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The map label for this gene is ruvC [H]

Identifier: 116329113

GI number: 116329113

Start: 3003632

End: 3004132

Strand: Reverse

Name: ruvC [H]

Synonym: LBL_2536

Alternate gene names: 116329113

Gene position: 3004132-3003632 (Counterclockwise)

Preceding gene: 116329114

Following gene: 116329108

Centisome position: 83.11

GC content: 44.31

Gene sequence:

>501_bases
TTGAGAATCATCGGAATCGATCCGGGTTCTCATCGAGCCGGTTATGCGATTTTGGAAAAAACCGCCTCTAAAATCCGCAT
TTTGGCCTATGGAACCGTGGAGGTTCCTTCGGGAACTCCGAGTCCGAATAATCTTTTGGTATTGCGGAAGGGACTTACGG
AAATTTTGAAAGAGTTCAAACCTTCCATCGCTTCGGTGGAGGAAATGTTTTTTGCGAAAAATAAAAAGACCGCATCAAGA
GTATTCGAGTCAAGGGGAGTTCTGTTAGTTACTTTAGCGGAAATGAATATTCAAATTCTGGAACCTACGGTTTCTCAGAT
TAAAAAAGGAACTACCGGAAGCGGAAACGCGGACAAAAAACAAATCCGTCAAGCTTTAAAACTACTGCTCGATATTGATT
TACTCAAAGGGCACGACGACTCTTGGGATGCCGTTGCCGCGGCCTATGTCGGTCTTTCGATGAGTTCCAGCCCTTTGTTT
GCGGCGCGTTTTAAAGTTTAA

Upstream 100 bases:

>100_bases
ATTCTCCGAAAATCCCCGGTGCCGTTTCCTCTTTGAAAGATAAGGAAGAAGCGGGTTCTTCTTGCTTATCTTTTTTCAAA
TTCGTTCGGGAATTTTTAAC

Downstream 100 bases:

>100_bases
CCGCGAGAGATGCCAATGAGCGACTGACCGCGCTTTTGATATGTCTGAGTTTGTAACGGGACTCGCTGCGAACGTCCAAA
AGAATCACGTCCTCGTTTTC

Product: crossover junction endoribonuclease

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MRIIGIDPGSHRAGYAILEKTASKIRILAYGTVEVPSGTPSPNNLLVLRKGLTEILKEFKPSIASVEEMFFAKNKKTASR
VFESRGVLLVTLAEMNIQILEPTVSQIKKGTTGSGNADKKQIRQALKLLLDIDLLKGHDDSWDAVAAAYVGLSMSSSPLF
AARFKV

Sequences:

>Translated_166_residues
MRIIGIDPGSHRAGYAILEKTASKIRILAYGTVEVPSGTPSPNNLLVLRKGLTEILKEFKPSIASVEEMFFAKNKKTASR
VFESRGVLLVTLAEMNIQILEPTVSQIKKGTTGSGNADKKQIRQALKLLLDIDLLKGHDDSWDAVAAAYVGLSMSSSPLF
AARFKV
>Mature_166_residues
MRIIGIDPGSHRAGYAILEKTASKIRILAYGTVEVPSGTPSPNNLLVLRKGLTEILKEFKPSIASVEEMFFAKNKKTASR
VFESRGVLLVTLAEMNIQILEPTVSQIKKGTTGSGNADKKQIRQALKLLLDIDLLKGHDDSWDAVAAAYVGLSMSSSPLF
AARFKV

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=130, Percent_Identity=30, Blast_Score=82, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 18028; Mature: 18028

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIGIDPGSHRAGYAILEKTASKIRILAYGTVEVPSGTPSPNNLLVLRKGLTEILKEFK
CEEEECCCCCCCCHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHC
PSIASVEEMFFAKNKKTASRVFESRGVLLVTLAEMNIQILEPTVSQIKKGTTGSGNADKK
CCHHHHHHHHHHCCCHHHHHHHHHCCEEEEEEECCCEEEECHHHHHHHCCCCCCCCCCHH
QIRQALKLLLDIDLLKGHDDSWDAVAAAYVGLSMSSSPLFAARFKV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEECC
>Mature Secondary Structure
MRIIGIDPGSHRAGYAILEKTASKIRILAYGTVEVPSGTPSPNNLLVLRKGLTEILKEFK
CEEEECCCCCCCCHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHC
PSIASVEEMFFAKNKKTASRVFESRGVLLVTLAEMNIQILEPTVSQIKKGTTGSGNADKK
CCHHHHHHHHHHCCCHHHHHHHHHCCEEEEEEECCCEEEECHHHHHHHCCCCCCCCCCHH
QIRQALKLLLDIDLLKGHDDSWDAVAAAYVGLSMSSSPLFAARFKV
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA