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The map label for this gene is ycdT [H]

Identifier: 116332510

GI number: 116332510

Start: 74873

End: 76480

Strand: Direct

Name: ycdT [H]

Synonym: LBJ_4068

Alternate gene names: 116332510

Gene position: 74873-76480 (Clockwise)

Preceding gene: 116332509

Following gene: 116332511

Centisome position: 24.98

GC content: 39.12

Gene sequence:

>1608_bases
TTGCAATTCAAAACTTACACGAATGCACAAGTTGCAAACGTAAAAATTAAGAAAAGGATACTCTTTTCGCTCTTGGCAAT
TATTTTTGTATTGCTGTTATTTTTTCTGATTTATGAAAATCTGAGAATGAAAAAAGAAATTGACCGAGTTGTTGGTATGA
GAACCCGCGTGATGAAGGATTACATCCGTAGGGTCGGTTCGCAAACGAGGGCACTTGGGTTATCGATTTCGGATTATTTA
ACCTTTTATGAAGATACATTTGCAAATCCAAGTCTTGTTAAAAAATTCAAAAATTATCCGAATATAAACAGGTTTGGAAT
TCCCCATATCAGTCGAGAAAATAAGGAGGGAGCCGATTCGGGAACTCTTACTGCAATAGGTTCGGTTTATAAATTGAATC
CTTCTCTTCTCAAAGAAATTGAGGCTGCTCTGAACTTAAGAGGACAATTCGAGTCTTTGACTGAAAAACAAAGCGAAGTT
GTTTGGGCTTACTATTTATCTAAACAAAAATTCCTTTATATTACTCCGAAGTTTAAGGATGAAAATTATTATTTTACCGA
TGACCTTTATTTGGGTCCGTATTGGACTCAGGCGACTCCTCAAATCAATCCGACCGGACGTCAAATTATAACCGATCTTT
ACGATGACCTTGCAGGAAAGGGGCTGATGATAACAATTTCCGAACCCGTTTATGTGAATGAGAAATTCATCGGCGTCGCT
TCGATCGATATAGGTTTGGACGCTATGCAAAGAATTTTGGAAAGCGGGGATTGTATCGGAGAAAGTATGCTCATCGATGA
AAATGGAAAAATCATAGCAAAATCCGGAACTTTTAAATTAGATACCAGGCTCCCTCTTCCGGTGATTACCATGATTATGG
AACCGCAGGACTTGTTTTTCCTCGAAAAAGGAAGTTTTTGGATTAGGAACAAAATTAAGGATGAGGAAATTTGGCTCGTT
CATGAAATTAAAATATTTGAATATATTATTTATATAATAAAAAATCTTCTTCCTTTTTGGGTGCTCGTATTTACGTTTTT
TATTGTGCTCATATTATATGTCAAGTTGCGCTCTTCGATGAATCAGGTGGCCAAGTTGATTCATACGGATCCATTGACCG
GGATCTGGAATCGTAGGGGCTTCTTAAAATTAACCCAGAGATCCCTTACCATTGGAAATCGGCACGGAAAGGATTGGACG
ATTTTGCTGGTGGATATCGATCATTTCAAACAGGTGAACGATAAGTTCGGACATGATACCGGAGATAAAACTTTGATAAA
AGTCGCTCAAGTGCTTAGTCGTTCGATTCGCCAAACGGATGCGGTTTGTCGTTGGGGCGGAGAGGAGTTTGCGATCTTTT
TATTCGGAGTCGGTCAGAAGGCTTCTACCGACATCGCCGAAGATCTGCGTAAAGAAGTGGAAAATCAAGTTCGTTTGGAC
GATGGAAACCCGGTGACGTTGAGTATTGGAGTTTCACAAGGAACGTTGGATTTAGAGGAAGCCTTCGTAAATGCAGATCA
GGCGTTGTATCGAGCAAAATCTTCGGGTAGAAACCGGGTTTGTGCTTTTGATCCGAAAGTATTTTCCCATTCAGATTTGA
AGAATTAA

Upstream 100 bases:

>100_bases
TGATAAGTAATGAAAAAGAATTTGGATTTCTTTTTCGATAGAACTTATATCATCGATCATTAAAGCGCTATTATCCATCT
ATGATATTGAGGTGATCAAG

Downstream 100 bases:

