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The map label for this gene is pntA [H]

Identifier: 116329519

GI number: 116329519

Start: 3564560

End: 3565729

Strand: Direct

Name: pntA [H]

Synonym: LBL_2994

Alternate gene names: 116329519

Gene position: 3564560-3565729 (Clockwise)

Preceding gene: 116329518

Following gene: 116329524

Centisome position: 98.62

GC content: 41.62

Gene sequence:

>1170_bases
ATGAATATTGGAATTCTAAAAGAAGCTAAAGAAGAAACGAGAGTATCCGTAACTCCCGACATCGTAGATACATTAAAGAA
AATTGGGGCGGTTGTCCTCGTTGAAAAAGGTGCTGGGGAACAATCTTATTATCATGATGAAGATTATAAAAAAGCCGGAG
CAAATCTTGTATCTCGCCAAGATATTATTGGTAAATCCAATATTATAGTGAGTATTCATCTTGCGGATCCTGCAACATTA
GCAAAGATTAAACCGGGAACGATTTATCTCGGGATGTTCCAGCCCGCCATAAATGCATCAACCATCCAAAAACTCGCGGC
TAAAAAAGTGGAAGTTTTAAGTTTGGATGCGATCGCAAGAATTACAAGAGCTCAGTCGATGGATGTTCTTTCTTCCCAAG
CGACCGTTGCCGGCTACAAAGCAGTGCTACTTGCGGCTTCTCATTTGGCTAGATTTTTCCCAATGCTTACGACTGCAGCT
GGAACGATCACACCTGCTTCTGTGTTGATTATTGGAGCCGGAGTCGCAGGTTTACAAGCGATCGCTTCTAGTAGAAGGCT
GGGTGCGGTAGTGGATGTATTTGATACAAGACCGGAAGTAAAAGAGCAAGTTCATTCTCTTGGCGCAAAGTTCGTGGAAG
TGGAAGGCGCCTCGCATTCCGCTGCGGCCGGTGGTTACGCAGTAGAACAGACTGAAGAATACAAAAAGCGTCAGCAAGAA
GCCATCGATAAATACGCTCAGAAGGCGGACGTAATCATAACGACTGCATTGATTCCGGGAAAAAAAGCCCCTTTACTAAT
TACGAAGAAGATTGTGGATAATATGAAATCCGGTTCCGTAATTGTGGATCTTGCGTCTTCCATGGGAGGAAATTGCGAAT
ATACAAAACATGGACAAAATGTAATCACTTCGAAAGGGGTAACGGTAATCGGTCATAAAAATCTTGCGGGCTCGATTCCT
GCGGATGCTTCCAAAATGTTCGGTAAAAACGTTTTAAACTTTCTTAAACTATTGATTAAAGATAAAAAGATTAATCACGA
TTTGAGTGACGAAATACTTTCTTCCACAACGATCGTTCATAAGGGAGAAATTCGTCATAAACTTACGTTAGATGCTTTAG
GTTCGAAGTTAGGAGCAACAAAATCTAAGAAGAACGTTACCAAGAAATAA

Upstream 100 bases:

>100_bases
ACTTCAAAAATAACTTGGATTAAATTTATACAGAGAAATTCTGGACGTACTTTATTAAGCGAGTCCGGAACAACGAGCTA
TTGATCACCGGGAATGAATT

Downstream 100 bases:

>100_bases
ACCGAAAAAACCGAAAGCTGCTCCAATCATCCAGCCGCCGATTATATCGCTTACGAAATGATGGAGAGAAAGCAACCTTC
CGACTCCGGCTAAAAAACTG

Product: NAD(P)(+) transhydrogenase (AB-specific), alpha subunit

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MNIGILKEAKEETRVSVTPDIVDTLKKIGAVVLVEKGAGEQSYYHDEDYKKAGANLVSRQDIIGKSNIIVSIHLADPATL
AKIKPGTIYLGMFQPAINASTIQKLAAKKVEVLSLDAIARITRAQSMDVLSSQATVAGYKAVLLAASHLARFFPMLTTAA
GTITPASVLIIGAGVAGLQAIASSRRLGAVVDVFDTRPEVKEQVHSLGAKFVEVEGASHSAAAGGYAVEQTEEYKKRQQE
AIDKYAQKADVIITTALIPGKKAPLLITKKIVDNMKSGSVIVDLASSMGGNCEYTKHGQNVITSKGVTVIGHKNLAGSIP
ADASKMFGKNVLNFLKLLIKDKKINHDLSDEILSSTTIVHKGEIRHKLTLDALGSKLGATKSKKNVTKK

Sequences:

>Translated_389_residues
MNIGILKEAKEETRVSVTPDIVDTLKKIGAVVLVEKGAGEQSYYHDEDYKKAGANLVSRQDIIGKSNIIVSIHLADPATL
AKIKPGTIYLGMFQPAINASTIQKLAAKKVEVLSLDAIARITRAQSMDVLSSQATVAGYKAVLLAASHLARFFPMLTTAA
GTITPASVLIIGAGVAGLQAIASSRRLGAVVDVFDTRPEVKEQVHSLGAKFVEVEGASHSAAAGGYAVEQTEEYKKRQQE
AIDKYAQKADVIITTALIPGKKAPLLITKKIVDNMKSGSVIVDLASSMGGNCEYTKHGQNVITSKGVTVIGHKNLAGSIP
ADASKMFGKNVLNFLKLLIKDKKINHDLSDEILSSTTIVHKGEIRHKLTLDALGSKLGATKSKKNVTKK
>Mature_389_residues
MNIGILKEAKEETRVSVTPDIVDTLKKIGAVVLVEKGAGEQSYYHDEDYKKAGANLVSRQDIIGKSNIIVSIHLADPATL
AKIKPGTIYLGMFQPAINASTIQKLAAKKVEVLSLDAIARITRAQSMDVLSSQATVAGYKAVLLAASHLARFFPMLTTAA
GTITPASVLIIGAGVAGLQAIASSRRLGAVVDVFDTRPEVKEQVHSLGAKFVEVEGASHSAAAGGYAVEQTEEYKKRQQE
AIDKYAQKADVIITTALIPGKKAPLLITKKIVDNMKSGSVIVDLASSMGGNCEYTKHGQNVITSKGVTVIGHKNLAGSIP
ADASKMFGKNVLNFLKLLIKDKKINHDLSDEILSSTTIVHKGEIRHKLTLDALGSKLGATKSKKNVTKK

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=362, Percent_Identity=39.7790055248619, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI122939153, Length=362, Percent_Identity=39.7790055248619, Blast_Score=247, Evalue=2e-65,
Organism=Escherichia coli, GI1787887, Length=367, Percent_Identity=45.5040871934605, Blast_Score=326, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17550456, Length=355, Percent_Identity=40.8450704225352, Blast_Score=266, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 41373; Mature: 41373

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIGILKEAKEETRVSVTPDIVDTLKKIGAVVLVEKGAGEQSYYHDEDYKKAGANLVSRQ
CCCCCCHHHHHHCEEEECHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHCCHHHHHH
DIIGKSNIIVSIHLADPATLAKIKPGTIYLGMFQPAINASTIQKLAAKKVEVLSLDAIAR
HHCCCCCEEEEEEECCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
ITRAQSMDVLSSQATVAGYKAVLLAASHLARFFPMLTTAAGTITPASVLIIGAGVAGLQA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHH
IASSRRLGAVVDVFDTRPEVKEQVHSLGAKFVEVEGASHSAAAGGYAVEQTEEYKKRQQE
HHHCCCCCCEEEHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
AIDKYAQKADVIITTALIPGKKAPLLITKKIVDNMKSGSVIVDLASSMGGNCEYTKHGQN
HHHHHHHHCCEEEEEEECCCCCCCEEEEHHHHCCCCCCCEEEEEHHHCCCCCEECCCCCE
VITSKGVTVIGHKNLAGSIPADASKMFGKNVLNFLKLLIKDKKINHDLSDEILSSTTIVH
EEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCEEEE
KGEIRHKLTLDALGSKLGATKSKKNVTKK
CCCCCCEEEHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNIGILKEAKEETRVSVTPDIVDTLKKIGAVVLVEKGAGEQSYYHDEDYKKAGANLVSRQ
CCCCCCHHHHHHCEEEECHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHCCHHHHHH
DIIGKSNIIVSIHLADPATLAKIKPGTIYLGMFQPAINASTIQKLAAKKVEVLSLDAIAR
HHCCCCCEEEEEEECCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
ITRAQSMDVLSSQATVAGYKAVLLAASHLARFFPMLTTAAGTITPASVLIIGAGVAGLQA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHH
IASSRRLGAVVDVFDTRPEVKEQVHSLGAKFVEVEGASHSAAAGGYAVEQTEEYKKRQQE
HHHCCCCCCEEEHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
AIDKYAQKADVIITTALIPGKKAPLLITKKIVDNMKSGSVIVDLASSMGGNCEYTKHGQN
HHHHHHHHCCEEEEEEECCCCCCCEEEEHHHHCCCCCCCEEEEEHHHCCCCCEECCCCCE
VITSKGVTVIGHKNLAGSIPADASKMFGKNVLNFLKLLIKDKKINHDLSDEILSSTTIVH
EEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCEEEE
KGEIRHKLTLDALGSKLGATKSKKNVTKK
CCCCCCEEEHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]