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The map label for this gene is gmd [H]

Identifier: 116329230

GI number: 116329230

Start: 3161270

End: 3162292

Strand: Direct

Name: gmd [H]

Synonym: LBL_2667

Alternate gene names: 116329230

Gene position: 3161270-3162292 (Clockwise)

Preceding gene: 116329229

Following gene: 116329231

Centisome position: 87.46

GC content: 45.06

Gene sequence:

>1023_bases
ATGAAAAAAGCGCTCATAACCGGAATCACAGGACAGGACGGCTCTTATCTAACGGAATTTTTACTCCAGAAAGGGTATCA
AGTGCACGGAATTGTCAGAAGATCCAGTATGTTCAACCGAGCAAGGATTGAACACCTTCGTGGGAATTCCAATCTAATTC
TGCATTACGGGGATCTTACCGACTCAAGCAATTTAAATCGAATTTTGGAAAAAGTTTCACCGGACGAAATTTACAATCTC
GCCGCTCAATCTCATGTGGGAGTTTCTTTTGAAGTTCCGGAATATACCGCAGAAGCCGACGCGGTGGGAACGCTTAGAAT
TTTAGACGCGATCAAACAGATTGGAGTGAAAAGCAGATTTTATCAGGCGTCCACATCCGAACTTTACGGAAAGGTACAGG
CAATTCCACAGACAGAAACGACTCCATTCTATCCAAGATCTCCTTATGCAGTCGCAAAGTTATATGCATACTGGGCGGTC
GTAAATTATAGAGAGGCATTTGAAATCCATGCATCCAATGGAATCCTATTCAACCACGAATCCCCAAGACGAGGAGAAGG
TTTTGTAACTAGAAAGATCACCATCGGAGTCGCCAATCTGCTCGCGAAAAAAGGCGGACCGATTCAACTCGGAAACATGG
ATGCAAAAAGGGACTGGGGATACGCGCCGGACTACGTCGAAATGATGTGGATGATGCTCCAGCAACCCGAAGCGGACGAT
TACGTCGTGGCGACCAATGAAACACATACCGTAAGGGAGTTCGTGGAAAAATCTTTTGGATTCGTCGGAATTCAAGTACG
TTGGGAAGGGAAAGGAGATTCCGAGAAAGGATTCGATGCAAAGTCCGGGCAACTCCTCGTGGAGGTGAATCCTAAATTCT
ATCGCCCGACCGAAGTCGATATTTTGATCGGCGATCCTTCTAAAGCGAAGAAAAAACTCGGTTGGGAGCCGAAAGTAAAA
TTCGAAGAGCTCGTTAGAATCATGATCAAAGCGGATTGTGAATTAGTCGGAATAAAACTTTAA

Upstream 100 bases:

>100_bases
TCGCTTCCTATGGGGCACTCTGCAGGTGAGAATTTCCACAACATAATAGGCTTTTAAACTTTCTTGCCAGATCGGGTTTT
CGGGTTTTCCTGGTCTGAAG

Downstream 100 bases:

>100_bases
AAATACATTCTAATTTTCGAATTGAACAAAGAAAACAAATAGGACGAACTTTCCTGCTTCCTTATCCCAATCCGATGAAC
ACTACTTTCTTTAAAGAGAA

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKKALITGITGQDGSYLTEFLLQKGYQVHGIVRRSSMFNRARIEHLRGNSNLILHYGDLTDSSNLNRILEKVSPDEIYNL
AAQSHVGVSFEVPEYTAEADAVGTLRILDAIKQIGVKSRFYQASTSELYGKVQAIPQTETTPFYPRSPYAVAKLYAYWAV
VNYREAFEIHASNGILFNHESPRRGEGFVTRKITIGVANLLAKKGGPIQLGNMDAKRDWGYAPDYVEMMWMMLQQPEADD
YVVATNETHTVREFVEKSFGFVGIQVRWEGKGDSEKGFDAKSGQLLVEVNPKFYRPTEVDILIGDPSKAKKKLGWEPKVK
FEELVRIMIKADCELVGIKL

Sequences:

>Translated_340_residues
MKKALITGITGQDGSYLTEFLLQKGYQVHGIVRRSSMFNRARIEHLRGNSNLILHYGDLTDSSNLNRILEKVSPDEIYNL
AAQSHVGVSFEVPEYTAEADAVGTLRILDAIKQIGVKSRFYQASTSELYGKVQAIPQTETTPFYPRSPYAVAKLYAYWAV
VNYREAFEIHASNGILFNHESPRRGEGFVTRKITIGVANLLAKKGGPIQLGNMDAKRDWGYAPDYVEMMWMMLQQPEADD
YVVATNETHTVREFVEKSFGFVGIQVRWEGKGDSEKGFDAKSGQLLVEVNPKFYRPTEVDILIGDPSKAKKKLGWEPKVK
FEELVRIMIKADCELVGIKL
>Mature_340_residues
MKKALITGITGQDGSYLTEFLLQKGYQVHGIVRRSSMFNRARIEHLRGNSNLILHYGDLTDSSNLNRILEKVSPDEIYNL
AAQSHVGVSFEVPEYTAEADAVGTLRILDAIKQIGVKSRFYQASTSELYGKVQAIPQTETTPFYPRSPYAVAKLYAYWAV
VNYREAFEIHASNGILFNHESPRRGEGFVTRKITIGVANLLAKKGGPIQLGNMDAKRDWGYAPDYVEMMWMMLQQPEADD
YVVATNETHTVREFVEKSFGFVGIQVRWEGKGDSEKGFDAKSGQLLVEVNPKFYRPTEVDILIGDPSKAKKKLGWEPKVK
FEELVRIMIKADCELVGIKL

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=341, Percent_Identity=63.6363636363636, Blast_Score=446, Evalue=1e-125,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1788366, Length=350, Percent_Identity=59.1428571428571, Blast_Score=430, Evalue=1e-122,
Organism=Escherichia coli, GI48994969, Length=362, Percent_Identity=25.9668508287293, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788353, Length=358, Percent_Identity=24.3016759776536, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786974, Length=347, Percent_Identity=25.6484149855908, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=61.6279069767442, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17539424, Length=344, Percent_Identity=60.7558139534884, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17539422, Length=344, Percent_Identity=60.7558139534884, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI133901788, Length=344, Percent_Identity=60.7558139534884, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI133901790, Length=344, Percent_Identity=60.7558139534884, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI133901786, Length=344, Percent_Identity=60.7558139534884, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17539532, Length=332, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24158427, Length=343, Percent_Identity=62.6822157434402, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI21356223, Length=330, Percent_Identity=26.6666666666667, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 38324; Mature: 38324

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALITGITGQDGSYLTEFLLQKGYQVHGIVRRSSMFNRARIEHLRGNSNLILHYGDLT
CCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCC
DSSNLNRILEKVSPDEIYNLAAQSHVGVSFEVPEYTAEADAVGTLRILDAIKQIGVKSRF
CCCHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHH
YQASTSELYGKVQAIPQTETTPFYPRSPYAVAKLYAYWAVVNYREAFEIHASNGILFNHE
HCCHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCEEECCC
SPRRGEGFVTRKITIGVANLLAKKGGPIQLGNMDAKRDWGYAPDYVEMMWMMLQQPEADD
CCCCCCCEEEEEEEHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
YVVATNETHTVREFVEKSFGFVGIQVRWEGKGDSEKGFDAKSGQLLVEVNPKFYRPTEVD
EEEEECCCHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEECCCEEE
ILIGDPSKAKKKLGWEPKVKFEELVRIMIKADCELVGIKL
EEECCCHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEC
>Mature Secondary Structure
MKKALITGITGQDGSYLTEFLLQKGYQVHGIVRRSSMFNRARIEHLRGNSNLILHYGDLT
CCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCC
DSSNLNRILEKVSPDEIYNLAAQSHVGVSFEVPEYTAEADAVGTLRILDAIKQIGVKSRF
CCCHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHH
YQASTSELYGKVQAIPQTETTPFYPRSPYAVAKLYAYWAVVNYREAFEIHASNGILFNHE
HCCHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCEEECCC
SPRRGEGFVTRKITIGVANLLAKKGGPIQLGNMDAKRDWGYAPDYVEMMWMMLQQPEADD
CCCCCCCEEEEEEEHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
YVVATNETHTVREFVEKSFGFVGIQVRWEGKGDSEKGFDAKSGQLLVEVNPKFYRPTEVD
EEEEECCCHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEECCCEEE
ILIGDPSKAKKKLGWEPKVKFEELVRIMIKADCELVGIKL
EEECCCHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]