>100_bases
ATTGTATCGGAATTTAAATTCGTTTTGGATTTTCTGTAAATCAATAAAAGCTATCTCAAAAATATCTTAGAATGATCTGC
ATCCGCATTTCAAGCAAAGA

Product: GGDEF family protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MQFKTYTNAQVANVKIKKRILFSLLAIIFVLLLFFLIYENLRMKKEIDRVVGMRTRVMKDYIRRVGSQTRALGLSISDYL
TFYEDTFANPSLVKKFKNYPNINRFGIPHISRENKEGADSGTLTAIGSVYKLNPSLLKEIEAALNLRGQFESLTEKQSEV
VWAYYLSKQKFLYITPKFKDENYYFTDDLYLGPYWTQATPQINPTGRQIITDLYDDLAGKGLMITISEPVYVNEKFIGVA
SIDIGLDAMQRILESGDCIGESMLIDENGKIIAKSGTFKLDTRLPLPVITMIMEPQDLFFLEKGSFWIRNKIKDEEIWLV
HEIKIFEYIIYIIKNLLPFWVLVFTFFIVLILYVKLRSSMNQVAKLIHTDPLTGIWNRRGFLKLTQRSLTIGNRHGKDWT
ILLVDIDHFKQVNDKFGHDTGDKTLIKVAQVLSRSIRQTDAVCRWGGEEFAIFLFGVGQKASTDIAEDLRKEVENQVRLD
DGNPVTLSIGVSQGTLDLEEAFVNADQALYRAKSSGRNRVCAFDPKVFSHSDLKN

Sequences:

>Translated_535_residues
MQFKTYTNAQVANVKIKKRILFSLLAIIFVLLLFFLIYENLRMKKEIDRVVGMRTRVMKDYIRRVGSQTRALGLSISDYL
TFYEDTFANPSLVKKFKNYPNINRFGIPHISRENKEGADSGTLTAIGSVYKLNPSLLKEIEAALNLRGQFESLTEKQSEV
VWAYYLSKQKFLYITPKFKDENYYFTDDLYLGPYWTQATPQINPTGRQIITDLYDDLAGKGLMITISEPVYVNEKFIGVA
SIDIGLDAMQRILESGDCIGESMLIDENGKIIAKSGTFKLDTRLPLPVITMIMEPQDLFFLEKGSFWIRNKIKDEEIWLV
HEIKIFEYIIYIIKNLLPFWVLVFTFFIVLILYVKLRSSMNQVAKLIHTDPLTGIWNRRGFLKLTQRSLTIGNRHGKDWT
ILLVDIDHFKQVNDKFGHDTGDKTLIKVAQVLSRSIRQTDAVCRWGGEEFAIFLFGVGQKASTDIAEDLRKEVENQVRLD
DGNPVTLSIGVSQGTLDLEEAFVNADQALYRAKSSGRNRVCAFDPKVFSHSDLKN
>Mature_535_residues
MQFKTYTNAQVANVKIKKRILFSLLAIIFVLLLFFLIYENLRMKKEIDRVVGMRTRVMKDYIRRVGSQTRALGLSISDYL
TFYEDTFANPSLVKKFKNYPNINRFGIPHISRENKEGADSGTLTAIGSVYKLNPSLLKEIEAALNLRGQFESLTEKQSEV
VWAYYLSKQKFLYITPKFKDENYYFTDDLYLGPYWTQATPQINPTGRQIITDLYDDLAGKGLMITISEPVYVNEKFIGVA
SIDIGLDAMQRILESGDCIGESMLIDENGKIIAKSGTFKLDTRLPLPVITMIMEPQDLFFLEKGSFWIRNKIKDEEIWLV
HEIKIFEYIIYIIKNLLPFWVLVFTFFIVLILYVKLRSSMNQVAKLIHTDPLTGIWNRRGFLKLTQRSLTIGNRHGKDWT
ILLVDIDHFKQVNDKFGHDTGDKTLIKVAQVLSRSIRQTDAVCRWGGEEFAIFLFGVGQKASTDIAEDLRKEVENQVRLD
DGNPVTLSIGVSQGTLDLEEAFVNADQALYRAKSSGRNRVCAFDPKVFSHSDLKN

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=195, Percent_Identity=35.3846153846154, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87081881, Length=163, Percent_Identity=38.6503067484663, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87082007, Length=429, Percent_Identity=27.039627039627, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786584, Length=156, Percent_Identity=38.4615384615385, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI145693134, Length=171, Percent_Identity=36.2573099415205, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=34.8066298342541, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787802, Length=174, Percent_Identity=36.7816091954023, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI87081974, Length=338, Percent_Identity=24.2603550295858, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788381, Length=184, Percent_Identity=28.2608695652174, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081977, Length=176, Percent_Identity=27.8409090909091, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787816, Length=194, Percent_Identity=30.9278350515464, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787541, Length=176, Percent_Identity=26.1363636363636, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788956, Length=166, Percent_Identity=28.9156626506024, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 61387; Mature: 61387

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFKTYTNAQVANVKIKKRILFSLLAIIFVLLLFFLIYENLRMKKEIDRVVGMRTRVMKD
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIRRVGSQTRALGLSISDYLTFYEDTFANPSLVKKFKNYPNINRFGIPHISRENKEGADS
HHHHHCCCHHEECCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GTLTAIGSVYKLNPSLLKEIEAALNLRGQFESLTEKQSEVVWAYYLSKQKFLYITPKFKD
CCEEEECCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHCEEEEEEECCCEEEEEEECEEC
ENYYFTDDLYLGPYWTQATPQINPTGRQIITDLYDDLAGKGLMITISEPVYVNEKFIGVA
CCEEEECCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEECCEEEEEE
SIDIGLDAMQRILESGDCIGESMLIDENGKIIAKSGTFKLDTRLPLPVITMIMEPQDLFF
EECCCHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCEEEECCCCHHHHHHHCCCCCEEE
LEKGSFWIRNKIKDEEIWLVHEIKIFEYIIYIIKNLLPFWVLVFTFFIVLILYVKLRSSM
EECCCEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQVAKLIHTDPLTGIWNRRGFLKLTQRSLTIGNRHGKDWTILLVDIDHFKQVNDKFGHDT
HHHHHHHHCCCCCHHHCCCCCEEEHHHHEEECCCCCCCEEEEEEEHHHHHHHHHHCCCCC
GDKTLIKVAQVLSRSIRQTDAVCRWGGEEFAIFLFGVGQKASTDIAEDLRKEVENQVRLD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCEEEC
DGNPVTLSIGVSQGTLDLEEAFVNADQALYRAKSSGRNRVCAFDPKVFSHSDLKN
CCCEEEEEEECCCCCCHHHHHHHCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MQFKTYTNAQVANVKIKKRILFSLLAIIFVLLLFFLIYENLRMKKEIDRVVGMRTRVMKD
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIRRVGSQTRALGLSISDYLTFYEDTFANPSLVKKFKNYPNINRFGIPHISRENKEGADS
HHHHHCCCHHEECCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GTLTAIGSVYKLNPSLLKEIEAALNLRGQFESLTEKQSEVVWAYYLSKQKFLYITPKFKD
CCEEEECCEEECCHHHHHHHHHHHCCCCHHHHHHHHHHCEEEEEEECCCEEEEEEECEEC
ENYYFTDDLYLGPYWTQATPQINPTGRQIITDLYDDLAGKGLMITISEPVYVNEKFIGVA
CCEEEECCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEECCEEEEEE
SIDIGLDAMQRILESGDCIGESMLIDENGKIIAKSGTFKLDTRLPLPVITMIMEPQDLFF
EECCCHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCEEEECCCCHHHHHHHCCCCCEEE
LEKGSFWIRNKIKDEEIWLVHEIKIFEYIIYIIKNLLPFWVLVFTFFIVLILYVKLRSSM
EECCCEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQVAKLIHTDPLTGIWNRRGFLKLTQRSLTIGNRHGKDWTILLVDIDHFKQVNDKFGHDT
HHHHHHHHCCCCCHHHCCCCCEEEHHHHEEECCCCCCCEEEEEEEHHHHHHHHHHCCCCC
GDKTLIKVAQVLSRSIRQTDAVCRWGGEEFAIFLFGVGQKASTDIAEDLRKEVENQVRLD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCEEEC
DGNPVTLSIGVSQGTLDLEEAFVNADQALYRAKSSGRNRVCAFDPKVFSHSDLKN
CCCEEEEEEECCCCCCHHHHHHHCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]