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The map label for this gene is ybjS [C]

Identifier: 116329365

GI number: 116329365

Start: 3360642

End: 3361607

Strand: Direct

Name: ybjS [C]

Synonym: LBL_2821

Alternate gene names: 116329365

Gene position: 3360642-3361607 (Clockwise)

Preceding gene: 116329364

Following gene: 116329366

Centisome position: 92.98

GC content: 43.89

Gene sequence:

>966_bases
ATGAATCTTTTTATCACAGGAGCTTCCGGCTTTGTAGGAGAAGCGGCGACTCGAATTTTATCTAAAAAACATATTGTTAA
AGCAATGTCCCGGTCGGAAAAAGCAGACGCAGTCATTTCCATGGTAGGAGGAAAGCCTGTTCGCTGTGAACTCAATTCCG
TGGATTCAAATTCTTTGAAAGGAATCGACGTCGTCATTCACAGCGCTGCTTATGTGGAACAATGGGGACCGCTTCGGGAT
TTTTGGAAGGTCAACGTGGACGGAACCGCTCAACTTCTGGAGGCGTCTCGCAAAGCAGGAGTCAAACGATTCATCTTTAT
CGGAACCGAAGCCGCTCTCTTTTACGGACAACCGATGATCGACATTGACGAATCGTATCCTTATCCGAAAAATTCTCCCT
TTCCTTATTCAAAGACCAAAGCCGAAGCGGAGAAGCTCGTATTAAAAGCGAATTCCTCTGAAATGCAAACTATTTCCATC
CGTCCTCGTTTGATTTGGGGGCCCGGCGATAAAACGGTTCTCCCGGTTCTTTTAAAGATGATCTCAGATGGGAATTTTTC
TTGGATCGACGGCGGCAGGGCTCTTACTAGTACGACTCACATTTACAATCTAATTCATTCAATCGAACTCGCTCTTACAA
AAGGCCAAGGCGGTAAGGCTTATTTTGTCACCGACGATGAAGTATTCAATTTTCGTAATTTTCTTGAATCTCTTCTCGAA
ACTCAGAAAGTGGTAGCTCCGAATCGTTCTATCCCGGGTTGGCTTGCTCGTTTTTTAGCGAGAATTCTAGAAAGAGTTTG
GAAACTTTTTAGAATCAAAAACGAACCTCCTCTAACTCGCTTTAGCGCGAGTATCATGTCTCGAGATTGTACAATCAAAA
TCGATAATGCCAAAAAAGATTTAGATTATTCTCCCCTGCTGACGGTTCGCCAAGGTCTTGCAGAAATGCCGGTTCTTGGT
TCTTAA

Upstream 100 bases:

>100_bases
ACTGGAAAATATTTTGAATATTCTAACTTAAGAGAATGTAATTGACCGCTTTTATTTTCCAATAAAATCAGTAGATCCAT
CCATTTCTAGGGAGCGAAGA

Downstream 100 bases:

>100_bases
ATGTCGGAATTTTTCGATAGGATGTAGGAACTTCTAAAAAATTGAACAACGAAAAAACTGCTCGAAAACCCATAAGTTCT
ATCTCAGGGAAAATAATTTG

Product: 3-beta hydroxysteroid dehydrogenase/isomerase family protein

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNLFITGASGFVGEAATRILSKKHIVKAMSRSEKADAVISMVGGKPVRCELNSVDSNSLKGIDVVIHSAAYVEQWGPLRD
FWKVNVDGTAQLLEASRKAGVKRFIFIGTEAALFYGQPMIDIDESYPYPKNSPFPYSKTKAEAEKLVLKANSSEMQTISI
RPRLIWGPGDKTVLPVLLKMISDGNFSWIDGGRALTSTTHIYNLIHSIELALTKGQGGKAYFVTDDEVFNFRNFLESLLE
TQKVVAPNRSIPGWLARFLARILERVWKLFRIKNEPPLTRFSASIMSRDCTIKIDNAKKDLDYSPLLTVRQGLAEMPVLG
S

Sequences:

