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The map label for this gene is paaJ-2 [H]

Identifier: 116329089

GI number: 116329089

Start: 2971249

End: 2972460

Strand: Direct

Name: paaJ-2 [H]

Synonym: LBL_2507

Alternate gene names: 116329089

Gene position: 2971249-2972460 (Clockwise)

Preceding gene: 116329082

Following gene: 116329090

Centisome position: 82.2

GC content: 47.19

Gene sequence:

>1212_bases
ATGTCAAACGCCTATGTAATCGATGCGGTCAGAACCCCGAGAGGGAAAGGAAAAAAAAGAGGGGCCCTGGCGAGCATTCA
CCCGCAAGAACTCTCCGCTGCAACCTTAAATGCGATCCAAGAAAGAAACGGAATCAAGCCTGAAATAGTAGAAGAAGTCG
TTATGGGATGCGTATCTCAAGTGGACGATCAAGCGGCTTGCATTGCGCGTTATGCGGTCATGTCTGCACTCTGGCCGAAC
TCGGTCCCCGGTTATACCGTAAACCGTTTCTGCGGTTCCGGACTTCAAGCGGTCAACAATGCCGCTAATCATGTTCAATC
CGGTTCCATGCAAATCGCCCTCGGAGGTGGAGTCGAATCAATGTCCCGCGTAAAGATGGGAGCCGATATGAACGGAAGAG
ACTTTAATATCGGAAATCCGAATATTCAAAAACACTATAATTTGGTGCCTCAAGGGATCTCTGCGGATTTGATCGCGACT
AAGTTCGGAATTTCCCGCGAAGAAGCGGATCGTTTTGCGGAATCATCCCAAATCAAAGCGGACAAAGCGATCAAAGAAGG
TTACTTCAAAAAATCCATCGTCCCGGTTAAAATCGAGGATGGTACTATAGTCGATACGGATGAAAATCCGAGAATCGAAT
CCACTTTCGAATGGCTCTCCGATTTGGCTCCGGTTTTCAAAACGATCGGTGAAAAGGAATTGGATGCGATTGCACTTAAA
TCCTACCCGGAAATTCCGAAGATCAATCACATACATACGTTAGGCAACTCTTCCGGAATAGTAGACGGTGCGGCTTCGGT
TCTTCTTGCTTCGGATGAAGGGATCAAAAAATACGGACTCAAACCTCGGGCGAAAATTGTCGCGATGGCTTCCACAGGTG
AAGACCCTACGATCATGTTAACAGGGCCGGTCTCCGCTTCTAAAAAAGCGTTAGCAATAGCCGGACTCAAGGCCGACGAT
ATCGATCTTTGGGAAATCAACGAAGCGTTTGCTTCTGTGGTTCTTTACACTCAGAAATCGCTCAATATTCCTTTGGAAAA
AATCAACGTCAACGGAGGTTCGATTTCTCTCGGACATCCTCTTGGAGCAACCGGTGCGATTCTTCTTGGGACCGCATTAG
ACGAGCTTGAAAGGAGACAAAAGCGTTATGCGCTTATTACTCTTTGTATCGGAGGAGGAATGGGAATCGCGACAATCATA
GAAAGAATGTAG

Upstream 100 bases:

>100_bases
CAAACTCCTTGGCTTCTCAAAAATAGAAACCGGAATATCCAAAATCACTAAACAGCTGTTCAATTTAATCCTCAGAGAAA
ATCGGCAGCGAGGTAACACA

Downstream 100 bases:

>100_bases
ATTCTTTTTTTCATTCGGGCCGATTTTTATTTTCGGCCCGAATCTTTTGACATTTCTCGCTTTTATTTTTTTATTTCCCA
CTTTTTTGAAGAAATAGAGC

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSNAYVIDAVRTPRGKGKKRGALASIHPQELSAATLNAIQERNGIKPEIVEEVVMGCVSQVDDQAACIARYAVMSALWPN
SVPGYTVNRFCGSGLQAVNNAANHVQSGSMQIALGGGVESMSRVKMGADMNGRDFNIGNPNIQKHYNLVPQGISADLIAT
KFGISREEADRFAESSQIKADKAIKEGYFKKSIVPVKIEDGTIVDTDENPRIESTFEWLSDLAPVFKTIGEKELDAIALK
SYPEIPKINHIHTLGNSSGIVDGAASVLLASDEGIKKYGLKPRAKIVAMASTGEDPTIMLTGPVSASKKALAIAGLKADD
IDLWEINEAFASVVLYTQKSLNIPLEKINVNGGSISLGHPLGATGAILLGTALDELERRQKRYALITLCIGGGMGIATII
ERM

Sequences:

