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The map label for this gene is murE

Identifier: 116329173

GI number: 116329173

Start: 3074726

End: 3076228

Strand: Direct

Name: murE

Synonym: LBL_2600

Alternate gene names: 116329173

Gene position: 3074726-3076228 (Clockwise)

Preceding gene: 116329172

Following gene: 116329174

Centisome position: 85.07

GC content: 44.84

Gene sequence:

>1503_bases
ATGAAAATGAAGTTAACCAATCTTCTCCATGAATTTCCGGAACTCAAATTAGGATCACTTCCTTCGGGAAAAAATCCGGA
TTCGGTTCCGATCGAATACATCCAATCGGATTCCAGAAAAACGAATCCAGAGGACATCTTCTGCGTTCCGGAATCAATCG
GCACTAAAAAAGGAGAATTTATCTCCAACACCAAAGCTTCCGTGATTTTACTACATCGCAGTTCTAACATCTCCATCGAT
TCCTCTAAAATCGTTTTGGAATGCGAGGAAGATCCAGAACAACTCCAAGGCAGAATCGCTTCTTTTCTGCTCGGTCATCC
TTCGAAAACTTTGGAAATCGTAGCGGTAACCGGCACAAACGGTAAAACTTCTCTGACAAATATCCTATTCGCATTAGCAA
AAGATCAGGGAAAAATCTGCGGATTGATCGGAACGATCGGCGTTAAATTCGGAGATCGATCGATCGATACCGGTTATACG
ACTCCGGACGCTTCCTCCCTCAATCTCATTCTAAAGCAGATGAAAGACGAAGGAGTAACGACCGTGTTTATGGAAGCGAG
TTCCCACGGTCTTAAACTCGGGAGAATCGGCGGAATCTCTTTAAAGGCGGGAGTATTTACCAATCTGACTCAGGATCACT
TAGACTTTCATTCCGATATGGAAGATTATTTTGAAAGTAAGTTCCGTCTTTTTGAAATCTTGGACGTTTCAAAATCCCCC
TTTGCGGTTTTGGATTACTCTTCTCCGGGTGGAAATGAACTCCATCGGAAAATCAGAAATAACCTTCCGGATTTACCGAT
AAAAGCGTTAGACGGCATTGGAGGAGAATATAAGGTGAGCAATCCCTTCCTTACCTTACAAGGGACTTCCTACGTCTTAA
GTTTGCCGGGAAACCGTAGTCAAACGATTTCCACCAATCTTCTCGGTTCGTTTAACGTTCGAAACACTGCACTTGCGTTT
TTAACGGGACTCGGTCTCGGTTTAGACCCCAAAGGGATGTTTTCTTCTTTGAAAGAAATTCCGCAAATTCCGGGTAGGTT
TCAGATCGTTTACAGCAAAGATCGCTCTCGGATGGCGGTCGTAGATTACGCACACACTCCGGACGCCCTCGAAAATATAA
TTCGAAGTGTAAGGAATTCCAGGCCGAAACGTTTGATTACCCTATTCGGATGTGGAGGCGATCGAGACAAAACCAAACGT
CCTAAGATGGCGCGTATCGCAGAGGAACTTTCCGATCAAGTAATATTGACCTCGGATAACCCGAGGTCAGAAAAACCGGA
AGCCATTTTGGACGAGATACAATCCGGGTTTTCTCCCGGGTTTACTCCACTTTTGCGGGAAGTCGATCGGGCCCGAGCGA
TCTCGGAAGGAGTCGGTATTTTACCAGAAGGAGGTTGTTTGCTCGTAGCCGGAAAAGGACATGAAGAATACCAGATCATC
GGAAAAGAAAAACGTCATTTCAGCGATGTGGAAGAAGTCCGAAAAGCTTTCGGCCTTTTTTAG

Upstream 100 bases:

>100_bases
AGAAGAATCGAAACTCTTTATAGAAAAACATACAACTATCTTCCCATCACCGTGGGAGATAGAATCATCACTTTGAATTT
ACCTCCCGAGAAGAACGAAG

Downstream 100 bases:

>100_bases
AAATTTTCCGATACAAAAAAAGAATACTTCGTTTTCTTGAAAGCAGGACGGACAAAAATGTTTTATTATCTATACGATCT
TTACTTTAACCATCTCGATT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2,6- diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MKMKLTNLLHEFPELKLGSLPSGKNPDSVPIEYIQSDSRKTNPEDIFCVPESIGTKKGEFISNTKASVILLHRSSNISID
SSKIVLECEEDPEQLQGRIASFLLGHPSKTLEIVAVTGTNGKTSLTNILFALAKDQGKICGLIGTIGVKFGDRSIDTGYT
TPDASSLNLILKQMKDEGVTTVFMEASSHGLKLGRIGGISLKAGVFTNLTQDHLDFHSDMEDYFESKFRLFEILDVSKSP
FAVLDYSSPGGNELHRKIRNNLPDLPIKALDGIGGEYKVSNPFLTLQGTSYVLSLPGNRSQTISTNLLGSFNVRNTALAF
LTGLGLGLDPKGMFSSLKEIPQIPGRFQIVYSKDRSRMAVVDYAHTPDALENIIRSVRNSRPKRLITLFGCGGDRDKTKR
PKMARIAEELSDQVILTSDNPRSEKPEAILDEIQSGFSPGFTPLLREVDRARAISEGVGILPEGGCLLVAGKGHEEYQII
GKEKRHFSDVEEVRKAFGLF

Sequences:

>Translated_500_residues
MKMKLTNLLHEFPELKLGSLPSGKNPDSVPIEYIQSDSRKTNPEDIFCVPESIGTKKGEFISNTKASVILLHRSSNISID
SSKIVLECEEDPEQLQGRIASFLLGHPSKTLEIVAVTGTNGKTSLTNILFALAKDQGKICGLIGTIGVKFGDRSIDTGYT
TPDASSLNLILKQMKDEGVTTVFMEASSHGLKLGRIGGISLKAGVFTNLTQDHLDFHSDMEDYFESKFRLFEILDVSKSP
FAVLDYSSPGGNELHRKIRNNLPDLPIKALDGIGGEYKVSNPFLTLQGTSYVLSLPGNRSQTISTNLLGSFNVRNTALAF
LTGLGLGLDPKGMFSSLKEIPQIPGRFQIVYSKDRSRMAVVDYAHTPDALENIIRSVRNSRPKRLITLFGCGGDRDKTKR
PKMARIAEELSDQVILTSDNPRSEKPEAILDEIQSGFSPGFTPLLREVDRARAISEGVGILPEGGCLLVAGKGHEEYQII
GKEKRHFSDVEEVRKAFGLF
>Mature_500_residues
MKMKLTNLLHEFPELKLGSLPSGKNPDSVPIEYIQSDSRKTNPEDIFCVPESIGTKKGEFISNTKASVILLHRSSNISID
SSKIVLECEEDPEQLQGRIASFLLGHPSKTLEIVAVTGTNGKTSLTNILFALAKDQGKICGLIGTIGVKFGDRSIDTGYT
TPDASSLNLILKQMKDEGVTTVFMEASSHGLKLGRIGGISLKAGVFTNLTQDHLDFHSDMEDYFESKFRLFEILDVSKSP
FAVLDYSSPGGNELHRKIRNNLPDLPIKALDGIGGEYKVSNPFLTLQGTSYVLSLPGNRSQTISTNLLGSFNVRNTALAF
LTGLGLGLDPKGMFSSLKEIPQIPGRFQIVYSKDRSRMAVVDYAHTPDALENIIRSVRNSRPKRLITLFGCGGDRDKTKR
PKMARIAEELSDQVILTSDNPRSEKPEAILDEIQSGFSPGFTPLLREVDRARAISEGVGILPEGGCLLVAGKGHEEYQII
GKEKRHFSDVEEVRKAFGLF

