTTTTAAACTTAGTTTGA

Upstream 100 bases:

>100_bases
ATTGATTCTTCTTAAGGGAAGTATCTGTTTTGAAGTTAGCATTGTTGGGTCTTTTTTTAAGAACTAGAGAGAATGAAAAT
AAAAATAAAGATGAAGTACG

Downstream 100 bases:

>100_bases
GTTTCTAATTGATTTTCACGAAATAGTTTCTCGGTGTTTCCTCATATCTGGGTAAAATTAAAATTCAGTGAATTTGCAAT
TTTAATTTTACCCCCTCATA

Product: two component complex response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MGNNEFIQNTNLNIKPVILIIDDEVVVLKSIKCAIQLSFGSRFEIEIAEDADSAMEILERCKFEQIDVPLVICDQVLREK
RGDELLIQIHQEYPKIYKVMLTGYASAKTLGNALNKANLYRYLSKPWDSEDLILTISEAVKAYFQNQKVAELSFKLEETY
LFNRETLFPNFENLKRRIDQRLIENENSSLALVRIESFSSIAENFGIETYHKVLSEFLSVLNCFARGYNGKIFHIYDNMI
AILAKIEEDRFYSLLSAVHIFLRSECIEVDGISFQVKISIGVSSDQSDVYDKARLAMMTASNDSSVEYVSYSEATNKVDR
IYANLKLGRKFNEALNAGNVIPYFQGIYDNKLKRITKYECLARIMEGGQIYNPAMFIPIAKSTGLIRLLTPLMIEKTFKY
FSKHSEYSFSINISESDLDKKGFPLWVMNRLLHYSISPDRVIFEILENDRWNGSSNSTRSLQELKEIGCKIAIDDFGVER
SNFERLMEIQPDFIKIDGKFIQGIHENQTSYRLAAAITEMAHTIGAKVVAKFVSKEEELEAVKSLDIDYSQGYYLMEPAE
EIVYAETVLNLV

Sequences:

>Translated_572_residues
MGNNEFIQNTNLNIKPVILIIDDEVVVLKSIKCAIQLSFGSRFEIEIAEDADSAMEILERCKFEQIDVPLVICDQVLREK
RGDELLIQIHQEYPKIYKVMLTGYASAKTLGNALNKANLYRYLSKPWDSEDLILTISEAVKAYFQNQKVAELSFKLEETY
LFNRETLFPNFENLKRRIDQRLIENENSSLALVRIESFSSIAENFGIETYHKVLSEFLSVLNCFARGYNGKIFHIYDNMI
AILAKIEEDRFYSLLSAVHIFLRSECIEVDGISFQVKISIGVSSDQSDVYDKARLAMMTASNDSSVEYVSYSEATNKVDR
IYANLKLGRKFNEALNAGNVIPYFQGIYDNKLKRITKYECLARIMEGGQIYNPAMFIPIAKSTGLIRLLTPLMIEKTFKY
FSKHSEYSFSINISESDLDKKGFPLWVMNRLLHYSISPDRVIFEILENDRWNGSSNSTRSLQELKEIGCKIAIDDFGVER
SNFERLMEIQPDFIKIDGKFIQGIHENQTSYRLAAAITEMAHTIGAKVVAKFVSKEEELEAVKSLDIDYSQGYYLMEPAE
EIVYAETVLNLV
>Mature_571_residues
GNNEFIQNTNLNIKPVILIIDDEVVVLKSIKCAIQLSFGSRFEIEIAEDADSAMEILERCKFEQIDVPLVICDQVLREKR
GDELLIQIHQEYPKIYKVMLTGYASAKTLGNALNKANLYRYLSKPWDSEDLILTISEAVKAYFQNQKVAELSFKLEETYL
FNRETLFPNFENLKRRIDQRLIENENSSLALVRIESFSSIAENFGIETYHKVLSEFLSVLNCFARGYNGKIFHIYDNMIA
ILAKIEEDRFYSLLSAVHIFLRSECIEVDGISFQVKISIGVSSDQSDVYDKARLAMMTASNDSSVEYVSYSEATNKVDRI
YANLKLGRKFNEALNAGNVIPYFQGIYDNKLKRITKYECLARIMEGGQIYNPAMFIPIAKSTGLIRLLTPLMIEKTFKYF
SKHSEYSFSINISESDLDKKGFPLWVMNRLLHYSISPDRVIFEILENDRWNGSSNSTRSLQELKEIGCKIAIDDFGVERS
NFERLMEIQPDFIKIDGKFIQGIHENQTSYRLAAAITEMAHTIGAKVVAKFVSKEEELEAVKSLDIDYSQGYYLMEPAEE
IVYAETVLNLV

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=404, Percent_Identity=26.980198019802, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1790496, Length=253, Percent_Identity=30.8300395256917, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87082096, Length=230, Percent_Identity=31.7391304347826, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87081845, Length=245, Percent_Identity=28.1632653061224, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI87081743, Length=231, Percent_Identity=26.8398268398268, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI87081921, Length=434, Percent_Identity=23.2718894009217, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787055, Length=276, Percent_Identity=25.7246376811594, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786507, Length=289, Percent_Identity=23.8754325259516, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1787541, Length=420, Percent_Identity=22.8571428571429, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87081980, Length=220, Percent_Identity=25.9090909090909, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788502, Length=245, Percent_Identity=24.8979591836735, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI226510982, Length=235, Percent_Identity=21.2765957446809, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 65596; Mature: 65465

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNNEFIQNTNLNIKPVILIIDDEVVVLKSIKCAIQLSFGSRFEIEIAEDADSAMEILER
CCCCCCEECCCCCEEEEEEEECCCEEEEEEEEEEEEEECCCEEEEEECCCCHHHHHHHHH
CKFEQIDVPLVICDQVLREKRGDELLIQIHQEYPKIYKVMLTGYASAKTLGNALNKANLY
CCCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RYLSKPWDSEDLILTISEAVKAYFQNQKVAELSFKLEETYLFNRETLFPNFENLKRRIDQ
HHHCCCCCCCCEEEEHHHHHHHHHCCCCHHHHHEEEHHHEEECCCCCCCCHHHHHHHHHH
RLIENENSSLALVRIESFSSIAENFGIETYHKVLSEFLSVLNCFARGYNGKIFHIYDNMI
HHHHCCCCCEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHH
AILAKIEEDRFYSLLSAVHIFLRSECIEVDGISFQVKISIGVSSDQSDVYDKARLAMMTA
HHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCEEEEEEEEECCCCCHHHHHHHHEEEEEC
SNDSSVEYVSYSEATNKVDRIYANLKLGRKFNEALNAGNVIPYFQGIYDNKLKRITKYEC
CCCCCEEEEEHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LARIMEGGQIYNPAMFIPIAKSTGLIRLLTPLMIEKTFKYFSKHSEYSFSINISESDLDK
HHHHHCCCCEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
KGFPLWVMNRLLHYSISPDRVIFEILENDRWNGSSNSTRSLQELKEIGCKIAIDDFGVER
CCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCH
SNFERLMEIQPDFIKIDGKFIQGIHENQTSYRLAAAITEMAHTIGAKVVAKFVSKEEELE
HHHHHHHHCCCCEEEECHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AVKSLDIDYSQGYYLMEPAEEIVYAETVLNLV
HHHHCCCCCCCCEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure 
GNNEFIQNTNLNIKPVILIIDDEVVVLKSIKCAIQLSFGSRFEIEIAEDADSAMEILER
CCCCCEECCCCCEEEEEEEECCCEEEEEEEEEEEEEECCCEEEEEECCCCHHHHHHHHH
CKFEQIDVPLVICDQVLREKRGDELLIQIHQEYPKIYKVMLTGYASAKTLGNALNKANLY
CCCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RYLSKPWDSEDLILTISEAVKAYFQNQKVAELSFKLEETYLFNRETLFPNFENLKRRIDQ
HHHCCCCCCCCEEEEHHHHHHHHHCCCCHHHHHEEEHHHEEECCCCCCCCHHHHHHHHHH
RLIENENSSLALVRIESFSSIAENFGIETYHKVLSEFLSVLNCFARGYNGKIFHIYDNMI
HHHHCCCCCEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHH
AILAKIEEDRFYSLLSAVHIFLRSECIEVDGISFQVKISIGVSSDQSDVYDKARLAMMTA
HHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCEEEEEEEEECCCCCHHHHHHHHEEEEEC
SNDSSVEYVSYSEATNKVDRIYANLKLGRKFNEALNAGNVIPYFQGIYDNKLKRITKYEC
CCCCCEEEEEHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LARIMEGGQIYNPAMFIPIAKSTGLIRLLTPLMIEKTFKYFSKHSEYSFSINISESDLDK
HHHHHCCCCEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
KGFPLWVMNRLLHYSISPDRVIFEILENDRWNGSSNSTRSLQELKEIGCKIAIDDFGVER
CCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCH
SNFERLMEIQPDFIKIDGKFIQGIHENQTSYRLAAAITEMAHTIGAKVVAKFVSKEEELE
HHHHHHHHCCCCEEEECHHHHHCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
AVKSLDIDYSQGYYLMEPAEEIVYAETVLNLV
HHHHCCCCCCCCEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]