>Translated_403_residues
MSNAYVIDAVRTPRGKGKKRGALASIHPQELSAATLNAIQERNGIKPEIVEEVVMGCVSQVDDQAACIARYAVMSALWPN
SVPGYTVNRFCGSGLQAVNNAANHVQSGSMQIALGGGVESMSRVKMGADMNGRDFNIGNPNIQKHYNLVPQGISADLIAT
KFGISREEADRFAESSQIKADKAIKEGYFKKSIVPVKIEDGTIVDTDENPRIESTFEWLSDLAPVFKTIGEKELDAIALK
SYPEIPKINHIHTLGNSSGIVDGAASVLLASDEGIKKYGLKPRAKIVAMASTGEDPTIMLTGPVSASKKALAIAGLKADD
IDLWEINEAFASVVLYTQKSLNIPLEKINVNGGSISLGHPLGATGAILLGTALDELERRQKRYALITLCIGGGMGIATII
ERM
>Mature_402_residues
SNAYVIDAVRTPRGKGKKRGALASIHPQELSAATLNAIQERNGIKPEIVEEVVMGCVSQVDDQAACIARYAVMSALWPNS
VPGYTVNRFCGSGLQAVNNAANHVQSGSMQIALGGGVESMSRVKMGADMNGRDFNIGNPNIQKHYNLVPQGISADLIATK
FGISREEADRFAESSQIKADKAIKEGYFKKSIVPVKIEDGTIVDTDENPRIESTFEWLSDLAPVFKTIGEKELDAIALKS
YPEIPKINHIHTLGNSSGIVDGAASVLLASDEGIKKYGLKPRAKIVAMASTGEDPTIMLTGPVSASKKALAIAGLKADDI
DLWEINEAFASVVLYTQKSLNIPLEKINVNGGSISLGHPLGATGAILLGTALDELERRQKRYALITLCIGGGMGIATIIE
RM

Specific function: Subunit Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=415, Percent_Identity=35.9036144578313, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI4501853, Length=394, Percent_Identity=39.5939086294416, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI148539872, Length=413, Percent_Identity=38.0145278450363, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI4557237, Length=410, Percent_Identity=31.4634146341463, Blast_Score=185, Evalue=8e-47,
Organism=Homo sapiens, GI4504327, Length=445, Percent_Identity=30.7865168539326, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI194353979, Length=95, Percent_Identity=50.5263157894737, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI48994986, Length=412, Percent_Identity=40.0485436893204, Blast_Score=268, Evalue=5e-73,
Organism=Escherichia coli, GI1787663, Length=425, Percent_Identity=38.5882352941176, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI87082165, Length=416, Percent_Identity=37.0192307692308, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1788554, Length=419, Percent_Identity=38.9021479713604, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1788683, Length=427, Percent_Identity=26.9320843091335, Blast_Score=131, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI133906874, Length=415, Percent_Identity=35.1807228915663, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI25147385, Length=411, Percent_Identity=32.1167883211679, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17535921, Length=411, Percent_Identity=30.9002433090024, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17551802, Length=447, Percent_Identity=28.4116331096197, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=28.641975308642, Blast_Score=128, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6322031, Length=410, Percent_Identity=37.0731707317073, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6325229, Length=423, Percent_Identity=31.2056737588652, Blast_Score=156, Evalue=6e-39,
Organism=Drosophila melanogaster, GI17648125, Length=411, Percent_Identity=36.2530413625304, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24655093, Length=417, Percent_Identity=37.410071942446, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24640423, Length=412, Percent_Identity=33.7378640776699, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI17137578, Length=444, Percent_Identity=29.5045045045045, Blast_Score=164, Evalue=9e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42987; Mature: 42856

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAYVIDAVRTPRGKGKKRGALASIHPQELSAATLNAIQERNGIKPEIVEEVVMGCVSQ
CCCCEEEEEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VDDQAACIARYAVMSALWPNSVPGYTVNRFCGSGLQAVNNAANHVQSGSMQIALGGGVES
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCHHH
MSRVKMGADMNGRDFNIGNPNIQKHYNLVPQGISADLIATKFGISREEADRFAESSQIKA
HHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHH
DKAIKEGYFKKSIVPVKIEDGTIVDTDENPRIESTFEWLSDLAPVFKTIGEKELDAIALK
HHHHHHCCHHHCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SYPEIPKINHIHTLGNSSGIVDGAASVLLASDEGIKKYGLKPRAKIVAMASTGEDPTIML
CCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCHHHHCCCCCCEEEEEECCCCCCEEEE
TGPVSASKKALAIAGLKADDIDLWEINEAFASVVLYTQKSLNIPLEKINVNGGSISLGHP
ECCCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHEECCCCCCCEEEEECCCCEEEECCC
LGATGAILLGTALDELERRQKRYALITLCIGGGMGIATIIERM
CCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHCC
>Mature Secondary Structure 
SNAYVIDAVRTPRGKGKKRGALASIHPQELSAATLNAIQERNGIKPEIVEEVVMGCVSQ
CCCEEEEEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VDDQAACIARYAVMSALWPNSVPGYTVNRFCGSGLQAVNNAANHVQSGSMQIALGGGVES
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCHHH
MSRVKMGADMNGRDFNIGNPNIQKHYNLVPQGISADLIATKFGISREEADRFAESSQIKA
HHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHH
DKAIKEGYFKKSIVPVKIEDGTIVDTDENPRIESTFEWLSDLAPVFKTIGEKELDAIALK
HHHHHHCCHHHCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SYPEIPKINHIHTLGNSSGIVDGAASVLLASDEGIKKYGLKPRAKIVAMASTGEDPTIML
CCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCHHHHCCCCCCEEEEEECCCCCCEEEE
TGPVSASKKALAIAGLKADDIDLWEINEAFASVVLYTQKSLNIPLEKINVNGGSISLGHP
ECCCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHEECCCCCCCEEEEECCCCEEEECCC
LGATGAILLGTALDELERRQKRYALITLCIGGGMGIATIIERM
CCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: Acyl group transfer; Claisen condensation [C]

Inhibitor: Tris (hydroxy methyl) aminomethane [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]