>Translated_321_residues
MNLFITGASGFVGEAATRILSKKHIVKAMSRSEKADAVISMVGGKPVRCELNSVDSNSLKGIDVVIHSAAYVEQWGPLRD
FWKVNVDGTAQLLEASRKAGVKRFIFIGTEAALFYGQPMIDIDESYPYPKNSPFPYSKTKAEAEKLVLKANSSEMQTISI
RPRLIWGPGDKTVLPVLLKMISDGNFSWIDGGRALTSTTHIYNLIHSIELALTKGQGGKAYFVTDDEVFNFRNFLESLLE
TQKVVAPNRSIPGWLARFLARILERVWKLFRIKNEPPLTRFSASIMSRDCTIKIDNAKKDLDYSPLLTVRQGLAEMPVLG
S
>Mature_321_residues
MNLFITGASGFVGEAATRILSKKHIVKAMSRSEKADAVISMVGGKPVRCELNSVDSNSLKGIDVVIHSAAYVEQWGPLRD
FWKVNVDGTAQLLEASRKAGVKRFIFIGTEAALFYGQPMIDIDESYPYPKNSPFPYSKTKAEAEKLVLKANSSEMQTISI
RPRLIWGPGDKTVLPVLLKMISDGNFSWIDGGRALTSTTHIYNLIHSIELALTKGQGGKAYFVTDDEVFNFRNFLESLLE
TQKVVAPNRSIPGWLARFLARILERVWKLFRIKNEPPLTRFSASIMSRDCTIKIDNAKKDLDYSPLLTVRQGLAEMPVLG
S

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=322, Percent_Identity=27.9503105590062, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI8393516, Length=322, Percent_Identity=27.9503105590062, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI116268111, Length=332, Percent_Identity=30.421686746988, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI310132178, Length=277, Percent_Identity=29.2418772563177, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI310113012, Length=277, Percent_Identity=29.2418772563177, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI239745448, Length=277, Percent_Identity=29.2418772563177, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI4504507, Length=348, Percent_Identity=25, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI19923621, Length=337, Percent_Identity=22.8486646884273, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI260763931, Length=341, Percent_Identity=23.7536656891496, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4504509, Length=341, Percent_Identity=23.7536656891496, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI87081792, Length=325, Percent_Identity=28.6153846153846, Blast_Score=134, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI193211272, Length=331, Percent_Identity=26.2839879154079, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17570557, Length=278, Percent_Identity=28.0575539568345, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17509709, Length=277, Percent_Identity=28.158844765343, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321437, Length=273, Percent_Identity=27.4725274725275, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 35717; Mature: 35717

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFITGASGFVGEAATRILSKKHIVKAMSRSEKADAVISMVGGKPVRCELNSVDSNSLK
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCC
GIDVVIHSAAYVEQWGPLRDFWKVNVDGTAQLLEASRKAGVKRFIFIGTEAALFYGQPMI
CCCEEEHHHHHHHHCCCCCHHEEECCCCHHHHHHHHHHCCCEEEEEEECCCCEEECCCCE
DIDESYPYPKNSPFPYSKTKAEAEKLVLKANSSEMQTISIRPRLIWGPGDKTVLPVLLKM
ECCCCCCCCCCCCCCCCHHHHHHHHEEEEECCCCEEEEEECEEEEECCCCCHHHHHHHHH
ISDGNFSWIDGGRALTSTTHIYNLIHSIELALTKGQGGKAYFVTDDEVFNFRNFLESLLE
HCCCCEEEECCCCHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECHHHHHHHHHHHHHHH
TQKVVAPNRSIPGWLARFLARILERVWKLFRIKNEPPLTRFSASIMSRDCTIKIDNAKKD
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCCCCC
LDYSPLLTVRQGLAEMPVLGS
CCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNLFITGASGFVGEAATRILSKKHIVKAMSRSEKADAVISMVGGKPVRCELNSVDSNSLK
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCC
GIDVVIHSAAYVEQWGPLRDFWKVNVDGTAQLLEASRKAGVKRFIFIGTEAALFYGQPMI
CCCEEEHHHHHHHHCCCCCHHEEECCCCHHHHHHHHHHCCCEEEEEEECCCCEEECCCCE
DIDESYPYPKNSPFPYSKTKAEAEKLVLKANSSEMQTISIRPRLIWGPGDKTVLPVLLKM
ECCCCCCCCCCCCCCCCHHHHHHHHEEEEECCCCEEEEEECEEEEECCCCCHHHHHHHHH
ISDGNFSWIDGGRALTSTTHIYNLIHSIELALTKGQGGKAYFVTDDEVFNFRNFLESLLE
HCCCCEEEECCCCHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECHHHHHHHHHHHHHHH
TQKVVAPNRSIPGWLARFLARILERVWKLFRIKNEPPLTRFSASIMSRDCTIKIDNAKKD
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCCCCC
LDYSPLLTVRQGLAEMPVLGS
CCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]