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=417, Percent_Identity=35.4916067146283, Blast_Score=206, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_LEPBJ (Q04V91)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_799937.1
- ProteinModelPortal:   Q04V91
- SMR:   Q04V91
- STRING:   Q04V91
- GeneID:   4409379
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0479
- eggNOG:   COG0769
- HOGENOM:   HBG602753
- OMA:   GHEEGQT
- PhylomeDB:   Q04V91
- ProtClustDB:   CLSK574168
- BioCyc:   LBOR355277:LBJ_0479-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 54801; Mature: 54801

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: BINDING 38-38 BINDING 187-187 BINDING 195-195 BINDING 395-395 BINDING 471-471 BINDING 475-475

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKLTNLLHEFPELKLGSLPSGKNPDSVPIEYIQSDSRKTNPEDIFCVPESIGTKKGEF
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEECCHHCCCCCCCC
ISNTKASVILLHRSSNISIDSSKIVLECEEDPEQLQGRIASFLLGHPSKTLEIVAVTGTN
CCCCCEEEEEEEECCCCEECCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GKTSLTNILFALAKDQGKICGLIGTIGVKFGDRSIDTGYTTPDASSLNLILKQMKDEGVT
CCHHHHHHHHHHHCCCCCEEEEEEHHEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCE
TVFMEASSHGLKLGRIGGISLKAGVFTNLTQDHLDFHSDMEDYFESKFRLFEILDVSKSP
EEEEECCCCCEEEEEECCEEEECCHHHCCCHHHHHHHHHHHHHHHHHHEEEHEEECCCCC
FAVLDYSSPGGNELHRKIRNNLPDLPIKALDGIGGEYKVSNPFLTLQGTSYVLSLPGNRS
EEEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCEEECCCEEEEECCEEEEECCCCCC
QTISTNLLGSFNVRNTALAFLTGLGLGLDPKGMFSSLKEIPQIPGRFQIVYSKDRSRMAV
CEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEE
VDYAHTPDALENIIRSVRNSRPKRLITLFGCGGDRDKTKRPKMARIAEELSDQVILTSDN
EEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEECCC
PRSEKPEAILDEIQSGFSPGFTPLLREVDRARAISEGVGILPEGGCLLVAGKGHEEYQII
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEE
GKEKRHFSDVEEVRKAFGLF
CCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKMKLTNLLHEFPELKLGSLPSGKNPDSVPIEYIQSDSRKTNPEDIFCVPESIGTKKGEF
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEECCHHCCCCCCCC
ISNTKASVILLHRSSNISIDSSKIVLECEEDPEQLQGRIASFLLGHPSKTLEIVAVTGTN
CCCCCEEEEEEEECCCCEECCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GKTSLTNILFALAKDQGKICGLIGTIGVKFGDRSIDTGYTTPDASSLNLILKQMKDEGVT
CCHHHHHHHHHHHCCCCCEEEEEEHHEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCE
TVFMEASSHGLKLGRIGGISLKAGVFTNLTQDHLDFHSDMEDYFESKFRLFEILDVSKSP
EEEEECCCCCEEEEEECCEEEECCHHHCCCHHHHHHHHHHHHHHHHHHEEEHEEECCCCC
FAVLDYSSPGGNELHRKIRNNLPDLPIKALDGIGGEYKVSNPFLTLQGTSYVLSLPGNRS
EEEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCEEECCCEEEEECCEEEEECCCCCC
QTISTNLLGSFNVRNTALAFLTGLGLGLDPKGMFSSLKEIPQIPGRFQIVYSKDRSRMAV
CEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEEE
VDYAHTPDALENIIRSVRNSRPKRLITLFGCGGDRDKTKRPKMARIAEELSDQVILTSDN
EEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEECCC
PRSEKPEAILDEIQSGFSPGFTPLLREVDRARAISEGVGILPEGGCLLVAGKGHEEYQII
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEE
GKEKRHFSDVEEVRKAFGLF
CCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA